The world’s first bacterial minesweeper gameCOLISWEEPER

ETH Zurich 2013

From Minesweeper* to Colisweeper

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1.5 cm

*Démineur

Flagging Solution

FP

Analogous Game States

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1 2 Playing Solution

Game

Over 1 2

Implementation: Biological Circuit

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luxI

nagZ

Pconst

Pconst

AHL

luxR

Pconst

gusA

PLuxR (high sensitivity)

aes

PLuxR (low sensitivity)

phoAchromosomal

Mine Cell / Sender Non Mine Cell / Receiver

AHL

1

Results

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ACCTGTAGGATCGTACAGGT ACNNNTAGGATCGTANNNGT

R2 > 0.95

21

PhoAPhoA+GusA

PhoA+GusA+AesPhoA+NagZ

2

OutputSignal Processing

Aes GusA NagZ

Magenta Butyrate

Salmon Glucoronide

X-GluNac

Gradient

 

Proof of Principle

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AHL

Time: 11 h

# GFP RFP1 ü

2 ü

3 ü ü

RFP [au]

luxR

PLac

gfp

PLuxR (high)

luxI

Pconst

rfp

PLuxR (low)

GFP Expression A: Low - 1 mine nearby B: Medium - 2 mines nearby C: High - 3 mines nearby D: None - 0 mines nearby Mine producing AHL

A

B

CD

Colisweeper - Tool for Education

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Synthetic Biology I !Lab Course

luxR

PLac

gfp

PLuxR (high)

rfp

PLuxR (low)

Acknowledgments

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Prof. Sven Panke Prof. Yaakov Benenson Prof. Jörg Stelling !!!!!!!!

Markus Jeschek Gaspar Morgado Sabine Österle Laura Prochazka Benjamin Häfliger Bartolomeo Angelici Lukas Widmer !!!!

Dr. Johannes Härle Andreas Bosshart Anke Gehringer Niels Bürckert Verena Jäggin Thomas Horn Urs Senn

Sponsors

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Merci pour votre attention!

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Questions?

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iGAM4ER 2013

Proof of principle - GFP

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