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Broughton High School 1 Biology Student Workbook 2015 Mr. Davis Name____________________________________________________ Period: _________________ December Daily Performance Rubrics Each Item is worth 2 points for a total of 10 points possible per day - This will be a TEST grade! Date : __________ ___On Time to Class ___Have Biology Notebook ___Start Do Now Quickly and Quietly ___Have Pencil and Blank Paper ___Have Homework Ready to Turn In ___Total ________Sign off To Do Now Question __________________________________________________________________ __________________________________________________________________ __________________________________________________________________ __________________________________________________________________ Answer: Date : __________ ___On Time to Class ___Have Biology Notebook ___Start Do Now Quickly and Quietly ___Have Pencil and Blank Paper ___Have Homework Ready to Turn In ___Total ________Sign off To Do Now Question __________________________________________________________________ __________________________________________________________________ __________________________________________________________________ __________________________________________________________________ Answer: Date : __________ ___On Time to Class ___Have Biology Notebook ___Start Do Now Quickly and Quietly ___Have Pencil and Blank Paper ___Have Homework Ready to Turn In ___Total ________Sign off To Do Now Question __________________________________________________________________ __________________________________________________________________ __________________________________________________________________ __________________________________________________________________ Answer: Date : __________ ___On Time to Class ___Have Biology Notebook ___Start Do Now Quickly and Quietly ___Have Pencil and Blank Paper ___Have Homework Ready to Turn In ___Total ________Sign off To Do Now Question __________________________________________________________________ __________________________________________________________________ __________________________________________________________________ __________________________________________________________________ Answer: Date : __________ ___On Time to Class ___Have Biology Notebook ___Start Do Now Quickly and Quietly ___Have Pencil and Blank Paper ___Have Homework Ready to Turn In ___Total ________Sign off To Do Now Question __________________________________________________________________ __________________________________________________________________ __________________________________________________________________ __________________________________________________________________ Answer:

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Broughton High School 1

Biology Student Workbook 2015 Mr. Davis

Name____________________________________________________ Period: _________________

December Daily Performance Rubrics

Each Item is worth 2 points for a total of 10 points possible per day - This will be a TEST grade!

Date : __________

___On Time to Class

___Have Biology Notebook

___Start Do Now Quickly and Quietly

___Have Pencil and Blank Paper

___Have Homework Ready to Turn In

___Total

________Sign off

To Do Now Question

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Answer:

Date : __________

___On Time to Class

___Have Biology Notebook

___Start Do Now Quickly and Quietly

___Have Pencil and Blank Paper

___Have Homework Ready to Turn In

___Total

________Sign off

To Do Now Question

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Answer:

Date : __________

___On Time to Class

___Have Biology Notebook

___Start Do Now Quickly and Quietly

___Have Pencil and Blank Paper

___Have Homework Ready to Turn In

___Total

________Sign off

To Do Now Question

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Answer:

Date : __________

___On Time to Class

___Have Biology Notebook

___Start Do Now Quickly and Quietly

___Have Pencil and Blank Paper

___Have Homework Ready to Turn In

___Total

________Sign off

To Do Now Question

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Answer:

Date : __________

___On Time to Class

___Have Biology Notebook

___Start Do Now Quickly and Quietly

___Have Pencil and Blank Paper

___Have Homework Ready to Turn In

___Total

________Sign off

To Do Now Question

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Answer:

Broughton High School 2

Biology Student Workbook 2015 Mr. Davis

Broughton High School 3

Biology Student Workbook 2015 Mr. Davis

Broughton High School 4

Biology Student Workbook 2015 Mr. Davis

Chapter 10 Vocabulary Words – Cell Growth & Cell Division

1. Cell Division

2. Mitosis

3. Cytokinesis

4. Chromatid

5. Centromere

6. Interphase

7. Cell Cycle

8. Prophase

9. Centriole

10. Spindle

11. Metaphase

12. Anaphase

13. Telophase

14. Cyclin

15. Cancer

Broughton High School 5

Biology Student Workbook 2015 Mr. Davis

Chapter 12 Vocabulary Words – DNA & RNA

1. Transformation

2. Nucleotide

3. Base Pairing

4. Bacteriophage

5. Chromatin

6. Histone

7. Replication

8. DNA Polymerase4

9. Gene

10. Ribosomal RNA

11. Messenger RNA

12. Transfer RNA

13. RNA Polymerase

14. Promoter

Broughton High School 6

Biology Student Workbook 2015 Mr. Davis

Chapter 12 Vocabulary Words – DNA & RNA

15. Intron

16. Exon

17. Codon

18. Translation

19. Anticodon

20. Mutation

21. Point Mutation

22. Frame shift Mutation

23. Polyploidy

24. Operon

25. Operator

26. Differentiation

27. Hox gene

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Biology Student Workbook 2015 Mr. Davis

Broughton High School 8

Biology Student Workbook 2015 Mr. Davis

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Biology Student Workbook 2015 Mr. Davis

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Biology Student Workbook 2015 Mr. Davis

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Biology Student Workbook 2015 Mr. Davis

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Biology Student Workbook 2015 Mr. Davis

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Biology Student Workbook 2015 Mr. Davis

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Biology Student Workbook 2015 Mr. Davis

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Biology Student Workbook 2015 Mr. Davis

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Biology Student Workbook 2015 Mr. Davis

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Biology Student Workbook 2015 Mr. Davis

Broughton High School 18

Biology Student Workbook 2015 Mr. Davis

MITOSIS WORKSHEET Name:

Matching: match the term to the description (drag/drop or write the letter)

_____ 1. The sister chromatids are moving apart.

_____ 2. The nucleolus begins to fade from view. _____ 3. A new nuclear membrane is forming around the chromosomes.

_____ 4. The cytoplasm of the cell is being divided.

_____ 5. The chromosomes become invisible.

_____ 6. The chromosomes are located at the equator of the cell.

_____ 7. The nuclear membrane begins to fade from view. _____ 8. The division (cleavage) furrow appears.

_____ 9. The chromosomes are moving towards the poles of the cell.

_____ 10. Chromatids line up along the equator.

_____ 11. The spindle is formed.

_____ 12. Chromosomes are not visible. _____ 13. Cytokinesis is completed.

_____ 14. The cell plate is completed.

_____ 15. Chromosomes are replicated.

_____ 16. The reverse of prophase.

_____ 17. The organization phase.

D. Metaphase E. Anaphase C. Telophase B. Interphase A. Prophase D. Metaphase E. Anaphase C. Telophase B. Interphase A. Prophase D. Metaphase E. Anaphase C. Telophase B. Interphase A. Prophase D. Metaphase E. Anaphase C. Telophase B. Interphase A. Prophase D. Metaphase E. Anaphase C. Telophase B. Interphase A. Prophase D. Metaphase E. Anaphase C. Telophase B. Interphase A. Prophase D. Metaphase E. Anaphase C. Telophase B. Interphase A. Prophase D. Metaphase E. Anaphase C. Telophase B. Interphase A. Prophase D. Metaphase E. Anaphase C. Telophase B. Interphase A. Prophase D. Metaphase E. Anaphase C. Telophase B. Interphase A. Prophase D. Metaphase E. Anaphase C. Telophase B. Interphase A. Prophase D. Metaphase E. Anaphase C. Telophase B. Interphase A. Prophase D. Metaphase E. Anaphase C. Telophase B. Interphase A. Prophase D. Metaphase E. Anaphase C. Telophase B. Interphase A. Prophase D. Metaphase E. Anaphase C. Telophase B. Interphase A. Prophase D. Metaphase E. Anaphase C. Telophase B. Interphase A. Prophase

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Biology Student Workbook 2015 Mr. Davis

Fill in the blank: (drag/drop or write the word) Some will be used more than once.

________________18. What phase are daughter cells in as a result of mitosis?

________________19. During what phase of mitosis do centromeres divide and the chromosomes move toward their respective poles?

________________20. What is the phase where chromatin condenses to form chromosomes?

________________21. What is the name of the structure that connects the two chromatids?

________________22. In a chromosome pair connected by a centromere, what is each individual chromosome called?

________________23. What are the two parts of cell division?

________________24. What structure forms in prophase along which the chromosomes move? ________________25. Which phase of mitosis is the last phase that chromatids are together?

________________26. Which phase of the cell cycle is characterized by a non-dividing cell?

________________27. What structure is produced when protein fibers radiate from centrioles?

________________28. What forms across the center of a cell near the end of telophase?

________________29. The period of cell growth and development between mitotic divisions? ________________30. What is the phase where cytokinesis occurs?

E. Anaphase

C. Telophase

D. Metaphase

B. Interphase

A. Prophase

K. Cell Plate

I. Mitosis

J. Spindle fiber

H. Cytokinesis

G. Chromatid

F. Centromere C. Telophase

A. Prophase

E. Anaphase

D. Metaphase

F. Centromere

K. Cell Plate

I. Mitosis

J. Spindle fiber

H. Cytokinesis

G. Chromatid

E. Anaphase

J. Spindle fiber

C. Telophase

B. Interphase B. Interphase B. Interphase

Broughton High School 20

Biology Student Workbook 2015 Mr. Davis

DNA & Mitosis Worksheet

1. A strand of mRNA has a base sequence of GUAAGAC. Which of the following DNA strands coded for this mRNA? A. TCGGTGA

B. GTAAGAC C. CATTCTG

D. GUAAGAC

2. Which of the following structures is found in ribonucleic acid, but not in deoxyribonucleic acid? A. guanine

B. uracil C. thymine D. cytosine

3. Consider the following diagram:

4. What stage of protein synthesis is taking place at part II? A. replication of DNA

B. translation of mRNA C. replication of tRNA D. transcription of mRNA

Use the diagram of protein synthesis to answer questions 4 and 5.

5. What process is shown? A. transcription

B. translation C. duplication

D. replication

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Biology Student Workbook 2015 Mr. Davis

DNA & Mitosis Worksheet

6 What type of molecule is number 3? A. tRNA B. mRNA

C. rRNA D. amino acid

The following diagram represents a small

segment of the DNA molecule. Use it to answer the following questions 6 and 7.

7. Molecules 5 and 6 are A. amino acids and nucleotides

B. sugar and nitrogen bases C. sugar and phosphates D. oxygen and nucleotides

8. Molecules 1, 2, 3 and 4 are called? A. lipids B. sugars

C. phosphates D. nitrogen bases

9. When preparing for cell division, the chromatin condenses and becomes a

A. gene B. chromosome C. protein

D. codon

10. The molecule that transports the code of information from DNA to the ribosome is A. tRNA

B. rRNA C. mRNA D. a amino acid

11. A mutated gene alters or inhibits the production of the proper A. nitrogenous bases B. protein

C. ribosome D. lipid

5

6

5

2 1

4 3

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Biology Student Workbook 2015 Mr. Davis

DNA & Mitosis Worksheet

12. Chromosomes line up on spindles in the middle of the cell during

A. B. C. D.

13. Body cells have two sets of chromosomes, one from the mother and one from the father. These pairs of matched chromosomes are called A. clones

B. gametes C. mutations

D. homologous chromosomes

14. During meiosis, why is the chromosome number reduced to half? A. to maintain the species original chromosome number at fertilization B. to make sexual reproduction easier with fewer chromosomes

C. with a reduced number of chromosomes, the chances of a mutation decreases D. to increase the variation of individuals during reproduction

15. The following is a brief diagram showing chromosome number during mitosis.

What will the chromosome number be for each cell in level III?

I.

II.

III.

A. 8

B. 16 C. 32 D. 64

16. What cell process would occur prior to a cell going through mitosis or meiosis?

A. replication B. reproduction

C. translation D. transcription

17. What is the key difference between mitosis and meiosis during the actual process? A. Both double the number of chromosomes, but in meiosis the cell divides twice.

B. In mitosis the number of chromosomes doubles twice and then divides. C. In meiosis the number of chromosomes does not double, and the cell divides.

D. In mitosis the number of chromosomes doubles and then the cell divides once.

16

32

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Biology Student Workbook 2015 Mr. Davis

DNA & Mitosis Worksheet

17. What is the function of mitosis? A. to make egg or sperm cells B. to maintain the chromosome number in the offspring

C. to produce more DNA for protein synthesis D. to produce more body cells

18. Why is sexual reproduction usually considered more advantageous than asexual reproduction?

A. It maintains the same exact DNA from one generation to the next. B. It helps to increase genetic variation.

C. It promotes more interaction between males and females of the same species. D. It helps to maintain the chromosome number of the species.

19. Which of the following groups of molecules makes up a DNA nucleotide? A. ribose, adenine, phosphoric acid

B. deoxyribose, uracil, phosphoric acid C. ribose, thymine, phosphoric acid

D. deoxyribose, adenine, phosphoric acid

20. In a DNA molecule, where does a mutation occur? A. in the deoxyribose B. in the nitrogen bases

C. in the phosphoric acid D. in the hydrogen bonds

Broughton High School 24

Biology Student Workbook 2015 Mr. Davis

What is the structure of DNA?

Background Information:

A DNA molecule looks like a twisted ladder. A DNA molecule is made up of chains of sugar groups and phosphates connected by pairs of nitrogen bases. The letters A, T, C, and G stand

for the four different nitrogen bases.

Materials: scissors, colored paper in six different colors, tape

Procedure: 1. Cut out the DNA parts. Below is a checklist of the parts you will need:

12 sugar molecules – light green paper

12 phosphate molecules – light blue paper 3 A’s – green paper 3 T’s – red paper

3 C’s – blue paper 3 G’s – purple paper 2. Construct the two sides of the DNA molecule with the sugars and phosphates.

3. Construct the molecule of DNA by fitting base pairs along the sides of the sides of the molecule.

4. Tape the entire DNA molecule together.

Questions: (Yes, you will have many points taken off if you do not

answer these questions!)

1. Name the two molecules which alternate to form the sides (uprights) of a DNA molecule. 2. Name the specific molecule to which each base is attached.

3. Name (no abbreviations) the four nitrogen bases found in DNA. 4. Show the sequence or code of the nitrogen bases in your model. Give both sides.

5. Only two combinations of base pairs are possible. What are they? 6. Want is another name for the twisted ladder?

7. Name the four scientists who discovered the structure of DNA? 8. Are all of the DNA models in the class exactly the same? If not what are the differences?

9. What do you need to do o your model to make it look like a real DNA molecule? 10. Complete the following piece of DNA:

_______________________________________

A T C G A A C T G G T C A T | | | | | | | | | | | | | | | | |

________________________________________← Insert the complimentary bases here.

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Biology Student Workbook 2015 Mr. Davis

Directions: Place a check mark in the correct column for either DNA or RNA or Both.

Similarities and Differences Between DNA and RNA

DNA RNA

Deoxyribonucleic acid

Ribonucleic acid

Ribose present

Deoxyribose present

Phosphate group (phosphoric acid) present

Adenine present

Thymine present

Uracil present

Guanine present

Cytosine present

Formed from nucleotides

Double stranded

Single stranded

Remains in nucleus

Moves out of nucleus

Contains a chemical message or code

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Biology Student Workbook 2015 Mr. Davis

For the following examples, what is the appropriate anti codon and amino acid produced by the codons?

Anticodon Amino Acid

1. UGG ___________ ____________________

2. GCA ___________ ____________________

3. GCU ___________ ____________________

4. CAU ___________ ____________________

5. AUC ___________ ____________________

Broughton High School 27

Biology Student Workbook 2015 Mr. Davis

Mitosis in Onion Root Tip Cells

I. TITLE: Mitosis and Cell Division in Plants

II: Background:

The genetic information of plants, animals, and other eukaryotic organisms resides in several individual DNA molecules or

chromosomes . Each Human cell possesses 46 chromosomes, while each cell of an onion possesses 8 chromosomes. All cells must

replicate their DNA when diving. During DNA replication, the two strands of the DNA double helix separate, and for each original

strand a new complementary strand is produced, yielding two identical DNA molecules. DNA replication yields an identical pair of

DNA molecules called sister chromatids attached at a region called the centromere.

III: The stages of Mitosis:

Prophase: during prophase, the chromosomes super-coil and the fibers of the spindle apparatus begin to from between the

centromeres. The nuclear membrane disappears in the cytoplasm.

Metaphase: The chromosomes line up in the middle of the cell.

Anaphase: The centromeres split and the sister chromatids begin to move to opposite ends of the cell

Telophase: The chromosomes at the opposite ends of the cell begin to cluster together. This is also when cytokinesis occurs, leading

to separate cell.

IV. PURPOSE: (1) to study the process of Mitosis and determine what occurs in each phase. (2) to estimate how long a cell spends in each stage.

V. Lab Objective:

To better understand the process and stages of mitosis

To use a light compound microscope to examine Onion Root Tips

VI: PROCEDURE:

1. Obtain a prepared slide of onion root tip. Under low power locate a group of cells near the tapering end of the root tip. Make note that there are three root tips on your slide; you will be using each one of them. Move to high power and find a good section with many cell divisions. Make sure the field of view is completely filled with the cells.

2. We will use 50 as an estimate of the total number of cells in the high power field of view. Find this number in the three columns beside "CELL TOTAL" at the bottom of the data table.

3. Count the number of cells in each phase of mitosis and record the numbers in the column labeled "1". A good way to do this is for each lab partner to count independently and compare estimates, then take the average of the two. Interphase numbers can be calculated by subtracting the other four counts from the 50.

DATA TABLE:

Phases Root Tip #1 Root Tip #2 Root Tip #3 Average Class Average

Prophase

Metaphase

Anaphase

Telophase

Interphase

Total Cells

50 50 50 50 50

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Biology Student Workbook 2015 Mr. Davis

VII: Observations: Scan the microscope under 10X objective. Look for the region that has large nuclei relative to the size of the cell.

Switch to the 40 X objective to make closer observations. Record your observations in the table provided.

Label each Phase of the Onion Cell:

__________________________________________________________

__________________________________________________________

__________________________________________________________

__________________________________________________________

__________________________________________________________

__________________________________________________________

__________________________________________________________

VIII. Results:

Find and draw a cell showing each stage of mitosis

Prophase Metaphase Anaphase Telophase

Broughton High School 29

Biology Student Workbook 2015 Mr. Davis

Activity 1: DNA Model

Sweet Candy Model DNA

Candies: Strawberry Twizzlers

Spice Drops

4 toothpicks

Hand Wipe

1. Take the two candy cords - assign one color to represent the pentose sugar molecules and the other to represent the phosphate molecules. Cut the candy cords into 2 - 3 centimeter pieces.

2. Using the needle and thread string half the candy pieces together lengthwise alternating the two colors to form a chain.

3. Repeat step 2 with the remaining half of candy pieces to form a second chain of the same length.

4. Lay the two chains down side by side so pieces of the same color are opposite one another

5. Count the number of pentose sugar molecules you have in one chain (you should have the same number in both chains). Obtain this number of cocktail sticks. These represent hydrogen bonds that hold the base pairs together.

6. Divide the sweets into four different colors. Assign names to the each of the four colors to represent the nucleotide bases - adenine, cytosine, guanine or thymine.

7. The bases have to be paired up on the cocktail sticks. Adenine always pairs with thymine and guanine with cytosine, so make sure you get the right colors matching.

8. Push each end of a cocktail stick into candy pieces representing pentose sugar molecules lying opposite one another - the cocktail sticks should join the two chains together so they look like the rungs of a ladder

9. Hold the end of each chain and twist slightly to get the double helix effect for your DNA model

The Secret of Life

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Biology Student Workbook 2015 Mr. Davis

Strawberry DNA Extraction

Introduction:

DNA is found in cells from Animals and Plants. DNA is a double stranded macromolecule composed of nucleotide bases pairing

Adenine with Thymine and Guanine with Cytosine. DNA can be extracted from cells by a simple technique with household

chemicals, enabling students to see strands of DNA with the naked eye. This simple DNA extraction lesson plan lets students t ry a fun

DNA activity while they learn more about this important biology topic. Students will try extracting DNA from a banana by following

the simple instructions and can take the activity further with the use of a microscope. Give it a try and enjoy fun classroom science for

kids. Found in the shape of a double helix, DNA or deoxyribonucleic acid as it is otherwise known provides the blueprint for various

forms of life. This includes humans, monkeys and even things like bananas. Let's try a fun activity that involves extracting DNA from

a strawberry.

Purpose: To extract DNA from the fruit of a strawberry plant.

1. Blend 2 strawberries together with 1 cup of water until smooth

2. Fill tubes with around 15mls of the strawberry and water solution, making one for each student in your class. Add around 10

drops of detergent to the strawberry mixture in the tubes

3. Put a lid on the tubes and gently shake them (this will break open the cells and release the DNA)

4. Carefully pour around 20 to 30mls of methylated spirits (also known as denatured alcohol) into the strawberry mixture a

straightened paperclip or similar object to fish out the gooey strands of strawberry DNA.

Safety Precautions: Do not eat or drink in the laboratory.

Wear Apron & Safety Goggles.

Materials / Equipment (per student group): Heavy duty zip-lock baggie

1 strawberry (fresh or frozen and thawed)

Cheesecloth

Funnel

100 ml beaker

Centrifuge Tube

wooden coffee stirrer

DNA Extraction Buffer

Ice-cold 95% ethanol or 95% isopropyl alcohol

Procedure: 1. Place one strawberry in a zip lock baggie and carefully press out all of the air and seal the bag.

2. Smash the strawberry with your fist for 3 minutes.

3. Add 10 ml extraction buffer to the bag and carefully press out all of the air and seal the bag.

4. Mush again for 2 minute2.

5. CAUTION: Mix carefully; the fewer bubbles created the better your results.

6. Filter through cheesecloth in a funnel into beaker. Support the test tube in a test tube rack.

7. Discard the extra mashed strawberry.

8. Pour filtrate into test tube so that it is 1/8 full.

9. Slowly pour the ice-cold alcohol into the tube until the tube is half full and forms a layer over the top of the strawberry

extract.

10. At the interface, you will see the DNA precipitate out of solution and float to the top. You may spool the DNA on your

wooden stick or pipette tip.

11. Spool the DNA by dipping a pipette tip or glass rod into the tube right where the extract layer & alcohol are in contact with

each other. With your tube at eye level, twirl the rod & watch as DNA strands collect .

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Broughton High School 32

Biology Student Workbook 2015 Mr. Davis

Practice Test No. 5 Multiple Choice

1. What shape does a DNA molecule have if it comes from a natural ( not artifical) Christmas tree? 1. Candy-cane-shaped 2. Double helix 3. Reindeer-shaped 4. Star-shaped

2. A nucleotide does not contain? 1. A 5-carbon sugar 2. A Polymerase protein 3. a nitrogen base 4. a phosphate group

3. In prokaryotes, DNA molecules are located in the 1. nucleus 2. ribosome 3. cytoplasm 4. Histone

4. The first step of DNA replication is that the DNA ________. 1. translates 2. unzips 3. disappears 4. terminates

5. The main enzyme involved in breaking the hydrogen bonding between DNA nucleotides is? 1. tRNA 2. ribose 3. RNA Polymerase 4. DNA Polymerase

6. The site for the production of proteins within a cell is the: 1. Centriole 2. nuclei 3. ribosomes 4. vacuoles

7. Each nucleotide is composed of a 5 carbon sugar, a nitrogen base, and 1. an amino acid 2. a fatty acid 3. an alcohol 4. a phosphate group

8. What process occurs when the DNA code is made into protein? 1. replication 2. transcription 3. translation

4. mitosis

9. In messenger RNA, each codon specifies a particular? 1. nucleotide 2. purine 3. amino acid 4. Pyrimidines

Base your answers to questions 10 and 11 on the

diagram below.

A – T A – T

G – C

C – G

T – A G - C

DNA Strand 1 DNA Strand 2

10. The number of amino acids coded by strand 1 is? 1. 1 2. 2 3. 8 4. 12

11. Which represents the sequence of nitrogen bases in the molecule of mRNA synthesized by DNA strand 1? 1. T-T-C-G-U-C 2. A-A-C-G-T-C 3. U-U-C-G-A-C 4. A-A-G-C-U-G

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12. The phase of mitosis during which chromosomes become visible and the centrioles separate from one another is ? 1. prophase 2. anaphase 3. metaphase 4. telophase

13. In eukaryotes, DNA molecules are located in the 1. nucleus 2. ribosome 3. cytoplasm 4. Histone

14. The last step of DNA replication is that the DNA ________. 1. translates 2. unzips 3. sealed together by DNA ligase 4. folds together to form a double helix

15. According to the table below, which amino acid sequence would most likely be determined by a section of a DNA molecule with the base sequence C-C-G-A-A-G-G-A-T?

Messenger RNA

Codon

Amino Acid

AGA Arginine

CUA Leucine

GGC Glycine

UUC Phenylalanine

1. Glycine – Phenylalanine-leucine 2. Glycine- arginine-leucine 3. Glycine-leucine-arginine 4. Phenylalanine- leucine -glycine

16. What did Hershey and Chase’s work show? 1. Genes are probably made of DNA 2. Genes are probably made of protein 3. Genes are made of both DNA & protein 4. Viruses contain DNA but not protein 5. Bacteria contain DNA but not protein

17. ________ can never leave the nucleus. 1. DNA 2. RNA 3. protein 4. spindle fiber

18. The four nitrogen bases of DNA are 1. adenine, guanine, thymine, uracil 2. adenine, guanine, cytosine, thymine 3. uracil, guanine, cytosine, thymine 4. adenine, guanine, cytosine, uracil

19. What process occurs when the DNA code makes a copy of itself? 1. replication 2. transcription 3. translation

4. mitosis

20. The DNA code is copied onto a strand of messenger RNA through the process of what? 1. replication 2. transcription 3. translation 4. transaction

21. What is the sugar component of the RNA molecule? 1. adenine 2. ribose 3. deoxyribose 4. glucose

22. Which of the following nucleotide(s) bond(s) with adenine? 1. thymine only 2. uracil only 3. thymine and uracil 4. thymine, uracil, and cytosine

23. What determines the genetic information carried in DNA? 1. order of the nucleotides 2. shape of the DNA 3. location in the nucleus 4. position of the phosphate groups

24. Base pairing in DNA is limited to adenine-thymine and what other pair?

1. guanine-cytosine 2. guanine-adenine 3. cytosine-adenine 4. cytosine-thymine

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25. Which molecule transports amino acids to the ribosomes? a. protein molecules b. transfer RNA (tRNA) c. messenger RNA (mRNA) d. chromosomes

26. Anticodons are part of the structure of a _____? 1. DNA 2. mRNA 3. tRNA 4. rRNA 5. proteins

27. If the segment of DNA represented by the diagram below was used as a template (a pattern) in the synthesis of mRNA, which sequence represents the order of bases that could be found in the mRNA molecule?

DNA Segment: A-A-T-G-C-T

1. T-T-A-G-C-A 2. U-U-A-C-G-U 3. T-T-U-G-C-T 4. U-U-A-C-G-A

28. What best describes the shape of the DNA molecule? 1. ladder 2. double helix 3. biome 4. square

29. DNA controls the production of what? 1. proteins 2. lipids 3. sugars 4. hydrolysis

30. Which of the illustrations below represents

metaphase of mitosis?

31. Which of the following would not be found in the nucleus? 1. nucleotides 2. mRNA 3. tRNA 4. DNA

32. The individuality of an organism is determined by the organisms? 1. amino acids 2. DNA nucleotide sequence molecules 3. transfer RNA 4. nitrogenous bases

33. Deoxyribose is 1. an amino acid 2. a sugar 3. a phosphate 4. a nitrogen base

34. Which molecule is composed of two complementary chains of nucleotides? 1. ATP 2. DNA 3. RNA 4. amino acid chain

35. Which is the correct order of information transfer in the cell? 1. translation-transcription-replication 2. replication-translation-transcription 3. replication- transcription-translation 4. transcription-replication-translation

36. This scientist was responsible for using X-ray crystallography to take a picture of DNA: 1. Watson and Crick 2. Rosalind Franklin 3. Griffith 4. Chase

37. Which scientists won a Nobel Prize for their discovery of the structure of the DNA molecule? 1. Watson and Franklin 2. Chargaff and Wilkins 3. Watson and Crick 4. Wilkens and Franklin

38. Which is not a step in the replication of DNA: 1. DNA unzips 2. Bases pair up on an open strand 3. Backbone attaches 4. RNA binds to the DNA

Broughton High School 35

Biology Student Workbook 2015 Mr. Davis

39. Which statement is true about both DNA and RNA? 1. They are both single-stranded 2. Uracil is one of the nitrogen bases 3. They contain guanine 4. They are composed of ribose

40. The main enzyme involved in linking individual nucleotides into DNA molecules is? 1. Transfer RNA 2. DNA Ligase 3. RNA Polymerase 4. DNA Polymerase

41. Changes in the DNA sequence that affects genetic information are known as: 1. replications 2. mutations 3. transformations 4. prokaryotes

42. Purine nucleotides have _____________. 1. one ring 2. three rings 3. two rings 4. no rings

43. The three types of RNA include: ____________

1. mRNA, tRNA, pRNA 2. tRNA, rRNA, mRNA 3. mRNA, dRNA, tRNA 4. tRNA, rRNA, pRNA

44. During replication, which sequence of nucleotides would bond with the DNA sequence TATGA?

1. TATGA 2. UAUGA 3. ATACT 4. AUAGA 5. ATACA

45. Anticodons are part of the structure of:______ 1. DNA 2. messenger RNA 3. transfer RNA 4. ribosomal RNA 5. proteins

Extra Credit Questions

46. In photosynthesis , plants use carbon dioxide, water , and light to produce: 1. carbon 2. water 3. glucose 4. chlorophyll

47. To obtain energy and use cellular energy, plants use: 1. Photosynthesis only 2. Photosynthesis and cellular respiration 3. Cellular respiration only 4. Neither photosynthesis nor cellular respiration

48. Cellular respiration takes place inside: 1. An animal cell only 2. A plant cell only 3. Both plant & animal cells 4. Neither plant nor animal cell

49. What are two reactants of aerobic respiration? 1. C6H12O6 and O2 2. CO2 and chlorophyll 3. CO2 and O2 4. H2O and chlorophyll

50. Which type of cellular respiration produces the most cellular energy? 1. Aerobic respiration 2. Anaerobic respiration 3. Alcoholic respiration 4. Lactic acid fermentation

The following equation refers to questions 51 & 52.

The equation below represents a summary of a biological process:

CO2 + H2O + glucose + energy

51. The process above is known as: 1. cellular respiration 2. photosynthesis 3. fermentation 4. glycolysis

52. The process above is completed in which organelle? 1. mitochondria 2. ribosomes 3. cell membranes 4. chloroplasts

Broughton High School 36

Biology Student Workbook 2015 Mr. Davis

53. Label each section of the diagram of Transcription and Translation.

54. Describe the process of Transcription in the above diagram: __________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

55. Describe the process of Translation in the above diagram: _______________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

37 Broughton High School

Biology Student Work book 2015 Mr. Davis

Extra Credit 1. Use complete sentences to describe and label each component involved in DNA Replication.

A. _______________________________________________________________________________________________

B. ____________________________________________________________________________________________ ___

C. _______________________________________________________________________________________________

D. _______________________________________________________________________________________________

E. _______________________________________________________________________________________________

F. _______________________________________________________________________________________________

G. _______________________________________________________________________________________________

Replication Steps

Step 1:

____________________________________________________________________________________________________________

___________________________________________________________________________________________________________

Step 2

____________________________________________________________________________________________________________

___________________________________________________________________________________________________________

Step3:

____________________________________________________________________________________________________________

___________________________________________________________________________________________________________

Step 4:

____________________________________________________________________________________________________________

___________________________________________________________________________________________________________

Step 5:

____________________________________________________________________________________________________________

____________________________________________________________________________________________________________

3’ 5’

A

B

C

D E

F

G 3’ 5’ Word Box

DNA Polymerase

RNA Polymerase

Complementary DNA

Original DNA strand

Nitrogenous Bases

Hydrogen Bonding

lagging strand - Okazaki

fragments