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My classical and favorite welcome presentation for 2nd yr LM (females), UQU.
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Welcome to the Macroworld of Microbiology
Second year Lab Medicine (Females)1433- 1434 A.H. / 2012- 2013
Microbiology Practical (Course I)
By:
Mrs. Hibah Abdul-Wahab I. Abu-Sulaiman
What are the life forms?
Humans.Animals.Plants.
– And…
Microbes
4 WH Questions
• What are microbes?
• Where do they live?
• When do they survive?
• Why they are participating other life forms?
So the study of microbes is called…
Microbiology
Why do we study microbiology??
Microbes have dominated Earth for more than 3 billion years.
Why do we study medical microbiology???
Nine out of ten cells in our bodies are microbes
So, why are we not suffering from any disease all the time?
When microbes can cause a disease?
Weak immune system.Pathogenic microbial invasion.Invasion to a sterile body site.
Objectives
Types of microbes.Definition of microbes.A sample story in bacteriology lab.
Microbes Include…
Bacteria.Viruses.Parasites.Fungi.
Bacteria
• Unicellular prokaryotes.
• Examples:– Staphylococcus spp.– Vibrio cholera.
Viruses
• Smallest infectious particle.
• Example:– Rotavirus.
Parasites
• Eukaryotes, unicellular or multicelluar.
• Example:– Giardia lambia.
Fungi
• Eukaryotes, in a unicellular or filamentous form.
• Example:– Trichophyton rubrum
An ending story…
Please…
Don’t sleep
Once upon a time…
Hinds’ 12th birthday
In the next day…
She went to A Gastroenterologist
A Stool Sample Was Requested
In Microbiology Lab
In the Next Day
And After Reading the Culture Media
And Performing the Biochemical Tests
It was Escherichia coli
The Microbiologist Decided to
Perform Antibiotic Susceptibility Testing (AST)
In the Next Day of AST
After 3 Days of Medication
Am I right when I said the Macroworld of Microbiology
When someone speaks about another,
What is the first thing that comes into your mind??!
Face
Is it…?
Rectangular face
Spherical face
Elongated face
Square shaped face
Triangular face
His Behavior
Does he like to work in a group, alone or…?
So now after knowing these two things, you can speak
to him/ her
They live and behave like we do in our society
Objectives
• To define different bacterial morphologies.
• To define their arrangements together.
Face
Is it…?
Rectangular faceSpherical face
Elongated face
Bacteria are exactly the same…
Spherical face
Elongated face
Rectangular face
What about their arrangements?!
How does the Turkish pilgrims look like in their groups?
Accordingly…
Staphylococci
Staphylococci Staff
How does the Malaysian pilgrims look like in their groups?
Accordingly…
Streptococci
Streptococci strip
In pairs
Diplococci
The same is applied for bacilli
Spirilla
Is there any other type?!
Vibrio
Some are flagellated
Hmm, did you like bacteria?!
Lets revise their morphologies and arrangements
Before entering
The Microbiology Lab
Be sure of following the safety rules
Safety in Microbiology Laboratory
Aims of following safety rules and practices
• To prevent the occurrence of accidental infections in the lab and reduce them if they occur.
• Prevent potentially harmful microbes from leaving the lab.
Safety in the Microbiology Lab
• Wash your hands with soap and water before you begin and after you finish your work and before leaving the lab.
• Decontaminate your work surface before and after you work
Safety in the Microbiology Lab
• No bags inside the lab.
Safety in the Microbiology Lab
• Wear only closed-toed shoes in the laboratory.
Safety in the Microbiology Lab
• Wear lab scrub and fully- buttoned lab coat.
Safety in the Microbiology Lab
Safety in the Microbiology Lab
• Tight your hair.• No cosmetics
• If you are:– Injured…– Immunocompromised..– Pregnant…– Allergic to latix…
Safety in the Microbiology Lab
• Be careful not to burn yourself when using Bunsen burner.
Safety in the Microbiology Lab
• Keep your hands away from your mouth and eyes…
Safety in the Microbiology Lab
• Do not eat, drink or chew gum while you are in the lab
Safety in the Microbiology Lab
• Avoid using electronic devices …– Mobiles, MP3 players,
laptops…
Safety in the Microbiology Lab
• Never mouth pipetting.
Safety in the Microbiology Lab
• Always keep cultures in a test tube rack whether you are working at your bench or walking about in the lab.
Safety in the Microbiology Lab
• Place all contaminated tubes, plates and waste materials in appropriate receptacles for subsequent sterilization.
Safety in the Microbiology Lab
• If you spill a chemical on your skin, immediately wash the affected area. If a chemical or a culture gets into your eye, immediately wash your eye for 5 min.
Safety in the Microbiology Lab
• If anything containing viable bacteria spills, cover the spell or broken glass with a paper towel and a generous amount of disinfectant to remain on the slip for at least 20 min. before cleaning it up.
Safety in the Microbiology Lab
• Sweep up pieces of broken glass (after disinfection if necessary) and place them in the specified container.
Safety in the Microbiology Lab
• Place sharps, such as syringes and slides, in a designated sharp containers.
Safety in the Microbiology Lab
• Do not keep cultures in your lab drawer or locker when you have finished with them.
• Do not remove cultures, reagents, or other materials from the lab unless you have permission to do so.
Safety in the Microbiology Lab
• Know the first aid kit location in your lab.
Safety in the Microbiology Lab
Recommended Book
Stay Tuned
To Contact Me
• Register on FB:
Hibah’s microclasses
Note: please send your name and Gp#
• e-mail: [email protected]
Thank you for paying attention
Any Questions???