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www.plexdb.org
Data Mining at PLEXdb : Plant and Plant Pathogen Gene Expression Database
www.plexdb.org
Sudhansu Dash1,2,3, Hong Lu5, Ethalinda KS Cannon3,5, Nick Lauter3,4, Volker Brendel5, Roger P Wise4, Julie A Dickerson1,2
1Virtual Reality Application Center; 2Dept. of Electrical & Computer Engg.; 3Crop Genome Informatics Lab; 4Dept. of Plant Pathology and Corn Insects and Crop Genetics Research, USDA-ARS; 5GDCB. Iowa State University.
• Supports all Affymetrix microarrays for plants and plant pathogens including annotation.
• MIAME/Plant compliant data submission.• Experiment Visibility: private / collaborators and
reviewers / public. • Uses standard ontologies.• Links to PlantGDB, Gramene, GrainGenes, UniProt, etc.• Option to submit data at NCBI GEO
• Data from 217 microarray experiments, > 6000 chips• Experiments from 14 Affymetrix arrays.• Species: Arabidopsis, Barley, Citrus, Fusarium, Grape,
Maize, Medicago, Poplar, Rice, Soybean, Sugarcane, Tomato, Wheat.
PLEXdb Features:
DATA MINING: The Microarray Platform Translator tool
Translates probe sets of one microarray chip to another in a different species.
DATA MINING: Gene OScilloScopeSearch for experiments where expression of queried genes fluctuate (oscillate) the most.
Co-expressed genes or profile neighbors can be downloaded and viewed as heatmap or expression graph
DATA MINING: Find co-expressed genes
GENELIST SUITE: Tools to Create GeneLists
Fold change
Treatment specific expression
GO category
GENELIST SUITE: Analyze GeneLists
Partitional clustering based on K Means
GENELIST SUITE: Annotate a GeneList
TOOLS: Map Interesting Genes from Your Analysis on the rice/Arabidopsis Genome
Model Genome Interrogator
Get the gene model, promoter, 5’UTR sequences, etc.
VISUALIZATION: Emphasis on data exploration
Expression Graph of probe set OsAffx.26001.3.S1_at in experiment OS2 across hybridizations:
Repressed in salt stress condition.
VISUALIZATION: Expression of an individual gene
CONTROL SALT STRESS