BIOLOGY PROJECT

Created By :

Anggarjuna P. Puji Putra - 03

Aristya P. Marzuki - 04

Mirza B. Firnanda - 18

XII SCIENCE 6

SMAN 5 SURABAYA

2011-2012

Nutrient Testing on Food

A. Goal

To know the nutrition of the our daily food.

B. Tools and materials

- Sugar - Blue Band - Avocado

- Soya Bean - Rice - Meat

- Bread - Orange - Milk

- Peanut - Tofu - Cooking Oil

- Lugol - Fehling A - Fehling B

- Biuret - Reaction Tube - Baker Glass

- Pipette - Filtration Paper - Drop plate

C. How to work

1. Test the Amilum/starch concentration of foods :

- First materials food to be tested must be crushed or dissolved

- Then Squirt Food Samples to be tested into the plate drops using a pipette

- Squirt Food Samples would be tested with Lugol's

- Observe what happens to the food sample

2. Test the Glucose concentration of foods:

- First materials food to be tested must be crushed or dissolved

- Then Squirt Food Samples (5 drops) to be tested into the plate drops using a pipette

- Squirt Food Samples would be tested with Fehling A (3 drops) and Fehling B (3 drops)

- Heat the solution on it while swaying laterally right and left

- Observe what happens to the food sample

3. Test the Protein concentration of foods:

- First materials food to be tested must be crushed or dissolved

- Then Squirt Food Samples to be tested into the plate drops using a pipette

- Squirt Food Samples would be tested with Biuret Solution

- Observe what happens to the food sample

4. Test the Fat concentration of foods:

- First materials food to be tested must be crushed or dissolved

- Then Squirt Food Samples to be tested into the plate drops using a pipette

- Wait until drops samples are drying

- Observe what happens to the food sample

D. Result of Experiment

1. Test the Amilum concentration of foods:

- There are several food samples which when dropped with Lugol then the color changed to

blue-black. This means the food contains amilum

Amilum experiment table result:

No Food sample Color Explanation

1 White of egg Orange Doesn’t contain amilum

2 Milk Yellow Doesn’t contain amilum

3 Meat Orange Doesn’t contain amilum

4 Tempe Brown Doesn’t contain amilum

5 Orange Yellow Doesn’t contain amilum

6 Peanut Light purple Containingamilum

7 Rice Old purple Containingamilum

8 Tofu Old purple Containingamilum

9 Avocado Yellow-green Doesn’t contain amilum

10 Bread Old purple Containingamilum

11 Cooking oil Orange Doesn’t contain amilum

12 Butter Orange Doesn’t contain amilum

13 Sugar Yellow Doesn’t contain amilum

2. Test the Glucose concentration of foods:

- There are several food samples which when dropped with Fehling A and Fehling

B, then heat that, if the color changed to red brick or orange. This means the food

contains glucose.

Glucose experiment table result:

No Food sample Color Keterangan

1 White of egg Black Doesn’t contain glucose

2 Milk Orange Contain glucose

3 Meat Purple Doesn’t contain glucose

4 Tempe Purple Doesn’t contain glucose

5 Orange Oranee Contain glucose

6 Peanut Purple Doesn’t contain glucose

7 Rice Green Doesn’t contain glucose

8 Tofu Purple Doesn’t contain glucose

9 Avocado Brown-yellow Contain glucose

10 Bread Orange Contain glucose

11 Cooking oil Green Doesn’t contain glucose

12 Butter Green Doesn’t contain glucose

13 Sugar Orange Contain glucose

3. Test the Protein concentration of foods:

- There are several food samples if the drops with the biuret color changed to a

purplish redcolor. This means the food contains protein.

Protein experiment table result :

No Food sample Color Explanation

1 White of egg Purple Contain Protein

2 Milk Purple Contain Protein

3 Meat Purple Contain Protein

4 Tempe Mauve Contain Protein

5 Orange Black Doesn’t contain protein

6 Peanut Purple Contain Protein

7 Rice Blue Doesn’t contain protein

8 Tofu Purple Contain Protein

9 Avocado Biru Doesn’t contain protein

10 Bread Brown Doesn’t contain protein

11 Cooking oil Blue Doesn’t contain protein

12 Butter Brown Doesn’t contain protein

13 Sugar Blue Doesn’t contain protein

4. Test the Fat concentration of foods:

- There are a few samples of our food is applied to filter paper, if there is scar then he contain

fat.

Fat experiment table result:

No Food sample Spot/blot Explanation

1 White of egg There is Contain fat

2 Milk There is Contain fat

3 Meat There is Contain fat

4 Tempe No Doesn’t contain fat

5 Orange No Doesn’t contain fat

6 Peanut There is Contain fat

7 Rice No Doesn’t contain fat

8 Tofu No Doesn’t contain fat

9 Avocado There is Contain fat

10 Bread No Doesn’t contain fat

11 Cooking oil There is Contain fat

12 Butter There is Contain fat

13 Sugar No Doesn’t contain fat

E. Conclusion

1. If there are food that dropped lugol change the color become old blue, these contain

starch. if the color doesn’t change, that food doesn’t contain starch. From that

experimentresult, the food that contain starch is: rice, bread, tofu, and peanut

2. If there are food dropped with fehling A and fehling B then burn and shake change that

color into red brick or orange, these contain glucose or sugar. From the experiment

result, we can found the food contain glucose is: Milk, Orange, Avocado, bread, and

sugar.

3. When we drop some biuret in food that contain protein, the color will be change into

purple lil bit red. From result above, the food contain protein is: White of egg, milk, meat,

tempe, peanut, and tofu.

4. When we put some food with our finger then polished on filter paper and dried it up, it

may cause 2 things. First, if we see that paper was become transparent that food contain

fat if not, these doesn’t contain. From the experiment, the food contain fat is: White of

egg, milk, meat, peanut, avocado, cooking oil, and butter.

Effect of Body Weight Against Respiration in Animals and Plants

A. Purpose Observe the relation of body weight on the speed of respiration in animals/plants by using a simple respirometer.

B. Whats you need: A cricket 2 gr sprout Weight Scale

A respirator board 3 gr KOH(s)

5 ml red eosin Cotton Vaseline

C. How to works

1. Weigh the cricket that will tested into scale 2. Packs 3gr solid KOH with cotton. Be carefull, if the KOH interact with our hand may

cause irtation. Then, put that and the cricket into respiration tube. 3. Pair the tube with respiration pipe by rubbing Vaseline on the connection area. Its

purpose to prevent entry of air. 4. After all is installed on respirator boards, close the edge of the pipe respirator use

your fingers for about 1 minute. It aims to crickets spend the oxygen(O2) in the respirator tubes and pipes in order to get perfect calculation.

5. After O2 inside tube and pipe probably used up, inject the red eosin into respirator pipe with until 0 scale. Then watch closely what happen!

6. Do the same way with the sprout.

D. Result of experiment

Minutes to - The distance of eosin movement

Cricket 1.07 gr Sprout 2 gr

1 0.5 0.03

2 0.7 0.03

3 0.8 0.04

4 0.7 0.02

5 0.7 0.04

Average per minutes

0.72 0.32

1. Chart of eosin speed movement

2. From the graphic, we can conclude that cricket respiration is faster than sprout although sprout more heavier. This happened because the body activity that need oxygen of cricket is more complex than sprout.

3. Role of solid KOH is to absorb CO2 result of respiration. 4. Eosin can move because CO2 result of respiration absorb by KOH. So, air volume

inside tube is decreasing and suck the eosin. 5. Beside of body weight, respiration speed is also depended on body activity and

metabolism. If there are lot of activity, more O2 is required.

E. Conslusion From the experiment above, we can conclude that respiration speed each

individual is relative. Its depended on body weight, activity, and metabolism. Fat man needs more air than thin man. In other example, when you are running, your breath becomes faster. It means your body need a lot oxygen. That is the opposite when you are sleeping, doesn’t require a lot of air.

0.05 0.12

0.2 0.27

0.34

0.05 0.07 0.08 0.07 0.07

1 2 3 4 5

Dis

tan

ce(i

nch

)

Minutes to-

Cricket (1.07 gram)

eosin movement Δ each minutes

0.03 0.06

0.1 0.12

0.16

0.03 0.03 0.04 0.02

0.04

1 2 3 4 5

Dis

tan

ce(i

nch

)

Minutes to-

Sprout (2 gram)

eosin movement Δ each minutes