Ethnopharmacology and Antioxidant Activity

of Dryobalanops aromatica

Gunawan Pasaribu

The Center for Research and Development

on Forest Engineering and Forest Product Processing

Dryobalanops aromatica

Good quality wood

NTFPs: oils, gums and crystal

Oils and crystals have been utilized since 851 AD

Kapur/kamper wood

Biomedicine:

The Arabian medicine states that kapur crystal can be

used as a gall remedy; inflammation of the liver; teeth;

eye medication.

In the Persian medicine, it was used for nosebleeds;

cool the kidneys and bladder, heart disease and

excessive lust.

Kapur crystal can also strengthen the heart by

Armenian Medicinal Science.

Antioxidants

to protect our body

from free radicals. Natural sources: vegetables, fruit (tocoferol, ascorbic acid, carotenoid, poliphenol dan flavonoid), green tea (polyphenol), milk (amino acid), fish and vitamin.

Free radicals is an unstable

atomic or molecule.

Free radicals destroy a tissue

What is free radicals ?

Subulussalam

Singkil

Pakpak Bharat

Tapteng

Exploration...

Objectives

to elaborate the ethnopharmacological and

antioxidant activity of Dryobalanops aromatica

Ethnophar macology

• Ethopharmacology study was conducted on the aplication of kapur that used as a remedy by locals comunity. The method used is in-depth interviews with some of the local people

Phytochemical testing

• The testing was done on crude extract of Dryobalanops aromatica (wood, leaves, and crystal). The phytochemical compound was qualitatively examined in the crude extract included flavonoid, tannin, saponin, triterpenoid, steriod, hydroquinon and alkaloid

Antioxidant activity

• The free radical scavenging activity of the extract was based on the scavenging activity of the stable 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical that was determined by Blois (1958) method

Methodology

DPPH Method for Antioxidant Activity Analysis

Extract made in 5, 7.5,

10, 15, 25, 50 and 75 ppm

add 500 µl DPPH 1mM in methanol

and tare with methanol up to 5 ml

Incubated at 370 C temperatures

during 30 minutes

Absorption was measured with UV-VIS

spectrophotometer at 515 nm wavelength

IC 50

Stock solution 1000 ppm

in metanol

The free radical scavenging activity of the extract, base on the

scavenging activity of the stable 1,1-diphenyl-2-picrylhydrazyl (DPPH)

free radical was determined by method described by Blois (1958).

Result and Discussion

The oils as one of the NTFPs items from this

species have been used a lot by local people as

medicine to cure health problems, such as

wound healing, liniment, stomachache, catching-

cold remedy, and toothache. The oils is applied

by rubbing the oil on the pain body.

Ethopharmacology study

Qualitative phytochemical testing

Compound Sample

wood leaves crystal

Flavonoids + + +

Tannins + + +

Saponins + + +

Triterpenes - + +

Steroids - - -

Hydroquinone + + +

Alkaloids:

Dragendorf

Wagner

Meyer

-

-

-

-

-

-

-

-

-

Antioxidant Activity Testing

No Sample IC50 (ppm)

1

2

3

Wood

Leaves

Crystal

6.54

16.05

372.25

Standar (Vitamin C) 4.87

CONCLUSIONS

Ethnopharmacology study revealed that the oils as one of the NTFPs items from this species have been used a lot by local people as medicine to cure human-health troubles, such as wound healing, liniment, stomachache, catching-cold remedy, and toothache

Based on the result of testing antioxidant activity, it can be concluded that the Dryobalanops aromatica has the potency as natural antioxidant source

Thank you