Environmental Mngmnt Systems Bioprocesses

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    If we consider the biosphere in terms of the turnover of elements rather than in

    terms of energy flow, then cyclical patterns are observed.Life on earth depends on (1) chemical recycling; it is also dependent on (2) one-

    way energy flow through the biosphere.

    Summary of some critical problems that can occur in an ecological system:

    1.

    Disruption of essential chemical cycles on a global or local scale:a. Breaking the Cycle; e.g., desertification, global warming and change of climate

    b. Changing the rate of cycling by chemical overloads or leaks in the cycle. e.g.,upsetting oxygen and carbon cycle by deforestation, dumping industrial wastes in

    lakes and rivers

    2. Disruption of energy flow on a local or global scale:a. Decreasing or increasing solar energy input by changing the properties of the

    local or global atmosphere. E.g., green house gases and ozone depleting chemical

    release into atmosphere.

    b. Heat or entropy build up in the environment due to use of too much energy,large scale combustion of fossil fuels for electricity generation.- we cannot ignore

    the second law of thermodynamics.

    Rapid evolution of human society took place in recent ten thousand years. Several

    developments have made an immense consequence on the natural environment due to

    human activities. In the last two hundred years, it has been observed that the use ofenergy resources on a large scale affects the general flow of matter in the biosphere

    contributing to disturbances in natural cycles, beyond earths bearing capability.

    In early stage of hunting, primitive agriculture and with skillful use of tools,manual and animal nutrition derived power was the limiting energy source. Organic

    molecules generated through photosynthesis in plants with solar energy as the source of

    energy, provided food for herbivorous animal species (including humans) and animaid development. Resource depletion and pollution became a real possibility.

    The efficiency of energy conversions in nature far exceeds that of Man designed

    production processes. A living organism not only produces materials it needs to functionand in doing this uses energy in a highly efficient way. In times of a positive energy

    balance energy is stored in compounds such as starch, glycogen and lipids. Each living

    organism degrades bio-molecules that have fulfilled their biological function to smaller

    units and subsequently uses these for the production of new bio-molecules or as a cellularfuel. Microorganisms- built-in integrated recycling can after the death of the organism,

    use the bio-molecules present in an organism. The non-bio-gradable nature of

    manufactured products such as synthetic plastics, may cause problems by accumulationin the environment.

    Integral Life Cycle management:

    Mankind is withdrawing fossil energy and raw materials from the earths reserves to formaking products for fulfilling social needs. During the process of manufacture, wastes

    and degraded energy may be released to environment and after usage the product may

    become a disposable material in the environment. Recycle of material can involve somemore energy input.

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    When we make a choice of a product, a consideration of the total impact on environment

    of producing it, using it and finally handling it as waste should be made. If its utility isless than the adverse impact then we should forgo the use of it.

    In addition to considering economic feasibility of a process in a situation, energy and

    environmental factors are also satisfactory; society can support the product both from

    producer and consumer point of view.

    Biotechnology: New Revolution:Biotechnology is the application of organisms, biological systems or biological processes

    to manufacturing and service industries. It is based on understanding of biosciences and

    process engineering and involves handling of bio-molecules that occur in nature.Here we consider applications of biotechnology in agriculture, chemical synthesis

    and energy management. Two strategies for the use of biotechnology are studied (i) To

    reduce the environmental problems arising from conventional technology. (ii) To replace

    existing environmentally damaging technology.Recalcitrant organic molecules and inorganic pollutants: Compounds that persist in the

    environment are called recalcitrant. Abiotic organic chemicals in water, soil etc.are noteasy to treat as these are not metabolized easily. However in some cases selectivedevelopment of mutants have given biotechnology solution to these problems. Inorganic

    heavy metal pollution too has been tackled by bioprocess developments. REFER:

    Microbial biosorbents: Meeting the challenge of heavy metal pollution in aqueoussolutions Current Science, v 78, No 8, April 2000,(Review Paper,967-973)

    Man made compounds that are found in unusually high concentrations in the environment

    are called xenobiotic. These do not get degraded easily by microbes and accumulate in

    the environment. Considerable research is being done on his topic by environmentalbiotechnologists.

    2. Waste, Pollution--need for Treatment

    Interaction: Man & EnvironmentNature of Wastes & Pollutants

    Environmental impacts of release

    Treatment: End of pipe vs process integrated technology

    Landfill technology for solid waste

    Waste generation is the byproduct of consumption and production activities and

    tends to rise with the level of economic advance. Wastes arise from domestic andindustrial activity, e.g., sewage, wastewater, agriculture and food waste from food

    processing, wood wastes and ever increasing range of toxic industrial chemical products

    and byproducts. Costs for properly dealing with waste are escalating and much attentionis presently devoted to efficient and effective waste management, which will include

    costs of collection, storage, processing and removal of wastes.

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    A Tale Of Too Crowded Cities

    Urban India is stretching itself out like naan "with bulges here and there, thin at the

    edges''. Shapeless, running out of land, water, even clean air. A landscape ripe for the

    kind of mayhem Delhi has witnessed this past years. The Sunday Times tracked thecorridors of urban expanse, and chaos. Almost a third of India - over 300 million people -

    already lives in its towns and cities. Said Union urban development minister Jagmohan,"Urban India today is as large (in numbers) as the total India was in 1947. In numbers, we

    will have the second largest urban population in the world, next to China. We are just not

    paying attention.''The urban population is expected to hit the 500 million mark in the next two decades.

    More worryingly, the growth is uneven. India already has the highest congestion rate in

    the world -- about 44 per cent of families in urban areas live in just one room. In the

    nation's Capital alone, more than a third of all residents live in slums, without propershelter, drinking water, sanitation, or access to health care and good education.

    Thousands of illegal colonies had emerged and thousands of illegal industriesrecently rose in revolt against attempts at control, given a voice by politicians seekingshort-term dividends. Planning has been thrown to the winds, bringing to the fore

    questions of urban governance and its definition, the nature of planning, the cost

    of services, public versus private sector involvement in development.

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    NATURE OF WASTES:

    GASLIQUID

    SOLID

    CONCENTRATED & LOCALISED

    DILUTE & DISPERSEDBIODEGRADABLE

    RECALCITRANTMIX OF BIODEG. & RECALCI.

    HAZARDS:BIOLOGICAL

    CHEMICAL

    PHYSICAL

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    To dispose solid wastes a recent method is landfill. Landfill practices vary from countryto country. One of the common approaches is the use of the cell emplacement strategy. In

    this the collected refuse is covered on all sides by soil at the end of each working day.

    There is some degree of stratification. The size of the cells depends on the daily volume

    that is tipped. Each cell is compressed and roughly leveled by mechanical bulldozers.Generally the depth of cell is limited to about 2.5 meters. Depth of the soil used to cover

    at the end of each day is about 20cm.

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    3. Aerobic Wastewater Treatment

    Aerobic wastewater treatment process is essentially a biologically based process, which islong established. There are two main types of treatment; fixed film processes and

    homogeneous growth processes. The most widely used fixed film process is the trickling

    filter bed, and for homogeneous growth methods, the activated sludge process. Otherspecialized treatment regimes exist, and are often applied to the treatment of specific

    industrial waste.

    The choice of a treatment process is aided by the ability to define the nature of hewastewater to be processed by the use of established parameters. These include BOD,

    COD, ammoniacal nitrogen and suspended matter.

    Knowledge of fundamental equations and processes ultimately enable the design of

    vessels to handle known quantities of wastewater of a known composition and theoptimization of these stages for the removal of BOD or other desired effects, when they

    have been built are operated. This understanding also enables the process to be run cost

    effectively for example in the choice of oxygenation methods in dispersed systems.The skills needed within a wastewater treatment plant are multidisciplinary and

    include civil engineering, Chemical engineering, electrical engineering and obviously

    micro-biology. A biotechnologist should appreciate the integration of these disciplinesand have some understanding of their individual contributions.

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    4. Anaerobic Wastewater Treatment

    WHY BIOGAS IN VILLAGES ?

    ENERGY RECOVERY: BIOGAS SUBSTITUTES FORFUELWOOD & KEROSENE.

    HYGIENIC DISPOSAL OF ANIMAL WASTE CONSERVATION OF FERTILIZER VALUE

    HOW IS GAS FORMED?

    MIXED CULTURE OF BACTERIA DECOMPOSESVOLATILE SOLIDS & GROWS IN ANAEROBIC

    CONDITION; GAS MIXTURE RELEASED

    ADVANTAGES:

    RESULTING SLURRY FREE FROM PATHOGENSCAN BE USED IN COMPOST PIT FOR MANURE

    MILD CONDITIONS: 30o C, pH 6.8-7.2, FEED ONCE ADAY

    FOR COOKING, BURNER, LIGHTING: MANTLELAMP AVAILABLE; FOR A DUAL FUEL ENGINE:

    EASY GAS PURIFICATION FEASIBLE

    FOR RURAL FARM OR FAMILY SIZE PLANT,SUBSIDY AVAILABLE.

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    COMMONLY USED FEED FOR BIOMETHANATION:

    ANIMAL WASTES, URBAN WASTES, and FOOD & AGRO-

    INDUSTRY WASTES.

    MICROBIOLOGICAL ASPECTS OF BIOMETHANATION

    The biomethanation of organic matter in water is carried out in absence of dissolved

    oxygen and oxygenated compounds like nitrate and sulphate. The mixed groups of

    bacteria are naturally occurring in the cow dung slurry and decomposition in three stagesfinally produces a gas mixture of methane and carbon dioxide. Initially larger molecules

    are hydrolysed to simpler molecules which in turn are decomposed to volatile fatty acids

    like acetic acid, propionic acid etc. by a second set of bacteria. Methane forming bacteriacan convert acetic acid, hydrogen and carbon dioxde and produce methane.

    WET ORGANIC WASTE AS FEED FOR BIOGAS PLANT

    ANIMAL WASTES: Excreta of cow, pig, chicken etc

    MANURE, SLUDGE: Canteen and food processing waste, sewage

    MUNICIPAL SOLID WASTE: After separation of non-degradable

    WASTE STARCH & SUGAR SOLUTIONS: Fruit processing, brewery,

    press mud from sugar factory etc

    OTHER INDUSTRIAL EFFLUENTS (B O D): pulp factory waste

    liquor, leather industry waste, coal washery wastewater etc.

    HYDROLYSIS OF BIOPOLYMERS TO MONOMERS

    CONVERSION OF SUGARS, AMINO ACIDS, FATTY ACIDS TO HYDROGEN,

    CO2, AMMONIA AND ACETIC, PROPIONIC AND BUTIRIC ACIDS

    CONVERSION OF H2, CO2, ACETIC ACID TO CH4 AND CO2 MIXTURE

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    Volumetric methane rate in cubic meter gas per cubic meter of digester volume

    V = (Bo So / HRT)[1- K / (HRT*m-1+K)]Bo =Ultimate methane yield in cubic meters methane (Varies from 0.2 to 0.5)So = Influent volatile solids concentration in kgVS/m

    3

    (Loading rate range = 0.7 to 25 kg VS/m3

    d)HRT = Hydraulic retention time in days

    K = Dimensionless kinetic parameter, for cattle dung, K= 0.8+ 0.0016e0.06 So

    m = Maximum specific growth rate of the microorganism in day-1

    Dry and wet fermentation:

    Reference: Solid state anaerobic digestion of cattle dung and agro-residues: Perspectiveand prospects M.Shyam, Journal of Solar Energy Society of India, 10(1): 11-25 (2000)

    WET FERMENTATION MEANS FEED HAS SUBSTRATE TOTALSOLID CONCENTRATION, ( TSC) OF 8 TO 9 %

    DRY FERMENTATION OR SOLID STATE FERMENTATION HASFEED SUBSTRATE TOTAL SOLID CONCENTRATION, ( TSC) OF 20

    TO 30 %, A MIX OF COW DUNG AND A WIDE VARIETY OF AGRO-

    RESIDUES.

    ANAEROBIC DIGESTION OF CATTLE DUNG AND MANY AGRO-RESIDUES AT INITIAL CONCENTRATIONS OF TSC BETWEEN 16 TO

    25 % HAS BEEN DEMONSTRATED SATISFACTORILY IN SMALLBATCH TYPE AND PLUG FLOW TYPE DIGESTERS.

    AT INITIAL TSC OF 40% OR LESS DIGESTION GETS COMPLETED IFSUFFICIENT TIME IS PROVIDED.

    BIOGAS AND METHANE PRODUCTION AND AMOUNT OF SUBSTRATE

    DEGRADED REMAINS SAME.

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    ANAEROBIC CONTACT DIGESTER

    BIOMASS SETTLED IN A SECOND TANK, RECYCLED TO THEDIGESTER.

    RECYCLE GIVES HIGHER SRT AND EFFICIENCY MIXING IN THE FIRST TANK AND EFFICIENCY OF SETTLING IN

    THE SECOND TANK IMPROVES PERFORMANCE.

    REQUIRE HRT OF 10 DAYS OR MORE.5. Bio-degradation of xenobiotic Compounds

    Man made compounds that are detected in the environment in unusually high

    concentrations are called xenobiotic. This term is also applied to those compounds thatoccur naturally, but due to mans activities, are deposited in the environment inunnaturally high concentrations Such xenobotic compounds are not readily biodegradable

    since their molecular structures or bond sequences are not readily recognized by existing

    degrading enzymes.Types of compounds: Aliphatic halo-hydrocarbons, cyclic halocarbons, aromatic

    halocarbons, polychlorinated biphenyls, synthetic polymers, alkyl-benzyl sulphonate.

    These are examples of xenobotic compounds released into the environment that are not

    easily degraded by microorganisms.In general, xenobiotics are either recalcitrant because they are chemically stable

    or their decomposition by catabolism leads to the production of toxic compounds.

    Modern research in microbial metabolism in mixed cultures has indicated the possibilityof biotechnology for the decomposition of many of these compounds.

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    6. Biomass For Energy

    Renewable Energy potential and achievements for India

    Source Approximate

    Potential

    Status

    (as on 31 MARCH1998)

    Biogas plants 12 million 2.71 million

    Improved wood

    Stoves

    120 million 28.49 million

    Biomass power

    and gasifiers

    1700 MW 29.5 MW

    Biomass based cogeneration 3500 MW 84 MW

    Solar photovoltaic 20 MW/km

    2

    32 MWSolar water heating systems 35 MW/km2

    13.3 MW

    Wind power 20,000 MW 970 MWSmall hydro power

    (up to 16 megawatt)

    10,000 MW 155.38 MW

    Refer: TERI -ENERGY DATA DIRECTORY AND YEAR BOOK,

    1998, p 470

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    7 Agriculture & Biotechnology: Environmental Benefits

    BIOFERTILIZER

    o Microbial inoculants, carrier based preparations containing beneficialmicrobes in a viable state intended for seed or soil application.

    o Improve soil fertility and help plant growth.o In the root environment, number and biological activity of the desired

    microbes are increased which produces plant nutrient nitrogen.

    Biofertilizers are microbial inoculant cultures of agronomic value, in nitrogen

    fixation, phosphate solubilization and release of plant growth regulators.

    In 1930s, lab preparations of RHIZOBIAL inoculants also known as LEGUMEinoculants were tested. Later these became industrial products in U.S., Europe, Australia

    and India. Rhizobia are most effective in converting atmospheric nitrogen to ammonia in

    symbiosis with legumes.SPIC Science Foundation has successfully developed technology to produce efficientinoculants of rhizobia for soyabean, chickpea, groundnut, black gram and other pulses.

    The carrier material ands nutrient formulation ensures extended shelf life of the microbial

    inoculants. The Foundation has also developed specific Azospirillum strains ensuringenhanced productivity.

    Azospirilla enhance root biomass and fix nitrogen in associative symbiosis with

    cereals, sugar cane and cotton. Carrier based AZOSPIRILLUM and AZOTOBACTERinoculants for non-leguminous crops have become popular in India, in recent times.

    Azotobacter inoculants promote seed germination and initial vigour of plants due to

    growth substances produced by the organism.

    BLUE GREEN ALGAE (cyanobacteria) play a role in the nitrogen economy oftropical rice soils. They can be cultured in open-air tanks and used for rice cultivation.

    Algal inoculation of rice fields in India has shown their use as biofertilizer.

    ANABAENA, NOSTOC and TOLYPOTHRIX are free-living blue green algae that fixnitrogen under rice cultivation.

    Azolla-anabaena symbionts generate about 40 kg N/ ha along with addition of bulk

    quantities of organic matter of azolla biomass.

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    BACILLUS MEGATERIUM VAR PHOSPHATICUM cells have capacity to

    convert rock phosphate to soluble forms useful to plants.

    Sekhar Nautiyal, at National Botanical Research Institute, Lucknow, identified

    bacteria, PSEUDOMONAS FLUORESCENS, which increased crop productivity by 20%

    in some crops and produced plant growth hormones. It also helped to make phosphatemore soluble in water. (Business world, p104.Sept.7, 1997)

    VAM fungi: [Vesicular Arbuscular Mycorrhiza]

    Reference Books:

    1. Biofertilizers in Agriculture and Forestry by N.S.Subba Rao, Oxford and IBH Publ. Co

    New Delhi, (1993)

    2. Bio-fertilizers: Instruction cum- Practical Manual for IX and X classes, Rs.25/=N C E R T book: Available from:Business Manager, RPDC, NCERT,

    108, 100ft Road, Hasker Halli Extn,

    Banashankari, 3rd

    Stage,Bangalore.560085

    Phone: 6725740

    Reference: (General Article): Perspectives of soil fertility management with a focus onfertilizer use for crop productivity Sankaram Ayala and E. V. S. Prakasa Rao

    Current Science, Vol. 82, No7, 10, April 2002, pp 797 807.

    BOOK: Biotechnology of Bio-fertilizers, Editor: S.Kannaiyan, Narosa PublishingHouse, New

    Delhi, 2002 [660.63 95328]

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    Bioremediation

    WHAT IS BIOREMEDIATION?

    Remediation is a process to remove

    contaminants such as gasoline, kerosene and

    fuel oil from soil. Bioremediation uses

    naturally occurring microorganisms such asbacteria, fungi, or yeast to decompose

    harmful chemicals into less toxic or nontoxic

    compounds.

    Microorganisms, like all living organisms,need nutrients (such as nitrogen, phosphate

    and trace metals), carbon and energy to

    survive. Microorganisms break down a wide

    variety of organic (carbon-containing)compounds found in nature for energy for

    their growth. Many species of soil bacteria,

    for example, use petroleum hydrocarbons asa food and energy source. This natural

    process transforms the petroleum

    hydrocarbons into harmless substancesconsisting mainly of carbon dioxide, water

    and fatty acids.

    WHY BIOREMEDIATION?

    In Ohio, over 15,000 underground storagetanks that store petroleum, heating oil and

    other materials are leaking. Oil spills and

    leaks at industrial sites, feed lots and railyards have resulted in hundreds of tons of

    petroleum contaminated soil (PCS)

    throughout the state.

    Petroleum contaminated soil, unregulated

    and left to evaporate into the atmosphere,can release potentially harmful volatile

    organic compounds into the atmosphere.

    Petroleum products can seep into soil andcontaminate underlying ground water.

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    Runoff from unregulated sites can carry

    petroleum contaminants off-site into nearbywaterways.

    Ohio EPA considers bioremediation

    technology to be one safe solution to thePCS problem. Instead of transferring

    contaminants from one environmental areato another (for example, from water to the air

    or to land), bioremediation decomposes

    petroleum products. Ohio EPA has strictemission standards to ensure permitted

    facilities don't negatively impact human

    health or the environment.

    WHAT ARE THE ALTERNATIVES TO

    BIOREMEDIATION?

    While there are several treatment methods,

    there is no single technology that can beapplied to every PCS site. The remediation

    method applied should be most appropriate

    for specific site characteristics. All of thefollowing treatment methods are better

    options than land filling or leaving the soil

    on the ground.

    Land farming: This method involvesspreading soil over an open area, allowingcontaminants to be released into the air. If

    not properly contained, rainwater runoff

    could cause PCS to be carried off-site.

    Soil vapor extraction: This method

    involves venting air in the soil to removevapors which may be controlled or vented to

    the air. As soil is cleaned to acceptable

    levels, it normally remains in the ground atthe original location. This process releases

    contaminants into the air.

    Thermal treatment: Also referred to as

    thermal desorption, this method involves

    heating soil to 250-700 degrees Fahrenheit.Thermal treatment differs from incineration

    because there is no combustion of the soil.

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    Contaminants are separated from the soil and

    released into air pollution control equipmentto minimize emissions being released into

    the air.

    CAN BIOREMEDIATION FACILITIESACCEPT HAZARDOUS SUBSTANCES?

    Bioremediation facilities are NOT permitted

    to accept petroleum contaminated soilscontaining hazardous substances, including

    PCBs and pesticides. Bioremediation

    facilities are NOT permitted to accept soils

    containing hazardous waste.

    Petroleum contaminated soil is not

    hazardous waste. Petroleum contaminatedsoil is not hazardous to touch or handle.

    Lignite and Biotechnology

    LocationNeyveli, India

    Responsible

    organization(s)Neyveli Lignite Corporation (NLC) within the Ministry

    of Coal. Situated in the south of India in Tamil Nedu,NLC mines 11MT of lignite annually, of which 9.5 MT

    are used for electricity generation. Industrial aspect of

    activities is supported by the United Nations Industrial

    Development Organization (UNIDO).

    Description The project was initiated to facilitate the establishmentof a Lignite Fuel and Energy Research Institute (LERI)

    at NLC in order to ensure that Indian lignite is utilised

    to its full potential and that environmental problemstemming from its use are minimised. One aspect of the

    project is to assist NLC with the industrial aspects of its

    work on mine spoil reclamation. The mining operationsat NLC covered a huge area by mine spoil, and the

    problem continues to grow. NLC explored ways to

    overcome this concern.

    The main problem is that the mine spoil is devoid of

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    both humic substances and micro-organisms and hence

    unable to support crop growth. NLC undertook tests to

    enrich the sterile mine spoils with additives in order to

    transform the spoils into a suitable substrate for plantgrowth. The tested additives include biofertilisers,

    humic acid, other organic substances and inorganicfertilizers. These were tested in various combinations ina large variety of plant species including maize, millet,

    rice, sugarcane, fruit trees and flowers. The biofertiliser

    and the humic acid supplements tested are beingproduced on the pilot scale by NLC, and lignite itself is

    one of the raw materials in the production process. The

    biofertiliser is produced by growing five strains of

    micro-organisms in fomenters. These are harvested andadsorbed onto lignite to produce a jelly-like substance

    which is the initial product. The humic acid is currently

    produced from lignite by digestion with potassiumhydroxide. This produces an undesirable effluent.

    The project provides support towards the developmentof the production processes for the biofertiliser and the

    humic acid and a new biotechnology process for

    producing humic acid is being developed. In addition toreducing production costs, this will probably also

    alleviate the need for the harsh chemicals that are used

    in the present production process, and thus reduce theenvironmental impact of the process. It also provides an

    elegant solution to an environmental problem; using thematerial extracted from a mining operation, and

    biotechnology, to alleviate the environmental problemcaused by the mining. NLC is also investigating

    biotechnology solutions to other environmental

    problems. These include the biological treatment ofeffluents, including effluent from the lignite briquetting

    and coking plant to reduce phenol content, and for

    reclamation of the ash pond which now covers morethan 25 hectares. NLC is also monitoring the fate of

    residual chemicals in the environment.

    Issues

    addressedThe operations at NLC had, and continue to have,

    considerable environmental impact. The project

    supports the above biotechnology approach which maybe able to significantly reduce some of the negative

    environmental effects. Many other mining sites in India,

    and many other developing countries with miningoperations, face similar environmental problems. There

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    is thus considerable potential for applying the products

    and technologies developed at NLC at other sites both

    within and outside of India.

    Objectives To assist NLC to develop technologies which will

    mitigate the environmental consequences of past andpresent mining and related activities, and which may be

    replicable at other sites.

    Results

    achievedTechnologies have been developed which can

    effectively reduce the environment impact of miningactivities.

    Lessons

    learnedEnvironmental protection technologies are not always

    restrictive to industry nor are they necessarily

    expensive.

    Financing The Government of the Federal Republic of Germany

    through the Industrial Development Fund.

    Contact Mr. Grant Ramsay, Chemical Industries Branch,UNIDO Vienna International Centre

    P.O. Box 400 A-1400 Vienna Austria

    Telephone: (+43 1) 21131 - 3774

    National Environmental Engineering Research Institute

    National Environmental Engineering Research Institute, Nagpur, pursues

    an effective R & D programme in Environmental Science and Technology

    to enable solutions to backlog and future environmental problemsemanating from developmental imperatives in various socio-economic

    sectors. The institute while fulfilling its commitment towards the nationaland social missions and CSIR thrust area activities, has made a significantcontribution in the recent past, in the areas of institute's R & D, viz.

    Environmental Monitoring; Environmental Biotechnology, Hazardous

    Waste Management; Environmental System Design, Modelling andOptimization; Environmental Impact and Risk Assessment; andEnvironmental Policy Analysis.

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    The institute has retained its repute in International Scientific

    Collaboration by undertaking joint R&D ventures with United NationsEnvironmental Program (UNEP), World Health Organization (WHO), and

    Danish International Development Agency (DANIDA). The institute is

    designated as WHO Collaborating Centre on Environmental Health.

    Bioinformatics Centre at NEERI aims to serve as a National Information

    Resource Centre on Environmental Biotechnology by providing totalcomputer support in the form of information, data processing & analysis in

    Biotechnology in general and Environmental Biotechnology in particular.

    The need to keep abreast of the latest information on advances anddevelopments in environmental biotechnology has become imperative for

    rapid progress in research, production and application. Accordingly, to

    cater the needs of the scientific community of the host organization andthe local needs, an Environmental Biotechnology Bibliographic Database

    (EBBD), has been developed covering the literature from a large number

    of Biotechnology journals available in NEERI Library. EBBD is abibliographic database developed initially on CDS/ISIS and converted to

    dBASE IV by a conversion program. Apart from a logical query facility, aretrieval facility on author and keywords is also available for the database.

    A database including the data entry , data handling and query software,

    named QABIS has been developed for ongoing and closed Biotechnology

    projects in NEERI. The query facility for QABIS is available on Lab code,Scientist name and area code. The software is written in dBASE. The

    centre has collected the data on biotechnology equipments from 59

    biotechnology research organizations from India and a directory databaseon equipment details has been developed with a query software to retrieve

    the information on Equipment, Lab name, and city. The database contains

    information on 400 equipment details, contact persons and the accessfacilities. HCIS ( Hazardous Chemical Information System) is a software

    Package developed at the centre using 'C' programming language for quick

    retrieval of information about 276 hazardous chemicals. The user interface

    has been designed with a view to enhance the utility of the informationreadily available as ASCII text files which is maintained by USEPA under

    Integrated Risk Information System(IRIS). The searches are based on

    different chemical names. A directory database on thesis submitted toNagpur University in Life Sciences and Biotechnology is in progress and

    the database comprises of 225 thesis submitted during 1987 to 1996.

    CD-ROM Databases viz. Ei-Energy and Environment; CCINFO Disc

    Series, and AHEAD series have been purchased for the centre and

    information retrieval services are provided on demand to the clientele ofthe host institute and other organizations.