INDONESIAN SCHOLARS INTERNATIONAL CONVENTION (ISIC) 201515th CONFERENCE - TIMII (TEMU ILMIAH MAHASISWA INTERNATIONAL INDONESIA)

London, 3 – 4 October 2015

-EXTENDED ABSTRACT-

MASTIMEDIS “MASTITIS ELECTRICAL BIOMEDIS”MASTITIS THERAPY EQUIPMENT INNOVATION IN DAIRY CATTLE

Bekti Sri Utami*, Rifai, Handriawan Junianto. Brawijaya University, Indonesia

*bektisriutami@gmail.com

BACKGROUNDDomestic Fresh Milk Productions (SSDN) are still far from the minimum requirement to meet the domestic

market. In the year of 2007, SSDN has covered only 22,9 % of domestic milk consumption. The level of milk consumption in Indonesia per capita recorded in 2007 has reached 10,47 kg/capita/year. Based on Ministry of Industry (2008), domestic milk consumption are still below compared to ASEAN countries, Philippines with 20 kg/capita/year, Malaysia with 20 kg kg/capita/ year, Thailand 20-25 kg/capita/year and Singapore with 32 kg/capita/year.

Coupled with the presence of mastitis disease that can degrade the quality of milk. Mastitis or inflammatory disease of the udder is still the main problem in dairy cattle as disadvantage which decreases the milk production and the quality of milk production, the elimination of milk to be consumed as first choice, the cost of care and medical treatment which leads to cow being of no use. There are two types of mastitis, clinical mastitis and subclinical mastitis. Refer to Khadija (2006), subclinical mastitis are commonly detected as Staphylococcus aureusand Streptococcus agalactiae. Recorded by several intensive research in dairy cattle throughout West Java, Central Java and East java, generally subclinical mastitis are ranged between 37 to 67 % and clinical mastitis between 5 – 30 % (Abrar, 2002).

Staphylococcus aureus and Streptococcus agalactiae are considered to be important mastitis bacteria in dairy cattle due to its microorganism that lies in the ill and healthy udder. Moreover, it is more difficult to deal with the infected bacteria as most of it are resistant to any kind of antibiotics. Besides, the use of antibiotics leads to another problem, the presence of antibiotic residue in the milk or on its form of ready products.

METHODOLOGYThe applied of electrode pen is appropriating to where the teat and mamae gland of the udder that being

affected. The area of Staphylococus aureus bacteria are given with electric current of 9 volt in 5 minutes with direct current frequency of 900 hertz (Firmansyah, 2013). This kind of process is actually the way to inhibit the development process of bacteria and break it down.

Magnetic field is fueled by power supply of 9 colt from the input alternating electric current of AC 220 volt which is already being converted to direct electric current (DC) (Haltiwanger, 2010). The electric current from power supply are being controlled by arduino uno to give frequency requirement. The electric current from electrode will damage the bacteria by perforating the cell membrane.

Image. 1 Principle of Electroporation of MASTIMEDIS (Palti, 2004)

INDONESIAN SCHOLARS INTERNATIONAL CONVENTION (ISIC) 201515th CONFERENCE - TIMII (TEMU ILMIAH MAHASISWA INTERNATIONAL INDONESIA)

London, 3 – 4 October 2015

-EXTENDED ABSTRACT-

Membrane are being damaged, bacteria are dead caused by electric current. Electric current are inhibiting the process of mitochondria-organelle to produce energy inside the bacteria body. The function of bacteria physiology working through electrochemical is also being inferred due to its barriers in splitting themselves with mitosis way from electric current. The exposure of electric field of 900 hertz frequency on 9 volt current in 5 – 10 minutes of self-cleavage will be paralyzed and destroyed. Therefore, mastitis can be cured faster due to pathogen bacteria that is being destroyed by electric current mentioned above.

RESULTS/DISCUSSIONStaphylococcus aureus and Streptococcus agalactiae are positive gram bacteria that are resistant to methicillin and amoxicillin antibiotics. This kind of bacteria are known to be the main pathogen to cause the inflammation of the udder which leads to the mastitis to be affected.

The electric current of MASTIMEDIS can interfere the bacteria cell process to split themselves with in mitosis way. As it is known that bacteria can be affected by electric field, therefore bacteria can be damaged by the principle of Electroporation, perforating the cell membrane of bacteria using electric current (Haltiwanger, 2010). Sobrino (2006) states that electroporation is one of the principle to kill bacteria by perforating the cell membrane of the bacteria with electric current so that bacteria become unstable and leads to electrochemical compression. Qingke (2006) adds that explanation of small electric current through two electrode with direct current, 9 volt and a frequency of 900 hertz in a 5 minutes will gives a damage the bacteria cell membrane, Staphylococcus aureus or lysis of bactreria and died to it. On the other hand, the electric current does not enough to break down the cell of dairy cattle and to harm biologically due to its differences in conductivity of bacteria cell and dairy cattle.

REFERENCESAbrar, Mahdi. Dkk 2012. Isolasi Dan Karakterisasi Hemaglutinin Staphylococcus aureus Penyebab Mastitis

Subklinis Pada Sapi Perah. Jurnal kedokteran hewan Vol 6 : 1.Firmansyah, Diki. 2013. Pengaruh Tingkat Mastitis Subklinis Terhadap Kualitas Susu Sapi Perah Pfh (Peranakan

Friesian Holstein) Pada Berbagai Bulan Laktasi. Program kedokteran hewan Universitas Brawijaya , Malang.

Haltiwanger, Steve. 2010. The Electrical Properties of Cancer Cells. (Online: http://www.royalrife.com/haltiwanger1.pdf )

Khodijah, b. J. Tuasikal2, i. Sugoro2 dan Yusneti. 2006. Pertumbuhan Streptococcus agalactiae sebagai Bakteri penyebab mastitis subklinis Pada sapi perah. Seminar Nasional Teknologi Peternakan dan Veteriner. Indonesia

Palti, Yoram et al. 2004. Disruption of cancer cell replication by alternating electric fields.Qingke, L., Z. Changli, F. Junlong. 2003. Antiseptic Research of Liquid Food Under High Voltage Pulse. The

Agriculture Mechanization Research: 100-101Sobrino, López, A., R, Raybaudi-Massilia, and O. Martín Belloso. 2006. High Intensity Pulsed Electric Field

Variables Affecting Staphylococcus aureusInoculated in Milk. Journal of Dairy Science 89: 3739-3748. Department of Food Technology, University of Lleida.