The Effects of Iron (II) Sulfate on Growth and Carbon Dioxide Sequestration in Thalassiosira pseudonana and Phaeodactylum tricornutum Over the past 50 years, the Mauna Loa Observatory in Hawaii has observed increasing rates of CO 2 emmission. The CO 2 increase in 1959 was 0.95 ppm/yr, whereas in 2007 the rate of CO 2 increase was 2.14ppm/yr. (Tans, 2008) In Martin’s (1993) experiment, FeSO 4 was added to an area of the Pacific Ocean with minimal algae growth and exponential growth was observed. In this experiment algae is grown in 2-liter polyethylene terephthalate bottles to prevent contamination by trace elements in glass (Martin, 1982). Thalassiosira pseudonana and Phaeodactylum tricornutum used as model organisms for this experiment due to previous extensive research on these organisms. FeSO 4 is added to the algae medium to make the media a 1.44µM solution of iron. (Coale, 2008) Data will be collected using a PASCO GLX Explorer to measure the CO 2 concentration and pH of the media. • According to the Mauna Loa Observatory, Carbon Dioxide levels have risen from 1959 to 2007 • Changes in Carbon Dioxide are synchronized with the changes in Temperature (Hied, 1996) • Chlorosis was a disorder developed when plants grow with an iron deficiency (Stockhardt, 1882) • Iron Experiments were ran in HNLC regions (High Nutrients, Low Chlorophyll) • SoFEX was an experiment which use the appropriate number of iron by using 1 nanomol per liter in a 15 x 15 kilometer square region (Coale, 2002) The purpose of this experiment is to examine the effects that iron fertilization has upon the photosynthetic methods of different types of phytoplankton 1(a) Growth of Phaeodactylum tricornutum will be significantly different when FeSO 4 is added 1(n) There will be no significant difference in growth of Phaeodactylum tricornutum when FeSO 4 is added 2(a) Growth of Thalassiosira pseudonana will be significantly different when FeSO 4 is added 2 (n) There will be no significant difference in growth of Thasassiosiria pseudonana when FeSO 4 is added

According to the Mauna Loa Observatory, Carbon Dioxide levels have risen from 1959 to 2007

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The Effects of Iron (II) Sulfate on Growth and Carbon Dioxide Sequestration in Thalassiosira pseudonana and Phaeodactylum tricornutum - PowerPoint PPT Presentation

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Page 1: According to the Mauna Loa Observatory, Carbon Dioxide levels have risen from 1959 to 2007

The Effects of Iron (II) Sulfate on Growth and Carbon Dioxide Sequestration in Thalassiosira pseudonana and Phaeodactylum tricornutum

Over the past 50 years, the Mauna Loa Observatory in Hawaii has observed increasing rates of CO2 emmission. The CO2 increase in 1959 was 0.95 ppm/yr, whereas in 2007 the rate of CO2 increase was 2.14ppm/yr. (Tans, 2008) In Martin’s (1993) experiment, FeSO4 was added to an area of the Pacific Ocean with minimal algae growth and exponential growth was observed. In this experiment algae is grown in 2-liter polyethylene terephthalate bottles to prevent contamination by trace elements in glass (Martin, 1982). Thalassiosira pseudonana and Phaeodactylum tricornutum used as model organisms for this experiment due to previous extensive research on these organisms. FeSO4 is added to the algae medium to make the media a 1.44µM solution of iron. (Coale, 2008) Data will be collected using a PASCO GLX Explorer to measure the CO2 concentration and pH of the media.

• According to the Mauna Loa Observatory, Carbon Dioxide levels have risen from 1959 to 2007• Changes in Carbon Dioxide are synchronized with the changes in Temperature (Hied, 1996)

• Chlorosis was a disorder developed when plants grow with an iron deficiency (Stockhardt, 1882)

• Iron Experiments were ran in HNLC regions (High Nutrients, Low Chlorophyll)• SoFEX was an experiment which use the appropriate number of iron by using 1 nanomol per liter in a 15 x 15 kilometer square region (Coale, 2002)

The purpose of this experiment is to examine the effects that iron fertilization has upon the photosynthetic methods of different types of

phytoplankton

1(a) Growth of Phaeodactylum tricornutum will be significantly different when FeSO4 is added

1(n) There will be no significant difference in growth of Phaeodactylum tricornutum when FeSO4 is added

2(a) Growth of Thalassiosira pseudonana will be significantly different when FeSO4 is added

2 (n) There will be no significant difference in growth of Thasassiosiria pseudonana when FeSO4 is added