2% SeO32-

98% C-Se-C

A

C

D

74% C-Se-C

20%Se0 6%

SeO32-

G

22% Se0

19%SeO3

2-

59% C-Se-CF

Figure 6. (A) Photograph of an A. bisulcatus seed. (B) µXRF map showing distribution of Se (red), Zn (green) and Ca (blue) in the seed. (C) Se composition in the embryo within the seed (white circles in B) as determined by XANES. (D) Photograph of fungal endophyte emerging from germinating A. bisulcatus seed. (E) µXRF map showing distribution of Se (red) and Ca (green) in endophyte-containing A. bisulcatus seed and in the mycelium of the emerging endophytic fungus (inset). (F) Se composition in fungal mycelium growing out from the seed (white circles in E), and (G) Se composition in the endophyte-containing seed (black circle in E) as determined by XANES.

oo

oo

Se

E

Se

o

ooo

o

SeCa

1 mm

Se Zn Ca

B Se

o

o

0.5 mm

B