Presentation 2015 eng s

Biostimulants application on cereals, vegetables

and other crops

Dr. Serhiy Ponomarenko

Interdepartmental Science & Technology Center

“AGROBIOTECH” NAS and MES of Ukraine, Kyiv

February, 2015

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Interdepartmental Science & Technology Center "Agrobiotech“ NAS and MES of Ukraine was founded in 2000 for the purpose of

strengthening of state regulation in the field of development of ecologically safe plant growth regulators, acceleration their

introduction in agriculture of Ukraine and export abroad.

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BIOSTIMULANTS OF NATURAL ORIGIN

BIOLAN, EMISTIM C, REGOPLANT:

WIDE SPECTRUM OF USING ON 25 AGRICULTURAL CROPS

Biotechnology of micromyces cultivation from root system of ginseng

5 patents

Ginseng rootMicromyces

BRIEF TECHNOLOGY DESCRIPTION

Scientific plant

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Streptomyces avermitiles

“Agrobiotech” produces new biostimulants with bioprotective effects

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16.11.2012

First step in plant biotechnologyFirst step in plant biotechnology

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Our company “Agrobiotech” is able to produce Our company “Agrobiotech” is able to produce biostimulants in large volumesbiostimulants in large volumes. . For each month we can For each month we can help for using our technologies up to 1 000 000 hectars.help for using our technologies up to 1 000 000 hectars.

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NE ISTC Agrobiotech has license of the Ministry of Industrial Policy of Ukraine for manufacture of 15 biostimulants (certified as PGRs).

Products are protected by 32 patents of Ukraine, 4 patents of the Russian Federation.

They are certified for use in:

Ukraine (15)

Germany (1)

Belarus (4+2 in 2014)

Kazakhstan (4)

China (1+1 in 2015)

Russia (2)

Canada (Biolan in 2014)

Certification and ProtectionCertification and Protection

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Advantages: on mining of Chinese farmers our PGRs are working as “Tears of Angels” – in such small quantities and with high efficacy.

PGRs are characterized by high efficiency, low doses of using (20-30 ml per 1 ton of seed or 1 hectare of crops) and ecological safety (1 m2 gets 1*10-9 g of active substance). It is nanobio-ecotechnology of XXI centure.

The technologies of their application coincide with

existing agromethods.

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Organic certification issued by the “Bureau of international certification SIC 01-10. Regulations of organic production”

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ACHIEVEMENTS OF NOBEL LAUREATES ARE ACHIEVEMENTS OF NOBEL LAUREATES ARE

IMPLEMENTED IN UKRAINE (2013-2014)IMPLEMENTED IN UKRAINE (2013-2014)

The Nobel Prize in Physiology or Medicine 2006 was awarded jointly to

Andrew Z. Fire and Craig C. Mello "for their discovery of RNA interference -

gene silencing by double-stranded RNA" .

Andrew Fire Craig Mello

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ACHIEVEMENTS OF NOBEL LAUREATES ARE ACHIEVEMENTS OF NOBEL LAUREATES ARE IMPLEMENTED IN UKRAINE (2013-2014)IMPLEMENTED IN UKRAINE (2013-2014)

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The mechanism of the physiological

action at the cellular level

1. 1. Over the past 15 years, much Over the past 15 years, much attention is paid to the isolation of attention is paid to the isolation of eukaryotic from cells and eukaryotic from cells and determination of the biological role of determination of the biological role of small regulatory RNAs (small small regulatory RNAs (small regulatory RNA) in RNAi (RNA regulatory RNA) in RNAi (RNA interference), which is called interference), which is called posttranscriptional gene silencing posttranscriptional gene silencing

(PTGS) in plants, animals and fungi(PTGS) in plants, animals and fungi..

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2. Gene silencing is a process which results in either degradation or blocking of the translation of mRNA target molecules, it is of great importance in the adaptive virus resistance in protecting of the genome against transpositions of mobile DNA - elements and ontogenetic regulation of gene expression.

2 types of small regulatory RNA – miRNA (microRNA) and siRNA (short interfering RNA) have a dominant role in silencing.

The mechanism of the physiological

action at the cellular level

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The mechanism of the physiological action at the cellular level

3. Si/miRNA are similar in structure and function (characterized by antisense structure complementary to mRNA) and play a dual role in plants: 1) jointly with site - specific multisubunit endo- and exonucleases, which are components of RISC complex (RNA - induced silencing complex), si/mi RNA determined the life time of each mRNA molecules. They primarily destroy by degradation (cleavage) or blocking (silencing) of translation the aberrant and imperfect on the structure molecules mRNA, which may appear incorrectly in cells, 2) serve protective (antipatogenic and antiparasitic) function.

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The mechanism of the physiological action at the cellular level

4. In both cases, these biological effects are achieved by binding si/mi RNA with complementary polynucleotide link of mRNA of own cells, or pathogenic viruses, or harmful organisms such as nematodes.

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The mechanism of the physiological action at the cellular level

5. Scientists propose two

approaches to increase the number of si/mi RNA in response to pathogenesis:

1) by introducing into cells of additional number of gene copies of si/mi RNA by genetic transformation, and

2) activation of expression of own cellular genes of si/mi RNA synthesis by some specific inducers.

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MicroRNAs as regulators in plant metal toxicity response Ana B. Mendoza-Soto, Federico Sánchez and Georgina Hernández

Schematic representation of the mode of

action of miR319, miR390, miR393, and

miR398 in response to metaltoxicity.

(A) Regulation in leaves.

(B) Regulation in roots. miRNAs are

in rectangles while their targets are in

diamonds. Arrows indicate up-regulation

and blunted lines indicated own

regulation.

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The mechanism of the physiological action at the cellular level

The ecological and economic effectiveness of joint use of plant growth regulators with modern herbicides was shown for the reduction of pesticides rates.

6. During 12 years the Uman State Agrarian University led by prof. Hrytsayenko Z.M. has carried out research "Development of eco-friendly production technologies with a minimal pesticide load in crop rotations."

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Model of CO2 transfer from air into chloroplast and into carbohydrates

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Biolan on winter wheat (Uman, 2011)

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Bayer CropScience, Germany (Grodil Maxi OD, Puma Super)

DuPont International Operations Sarl, Switzerland (Harmony 75, Basis 75, Calibre 75, Granstar 75)

Nufarm GmbH & Co KG , Austria (Dicopur F600)

Dow AgroSciences GmbH , Austria (Prima, Esteron 600, Lontrel 300, Lancelot 450 WG)

Syngenta International AG, Switzerland (Lintur 70WG, Dual Gold 960, Fusilade Forte 150)

Herbicides of 14 leading world companies (apart and jointly with bioregulators) were used in 12-years research work of Biology Department of the Uman State Agrarian Institute of Horticulture:

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Effect of different rates of the herbicide Caliber and Biolan on the weed infestation of winter wheat crops, 2012

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Biological features of herbicide Prima (Dow AgroSciences GmbH , Austria) application separately and jointly with Biolan, impact on the productivity of winter triticale (2005-2007, Forest-steppe, Ukraine.

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21

75

29

82

28

29

24

36 25

76

23

45

30

51

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14

29

01

2000

2200

2400

2600

2800

3000

3200

1 2 3 4 5 6 7 8 9

Bacteria, HIP05 = 61-70

Explanatory notes1. No treatment2. No treatment + hand

weedings3. Emistim C 10 ml/ha4. Lintur 70 WG 120 g/ha

(67% of the norm)5. Lintur 70 WG 150 g/ha

(83 % of the norm)6. Lintur 70 WG 180 g/ha (the

norm)7. Lintur 70 WG 120 g/ha +

Emistim C 10 ml8. Lintur 70 WG 150 g/ha +

Emistim C 10 ml9. Lintur 70 WG 180 g/ha +

Emistim C 10 ml

The results of experiments during 3 years.The complex of soil bacteria population was increased by 40%

The total quantity of bacteria in the rhizosphere of spring wheat plants after 25 days after herbicide Lintur 70 WG (Syngenta) and bioregulator Emistim C application. Uman National University of Horticulture

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The total quantity of Micromycetes in spring wheat plant rhizosphere after 25 days after herbicide Lintur 70 WG and bioregulator Emistim C application. Uman National University of Horticulture

25

5

32

6

30

4

26

9

28

7

26

6

33

6

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1

29

7

250

270

290

310

330

350

1 2 3 4 5 6 7 8 9

Micromycetes, HIP05 = 12-24

The results of experiments during 3 years.The complex of micromecete complex was increased by 32%

Explanatory notes1. No treatment2. No treatment + hand

weedings3. Emistim C 10 ml/ha4. Lintur 70 WG 120 g/ha

(67% of the norm)5. Lintur 70 WG 150 g/ha

(83 % of the norm)6. Lintur 70 WG 180 g/ha (the

norm)7. Lintur 70 WG 120 g/ha +

Emistim C 10 ml8. Lintur 70 WG 150 g/ha +

Emistim C 10 ml9. Lintur 70 WG 180 g/ha +

Emistim C 10 ml

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Effect of biostimulants on germination, yield and the rate of plant infestation.

Winter wheat Oksana on the infected background, Institute of Breeding (Odesa), 2010-2013

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Biostimulants` efficiency in comparison with traditional chemical products – seed protectants against wheat nematode, ground beetle, turnip moth, Oscinella (2011)

1 – amount of infected caryopsides;2 – biological efficiency %;

*- Yunta Quadro – fungicide “Bayer Crop Science”** - Selest Top – insecticide “Syngenta”*** - Alpha-cypermethrin – insecticide “Bayer Crop

Science”

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Efficiency of new biostimulants in comparison

with other products – seed protectants against

agents of mold and blight of soybean (2011)

1 – amount of infected caryopsides;2 – biological efficiency %;

* – Lamardor – fungicide “Bayer Crop Science”;** – Yunta quattro – fungicide “Bayer Crop Science”;*** – fungi Mucor spp., Rhizopus spp., Aspergillus spp., Penicilium spp., Tricholhecium

roseum.

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Efficiency of biostimulants in comparison with

other products – seed protectants against agents

of mold and blight of corn (2011)

1 – amount of infected caryopsides;2 – biological efficiency %;

* – Lamardor – fungicide “Bayer Crop Science”;** – Yunta quattro – fungicide “Bayer Crop Science”;*** – fungi Mucor spp., Rhizopus spp., Aspergillus spp., Penicilium spp., Tricholhecium

roseum.

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It is proved that these growth inducers have unique complex

of properties: promote the harvest and its quality,

release genetic potential of variety.

Moreover, grain got in variants with application

of growth inducers , does not contain mycotoxins

and residual quantities of pesticides and

pretend to ecologically safe product.

Results for Yield of Back-to-back testing (Odessa, 2011)

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Influence of PGRs in compositions with bacterial products on winter wheat productivity. Chernihiv

Institute APP

37,0

40,0

38,637,8

43,644,5

30,0

32,0

34,0

36,0

38,0

40,0

42,0

44,0

46,0

1 2 3 4 5 6

Yield

Variant, % to Control:

1. Control

2. Biolan, 25 ml/t + 8,1%

3. Diasophyt, 400 ml/t + 4,3%

4. Polymixobacterin +2,1%

5. Biolan + diasophyt + 17,8%

6. Biolan + polymixobacterin + 20,2%

Bioregulators of the ISTC "Agrobiotech" enhance the development of microflora as against a pesticide action. This is shown when used jointly Biolan with Polymixobacterin and Diasophyt

LSD 1,5 c/ha

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Biostimulant Biolan is new

biological fertilizer?!

The diagram of results of Biolan’s use, its efficiency at various levels of a mineral food in winter wheat cultivation (2008-2013)

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Soil Improvement under Biolan action

ControlOGIs

Germany

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Nematode resistance on sugar beetwith Regoplant

With biostimulants Without biostimulants

Control group seeds germinated but perished on the 5th day, while group with biostimulants dynamically developed.

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Biostimulants` Test for Sugar beet:Yield and Nematicidic Efficiency*

*Tests in the Institute of Sugar Beet, Ukraine, 2010**Number of eggs and larvae per 100 cm3 of soil

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Regoplant's influence on vegetable productivity and

qualityPlace of the tests: Scientific-research farm “Velykosnitynske”, Kyiv region. 2012

1. Increase of leaf surface by 15-47 cm2/plant.

2. Increased plant resistance to diseases and pests

(aphids, Colorado beetle, powdery mildew, cercosporosis, Phytophtora infestans, Alternaria blight.

P-490

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Note: * - dry matter (soluble).

** - Total sugars, g/dm3

1. Increase of leaf surface by 15-47 cm2/plant.

2. Increased plant resistance to diseases and pests

(aphids, Colorado beetle, powdery mildew, cercosporosis, Phytophtora infestans, Alternaria blight.

Stimpo's influence on vegetable productivity and qualityPlace of the tests: Scientific-research farm “Velykosnitynske”, Kyiv region. 2012

P-490

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Regoplant influence on the productivity and quality of vegetables

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29

18

21,2

29

35,2

20

25,327,3

34,4

0

10

20

30

cucumber tomato red beet carrot whitecabbage

Yield, t/ha, Control Regoplant 50 ml/ha

Increase, %

Cucumber +26,6%

Tomato +17,7%

Red beet +21,3%

Carrot +26,5%

Cabbage +26,0%

* the quantity of diseases and pests reduced by 54%** reduced nitrates content*** increased content of sugars and vitamins

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Stimpo's and Regoplant's influence on strawberry productivity and quality. 2012

P-490

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Influence of Regoplant on yield and quality of

grapes. NSC VE Tairova, 2013-2014

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Potato (Sluch and Kimeria) yield after use of Cruiser 350 g/l (Syngenta, Switzerland) and reduced by 20 and 40% doses with biostimulants Biolan, Stimpo and Regoplant. 2013-2014

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Cost-effectiveness of bioregulators on potato Treasury crops in Euro

This technology allowed a 60% reduction in the quantity of wireworms in the soil. Institute of Potato, 2012-2014.

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Biolan Control

Biolan on sunflowers in Drabiv fields, Ukraine

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Biostimulants during 10 years in Germany

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Example of Effectiveness

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The 1st World Congress on the use of Biostimulants in Agriculture. 2012. Strasbourg, France

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The 1st World Congress on the use of Biostimulants in Agriculture. 26-29 November, 2012. Strasbourg, France

More than 650 delegates representing 450 companies and organizations

from 55 countries have took part in the Congress.

The 2nd World Congress on te use of Biostimulants in Agriculture will be in Florence, Italy, in 16-19 Nov., 2015 www.biostimulants2015.com

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“We are confident in the imminence of the time when growth regulators

will be studied to the degree where they can be ranked as the gold

reserve of the agrochemical methods invariably increasing a harvest

yield, and can be recommended for universal application” L. A. Khristeva and coauthors, 1966

“Мы уверены в неизбежности того времени, когда регуляторы роста будут

изучены на таком уровне, что они будут названы золотым запасом агрохимических

методов, неизменно увеличивая урожайность, и могут быть рекомендованы для

универсального применения”.                                     Л. А. Христева и соавторы, 1966

Lastly

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CONTACT INFORMATION

Dr. Serhiy Ponomarenko

Phone: +(380-44) 559-66-17

E-mail: sponom@ukr.net

www.agrobiotech.com.ua

Interdepartmental Science & Technology Center

“AGROBIOTECH” NAS and MES of Ukraine, Kyiv