PROJECT PORTFOLIO

FRED MARTIN12/08/2009 Dr. GiorgiBIOSC 2

Invt4_10x_Com

positeRG

B_002

.jpg

20091207 /Nikon2 /10x ; DIC: 73ms/ 1,503/ 0.95/ 4,095 R: 97ms/ 79/ 0.63/ 4,095 G: 200ms/ 239/ 0.95/4,095 B:500ms/223/0.77/4,095

SP

EC

IME

N (A

1)

NIKON, FLUORESENCE, MOLECULAR PROBES SLIDE: TISSUE

invt4_a1_Com

posite_RG

_DapiLU

T_75.jpg

20091123 /Nikon2 /20x ; DIC: 20ms/ 79/ 0.94/ 3,310 R: 47ms/ 79/ 0.94/ 3,310 G: 41ms/ 95/ 0.67/3,054 B:392ms/159/0.51/2,830

SP

EC

IME

N (A

2)

NIKON, FLUORESENCE, MOLECULAR PROBES SLIDE: TISSUE

FluoCells prepared slide #2: BPAE bovine pulmonary artery endothelial cells

Image A

- 20091105: Zeiss #1 20x

ZEISS, FLUORESENCE, MOLECULAR PROBES SLIDE: CELLS

FluoCells® prepared slide #1: BPAEC, Zeiss 63x

20091016_INV

G1_A

RE

A3_C

ompo

siteAdj_jpg.jpg

ZEISS, FLUORESENCE, MOLECULAR PROBES SLIDE: CELLS

NIKON, FLUORESENCE, MOLECULAR PROBES SLIDE: CELLS

FM

_INV

T1_A

1_C

om

positeAdjR

GB

_xDIC

BP

AE

CE

LLS IN

VIT

RO

GE

N #1

20x_LilyAnther_F

luro_RG

B.jpg

20091123 /Nikon2 /20x ; R: 47ms/ 79/ 0.94/ 3,310 G: 41ms/ 95/ 0.67/3,054 B:392ms/159/0.51/2,830

SP

EC

IME

N (B

)

NIKON, FLUORESENCE

FM

_lycopodiumC

aptured_F

luoroRG

BnD

IC_20x_2.jpg

20091123 /Nikon2 /20x ; DIC: 20ms/ 79/ 0.94/ 3,310 R: 47ms/ 79/ 0.94/ 3,310 G: 41ms/ 95/ 0.67/3,054 B:392ms/159/0.51/2,830

SP

EC

IME

N (C

)

NIKON, FLUORESENCE + DIC

SP

EC

IME

N (F

)T

ypha_10x_Com

posite_001_RG

B_A

dj

20091207 /Nikon2 /10x ; DIC: 73ms/ 1,503/ 0.95/ 4,095 R: 97ms/ 79/ 0.63/ 4,095 G: 200ms/ 239/ 0.95/4,095 B:500ms/223/0.77/4,095

COMPOSITE CHANNEL: RED

CHANNEL: GREEN CHANNEL: BLUE

NIKON, FLUORESCENSE (3 CHANNELS + OVERLAY)

Pollen Grain: Abutilon x hybridium @ 20x

EM Channel: Green Exp: 96.7ms Gain:7.1 Offset: -876

EM Channel: Red Exp: 96.7ms Gain:7.1 Offset: -876EM Channel: (BF) Exp: 16.7ms Gain: 4.5 Offset: -161

Composite

OLYMPUS, OWN SPECIMEN, FLUORO OVERLAY + 3 CHANNELS

fm_

_pseudocolor_lycopod_20x_Com

positeRG

Bn

DIC

.jpgS

PE

CIM

EN

(D)

20091207 /Nikon2 /20x ; “R”: 13ms/ 159/ 0.51/ 2,830 “G”: 6.6ms/ 159/ 0.51/ 2,830 “B”: 12.0ms/ 159/ 0.51/ 2,830

NIKON, BRIGHTFIELD (3 CHANNEL PSEUDO-COLORIZATION)

Multichannel _S

hrim

p_Pseudocolor2_R

GB

.jpgS

PE

CIM

EN

(E)

20091118 /Nikon4 /20x ; R: --ms/ --/ --/ -- G: --ms/ --/ --/ -- --B: ms/ --/ --/ -- Note: metadata not logged

NIKON, BRIGHTFIELD (3 CHANNEL PSEUDO-COLORIZATION)

Cilia

PSCCE Cells

Basal Lamina

Pseudo Stratified Ciliated Columnar Epithelial Cross section: area 3 image

Cilia

Basal Lamina

Nucleus

Cilia

(Apparent Stratification)Exp. 500.7ms

Hist: 79/252

Gamma: 0.99

Auto_exp: OFF

Gain: 3.1x

2009.10.01Leica #2 60x (dry)

Nucleolus {

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LEICA, BRIGHTFIELD

48BitDepth:20 / 255Histogram:OnAuto_Exp?0.97Gamma:

2009-10-07DateImage:53 %Brightness:2.2 xGain:1.0 sExposure:

PSCCE CellsCell Membrane

Cilia(Apparent Stratification)

Nuclei

Basal Lamina

LEICA, BRIGHTFIELD

ZEISS, DIC

FM

_INV

T4_A

1_D

IC-0005.JP

G

20091209 /ZEISS 1 /20x ; DIC : EXP 69ms/ CT -0.50 / GAMMA 1.0 / BRT 1.0

- Interphase

- Metaphase

- Anaphase- Telophase

- Prophase

RESULTS: DIGITAL COUNT DETAIL – FIELD OF VIEW A: Onion Root Tip

Meta Data Scope:

Olympus 3 (A)

10x, rheo: full

Iris ~ 30% 1.1 ms2.8 gain-1,860 offset

Note: Manual countdone at 20x on Olympus3

OLYMPUS, MORPHOMETRY