Scanning electron microscope image of a snout beetlehttp://remf.dartmouth.edu/images/insectPart2SEM/source/20.html Image: public domain. Feb. 2012

Calculating Linear Magnification of an Electron-Micrograph using it's scale bar

There are three steps:

Measure the length of the scale bar - in mm.

Convert your measurement into µm.

Calculate the magnification.(Magnification = measured length /the number written.As long as you have the SAME UNITS)

Magnifiation = the number of times bigger the scale bar actually is, compared to the measurement written on it?

32mm

32 x 1000 = 32000µm

32000µm / 100 µm = 320x magnification

Scanning electron microscope image of a snout beetlehttp://remf.dartmouth.edu/images/insectPart2SEM/source/20.html Image: public domain. Feb. 2012

Calculating Specimen Size using a scale bar

There are four steps:

Measure the length of the Specimen in mm.(show working)

Measure the length of the scale bar in mm.

Calculate how many scale bar lengths make the specimen. (Divide length of specimen by length of scale bar)

Calculate the size. Multiply the scale bar label by the last answer.(UNITS are the same as the scale bar)

2.6 x 100 µm = 260µm

Scanning electron microscope image of a snout beetlehttp://remf.dartmouth.edu/images/insectPart2SEM/source/20.html Image: public domain. Feb. 2012

Magnifiation = x 320

Calculating Specimen Size using magnification

There are three steps:

Measure the length of the Image of the Specimen in mm.(show working)

Convert the length of the Specimen image to µm.(x 1000)

Calculate the actual size. Divide the length of the specimen image by the magnification(UNITS are µm)

Electron microscope image of pollen grains from oriental lily.http://remf.dartmouth.edu/images/botanicalPollenSEM/source/3.html Image: public domain. Feb. 2012

Calculating Linear Magnification of an Electron-Micrograph using it's scale bar

There are three steps:

Measure the length of the scale bar - in mm.

Convert your measurement into µm.

Calculate the magnification.(Magnification = measured length /the number written.As long as you have the SAME UNITS)

Magnifiation = the number of times bigger the scale bar actually is, compared to the measurement written on it?

magnification

Electron microscope image of pollen grains from oriental lily.http://remf.dartmouth.edu/images/botanicalPollenSEM/source/3.html Image: public domain. Feb. 2012

Calculating Specimen Size using a scale bar

There are four steps:

Measure the length of the Specimen in mm.(show working)

Measure the length of the scale bar in mm.

Calculate how many scale bar lengths make the specimen. (Divide length of specimen by length of scale bar)

Calculate the size. Multiply the scale bar label by the last answer.(UNITS are the same as the scale bar)

Electron microscope image of pollen grains from oriental lily.http://remf.dartmouth.edu/images/botanicalPollenSEM/source/3.html Image: public domain. Feb. 2012

Magnification = x 2500

Calculating Specimen Size using magnification

There are three steps:

Measure the length of the Image of the Specimen in mm.(show working)

Convert the length of the Specimen image to µm.(x 1000)

Calculate the actual size. Divide the length of the specimen image by the magnification(UNITS are µm)

Human leukocyte - showing golgi apparatus http://remf.dartmouth.edu/images/humanBloodCellsTEM/source/3.html Image: public domain. Feb. 2012

Calculating Linear Magnification of an Electron-Micrograph using it's scale bar

There are three steps:

Measure the length of the scale bar - in mm.

Convert your measurement into nm this time.

Calculate the magnification.(Magnification = measured length /the number written.As long as you have the SAME UNITS)

Magnifiation = the number of times bigger the scale bar actually is, compared to the measurement written on it?

magnification

Calculating Specimen Size using a scale bar

There are four steps:

Measure the length of the Specimen in mm.(show working)

Measure the length of the scale bar in mm.

Calculate how many scale bar lengths make the specimen. (Divide length of specimen by length of scale bar)

Calculate the size. Multiply the scale bar label by the last answer.(UNITS are the same as the scale bar)