1. Field sampling

2. Common garden acclimation 3. Physiology studies

Desert Cool montane Cold montane

Common garden 1

(optimum, N=315)

Animal performance

• Thermal tolerance

• Thermal preference

• Swimming

• Growth

• Behavior

3 ecotypes × 3 water temperatures (in triplicate)

Trout mechanisms: common garden research

Zhongqi Chen1,*, Brian Small1, Christopher Caudill1, Jon Masingale1, Shawn Narum2, Ronald Hardy1

1 University of Idaho; 2 Columbia River Inter-Tribal Fish Commission * zhongqic@uidaho.edu

Growth study (12-16 weeks)

• Growth performance & efficiency

Maturation and Fecundity

• Egg size & number

• Reproductive physiology

Desert Cool montane Cold montane

This project was supported by NSF award number OIA-1757324 from the NSF Idaho EPSCoR Program and by National Science Foundation. The authors thank Jeongwhui Hong, Tim Boyle, Seunghan Lee, Hossain Sakhawat and Yang Gang for their help in the field.

4. Genomic studies

Energetics

• Survival

• Swimming

• Feeding and digestion

Functional limitation

• Heart rate

• Gill surface area

• muscle

Cellular & molecular

• Mitochondria

• Enzyme activity

• Transcriptome

Whole genome resequencing

Discover candidate genes

• Population differentiation

• Trait association

• Thermal plasticity

Background

The goal of mechanism section is to identify genetic,

environmental, and phenotypic mechanisms that

translate to adaptive capacity of redband trout

(Oncorhynchus mykiss gairdneri) populations.

YEAR-1 & -2 Objectives include:

1. Collect fish from field for common garden acclimation

2. Quantify physiological responses to temperature

3. Conduct genome scan for candidate genes

Common garden 2

(sub-optimum, N=315)

Common garden 3

(stress, N=315)

15°C 19°C 23°C

Age-0 fish collected from natural streams

Cool Montane:(4) Keithley Creek(5) Whiskey Jack Creek(6) Trail Creek

Heart

Summary

Data generated from common

gardens will be used to compare

intraspecific differences in life

history traits and genotypes. It

can also be incorporated into

agent-based models to predict

adaptive capacity and

population densities under

climate change scenarios.

Desert:(1) Little Jacks Creek(2) Duncan Creek(3) William Creek

Cold Montane:(7) Upper Mann Creek(8) Fawn Creek (9) Callahan Creek

12

3

4

5

6

78

9