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Stem Cell Science
A brief update
9/10/08
Gerry Maxwell
Jaenisch and Young 2008 Cell 132:567-582.
Jaenisch and Young 2008 Cell 132:567-582
I. Park and G.Q. Daley 2007 Nature Cell biology 9: 871-873.
Jaenisch and Young 2008 Cell 132:567-582
Kinetics of reprogramming
• Reprogramming using virally encoded transcription factors in vitro takes many days and not all markers of pluripotency are activated together
• In addition, it appears that stochastic events are involved in reprogramming because clonally derived cells do not reprogram in a synchronous fashion
Creation of Patient Specific Cell Lines
• Originally published in Science Express on 31 July 2008• Science 29 August 2008:• Vol. 321. no. 5893, pp. 1218 - 1221• DOI: 10.1126/science.1158799•
• Induced Pluripotent Stem Cells Generated from Patients with ALS Can Be Differentiated into Motor Neurons
• John T. Dimos,1* Kit T. Rodolfa,1,2* Kathy K. Niakan,1 Laurin M. Weisenthal,1 Hiroshi Mitsumoto,3,4 Wendy Chung,4,5 Gist F. Croft,4,6 Genevieve Saphier,1 Rudy Leibel,5 Robin Goland,7 Hynek Wichterle,4,6 Christopher. Henderson,4,6 Kevin E Eggan1
In vivo reprogramming of adult pancreatic exocrine cells tocells
Zhou,Brown, Kanareck, Rajagopal, and Melton
2008 Nature 07134
Q Zhou et al. Nature 000, 1-6 (2008) doi:10.1038/nature07314
A combination of three transcription factors inducesinsulin+ cells in adult mouse pancreas in vivo.
Q Zhou et al. Nature 000, 1-6 (2008) doi:10.1038/nature07314
Induced new b-cells remodel vasculature and ameliorate hyperglycaemia.
Characteristics of in vivo
reprogramming • Like in vitro the inducing factors are only
required transiently• Does not appear to require cell division• Occurs as early as 3 days-faster than in vitro (7-
30 days in vitro)• Efficiency is up to 20%- much higher than in vitro• Does not appear to involve dedifferentiation as
does in vitro reprogramming