Cyrus automates a set of Rosetta protein design software protocols that have been refined and tested on experimental data sets. Cyrus Bench® is an easy-to-use, SaaS offering proven to reliably perform essential tasks in protein engineering and modeling for consumer products applications.

•  Applications in Green Chemistry: Enzyme engineering for biocatalytic production of feedstock molecules

•  Applications in small molecule discovery: Scents and other bio-active small molecules

•  Highest Accuracy Protein Structure Prediction / Homology Modeling – Useful in enzyme engineering

•  Protein Stabilization - via single and multiple point mutations and evolutionary redesign

•  Fast Free Energy Calculations - ΔΔG, broadly useful in protein engineering

•  Loop Modeling and Design - optimization or de novo

•  Identification of Alternative Protein Structure Conformations – Applications in small molecule discovery

•  Enzyme Optimization

Rosetta modeling for Consumer Products Validated with over 1000 publications

cyrusbio.com

(1) Song, Y. et al., "High-Resolution Comparative Modeling with RosettaCM". Structure (2013) 21, 1735.(2) Kellogg et al., “Role of conformational sampling in computing mutation-induced changes in protein structure and stability”. Proteins (2010) 79, 830.(3) Borgo et al., ”Automated selection of stabilizing mutations in designed and natural proteins". PNAS (2012) 109, 1494.(4) Tyka et al., “Alternate states of proteins revealed by detailed energy landscape mapping”. J Mol Biol (2011) 405(2):607-18.(5) Ollikainen, de Jong and Kortemme, “Coupling Protein Side-Chain and Backbone Flexibility Improves the Re-design of Protein-Ligand Specificity”. PLoS Comput Biol (2015) 11(9)(6) Stein and Kortemme, “Improvements to Robotics-Inspired Conformational Sampling in Rosetta”. PLOS ONE (2013) 8(5): e63090

Loop Modeling & Design(6)

Alternative Conformations(4)

Stabilization

(ΔΔG) (2)(3)

Homology Modeling(1)

Enzyme Optimization(5)

Accurate Models of very low homology structures

•  Easy to Use Graphical User Interface

•  Massively Scalable Cloud Implementation

•  No Installation Required

•  Runs in Chrome Browser

•  Full-Time Professional Support

in silico Protein Engineering using Rosetta Validated with over 1000 publications

cyrusbio.com