Rayat Shikshan Sanstha’s

Karmaveer Bhaurao Patil College Vashi

Autonomous College

Syllabus for Approval

Sr.

Heading Particulars No.

1 Title of Course F.Y.B.Sc. Microbiology

2 Eligibility for Admission 12th Science and equivalent

[of recognized Boards]

3 Passing Marks 40%

4 Ordinances/Regulations

(if any) -

5 No. of Years/Semesters One year/Two semester

6 Level U.G.

7 Pattern Semester

8 Status Revised

9 To be implemented from

2018-2019 Academic year

AC- / / 2018

Item No-

Rayat Shikshan Sanstha’s

KARMAVEER BHAURAO PATIL COLLEGE VASHI

(AUTONOMOUS COLLEGE) Sector-15- A, Vashi, Navi Mumbai - 400 703

Syllabus for F.Y.B.Sc. Microbiology

Program: B.Sc. Microbiology

Course: F.Y.B.Sc. Microbiology

(Choice Based Credit, Grading and Semester System with effect from the academic year 2018‐2019)

Preamble

Bachelor of Science (B.Sc.) in Microbiology is an under graduation programme of Department

of Microbiology, Karmaveer Bhaurao Patil College Vashi, Navi Mumbai [Autonomous College] Microbiology is a branch of science that studies microorganisms such as bacteria, protozoa,

algae, fungi, bacteria, viruses, etc. These studies integrate cytology, physiology, ecology,

genetics and molecular biology, evolution, taxonomy and systematics with a focus on

microorganisms.

The relevance and applications of microorganisms to the surrounding environment including

human life and Mother Nature becomes part of this branch. Since inception of this branch of

science, Microbiology has remained a field of active research and ever expanding in all possible

directions; broadly categorized as pure and applied science.

Different branches of Pure Microbiology based on taxonomy are Bacteriology, Mycology,

Protozoology and Parasitology, Phycology and Virology; with considerable overlap between

these specific branches over each other and also with other disciplines of life sciences, like

Biochemistry, Botany, Zoology, Cell Biology, Biotechnology, Nanotechnology, Bioinformatics,

etc. Areas in the applied Microbial Sciences can be identified as: Medical, Pharmaceutical, Industrial

(Fermentation, Pollution Control), Air, Water, Food and Dairy, Agriculture (Plant Pathology and

Soil Microbiology), Veterinary, Environmental (Ecology, Geomicrobiology); and the

technological aspects of these areas.

Microbiological tools have been extensively used to study different life processes and are cutting

edge technologies. There is a continual demand for microbiologists in the work force – education, industry and research. Career opportunities for the graduate students are available in

manufacturing industry and research institutes at technical level.

The content of a syllabus should be such that it maintains continuity with the course content of

higher secondary class and post graduate course. The present curriculum is made keeping this in

mind and is an effort to impart fundamental knowledge of the subject needed at this level. The

curriculum is designed as per the guidelines for Choice Based Credit System and reflects the

total credit, teaching hours and evaluation pattern.

Objectives of the Course:

• To enrich students’ knowledge and train them in the pure microbial sciences

• To introduce the concepts of application and research in Microbiology

• To inculcate sense of scientific responsibilities and social and environment awareness

• To help students’ build-up a progressive and successful career

Course Learning Outcome:

By the end of the course, a student should develop the ability to

1. Explain, coherently and effectively various genres of microbiology.

2. Discuss and compare prokaryotic and eukaryotic morphology.

3. Develop basic skills in practical microbiology.

4. Investigate nutritional needs of microorganisms.

5. Use different methods of control of microorganisms.

6. Examine various biomolecules.

7. Elaborate on diversity of microbial ecosystems.

8. Restate basic concepts of immunity.

9. Illustrate scope for industrial microbiology and basics of fermentation.

Scheme of examination for Each Semester: Continuous Internal Evaluation: 40 Marks (Common Test-20 Marks & 20 Marks for-

Assignment, Projects, Group discussion, Open book test, online test etc.) based on Unit III of

each paper.

Semester End Examination: 60 Marks will be as follows -

Theory: The Semester End Examination for theory course work will be conducted as

per the following scheme.

Each theory paper shall be of two hours duration.

All questions are compulsory and will have internal options.

I.

Q – I Subject questions from Unit – I (having internal options.) 20 M

Q – II Subjective questions from Unit – II (having internal options.) 20 M

Q – III Objective type questions based on both the Units with equal weightage.

20 M

Practical

The Semester End Examination for practical course work will be

II. conducted as per the following scheme.

Sr.

Marks% No. Particulars of Semester End Practical Examination

1 Laboratory Work 80

2 Journal 10

3 Viva 10

TOTAL

100

Scheme of examination for Each Semester: Continuous Internal Evaluation: 40 Marks (Common Test-20 Marks & 20 Marks for-

Assignment, Projects, Group discussion, Open book test, online test etc.) based on Unit III of

each paper.

Semester End Examination: 60 Marks will be as follows -

Theory: The Semester End Examination for theory course work will be conducted as

per the following scheme.

Each theory paper shall be of two hours duration.

All questions are compulsory and will have internal options.

I.

Q – I Subject questions from Unit – I (having internal options.) 20 M

Q – II Subjective questions from Unit – II (having internal options.) 20 M

Q – III Objective type questions based on both the Units with equal weightage.

20 M

Practical

The Semester End Examination for practical course work will be

II. conducted as per the following scheme.

Sr.

Marks% No. Particulars of Semester End Practical Examination

1 Laboratory Work 80

2 Journal 10

3 Viva 10

TOTAL

100

F. Y. B. Sc. Microbiology

SEMESTER I

Paper I [UG MB-101]: Fundamentals of Microbiology

Paper II [UG MB-102]: Basic Techniques and Applied Microbiology

Course Code Unit Topics Credits L /

Week

I Introduction to Microbiology & Prokaryotic 1

UG MB-101 Cell Structure

II Eukaryotic Cell Structure & function 2

1

III Microbial Evolution &Diversity 1

I Nutrition and Growth of Microorganisms 1

and Impact of Microorganisms

UG MB-102 II Control of microorganisms

2 1

III Basic Instrumentation 1

MBP-1 Practicals Basic Techniques of 2 6

Microbiology

SEMESTER II

I Basics of Biochemistry 1

UG MB-201

2

II Basic Virology 1

III Microbial ecosystem 1

I Introduction to Industrial Microbiology 1

UG MB-202

2

II Basic Immunology 1

III Infections 1

MBP-2 Practicals Methods in Microbiology 2 6

F.Y.B.Sc. Microbiology

Credit Based Semester & Grading System

2018-19

SEMESTER I Paper I

Course

Code

Title Credits

UGMB-

101

Fundamentals of Microbiology

2 Credits

(45 lectures)

Unit-I Introduction to Microbiology & Prokaryotic Cell Structure

1.1 Introduction to Microbiology

1.2 Microbes in our lives

1.3 History of Microbiology

1.4 Types of Microorganisms (Brief)

a. Bacteria

b. Archea

c. Fungi

d. Protozoa

e. Algae

f. Viruses

1.5 Microbiology in India

1.6 Classification of organisms & Basic Concepts of

Taxonomy

a. Five Kingdom System of Classification

b. Eight Kingdom System of Classification

c. Three Domain System of Classification

1.7 Prokaryotic Cell Structure & function

a. Cell wall

b. Cell membrane

c. Components external to cell wall-

capsule, slime layer, s layer, flagella, pili, fimbriae

d. Cytoplasmic matrix-Inclusion bodies, magnetosomes,

ribosomes, gas vesicles

e. Bacterial endospores

15 Lectures

Unit-II 2.1 Eukaryotic Cell Structure & function

a. Overview of Eukaryotic cell structure

b. The plasma membrane and membrane structure

c. Cytoplasmic matrix, microfilaments, intermediate

filaments, and microtubules

d. Organelles of the Biosynthetic-secretory and endocytic

pathways –endoplasmic reticulum &golgi apparatus.

Definitions of Lysosome, Endocytosis, Phagocytosis,

Autophagy, Proteasome

e. Eukaryotic ribosomes

f. Mitochondria

g. Chloroplasts

h. Nucleus –nuclear structure

i. External Cell organelles: cilia and flagella

j. Comparison of Prokaryotic and Eukaryotic Cells

15 Lectures

Unit-III Microbial Evolution &Diversity

3.1 Evolution of Microorganisms & Current Scenario

3.2 Diversity of Microbial World – I

a. Ricketssiae

b. Coxiella

c. Chlamydia

d. Mycoplasma

e. Actinomycetes

f. Archae

3.3 Diversity of Microbial World – II

a. Fungi

b. Algae

c. Protozoa

15 Lectures

F.Y.B.Sc. Microbiology

Credit Based Semester & Grading System

2018-19

SEMESTER I Paper II

Course

Code

Title Credits

UGMB-

102

Techniques & Applied Microbiology 2 Credits

(45 lectures)

Unit-I 1.1 Nutrition and Growth of Microorganisms

a. Nutrition: Macronutrients & Micronutrients including

growth factors

b. Table for general physiological functions

c. Types of Microorganisms based on nutritional

requirements

d. Uptake of nutrients: Passive diffusion, Facilitated

diffusion, Active transport, Group translocation (only

definitions)

e. Cultivation & Detection of Microorganisms

f. Culture media: Different types- Synthetic, Complex,

Anaerobic, Selective, Differential & Enriched medium

g. Growth: Definition, Growth Curve, Continuous Culture,

Measuring Microbial Growth, Quantitative Measurements:

Direct &Indirect Methods

h. Effects of solutes, osmotic tolerance, Temperature, Oxygen

1.2 Impact of Microorganisms

Impact of Microorganisms in various fields

a. Microbes in Food & Dairy Industries

b. Microbes in Production of Industrial Products

c. Microbes in Genetic Engineering & Biotechnology

d. Microbes in Environmental Microbiology

e. Microbes & Medical Microbiology

f. Microbes in Agriculture

g. Microbes in Bio-terrorism

h. Bioinformatics

i. Bioprocess Technology

15 Lectures

Unit-II 2.1 Control of microorganisms a. Definitions of frequently used terms

b. Physical

i. Dry and Moist heat- mechanisms, instruments used

and their operations

ii. Electromagnetic radiations-Ionizing radiations,

mechanism- advantages and disadvantages

iii. Bacterial proof filters

iv. Low temperature

v. Osmotic pressure

vi. Desiccation

15 Lectures

c. Chemical methods

i. Phenolics

ii. Alcohols

iii. Heavy metals and their compounds

iv. Halogens

v. Quaternary ammonium compounds

vi. Dyes

vii. Surface active agents/Detergents

viii. Aldehydes

ix. Peroxygens

x. Sterilizing gases

d. Biological Methods

e. Conditions influencing the effectiveness of antimicrobial

agents

f. Mechanisms of cell injury

2.2 Biosafety cabinets

Unit-III Basic Instrumentation

3.1 Microscopy

a. Compound Microscope and types

b. Electron microscope

c. New generations of Microscopes-Atomic Force microscopy

and SEM Include names

d. Care & use of Microscope

3.2 pH meter

3.3 Colorimeter

3.4 Centrifuge and types

3.5 Shaker and types

15 Lectures

F.Y.B.Sc. Microbiology Practicals

Credit Based Semester & Grading System

2018-19

Course

code

Title Credits

UGMB

P1

Section I- Basic Techniques and Applied Microbiology 1 Credit

(45 Lectures)

1. Orientation to Microbiology laboratory

2. Introduction to safety measures in laboratory and disposal of

microbial cultures

3. Microscopy- Study of Compound Microscope and its parts

Care and Maintenance of microscope

4. Study of Microscopic Morphology of Microorganisms:

a. Monochrome staining

b. Gram Staining

c. Hanging drop method (motility)

5. Special staining:

a. Capsule Staining

b. Cell Wall Staining

c. Endospore Staining

d. Metachromatic granules Staining

e. Lipid Staining

6. Cultivation of Microorganisms:

a. Preparation of Culture media-Nutrient Agar, Nutrient

Broth, MacConkey, Mannitol Salt Agar,

Sabouraud’sAgar

b. Inoculation techniques using Liquid media, Solid media-

Slant, Butt and Plate

7. Sterilization of Glassware and Culture media

a. Autoclave

b. Hot Air Oven

c. Bacteriological filter

8. Isolation of microorganisms

a. Streak Plate Method

b. Pour Plate method

c. Spread Plate method

9. Enumeration of Microorganisms

a. Viable count: Spread plate method and Pour plate

method

b. Haemocytometer

c. Breed’s Count Method

d. Opacity Tube Method

F.Y.B.Sc. Microbiology

Credit Based Semester & Grading System

2018-19

SEMESTER II Paper I

Course

Code

Title Credits

UGMB-

201

Biochemistry, Virology and Microbial ecosystem

1 Credits

(45 lectures)

Unit-I Basics of Biochemistry

1.1 DNA structure

a. Nitrogenous bases-Purines and Pyrimidines

b. Pentoses-ribose, deoxyribose

c. Nomenclature of nucleosides and nucleotides,

d. N-Beta glycosidic bond, polynucleotide chain to show

bonding between nucleotides (phosphodiester bonds)

1.2 Basic structure of RNA and DNA

1.3 Chemical foundation

a. Types of bonds and their importance: electrovalence,

covalent, ester, phosphodiester, thioester, peptide and

glycosidic bond

1.4 Water-Structure, properties in brief

1.5 Carbohydrates

a. Definition, Classification, Biological role

b. Monosaccharides

c. Oligosaccharides (Maltose, Cellobiose, Sucrose, Lactose)

d. Polysaccharides (Starch, Glycogen, Peptidoglycan,

Cellulose)

1.6 Lipids

a. Fatty acids as basic component of lipids and their

classification, nomenclature

b. Storage lipids and structural lipids

c. Types of Lipids with general structure of each and

mention examples

1.7 Amino acids and Proteins

a. General structure and features of amino acids (emphasis

on amphoteric nature)

b. Classification by R-group

c. Uncommon amino acids and their functions

d. Peptides and protein- Definition and general features and

examples with biological role

e. Primary, secondary, tertiary, quaternary structures of

proteins- Brief outline

15 Lectures

Unit-II Basic Virology

2.1 General Characteristics of Viruses

a. Viral structure

b. Nucleic acid, protein

c. Capsid and envelope

d. General Morphology:helical, polyhedral, enveloped,

complex

2.2 Classification of viruses (Baltimore classification)

2.3 Isolation, Cultivation and Multiplication of viruses

a. Bacteriophages: Lytic cycle, Lysogeny

b. Cultivation of bacteriophages (Phage Assay)

15 Lectures

Unit-III Microbial ecosystem 3.1 Principle of Microbial ecology

3.2 Ecological concepts

3.3 Ecosystem and biogeochemical cycling

3.4 Microbial Habitat

3.5 Environment and microenvironment

3.6 Biofilms: Microbial growth on surfaces, disadvantages and

control of biofilms

3.7 Freshwater, Soil and Plant microbial ecosystem

a. Freshwater Microbial ecosystems

b. Terrestrial Microbial ecosystems

c. Plants as microbial habitat

3.8 Animal-Microbial Symbiosis

3.9 Plant-Microbial Symbiosis

15 Lectures

F.Y.B.Sc. Microbiology

Credit Based Semester & Grading System

2018-19

SEMESTER II Paper II

Course

Code

Title Credits

UGMB-

202

Bioprocess Technology, Immunology and Medical

Microbiology

2 Credits

(45 lectures)

Unit-I 1.1 Introduction to Industrial Microbiology

Definition and scope of industrial Microbiology

1.2 Culture, components of media, types of media

1.3 Microbial Growth (Definition, Generation Time, Growth rate,

Phases of Growth, Chemostat, turbidostat)

1.4 Inoculum Development: Preparation of inoculum

1.5 Sterilization: media, equipment, fermentors

1.6 Fermentation: Types of fermentors, batch and continuous

fermentation, surface and submerged fermentation

1.7 Probiotics and prebiotics

15 lectures

Unit-II Basic Immunology

2.1 Introduction to Immunology: definition, types of immunity

innate- anatomic barriers, physiological and chemical barriers

and adaptive immunity, inflammation, Cells of Immune system-

- T cell, B cell, Natural Killer cell, Dendritic Cell,

Macrophages, Neutrophiles, Eosinophiles, Basophiles

2.2 Antigen: definition, types of antigen, epitope, hapten, adjuvant,

antigenicity and immunogenicity, foreignness of antigen

2.3 Antibody: definition, types, structure (diagram only) and

Antigen-Antibody reaction- Introduction to precipitation and

Agglutination

2.4 Types of Immune Response- Humoral and Cell Mediated

Immune Response

2.5 Primary and secondary lymphoid organs (Emphasis on

diagram)

15 lectures

Unit-III Infections

3.1 Infections: definition of medical terms, introduction,

classification of infection (primary and secondary) modes of

transmission of infection, sources of infection

3.2 Virulence Factors of pathogens: Enzyme, Toxins

3.3 Emerging Infections: Factors affecting emerging infections

3.4 Examples of Prevalent and Emerging Infections(Malaria, Ebola

Virus, Chikungunya, Leptospirosis, Opportunistic Infections by

E.coli, Nipah Virus, Swine flu)

15 lectures

F.Y.B.Sc. Microbiology Practicals

Credit Based Semester & Grading System

2018-19

Course

code

Title Credits

MB P2 Section II- Methods in Microbiology 2 Credit (45

Lectures)

1. Control of Microorganisms

a. Physical Methods - Temperature

b. Chemical Methods –Phenol, Antibiotic and Dyes

2. Effect of Different environmental conditions on growth of

Microorganisms

a. Temperature

b. pH

c. Oxygen (Static and shaker condition)

d. Osmotic Pressure (Salt and Sugar)

3. Isolation of Microbes from various sources- Soil, Water, Air,

Fomites, Fermented food

4. Study of viruses- Phage Assay

5. Qualitative detection of

a. Carbohydrates

b. Proteins

c. Amino acids

d. DNA

e. RNA

6. Study of Nitrogen fixers-Rhizobium and Azotobacter

7. Study of Normal flora of Skin

8. Detection of Virulence factors- Haemolysin , Coagulase and

Lecithinase

9. Detection of Blood group

10. Study of Malarial Parasite

11. Preparation of buffer and study of pH meter

12. Verification of Beer-Lambert’s Law by Colorimeter

References:

1. Madigan M.T., Martinko J.M. (2006). Brock’s Biology of Microorganisms. 11th Edition.

Pearson Education Inc.

2. Prescott L.M., Harley J.P., AND Klein D.A. (2005). Microbiology, 6th Edition.

MacGraw Hill Companies Inc.

3. Salle A.J. (1971) Fundamental Principles of Bacteriology. 7th Edition. Tata MacGraw

Publishing Co.

4. Stanier R.Y., Adelberg E.A. and Ingraham J.L. (1987) General Microbiology, 5th

Edition. Macmillan Press Ltd.

5. Tortora G.J., Funke B.R., Case C.L. (2006). Microbiology: An Introduction. 8th Edition.

Pearson Education Inc.

6. Kathleen Park Talaro & Arthur Talaro - Foundations in Microbiology International

edition 2002,| McGraw Hill.

7. Wilson K. and Walker J.M. (2005) Principles and Techniques of Biochemistry and

Molecular Biology. 6th Edition. Cambridge University Press.

8. Lehninger. Principles of Biochemistry. 4th Edition. D. Nelson and M. Cox. W.H.

Freeman and Company. New York 2005.

9. Microbiology: An Introduction. 6th Edition. Tortora, Funke and Case. Adisson Wesley

Longman Inc. 1998.

10. Manual of Microbiology: Tools & Techniques by Kanika Sharma 2nd edition.

11. General Microbiology by Powar & Daginawala Volume II.

12. A Textbook of Microbiology by R. C. Dubey & Maheshwari.

13. Microbiology by Jacquelyn Black 6 th edition.

14. Advanced Virology: Wagner 3 rd Edition.

15. Prescott, Hurley, Klein-Microbiology,9th edition, McGraw Hill. 9th Edition

16. Ananthanarayan and Panicker’s, Textbook of Microbiology, 9th edition

17. Kuby Immunology, 6th Edition, W H Freeman and Company

18. Casida L. E., "Industrial Microbiology”(2009) Reprint, New Age International (P) Ltd,

Publishers, New Delhi

19. Milk and Milk Products: Clarence Henry 4th Edition.