•

Microbiome influence on energy balance and

brain development/function put into action to

tackle diet-related diseases and behaviour

Coordinator: Yolanda Sanz, IATA-CSIC (Spain)

Co-coordinator: Patrizia Brigidi, UNIBO (Italy)

Final Conference 18 October 2018

Grant agreement nº 613979

Gut microbiome role in metabolism, brain function, obesity and behaviour

• Funded under the call KBBE.2013.2.2.-02 7FP

• Large Collaborative Project

• Project duration: 5 years

• Start date: 1 December 2013 until 30 Nov 2018

• EU contribution ≅ € 9 million

• Multi-disciplinary and multi-sectorial consortium:

experts in microbiology, nutrition, physiology,

immunology, neuroscience, omics, systems biology and

computational modelling, food industry, associations.

30 Partners of 15 EU and non-EU countries

ObesityEating &Mood

disorders

The burden of obesity and behavioural disorders

Childhood Obesity Surveillance Initiative (2017) -Number of obese children has

increased tenfold in the last 40 years. Mental disorders

major cause of disability

“The globalized food system is not delivering the diets necessary for healthy living”(GAIN, WHO, FAO, Sept 2018, Rome)

Microbiota and diet’s roles in metabolic health, obesity & behaviour

Eating behaviour

OVEREATING

Western diet

Sedentary life

Highly palatable foodsOBESITY

High energy intake

Stress, sleep pattern, food preferences

Emotional behaviour and Mood

Diet-Microbiota

• Metabolic Risk

Gut microbiota and diet in metabolic health, obesity & behaviour in humans

1 Does protein source matters?

Proteins

Fibres

PUFAS

Causality - MetS

•Microbiota vs butyrate

2

Intervention trials in humans

O=

OH

=

OH

Butyrate

+

O=OH

=

OH

SerotoninDopamine

Frontal cortex

O=

OH

=

OH

Donor microbiota’s effect

INRA-CSIC-UREAD-URG

AMC-CSIC-UREAD-URG

O=OH

=

OH

O=OH

=

OH

Obesity prediction

• Microbiome

• Diet, Exercise

• Inflammation

Gut microbiota and diet in metabolic health, obesity & behaviour

3

Eating behaviour

4

Observations studies in humans

UNIBO

4 years

Biomarker development

Control

womenObese

Obese

Food-addictive

behaviour

Reward vs

homeostatic

pathways

UNIBO-BIPS-CSIC

Cultivating

uncultured bacteria

200 from

50 new spp.

Gut microbiota and diet in metabolic health, obesity & behaviour in models

1

Effectivenes in DIO

• B. uniformis 7771

• B. unif + fibres

• NGP (patents)

2

NGP selection & mechanistic studies

CSICInteractions in gut ecosystem

LEAN

OBESE

OBESE

OBESE

ILC3

ILC1

IL-23

INF-

Treg

TGFβ

IL-33/TSLP

IL-10

CSIC-URG

PPARγ “Brown genes”

Thermognesis

Cp1aATP

β-oxidation

B. uniformis + Fibre

whole-genome

DPPIV & Metabolites

Gut microbiota and diet in metabolic health, obesity & behaviour in models

3

Obesity& Emotions

4

Mechanisms of gut-liver-brain communication

UCL/TUM-MUG-CSICGut – liver axis

OBESE

▪ Gut microbiota

▪ Antimicrobials

▪ Liver inflammation

GERMFREE

LEAN

MUG-UREAD-UCL-CSIC

Gut – brain axis

DPPIV

NPY

GLP1

Cytokines

▪ Gut microbiota

▪ Brain metabolites

Early microbiota, immune and brain

development & role in health programming

METABOLIC HEALTH

MENTAL HEALTH

Diet

Stress

Drugs

Birth type

InfectionsANS

HPA axis

Immune cells

Entericneurons

EpithelialEEC

SIG

NA

LLIN

G

Neural

Edocrine

Immune

Window of Opportunity Childhood & Adulthood

Lifestyle &

microbiota

Early microbiota, immune and brain development & role in health programming

1

Lifestyle & microbiota

2

From pregnancy to infancy, childhood and adulthood

UCC-UREAD

OBESE

OBESE

UGR-UNIBO-CSIC

NEURODEVELOPMENT&

OBESITY

IMMUNITY&

STRESS • Maternal obesity- diabetes

• Delivery mode

• Antibiotic intake

• Dietary patters

MICROBIOME MODULATORS

ASSESMENTS

• Omics

• Immune-Endocrine markers

• Brain imaging

• Behaviour

• Neurodevelopment

INFANTMET

PREOBE

“connectome”

Innovation activities to translate microbiome science to industry and consumers

• Evidence-based information for consumers

• Scaling production for efficacy trials

• Development of innovative food prototypes

• Selection of functional ingredients

CAPSA, CARGIL, AH, ADMS, FINS & LALLEMAND

Probiotics & brain

Effectiveness of foods & ingredients targeting the gut on metabolic & mental health

1

Fiber & metabolism

2

Randomized Control Trials in humans

UCC-AH

OBESE

OBESE

UCPH-CAPSA

PROBIOTIC

PLACEBO

MILK ENRICHED WITH FIBER

MILK -PLACEBO

RESILIENCE TO STRESS

OBESITY AND RISK MARKERS

Exploitation of results and knowledge

• Next Generation Probiotics-Live Bio-Terapeutics (CSIC)• Biomarkers for disease prediction (CSIC,UNIBO)• Healthy foods & ingredients (CAPSA, AH, UCHP, UCC, etc.)• Start-Up: Wellmicro (UNIBO)

• Support health policies

• Inform future dietary recommendations

• Information to facilitate consumer’s choices

(EUFIC, ICC, TNO, etc.)

Knowledge

POLICIES & CONSUMERS

INDUSTRIAL COMPETITIVENES

• Datasets (8 + 4 studies)

• Phenotype database (TNO)

• Standards

• Analytical tools:

Lipidomics (UHR)

Long-amplicon seq (CSIC)

FOSTERING SCIENCE

Marketable Products

Public

HealthKnowledge

Further EU R&D Activities

7+ New R&D ProjectsMICROBIOMESUPPORT,

CIRCLES, MIVAO, ERA/JPI-HDH, etc.

(CSIC, CARG, UNIBO, UCC, EUFIC

MUG, UCC, UHR))

14 PhD students, 13 Postdocs,

many undergraduates Training & education activities

Image from openPR.com

Scientific Advisory BoardAntony Leeds,

Garrath Williams, etc.

Dissemination highlights

www.mynewgut.eu

webpage views109K+

558 e-newsletter subscribers

Food Todayarticle & leaflet

6.5K+ followers

@MyNewGut

1.5K+ likes

>200 presentations at events

48 scientific publications

Special Issue & Opinion Papers

TV & podcast interviews

10 press articles

Thanks all MyNewGut partners and SAB

Microbiome Influence on Energy Balance and Brain Development and/or Function Put into Action to

Tackle Diet-Related Diseases has received funding from the European Union’s Seventh Framework

Programme for research, technological development and demonstration. Grant Agreement no: 613979

Thank you!