•
Microbiome influence on energy balance and
brain development/function put into action to
tackle diet-related diseases and behaviour
Coordinator: Yolanda Sanz, IATA-CSIC (Spain)
Co-coordinator: Patrizia Brigidi, UNIBO (Italy)
Final Conference 18 October 2018
Grant agreement nº 613979
Gut microbiome role in metabolism, brain function, obesity and behaviour
• Funded under the call KBBE.2013.2.2.-02 7FP
• Large Collaborative Project
• Project duration: 5 years
• Start date: 1 December 2013 until 30 Nov 2018
• EU contribution ≅ € 9 million
• Multi-disciplinary and multi-sectorial consortium:
experts in microbiology, nutrition, physiology,
immunology, neuroscience, omics, systems biology and
computational modelling, food industry, associations.
30 Partners of 15 EU and non-EU countries
ObesityEating &Mood
disorders
The burden of obesity and behavioural disorders
Childhood Obesity Surveillance Initiative (2017) -Number of obese children has
increased tenfold in the last 40 years. Mental disorders
major cause of disability
“The globalized food system is not delivering the diets necessary for healthy living”(GAIN, WHO, FAO, Sept 2018, Rome)
Microbiota and diet’s roles in metabolic health, obesity & behaviour
Eating behaviour
OVEREATING
Western diet
Sedentary life
Highly palatable foodsOBESITY
High energy intake
Stress, sleep pattern, food preferences
Emotional behaviour and Mood
Diet-Microbiota
• Metabolic Risk
Gut microbiota and diet in metabolic health, obesity & behaviour in humans
1 Does protein source matters?
Proteins
Fibres
PUFAS
Causality - MetS
•Microbiota vs butyrate
2
Intervention trials in humans
O=
OH
=
OH
Butyrate
+
O=OH
=
OH
SerotoninDopamine
Frontal cortex
O=
OH
=
OH
Donor microbiota’s effect
INRA-CSIC-UREAD-URG
AMC-CSIC-UREAD-URG
O=OH
=
OH
O=OH
=
OH
Obesity prediction
• Microbiome
• Diet, Exercise
• Inflammation
Gut microbiota and diet in metabolic health, obesity & behaviour
3
Eating behaviour
4
Observations studies in humans
UNIBO
4 years
Biomarker development
Control
womenObese
Obese
Food-addictive
behaviour
Reward vs
homeostatic
pathways
UNIBO-BIPS-CSIC
Cultivating
uncultured bacteria
200 from
50 new spp.
Gut microbiota and diet in metabolic health, obesity & behaviour in models
1
Effectivenes in DIO
• B. uniformis 7771
• B. unif + fibres
• NGP (patents)
2
NGP selection & mechanistic studies
CSICInteractions in gut ecosystem
LEAN
OBESE
OBESE
OBESE
ILC3
ILC1
IL-23
INF-
Treg
TGFβ
IL-33/TSLP
IL-10
CSIC-URG
PPARγ “Brown genes”
Thermognesis
Cp1aATP
β-oxidation
B. uniformis + Fibre
whole-genome
DPPIV & Metabolites
Gut microbiota and diet in metabolic health, obesity & behaviour in models
3
Obesity& Emotions
4
Mechanisms of gut-liver-brain communication
UCL/TUM-MUG-CSICGut – liver axis
OBESE
▪ Gut microbiota
▪ Antimicrobials
▪ Liver inflammation
GERMFREE
LEAN
MUG-UREAD-UCL-CSIC
Gut – brain axis
DPPIV
NPY
GLP1
Cytokines
▪ Gut microbiota
▪ Brain metabolites
Early microbiota, immune and brain
development & role in health programming
METABOLIC HEALTH
MENTAL HEALTH
Diet
Stress
Drugs
Birth type
InfectionsANS
HPA axis
Immune cells
Entericneurons
EpithelialEEC
SIG
NA
LLIN
G
Neural
Edocrine
Immune
Window of Opportunity Childhood & Adulthood
Lifestyle &
microbiota
Early microbiota, immune and brain development & role in health programming
1
Lifestyle & microbiota
2
From pregnancy to infancy, childhood and adulthood
UCC-UREAD
OBESE
OBESE
UGR-UNIBO-CSIC
NEURODEVELOPMENT&
OBESITY
IMMUNITY&
STRESS • Maternal obesity- diabetes
• Delivery mode
• Antibiotic intake
• Dietary patters
MICROBIOME MODULATORS
ASSESMENTS
• Omics
• Immune-Endocrine markers
• Brain imaging
• Behaviour
• Neurodevelopment
INFANTMET
PREOBE
“connectome”
Innovation activities to translate microbiome science to industry and consumers
• Evidence-based information for consumers
• Scaling production for efficacy trials
• Development of innovative food prototypes
• Selection of functional ingredients
CAPSA, CARGIL, AH, ADMS, FINS & LALLEMAND
Probiotics & brain
Effectiveness of foods & ingredients targeting the gut on metabolic & mental health
1
Fiber & metabolism
2
Randomized Control Trials in humans
UCC-AH
OBESE
OBESE
UCPH-CAPSA
PROBIOTIC
PLACEBO
MILK ENRICHED WITH FIBER
MILK -PLACEBO
RESILIENCE TO STRESS
OBESITY AND RISK MARKERS
Exploitation of results and knowledge
• Next Generation Probiotics-Live Bio-Terapeutics (CSIC)• Biomarkers for disease prediction (CSIC,UNIBO)• Healthy foods & ingredients (CAPSA, AH, UCHP, UCC, etc.)• Start-Up: Wellmicro (UNIBO)
• Support health policies
• Inform future dietary recommendations
• Information to facilitate consumer’s choices
(EUFIC, ICC, TNO, etc.)
Knowledge
POLICIES & CONSUMERS
INDUSTRIAL COMPETITIVENES
• Datasets (8 + 4 studies)
• Phenotype database (TNO)
• Standards
• Analytical tools:
Lipidomics (UHR)
Long-amplicon seq (CSIC)
FOSTERING SCIENCE
Marketable Products
Public
HealthKnowledge
Further EU R&D Activities
7+ New R&D ProjectsMICROBIOMESUPPORT,
CIRCLES, MIVAO, ERA/JPI-HDH, etc.
(CSIC, CARG, UNIBO, UCC, EUFIC
MUG, UCC, UHR))
14 PhD students, 13 Postdocs,
many undergraduates Training & education activities
Image from openPR.com
Scientific Advisory BoardAntony Leeds,
Garrath Williams, etc.
Dissemination highlights
www.mynewgut.eu
webpage views109K+
558 e-newsletter subscribers
Food Todayarticle & leaflet
6.5K+ followers
@MyNewGut
1.5K+ likes
>200 presentations at events
48 scientific publications
Special Issue & Opinion Papers
TV & podcast interviews
10 press articles
Thanks all MyNewGut partners and SAB
Microbiome Influence on Energy Balance and Brain Development and/or Function Put into Action to
Tackle Diet-Related Diseases has received funding from the European Union’s Seventh Framework
Programme for research, technological development and demonstration. Grant Agreement no: 613979
Thank you!