lab fume hood

7/30/2019 lab fume hood

1/78

Laboratory Ventilation SafetyLaboratory Ventilation Safety

J. Scott WardJ. Scott Ward


2/78

In 1925, LaboratoryIn 1925, Laboratory

Construction Company wasConstruction Company was

born. The first product was aborn. The first product was aKjeldahl NitrogenKjeldahl Nitrogen

Determination Apparatus. WeDetermination Apparatus. We

may have shortened our name,may have shortened our name,

but webut weve expanded ourve expanded our

horizons. We offer 16 differenthorizons. We offer 16 different

product lines to universities,product lines to universities,

research centers, hospitals,research centers, hospitals,

general laboratories andgeneral laboratories and

governmental agencies aroundgovernmental agencies around

the world.the world.

Labconco CorporationLabconco Corporation


3/78

Laboratory VentilationLaboratory Ventilation


4/78


5/78

History of Fume HoodsHistory of Fume Hoods

zz Thomas Edison LaboratoriesThomas Edison Laboratories

zz Fireplace and chimneyFireplace and chimneyzz Shelves outside the windowShelves outside the window

zz fume cupboardfume cupboard University ofUniversity ofLeads, England 1923Leads, England 1923

University of Leeds, EnglandUniversity of Leeds, England -- 19231923Thomas Edison, circa 1900Thomas Edison, circa 1900

Thomas Edison invented theThomas Edison invented the

incandescent light bulb in 1879.incandescent light bulb in 1879.


6/78

First Labconco Hood 1936First Labconco Hood 1936 Fiberglass 28, circa 1960Fiberglass 28, circa 1960

Fiberglass WalkFiberglass Walk--In, 1970In, 1970ss

Radioisotope, 1980Radioisotope, 1980ss

Protector Hood Line, 2002Protector Hood Line, 2002

Evolution RevolutionEvolution Revolution


7/78

Definition of a Fume HoodDefinition of a Fume Hood

A ventilated enclosureA ventilated enclosurewhere harmful or toxicwhere harmful or toxicfumes or vapors can befumes or vapors can besafely handled whilesafely handled whileprotecting theprotecting thelaboratory technician.laboratory technician.


8/78

Purpose of a Fume HoodPurpose of a Fume Hood

The primary function of aThe primary function of a

fume hood is tofume hood is tocapturecapture,,containcontain andandremoveremove

airborne contaminants.airborne contaminants.Video 9


9/78

Purpose of a Fume HoodPurpose of a Fume Hood

Fume hoods provideFume hoods provide

operator safety byoperator safety by

drawing air away fromdrawing air away from

the operator and intothe operator and intothe fume hood.the fume hood.

CVideo 11


10/78

Face VelocityFace Velocity

Definition:Definition:

Airflow into a hood isAirflow into a hood is

achieved by an exhaust blowerachieved by an exhaust blower

whichwhich pullspulls air from theair from thelaboratory room into andlaboratory room into and

through the hood and exhaustthrough the hood and exhaust

system. Thissystem. This pullpull at theat the

opening of the hood isopening of the hood is

measured as face velocitymeasured as face velocity..

Exhaust AirExhaust Air

Room AirRoom Air


11/78

Air VolumeAir Volume

Air volumeAir volume passing through a fume hood is generally equalpassing through a fume hood is generally equal

to the area of the sash opening multiplied by the averageto the area of the sash opening multiplied by the average

velocity desired. For example, if 100 feet per minute (fpm) isvelocity desired. For example, if 100 feet per minute (fpm) is

required and the hood has a sash opening of 7.5 square feet,required and the hood has a sash opening of 7.5 square feet,then the hoodthen the hoods air volume is 750 (7.5 x 100) cubic feet pers air volume is 750 (7.5 x 100) cubic feet per

minute (CFM).minute (CFM).


12/78

Design ComponentsDesign Components

Air FoilAir Foil

Aerodynamic sash openingAerodynamic sash opening

directs airflow into hood anddirects airflow into hood andacross work surface withacross work surface with

minimum turbulence helpingminimum turbulence helping

to ensure fume containment.to ensure fume containment.


13/78


TheThe SASHSASH controls the areacontrols the area

of the fume hood which isof the fume hood which is

open. It protects theopen. It protects the

operator and controls hoodoperator and controls hoodface velocities. Glass optionsface velocities. Glass options

include tempered orinclude tempered or

laminated.laminated.

SashSash


14/78

Safety Sash: Physical BarrierSafety Sash: Physical Barrier

VerticalVertical--risingrising

sashsash

HorizontalHorizontal--slidingslidingsashessashes

CombinationCombination(vertical and(vertical and

horizontal sashes)horizontal sashes)


15/78


TheThe BAFFLEBAFFLE controls the pattern of the air moving into andcontrols the pattern of the air moving into andthrough the fume hood Baffles are eitherthrough the fume hood Baffles are either fixedfixed (left photo(left photo) or) or

adjustableadjustable (right photo).(right photo).

Fixed Baffle Adjustable Baffle


16/78

Types of Fume HoodsTypes of Fume Hoods

ConventionalConventional ByBy--PassPass

AuxiliaryAuxiliary--AirAir

Reduced Air VolumeReduced Air Volume

Variable Air VolumeVariable Air Volume

High PerformanceHigh Performance


17/78

Conventional Fume HoodConventional Fume Hood

Most basic hood designMost basic hood design

Operates at constantOperates at constantexhaust volumeexhaust volume

Face velocity increases asFace velocity increases assash is loweredsash is lowered

Fiberglass 28 Hood onFiberglass 28 Hood on

Acid Storage CabinetAcid Storage Cabinet


18/78

Conventional Fume HoodConventional Fume Hood

Exhaust AirExhaust Air Exhaust AirExhaust Air

Room AirRoom Air

Room AirRoom Air

With Sash OpenWith Sash Open With Sash Nearly ClosedWith Sash Nearly Closed

ByBy--Pass BlockPass Block


19/78

ByBy--Pass Fume HoodPass Fume Hood

Relatively constant faceRelatively constant face

velocityvelocity

As sash is closed, hoodAs sash is closed, hooddraws air through bypassdraws air through bypass

openings, maintainingopenings, maintaining

excellent containmentexcellent containment


20/78

ByBy--Pass Fume HoodPass Fume Hood

RoomRoom

AirAir




RoomRoom

AirAir

ByBy--PassPass

OpeningsOpenings


21/78

AuxiliaryAuxiliary--Air Fume HoodAir Fume Hood

Brings in between 50Brings in between 50--70% of70% ofair volume from outsideair volume from outside

laboratorylaboratory

May be used to augmentMay be used to augmentinsufficient room airinsufficient room air

Reduces consumption of fullyReduces consumption of fullytempered room airtempered room air

Requires two duct and blowerRequires two duct and blower

systemssystems

Balance between air systemsBalance between air systemsessentialessential


22/78

Auxiliary Air Fume HoodAuxiliary Air Fume Hood

Requires two remoteRequires two remote

blowersblowers one toone toexhaustexhaust and one toand one tosupply airsupply air

Bonnet directsBonnet directs

uniform anduniform andcontinuous air flowcontinuous air flow

Exhaust and Auxiliary AirExhaust and Auxiliary Airblower systemsblower systems must be Imust be Internter--lockedlocked

Aux. AirBlower

RemoteBlower

Bonnet


23/78

Auxiliary Air Fume HoodAuxiliary Air Fume Hood

AuxiliaryAuxiliaryOutside AirOutside Air

Exhaust Air

AuxiliaryAuxiliaryOutside AirOutside Air

Room AirRoom Air

Exhaust Air


BonnetBonnet

Room AirRoom Air


24/78

Special Purpose HoodsSpecial Purpose Hoods

PerchloricPerchloric Acid/AcidAcid/AcidDigestionDigestion

RadioisotopeRadioisotope

FloorFloor--MountedMounted

CanopyCanopy

EducationalEducational

HOPEC IVHOPEC IV


25/78

Stainless SteelStainless Steel PerchloricPerchloric Acid Fume HoodAcid Fume Hood

Wash DownWash DownSystem ControlSystem Control

Seamless oneSeamless onepiece Typepiece Type

316 stainless316 stainlesssteel linersteel liner

includingincludingwork surfacework surface

Requires dedicatedRequires dedicatedexhaust duct withexhaust duct withwash down systemwash down system

IntegralIntegraldrain troughdrain trough


26/78

PVCPVC PerchloricPerchloric Acid Fume HoodAcid Fume Hood

Wash downWash down

system controlsystem control

Seamless oneSeamless onepiece linerpiece linerincludingincluding

work surfacework surface

Requires dedicatedRequires dedicated

exhaust duct withexhaust duct withwash down systemwash down system

Integral drainIntegral draintroughtrough

Type I PVCType I PVC

linerliner


27/78

Radioisotope Fume HoodRadioisotope Fume Hood

*Work Surface*Work Surface

with integralwith integral

cupsinkcupsink

Type 304 stainlessType 304 stainless

steel interiorsteel interior

Dedicated exhaustDedicated exhaustsystem issystem isrecommendedrecommended**

** Consult local regulatoryConsult local regulatoryagencies for usageagencies for usage

recommendationsrecommendations


28/78

Canopy HoodCanopy Hood

Vents nonVents non--toxic materialstoxic materialssuch assuch as steam, heat andsteam, heat andnoxious odorsnoxious odors

May be mounted on aMay be mounted on awall or suspended fromwall or suspended fromceilingceiling

Install less than 12" aboveInstall less than 12" above

equipment to be ventilatedequipment to be ventilated


29/78

FloorFloor--Mounted Fume HoodsMounted Fume Hoods

ByBy--pass airflow designpass airflow design

Mount on floorMount on floorpermittingpermitting rollroll--inin

loading of heavy orloading of heavy or

bulk apparatusbulk apparatus

Additional interiorAdditional interiorheight to accommodateheight to accommodate

largelarge apparatusapparatus

Aerodynamic sash foilAerodynamic sash foil

* Operator should never stand inside hood* Operator should never stand inside hood

while fumes are being generated.while fumes are being generated.


30/78

XVS Ventilation StationsXVS Ventilation Stations

Student work stationStudent work station

Balance enclosureBalance enclosure

Light duty fume hoodLight duty fume hood

Solvent cleaning baySolvent cleaning bay

VeterinaryVeterinarypathology/cytologypathology/cytology

hoodhood Forensics/ latentForensics/ latent

fingerprint hoodfingerprint hood

Applications:Applications:

VS Station atop accessory work surfaceVS Station atop accessory work surface


31/78

Carbon Filtered EnclosuresCarbon Filtered EnclosuresA portable, selfA portable, self--containedcontainedenclosure for vapors and fumes,enclosure for vapors and fumes,

which requires no ducting.which requires no ducting. Applications includeApplications include

processes involving odorsprocesses involving odorsand unsafe concentrations of:and unsafe concentrations of:

99 Organic solventsOrganic solvents

99 FormaldehydeFormaldehyde

99 Acid gasesAcid gases99 AmmoniaAmmonia


32/78

Planning Laboratory SpacePlanning Laboratory Space

LocationLocation

Identify airflow configurationsIdentify airflow configurations

Adequate supply airAdequate supply air

Supply air diffuser locationSupply air diffuser location

Balanced HVAC systemBalanced HVAC system

Air changes (4Air changes (4--12 or 16 per hour for high risk)12 or 16 per hour for high risk)

Energy conservation varies geographicallyEnergy conservation varies geographically

** 300 CFM = 1 ton of air conditioning or $4 to300 CFM = 1 ton of air conditioning or $4 to

$7 dollars/CFM$7 dollars/CFM


33/78

Planning Laboratory SpacePlanning Laboratory Space

Alternate Hood

Location

Air Supply

Register

Hood

Air Supply

Register

Corridor

Airflow

Casework

Block register opening

facing hood

Each hood affects a rooms

ventilation and traffic flow, so

everything must be consideredwhen planning lab space.


34/78

MonitorsMonitors

American NationalAmerican National

Standards InstituteStandards Institute(ANSI) Standard Z9.5(ANSI) Standard Z9.5

requires the use of anrequires the use of an

airflow monitor, a deviceairflow monitor, a device

that gives warning (by athat gives warning (by avisible or audible signal,visible or audible signal,

or both) when the airflowor both) when the airflow

through the hood hasthrough the hood has

deviated from adeviated from apredetermined level.predetermined level.

DigitalDigital AnalogAnalog


35/78

Fume Hood Performance TestsFume Hood Performance Tests

SmokeSmoke


ASHRAE 110ASHRAE 110--9595

SEFASEFA


36/78


AverageAverage face velocityface velocity isiscalculated by dividingcalculated by dividing

the sash opening intothe sash opening into

oneone--foot squares.foot squares.Velocity readings areVelocity readings are

taken in each grid areataken in each grid area

and averaged.and averaged.


37/78

ASHRAE 110ASHRAE 110--9595

Three part testThree part testnot a pass/failnot a pass/fail

Face velocity profileFace velocity profile

Smoke generationSmoke generation --

Titanium tetrachlorideTitanium tetrachloride

Tracer gas containmentTracer gas containment --

Sulfur hexafluorideSulfur hexafluoride

(released at 4 liters/min.)(released at 4 liters/min.)

ASHRAE Test on FloorASHRAE Test on Floor

Mounted HoodMounted Hood


38/78

Laboratory Ventilation StandardsLaboratory Ventilation Standards

Federal Register 29 CFR Part 1910Federal Register 29 CFR Part 1910

NonNon--mandatory recommendation frommandatory recommendation from Prudent PracticesPrudent Practices

Fume hoods should have a continuous monitoringFume hoods should have a continuous monitoring

devicedevice Face velocities should be between 60Face velocities should be between 60--100 linear feet100 linear feet

per minuteper minute

Average 2.5 linear feet of hood space per personAverage 2.5 linear feet of hood space per person


39/78

Laboratory Ventilation StandardsLaboratory Ventilation Standards

Industrial VentilationIndustrial Ventilation -- ACGIHACGIH

Fume hood face velocities between 60Fume hood face velocities between 60--100 fpm100 fpm

Maximum of 125 fpm for radioisotope hoodsMaximum of 125 fpm for radioisotope hoods

Duct velocities of 1000Duct velocities of 1000--2000 fpm for vapors,2000 fpm for vapors,gasses and smokegasses and smoke

Stack discharge height 1.3Stack discharge height 1.3--2.0 x building height2.0 x building height

Well designed fume hood containment lossWell designed fume hood containment loss


40/78

BIOLOGICAL SAFETY CABINET

OPERATION

Scott Ward

LABCONCO CORP.


41/78

Working in Biological Safety CabinetsWorking in Biological Safety Cabinets

PlanningPlanningPlanning

Thoroughly understand procedures andThoroughly understand procedures and

equipment required before beginning work.equipment required before beginning work.

Arrange for minimal disruptions, such asArrange for minimal disruptions, such astraffic in the room during work.traffic in the room during work.

Have disinfectant and spill cleanup materialsHave disinfectant and spill cleanup materials

prepared.prepared.


42/78

Biological Safety Cabinet

z Contains biohazardous aerosols

z Provides personnel protection

z

May also provide product protection(depends on class)

Class II Biological Safety CabinetClass II Biological Safety Cabinet

HEPA filteredHEPA filtered


43/78

How Are Biological

Safety Cabin ets Classified?

1) Airflow velocities1) Airflow velocities

and patternsand patterns2) Exhaust system2) Exhaust system

3) Construction3) Construction

Class IClass I Class IIClass II Class IIIClass III

Defined by National Institutes of Health/Centers

for Diseases Control and Prevention (NIH/CDC)


44/78

Theory of Op eration

Biological Safety Cabinets

HEPA filtersHEPA filters

Motor/blower to force air through theMotor/blower to force air through the

cabinetcabinet

Speed control for the motor/blowerSpeed control for the motor/blower

Appropriate air intakes, ductwork, and airAppropriate air intakes, ductwork, and airbalance controlsbalance controls

Major components:Major components:


45/78

HEPA Filters

The HEPA filter is the heart ofThe HEPA filter is the heart of

the biological safety cabinet. Itthe biological safety cabinet. It

is a disposable dryis a disposable dry--type filter,type filter,

constructed of boron silicateconstructed of boron silicatemicrofibers cast into a thinmicrofibers cast into a thin

sheet. The filter media is foldedsheet. The filter media is folded

to increase its surface area.to increase its surface area.


46/78

HEPA Filters

The HEPA filter isThe HEPA filter is 99.99

efficient at removing

particles 0.3 micron orlarger in size. Gases passGases pass

freely through the filterfreely through the filter.


47/78

HEPA Filters

Filter FrameFilter Frame

Gasket SealGasket Seal

Continuous sheetContinuous sheet

of flat filterof flat filter

mediummedium

Adhesive bondAdhesive bond

between filterbetween filterpack andpack and

integral frameintegral frame


48/78

Filtration and retention ofFiltration and retention of

particulates by the HEPAparticulates by the HEPA filter(sfilter(s))

Directional airflowDirectional airflow

Laminar airflowLaminar airflow

Major principles:Major principles:

Theory of OperationTheory of Operation

Biological Safety CabinetsBiological Safety Cabinets


49/78

Theory of Operation

Directional Airflow

z Air is drawn in from the front of thecabinet. Directional airflow into the

cabinet face prevents aerosols

escaping from the face of the cabinet.

h f O i


50/78

Theory of Operation

Laminar Airflow

z Vertical laminar airflow through the workarea captures any aerosols generated in the

work area of the cabinet.

z To betrue laminar airflow, air velocities

throughout the cabinet must be + or - 20%of overall average.


51/78

Class I Biological Safety Cab inet

z Open front with directional airflow

z Operates under negative pressure

z Face velocity of 75-100 fpm

z HEPA filtered exhaust

z Provides personnel and environmental

protection onlyz For work requiring Biosafety Level 1, 2, or

3 containment

Defined By NIH/CDCDefined By NIH/CDC


52/78

Class II Biological Safety Cab inet

z Open front with directional airflow for

personnel protection

z HEPA-filtered laminar downflow for

product protection

z HEPA-filtered exhaust

zFace velocity of 75-100 fpm

z For work requiring Biosafety Level 1, 2, or 3

containment

Defined By NIH/CDC

Cl i l i l S f C b i


53/78

Class III Biological Safety Cab inets

z Totally enclosed

z Gas tight construction

z Work through gloves

z Negative pressure of at least 0.5" H2O

z Provides personnel, product and environmentalprotection

z Double HEPA filter exhaust

z For work requiring Biosafety Level 1,2, 3, or 4 containment

Defined By NIH/CDCDefined By NIH/CDC

Types of Class II


54/78

Types of Class II


B1B1

Type BType B

B2B2SubtypesSubtypes

TypesTypes

Described by NSF Standard No. 49Described by NSF Standard No. 49

Class IIClass II

Type AType A

A1A1

A2A2


55/78

Differences Between Type A and Type B


May haveMay have exposedexposed contaminatedcontaminated

positive pressure plenums (A1 only)positive pressure plenums (A1 only)

NoNo exposedexposed contaminatedcontaminated

positive pressure plenums allowedpositive pressure plenums allowed

Minimum average faceMinimum average face

velocity of 75 FPM (A1) or 100 fpm (A2)velocity of 75 FPM (A1) or 100 fpm (A2)Minimum average faceMinimum average face

velocity of 100 FPMvelocity of 100 FPM

Exhaust into lab orExhaust into lab oroutside via canopyoutside via canopy

Must exhaust outside the labMust exhaust outside the labvia dedicated exhaust systemvia dedicated exhaust system

with alarmwith alarm

Class IIClass II

Type BType A

T A T B Whi h t h ?


56/78

Type A or Type B - Which to choose?

For routineFor routine

microbiological work.microbiological work.Offers containment andOffers containment and

direct removal of volatiledirect removal of volatile

toxic gases and fumes usedtoxic gases and fumes used

in conjunction within conjunction withbiological research.biological research.

The critical factor in choosing Type A or B is not biologicalThe critical factor in choosing Type A or B is not biological

containment, but whether the user workscontainment, but whether the user works

with volatile toxic chemicals, and the volumes required.with volatile toxic chemicals, and the volumes required.

Type AType A Type BType B


57/78

ReRe--circulate into room, Type Acirculate into room, Type A

Canopy/thimble, Type A2Canopy/thimble, Type A2 Sealed connection, Type B1 or B2Sealed connection, Type B1 or B2

Exhaust OptionsExhaust Options


58/78


59/78

Installation Considerations

The major components of a biological safetycabinet exhaust system are:

z The connection to the cabinet - canopyor sealed

z A remote blower

z A backdraft damper

z A ductwork system

With gas tight damper

Cl i l i l S f C b i


60/78

Class II Biological Safety Cab inets

Type A1Type A1withoutwithoutCanopyCanopy

MinimumMinimum

inflow 75 fpminflow 75 fpm

BiosafetyBiosafety

Levels 1, 2, & 3Levels 1, 2, & 3

NoNo

radionuclidesradionuclides

No volatile toxicNo volatile toxic

chemicalschemicals

Defined by NSF Standard No. 49Defined by NSF Standard No. 49

Open flames notOpen flames not

recommendedrecommended


61/78


Type A1Type A1with Canopywith Canopy

ConnectionConnection

Minimum

inflow 75 fpm

BiosafetyBiosafety

Levels 1, 2, & 3Levels 1, 2, & 3

NoNo radionuclidesradionuclides

No volatile toxicNo volatile toxicchemicalschemicals





62/78


Type A2Type A2withoutwithout

CanopyCanopyConnectionConnection

Minimum

inflow 100 fpm

BiosafetyBiosafety

Levels 1, 2, & 3Levels 1, 2, & 3

NoNoradionuclidesradionuclides

No volatile toxicNo volatile toxicchemicalschemicals






63/78

g y

Type A2Type A2with Canopywith Canopy

ConnectionConnection

Minimum

inflow 100 fpm

BiosafetyBiosafety

Levels 1, 2, & 3Levels 1, 2, & 3

Suitable forSuitable fortrace amounts oftrace amounts of

radionuclidesradionuclides**

Suitable forSuitable forminute quantitiesminute quantitiesof volatile toxicof volatile toxic

chemicals*chemicals*




* when used as an adjunct to* when used as an adjunct tomicrobiological researchmicrobiological research



64/78

Biosafety Levels 1 , 2, & 3Biosafety Levels 1 , 2, & 3

Minute quantities of radioMinute quantities of radio--

nuclides may be used in thenuclides may be used in the

rear of the work area.rear of the work area.

Minute quantities of volatileMinute quantities of volatile

toxic chemicals may be usedtoxic chemicals may be used

in the rear of the work area.in the rear of the work area.

g y

Type B1Type B1


Minimum

inflow 100 fpm

Open flames not recommended.Open flames not recommended.



65/78


Type B2Type B2Total ExhaustTotal Exhaust

MinimumMinimum

inflow 100 fpminflow 100 fpm

BiosafetyBiosafety

Levels 1, 2, & 3Levels 1, 2, & 3

Suitable forSuitable for

radionuclidesradionuclides**

Suitable forSuitable for

volatile toxicvolatile toxic

chemicals*chemicals*

Defined by NSF Standard No. 49Defined by NSF Standard No. 49* when used as an adjunct to* when used as an adjunct to

microbiological researchmicrobiological research

Open flames notOpen flames notrecommendedrecommended

Labconco Biological Safety Cabin et


66/78

Airflow Configurations

Type A2

Type A2Type A2 Type B2Type B2

Without CanopyWithout Canopy Canopy ConnectionCanopy Connection Sealed ConnectionSealed Connection


67/78

Working in Biological Safety CabinetsWorking in Biological Safety CabinetsStart UpStart Up

Turn off UV light, open sash to its proper height,Turn off UV light, open sash to its proper height,andand turn on cabinet lights and blower.turn on cabinet lights and blower.

Check all grilles for obstructions and let theCheck all grilles for obstructions and let the

cabinet operate for 5 minutes.cabinet operate for 5 minutes.

Wash hands and arms thoroughly withWash hands and arms thoroughly with

disinfectant soap; wear a long sleeved lab coatdisinfectant soap; wear a long sleeved lab coat

with knit cuffs andwith knit cuffs and overover--thethe--cuff gloves. Use eyecuff gloves. Use eye

protection.protection.


68/78


WipeWipe--DownDown

Wipe down all interior surfaces of the workWipe down all interior surfaces of the work

area with a solution of 70% ethanol or otherarea with a solution of 70% ethanol or othersuitable disinfectantsuitable disinfectant..


69/78

Working in Biological Safety CabinetsWorking in Biological Safety CabinetsLoading

Load only the materials needed. Do notLoad only the materials needed. Do not

overload the cabinet or obstruct the grilles.overload the cabinet or obstruct the grilles.

Keep large objects separated.Keep large objects separated.

Lower the sash until it is in its proper position.Lower the sash until it is in its proper position.

Allow the unit to operate for 2 to 3 minutes toAllow the unit to operate for 2 to 3 minutes to

purge any airborne contaminants.purge any airborne contaminants.


70/78

Working in Biological Safety CabinetsWorking in Biological Safety CabinetsWork TechniquesWork Techniques

Keep all materials at least 4" inside the sash opening andKeep all materials at least 4" inside the sash opening andperform all contaminated operations as far to the rear ofperform all contaminated operations as far to the rear of

the work area as possible.the work area as possible.

Segregate clean and contaminated materials. ArrangeSegregate clean and contaminated materials. Arrangematerials to minimize movement of contaminatedmaterials to minimize movement of contaminated

materials into clean areas. Keep all contaminatedmaterials into clean areas. Keep all contaminated

material in the rear of the work area.material in the rear of the work area.

Avoid excessive movement of arms or materials throughAvoid excessive movement of arms or materials through

the front opening during operation.the front opening during operation.


71/78

Working in Biological Safety CabinetsWorking in Biological Safety CabinetsWork TechniquesWork Techniques

Use proper aseptic technique.Use proper aseptic technique.

Avoid techniques that disrupt airflow patterns inAvoid techniques that disrupt airflow patterns in

thethe

cabinet, such as an open flame.cabinet, such as an open flame.

If there is a spill or splatter during use, all objectsIf there is a spill or splatter during use, all objects

must be decontaminated before removal.must be decontaminated before removal.

Thoroughly disinfect the interior surfaces of theThoroughly disinfect the interior surfaces of thecabinet while it is still in operation.cabinet while it is still in operation.

Working in Biological Safety Cabinets


72/78


Final PurgingFinal Purging

After completing work, allow the cabinetAfter completing work, allow the cabinet

to operate for 2 to 3 minutes undisturbedto operate for 2 to 3 minutes undisturbedto purge airborne contaminants from theto purge airborne contaminants from the

work area.work area.



73/78


WipeWipe--DownDown

Periodically lift the work surface and cleanPeriodically lift the work surface and cleanunderneath it. Clean the towel catch.underneath it. Clean the towel catch.

Dispose of rubber gloves and have lab coatDispose of rubber gloves and have lab coat

properly laundered. Wash arms and handsproperly laundered. Wash arms and handsthoroughly with germicidal soap.thoroughly with germicidal soap.

Wipe down all interior surfaces of the work areaWipe down all interior surfaces of the work area

with a solution of 70% ethanol or other suitablewith a solution of 70% ethanol or other suitable

disinfectant.disinfectant.



74/78


ShutdownShutdown

Turn off the fluorescent light and cabinetTurn off the fluorescent light and cabinet

blower, close the sash and turn on the UVblower, close the sash and turn on the UVlight if appropriate.light if appropriate.

Ergonomic Considerations


75/78

Ergonomic ConsiderationsErgonomic Considerations

Feet, Knees and LegsFeet, Knees and Legs

Make sure feet rest solidly onMake sure feet rest solidly onthe floor or footrest. Donthe floor or footrest. Dontt

dangle feet or compress thighs.dangle feet or compress thighs.

Provide enough leg room underProvide enough leg room underthe cabinet to sitthe cabinet to sit comfortably.comfortably.

Vary leg and foot positionsVary leg and foot positions

throughout the day.throughout the day. Get up periodically and takeGet up periodically and take

brief walks.brief walks.

Ergonomic Considerations


76/78

Ergonomic ConsiderationsErgonomic Considerations

BackBack

Use chair to fully supportUse chair to fully supportyour body. Make sure theyour body. Make sure the

lower back is supported.lower back is supported.

DonDon

tt

slouch forward.slouch forward.

If chair is adjustable,If chair is adjustable,

experiment with theexperiment with the

adjustments to find severaladjustments to find severalcomfortable positions.comfortable positions.


77/78


78/78

Documents

lab fume hood