INBIT INDIAN BIOSCIENCES INSTITUTE OF TECHNOLOGY

534, Udyog Vihar Phase – V, Gurgaon – 122016, Haryana, India. Phone: 0124 – 4256088 Mobile: 09818779747 | Email: inbit@inbdna.com | Website: www.inbit.inbdna.com

Training Programs - 2011

Module – 1 Duration: 4 weeks Fee: Rs. 6,000/- Conceptual Basics & Scope of Biotechnology Genes, Nucleic Acid, Nucleotides & Nucleosides, DNA Polymerase, Genetic code, Alleles. History Present scenario Ongoing biotech projects Introduction to biotech laboratory Good Laboratory Practices Basic instrument handling Solution preparation

Basic Molecular biology techniques & applications Isolation & Visualization of Nucleic acid DNA isolation from plant source DNA isolation from buccal mucosa Agarose Gel Electrophoresis (AGE) Polyacrylamide Gel Electrophoresis (PAGE) SDS-PAGE ELISA RFLP Bioinformatics Introduction to Bioinformatics. Application & Research, Present Scenario Basics of C/C++ programming

Module – 2 Duration: 4 weeks Fee: Rs. 8,000/- Advanced Concepts of Biotechnology SSCP RFLP STR VNTR Advanced Molecular Biology Techniques & Applications Isolation & Separation of Nucleic Acid (DNA) from Various Sources - Plant leaves, Green gram seeds, Saliva, blood etc. Qualitative & Quantitative Analysis of DNA through Gel Electrophoresis Polyacrylamide Gel Electrophoresis (PAGE) Electrophoresis and Elution of DNA Restriction Digestion of λ DNA using molecular scissors Staining Techniques Silver staining Ethidium Bromide staining

Reagent Preparation Reagent preparation of different molarities. Reagent preparation of different concentrations. Immunology Blood group estimation PCR and its application Introduction to PCR Handling & Programming Amplification of nucleic acid (DNA) through PCR Electrophoresis of PCR Products Forensic application of PCR Clinical Application of PCR Relationship Testing through PCR DNA Fingerprinting Introduction Application in Human Identification , Relation establishment & Forensics DNA profiling through STR Analysis

Every Trainee will be provided their 16 Genetic Marker FBI CODIS standard DNA Profile on a plastic card. This DNA profile is unique for every individual on the planet and is the fool proof source of Human Identification. The test costs around Rs. 12,000/- in the market. This will be provided free of cost to all trainees who include Module-2 in their program.

Module – 3 Duration: 4 weeks Fee: Rs. 10,000/-

Introduction to Next Generation DNA Sequencing and its Applications

History of DNA Sequencing and Introduction to Next Generation DNA Sequencing

a.) Need for DNA Sequence Information b.) Principles of early DNA sequencing technologies

and its limitations c.) Next Generation DNA Sequencing or Massively

Parallel Sequencing Leading Next Generation DNA Sequencing Technologies

a.) Pyrosequencing- Principle, advantages and limitations

b.) Sequencing by Oligonucleotide Ligation and Detection (SOLiD)- Principle, advantages and limitations

c.) Sequencing by Synthesis (Reversible Dye Termination)- Principle, advantages and limitations

Sequencing by Synthesis - Principle and Detailed Workflow

a.) General workflow and Experimental design b.) NGS Library preparation for genomic DNA

sequencing c.) Cluster amplification and Sequencing d.) Understanding NGS data and analysis tenets

Application of NGS in Agriculture, Health and Environment Research

a.) Sequencing of Orphan Genomes b.) Re-sequencing for genetic marker discovery

and diagnostics c.) Transcriptome Sequencing for Gene

discovery and profiling d.) Epigenome analysis e.) Metagenome analysis from environmental

samples

Live demonstration of Next Generation Sequencing Technique using Illumina® Genome Analyzer IIx and introduction to practical workflow

Only Module – 1 can be done individually. Module-2 and Module-3 have to be paired with minimum one other module (for eg. 1 & 2 OR 1 & 3 OR 2 & 3)

Important:

Service Tax @ 10.3% applicable on total fee.

Registration Process:

1. Fill up the registration form with complete details. (Form available at http://inbit.inbdna.com/Registration.html)

2. Affix one recent passport sized colour photograph on form. 3. Attach copy of recommendation Letter / University Letter / ID Card / Voter I.D. / Any other valid ID Proof. 4. Prepare and attach Demand Draft for Rs 1,000/- in favour of "Indian Biosciences", payable at New Delhi.

(Registration Fee is non refundable and adjusted in the total fee) 5. Post the documents by reputed courier service to:

Indian Biosciences, G-2 (Ground Floor Rear) Kailash Colony, New Delhi - 110048, India.

In case of further queries, please contact us on: 0124 4256088, 09818779747 OR

Email: inbit@inbdna.com