IMS Supervisors Recruiting 2016 SURP students · 2017-07-14 · IMS Supervisors Recruiting 2016 SURP students First Name Family Name Email Anne Bassett [email protected] Office

IMS Supervisors Recruiting 2016 SURP students

First Name Family Name Email

Richard Aviv [email protected]

Office Phone # Lab Phone # Address

416-454-1997 416-480-6100 x 89617 AG31e, Sunnybrook Health

Sciences Centre

Project Title

Does the lobar distribution of cortical gray matter perfusion reduction correlate with cognitive impairment in Relapsing remitting Multiple sclerosis patients.

Project Description

Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system characterized by initial increased blood-brain barrier (BBB) permeability and perivascular lymphocyte migration. Cognitive impairment is present in 40-65% of MS patients and correlates with cortical lesion volume. Clinico-pathological correlation using high field ex-vivo MRI demonstrates limitations in prospective cortical lesion identification using proteolipid protein staining as a reference standard. Recently, studies demonstrate the potential for perfusion MRI to identify cortical abnormality, even in the absence of structural differences, suggesting perfusion is sensitive to changes not visible on routine structural imaging. Aim is to study correlations between lobar cortical gray matter perfusion and cognitive deficits in a prospectively recruited patient cohort. Regional perfusion abnormality will be correlated with specific cognitive tests within the MACFIMS MS battery. Research will be undertaken within Dr Aviv’s imaging laboratory at Sunnybrook Health Sciences Center. Advanced computer programming skills using MATLAB or Python are an essential requirement to apply.



Anne Bassett [email protected]


416-535-8501 x 32731 same

Centre for Addiction and Mental Health, 33 Russell

Street, Main Bldg, 1/F, Toronto, ON M5T 2S1

Project Title

Identifying genetic and clinical predictors for major neuropsychiatric diseases.

Project Description

There is a large genetic component to risk for developmental conditions, including those involving the heart and the brain. The identification of clinical and genetic markers for these diseases would allow earlier diagnosis and development of more effective treatment and potentially preventive strategies. We study human genetic models that significantly increase the power to identify such markers. Working at the University Health Network and Centre for Addiction and Mental Health, and with colleagues at The Centre for Applied Genomics (SickKids), our patient populations and extensive genetic and clinical data offer the opportunity to discover new pathways to fundamental disease mechanisms. Resources include next generation DNA sequencing data, comprehensive clinical and imaging data, longterm outcome data, and patient populations with schizophrenia, early-onset Parkinson disease, intellectual disability, epilepsy and other neuropsychiatric diseases, including those with specific genetic subtypes. These clinical and statistical/bioinformatics based research results have the potential to be immediately translated into clinical practice and have public health implications.



Nancy Baxter [email protected]


416-864-6060 x 77028 same

St. Michael's Hospital, 30 Bond Street, Toronto, ON M5B 1W8 (Student would be working at

250 Yonge Street)

Project Title

Outcomes of BRCA1/2 Testing in Ontario.

Project Description

As part of a retrospective study, we will link databases for women who have undergone genetic testing for the BRCA1/2 genes at major genetics centers in Ontario to administrative and cancer registry data. We will use the cohort created to evaluate the health and economic outcomes of testing for BRCA1/2 in Ontario. This model will enable evaluation of outcomes in women who have undergone genetic testing for breast cancer susceptibility. The student will assist in the creation of the cohort through chart abstraction. S/he will test chart abstraction forms and then extract information about family history of cancer at Mount Sinai and North York General Hospitals, where patient files will be retrieved. The student will enter abstracted data into a standard collection tool, and ensure completeness. S/he will also carry out other administrative tasks related to the cohort, such as data review meetings, quality insurance reports, etc., as assigned. Knowledge/experience with breast cancer, chart abstraction and/or database/registry is an asset. The student must be a self-starter, have a willingness to learn, and have strong problem-solving skills to contribute to the execution of the study.



Isabelle Boileau [email protected]


416-535-8501 x 34918 same Centre for Addiction and

Mental Health, 250 College Street

Project Title

Investigatin Neuroimmunie Response in Substance Use Disorders.

Project Description

Methamphetamine (MA) abuse is associated with significant consequences to social and personal health, but there is no approved medication for this condition. Neuroglia activation / neuroimmune signaling is increasingly recognized as novel therapeutic target(s) for MA addiction. This notion is based on animal data suggesting involvement of altered brain glial functions and neuroinflammatory factors in this condition and findings that some drugs with neuroimmune properties reduce addiction relevant behaviors (e.g. drug-seeking in rodents). Although animal studies suggest some brain glial activation following MA exposure, the data remain scanty and contradictory in the human. There is need to establish whether the brain animal findings have any relevance to the human MA user. Following upon our development of [18F]-FEPPA, a non-invasive PET imaging probe which can assess glia activation via measurement of TSPO binding, our major specific aim and hypothesis is to establish by PET imaging whether brain glial activation is above-normal in chronic MA users during early withdrawal. We will also measure TSPO (protein) in a parallel autopsied brain study and examine the relationship between glial activation ([18F]-FEPPA / TSPO PET binding), oxidative stress (glutathione [GSH] via MRS) and peripheral blood measures of immune markers and explore whether those might be related to behaviour and neural impairment characteristic of MA withdrawal. MA users will participate in a PET imaging session with [18F]-FEPPA and an MRI/MRS session to provide structural information needed for PET data analysis as well as GSH levels (MRS) and functional connectivity (rsMRI). MA users will be scanned in early abstinence to maximize chance of seeing an effect and subjective assessments of craving, mood, withdrawal and cognitive state taken. Blood will be drawn for measurement of immune markers, permitting exploration of relationship between PET/MRI-MRS outcome measure and peripheral measures. Confirmation of our hypotheses conducted by a team having a track record in both postmortem and living brain assessment of glial status, can help guide clinical application of treatment approaches targeting the neuroglial cell and possibly neuroimmune response. Project will also provide the first simultaneous imaging investigation of microglia activation and oxidative stress in MA users. Further, assessment of the relationship between brain and peripheral (blood) markers in MA addiction is highly innovative, a "first" in this condition, and will address the unmet need for identification of simple, noninvasive and inexpensive biomarkers of CNS “damage”.



Andrea Boggild [email protected]


416-340-3675

Tropical Disease Unite, Toronto General Hospital, 200 Elizabeth Street, 13EN-218, Toronto, ON

M5G 2C4

Project Title

Towards Diagnostic and Therapeutic Stewardship in Imported Tegumentary Leishmaniasis.

Project Description

Cutaneous leishmaniasis (CL) is an emerging vector-borne disease increasingly imported to North America from the tropics by travelers and migrants. Many aspects of CL pathogenesis remain unanswered, including the contribution of both bacterial and viral co-pathogens to disease severity. In addition, the determinants of Leishmania susceptibility to well-tolerated oral medications, such as fluconazole, have yet to be elucidated. The goal of this proposed study is to generate knowledge around Leishmania epidemiology and pathogenesis, and in doing so, influence evidence-based approaches and guidelines to diagnosis and treatment of cutaneous leishmaniasis (CL) in Canada. OBJECTIVES. Using ATCC Leishmania strains, and surplus biobanked clinical isolates, we aim to: 1. Understand the microbiome of CL-ulcers in both inflammatory and non-inflammatory CL; 2. Elucidate the role of the parasitic virus LRV1 in Leishmania infectivity and clinical phenotype; 3. Characterize strain susceptibility to fluconazole, and to perform surveillance for inherent and acquired resistance to anti-leishmania drugs to inform development of an "anti-leishmaniagram". In order to achieve our aims, we will use a combination of laboratory validation and analytic approaches, including cell culture, end-point and real time PCR, and pyro- and Sanger sequencing. The SURP student will contribute to all aspects of the project, including presentation of results.



Vinod Chandran [email protected]


416-603-5192 1E416, Toronto Western

Hospital, 399 Bathurst Street, Toronto, ON M5T 2S8

Project Title

Prognosis and biomarkers studies in psoriatic arthritis.

Project Description

Position 1. The student will participate in one of a number of ongoing and proposed clinical studies related to the prognosis of psoriatic arthritis. These studies include assessment of outcomes in these patients and the factors related to these outcomes. The student will collect clinical data, administer instruments of health status, collate information and participate in the analysis of the data. The student will be exposed to clinical research methodology as well as learn the clinical features, assessment and management of patients with rheumatic diseases. Position 2. The student will participate in one of a number of ongoing and proposed investigations of the role of biomarkers in susceptibility to and expression of psoriatic arthritis. The student will be learning molecular techniques of HLA typing, other genetic markers, soluble biomarkers, will be exposed to research methodology as well as learn the clinical features of patients with rheumatic diseases. Laboratory experience is required. Some knowledge of immunology, molecular biology is required.



Chung-Wai Chow [email protected]


416-340-3512 416-340-3512 or 416-581-7636 585 University Avenue, 11 PMB

130, Toronto, ON M5G 2N2

Project Title

Traffic-related air pollutants and the transplanted lung.

Project Description

The student will participate in an ongoing longitudinal research project that is focused on personal pollution monitoring in lung transplant recipients, and will be working with a multi-disciplinary team of researchers in clinical medicine and engineering. The student will help in collection and processing of patient samples, retrieval of data from clinical records, and collection and analysis of pollutant measurements. It is anticipated that the student will be able to develop a subproject during the 12 week duration of the SURP. Details of the sub-project will be discussed with the student prior to start of the SURP. Possibilities include pollution monitoring of specific public sites where transplant patients spend a lot of time, or recruitment of normal healthy subjects as a validation cohort for the lung transplant recipients or participating in a parallel project using a mouse model of lung disease.



Kim Connelly [email protected]


416-864-5201 416-864-5201 x 77635 30 Bond Street, Rm 7-052,

Bond Wing, Toronto, ON M5B 1W8

Project Title

Investigating mechanisms of cardiovascular benefits in diabetes using SGLT2.

Project Description

Diabetes is associated with increased cardiovascular mortality and morbidity. Current therapies such as thiazolidenediones have been associated with excess cardiac events. New therapies such as serum glucose co-transporter inhibitors (SGLT-2i) appear promising. However, the mechanism and safety of these compounds in the setting of heart failure and post myocardial remodeling remains unclear. We will now block the SGLT2i receptor, and determine the effect upon cardiac and renal function in diabetic animals which undergo myocardial infarction and develop heart failure.



John Coles [email protected]


416-813-6204 555 University Avenue, Toronto, ON M5G 1X8

Project Title

Ex Vivo pig model of cardiac death donation.

Project Description

Method of work: development of perfusion model ; echo & metabolic assessment. Student is in a surgery track & has experience with intact porcine models & hemodynamic assessment. Applicant will harvest porcine heart using standard CP solution & techniques similar to transplantation. Will the insert arterial and venous cannula & initiate ex vivo perfusion & conduct Echo monitoring over variable times (0-4 hrs). Objective is clinical translation of method to prolong donor heart storage.



Vincenzo De Luca [email protected]


416-535-8501 x 34421 416-535-8501 x 34409 CAMH, 250 College Street,

Toronto, ON

Project Title

Neurobiology of psychoses.

Project Description

In Canada, suicide is one of the leading causes of death for both males and females from adolescence to middle-aged adulthood. Attempted suicide is an important predictor of completed suicide and the presence of a previous suicide attempt significantly increases the risk for repeated attempt and completed suicide. Suicide is a complex behaviour in which genetics and stressful life events play a role in modulating the risk. Schizophrenia, a common psychiatric disorder, increases the risk for suicide. Here, we conduct a study using well validated genetic variants that confer risk for schizophrenia and test these variants for association with suicide attempt in schizophrenia. Childhood trauma has been consistently associated with suicidal behaviour. We plan to study a sample of 603 patients with schizophrenia, recruited from the two clinical sites in Toronto, and analyze 108 genes. The focus of the genetic analysis is to use genetic variants that directly affect the susceptibility for schizophrenia. We will be able to compare the variants in patients with suicide attempt and those without. Lifetime suicide attempt will be assessed by the means of standardized clinical tools. This proposal explores a specific hypothesis that the genetic variation directly influencing risk for schizophrenia interacting with childhood trauma can modulate suicide risk. These genetic risk markers for suicide, in the future, can identify persons at risk and thus facilitate prevention by the means of counselling, family education, and early intervention. Furthermore, the gene interaction with childhood trauma may reveal new neurobiological mechanisms in suicide that may be used as targets for development of novel treatment to reduce suicidal behaviour and in turn prevent suicide.



Gabrielle deVeber [email protected]


416-813-7721 same

The Hospital for Sick Children, 555 University Avenue, Rm

6537 Hill, Toronto, ON M5G 1X8

Project Title

Paediatric Stroke Outcome Measure (PSOM).

Project Description

The Pediatric Stroke Outcome Measure (PSOM) was developed out of a need for an objective, standardized outcome measures for children with stroke. This measure was developed at The Hospital for Sick Children and it has been shown to be a reliable and valid outcome tool that can define clinically and functionally relevant outcomes in children with stroke (Kitchen et al., 2003). To date, we have 1500+ PSOM assessments entered in a database for 500+ stroke patients. The student will analyze the data using Item Response Theory (IRT) models to define the immediate and, more importantly, long-term predictors of neurological outcome in children surviving ischemic stroke. Experience in working with psychometric tests is preferred. Research experience required. Strong attention to detail, critical thinker, and the ability to interpret data into meaningful results. Strong knowledge of statistical analyses. Able to effectively communicate, both written and verbally.



Andrea Doria [email protected]


416-813-6079 416-813-7500 x 302482 The Hospital for Sick Children,

555 University Avenue, Toronto, ON M5G 1X8

Project Title

MRI for Diagnosis of Equivocal Initial Ultrasounds in Suspected Pediatric Appendicitis: “Ultrasound First, MRI Second Approach”. A Cost-Effectiveness Analysis.

Project Description

Diagnosing pediatric acute appendicitis remains challenging. Children with suspected appendicitis are typically assessed by ultrasound (US) and/or computed tomography (CT). CT is more accurate than US, but exposes children to harmful radiation. A problem with US is the fact that many children with suspected appendicitis have equivocal initial USs, and a second US may require a long interval in order to achieve helpful results, thus increasing patient hospital stay and management costs. MRI is a radiation-free imaging modality with a diagnostic accuracy similar or higher than CT which can be performed immediately after an equivocal initial US, and holds potential for being more efficacious than other modalities for diagnosing appendicitis. In this studybased on retrospective data the successful student will estimate the incremental costs of a clinical-ultra-fast MRI pathway (new strategy) as compared with a clinical serial US pathway (standard care strategy) when the initial US is equivocal, per case of false-negative or false-positive or perforated appendicitis averted. If MRI proves to be more effective in ruling in/ruling out disease than US, and if it is equally or less costly than US the results of this study may have a significant impact on optimization of diagnostic imaging in pediatric appendicitis, refining the utilization of financial resources of the health care system. Student’s background in imaging is desirable, but not mandatory.



Yigal Dror [email protected]


416-813-5886 416-813-4200

Genetics and Genome Biology Program, Research Institute, The Hospital for Sick Children

Peter Gilgan Centre for Research and Learning, 14th

floor, Room 14-9704, 686 Bay Street, Toronto, ON M5G 0A4

Project Title

The student will take part in one of the following two projects: 1. Dissecting the underlying hematopoietic defects in bone marrow failure disorders using induced pluripotent stem cells. 2. Functional analysis of novel inherited.

Project Description

The students will join a PhD student who work on one of the following projects and will perform a part of the study. Project 1: in this project we use disease associated- induced pluripotent stem cells (iPSCs) from healthy control iPSCs. We characterize the stem cells and their differentiation potential and dissect the role of the associated gene in normal homeostasis of the hematopoietic system. The ultimate aim is to identify therapeutic biochemical targets and candidate novel drugs. Project 2: Through genome wide approaches and whole exome sequencing, we have identified several candidate bone marrow failure genes. In this project along with continuing the search for more genes and more affected patients, we perform functional assay and rescue experiments to confirm the gene role in the disease. After confirmation, disease models with iPSCs are generated and studied.



I. George Fantus [email protected]


416-586-8665 416-581-7680 Mount Sinai Hospital, 60 Muray

Street, Room 5028, Toronto, ON M5T 3L9

Project Title

Role of thioredoxin-interacting protein (TxNIP) in diabetic cardiomyopathy.

Project Description

Thioredoxin-interacting protein (TxNIP) is an alpha-arrestin domain containing protein which binds to and inhibits thioredoxin (Trx), an endogenous antioxidant oxidoreductase. This interaction promotes oxidative stress, cellular dysfunction and apoptosis. TxNIP is markedly upregulated by high glucose in various cell types including cardiomyocytes. Our lab has demonstrated that TxNIP promotes oxidative stress not only by inhibiting Trx antioxidant function but also by increasing mitochondrial glucose oxidation (J. Biol. Chem. 2013). We have also demonstrated that TxNIP-/- (KO) mice are protected from diabetic nephropathy (J. Am. Soc. Nephrol. 2015). We hypothesize that TxNIP KO mice will also be protected from diabetic cardiomyopathy. This project will include experiments in cultured cardiomyocytes exposed to high glucose to determine whether TxNIP is involved in altered gene expression and cellular function observed in diabetes/high glucose. Subsequently,cardiac-specific TxNIP KO mice will be made diabetic with streptozotocin to determine if TxNIP-deficiency is protective in vivo. Functional studies using echocardiography will be performed as well as post-mortem pathological assessment and alterations in gene expression determined in control and diabetic WT and cardiac specific TxNIP deficient mice.



Michael Fehlings [email protected]


416-603-5085 416-603-5229

Krembil Discovery Tower, Toronto Western Hospital, 60

Leonard Avenue, 7KD430, Toronto, ON M5T 2S8

Project Title

Repair and regeneration of the injured brain and spinal cord.

Project Description

The Fehlings Laboratory is using clinically relevant murine and rodent models of brain and spinal cord injury to understand the pathophysiology and treatment of CNS injury. The strategies being examined include neuroprotective approaches to attenuate programmed cell death, bioengineered strategies to bridge regions of CNS disconnection and neural stem cell-based regenerative strategies. The techniques used incorporate the full breadth of cutting edge molecular, imaging, behavioural and functional analyses. The student will also be exposed to an exciting translationally based clinical research program in which laboratory discoveries are moved into the clinical sphere. Research interests: 1) Neuroprotection of the injured brain and spinal cord 2) Regenerative medicine for brain and spinal cord injury using stem cell and tissue engineering 3) Mechanisms of glial-axonal signaling using electrophysiological and imaging approaches 4) Clinical trials Students in the higher years (e.g. 3rd or 4th year) are preferred.



Andrew / Neil Feifer / Fleshner [email protected]


905-997-2805 2300 Eglinton Avenue West, Suite 411, Mississauga, ON

L5M 2V8

Project Title

Contemporary Management of Patients with Castrate Resistant Prostate Cancer in a Dedicated Clinic: Impact of Care Centralization on Patient Quality of Care and Health Care Cost Expendtiture.

Project Description

The student will be centrally involved in undertaking a project to evaluate the impact of the development of the Trillium Health Partners Center fro Advanced and Metastatic Prostate Cancer. We intend to amass data from a busy community academic affiliated hospital in the CRPC clinic and define disease specific, health related quality of life, and cost effectiveness outcomes. The student will have a great opportunity to work in a multidisciplinary environment, with world-class uro-pathology staff, urologic oncology and health outcomes scientists. The student will be given an opportunity to develop ideas and contribute to hypothesis generation, as well as data structure management, and will learn the fine nuances of data analysis, multivariable analysis and other important large database management and evaluation strategies. No statistical experience required some basic familiarity with Microsoft excel suggested. This is a great opportunity that will lead to the publication of a number of academic publications, on which the student’s contribution will be appropriately reflected.






L5M 2V8

Project Title

Cost Analysis and Quality of Life Impact of the Introduction of Minimally Invasive Renal Surgery for Renal Cell Carcinoma.

Project Description

The student will be centrally involved in undertaking a project to evaluate the impact of surgical innovation for renal cell carcinoma. Kidney cancer is more and more prevalent, and the introduction of laparoscopy and minimally invasive surgical innovation has dramatically changed the wa7 in which the patient’s experience their disease treatment. We intend to amass data from a busy community academic affiliated hospital group regardin the surgical treatment of men with localized renal cell carcinoma, and to evaluate the cost impact to our institution, to overall health care expenditure, and the patient satisfaction and quality of life. The student will have a great opportunity to work in a multidisciplinary environment, with world-class uro-pathology staff, urologic oncology and health outcomes scientists. The student will be given an opportunity to develop ideas and contribute to hypothesis generation, as well as data structure management, and will learn the fine nuances of data analysis, multivariable analysis and other important large database management and evaluation strategies. No statistical experience required some basic familiarity with Microsoft excel suggested. This is a great opportunity that will lead to the publication of a number of academic publications, on which the student’s contribution will be appropriately reflected.






L5M 2V8

Project Title

The Impact of percent Gleason Pattern 4 and 5 on Contemporary Outcomes of Surgically Treated Men for Prostate Cancer.

Project Description

The student will be centrally involved in undertaking a project to discover the impact of Gleason 4-5 prostate cancer on long term biochemical free and overall survival in contemporarily treated men. The student will have a great opportunity to work in a multidisciplinary environment, with world-class uro-pathology staff, urologic oncology and health outcomes scientists. The student will be given an opportunity to develop ideas and contribute to hypothesis generation, as well as data structure management, and will learn the fine nuances of data analysis, multivariable analysis and other important large database management and evaluation strategies. No statistical experience required some basic familiarity with Microsoft excel suggested. This is a great opportunity that will lead to the publication of a number of academic publications, on which the student’s contribution will be appropriately reflected.



Thomas Forbes [email protected]


416-340-3274 Toronto General Hospital, 200 Elizabeth St., Eaton North 6-222, Toronto, ON M5G 2C4

Project Title

Experimental investigation of the causes of rotation of stent grafts used to treat abdominal aortic aneurysms.

Project Description

Fenestrated aortic stent grafts are medical devices used to treat abdominal aortic aneurysms. The objective of our project is to experimentally determine the causes of rotation of these devices upon deployment, which is hypothesized to occur due to an accumulation of rotational energy as the device is delivered through the iliac artery. To do so, we have designed an experimental apparatus to deliver and deploy these devices in idealized and patient-specific geometries and measure the resulting rotation. The student will work with a graduate student on all aspects of our experiments including, but not limited to, medical image segmentation, design of idealized and patient-specific anatomical phantoms in SolidWorks for 3-D printing, delivery and deployment of stent grafts in these phantoms, and measurement of coefficients of friction between the stent graft, its delivery system, and the surrounding vessels. A background in engineering is an asset, but not a requirement, as is experience with SolidWorks software.



Lucia Gagliese [email protected]


416-340-4800 x 6699 same

Princess Margaret Cancer Centre, Department of

Supportive Care, Research Division, LuCliff Place, Suite

2300, 700 Bay Street, Toronto, ON M5G 1Z6

Project Title

Cancer pain in older people with delirium: Healthcare workers’ perspectives.

Project Description

Many older people with cancer experience pain and delirium at the end of life. This has been associated with distress for both patients and family members and may contribute to poorer quality of death and dying. We do not have a valid way to measure pain in these vulnerable patients. The overall aim of this research program is to develop a pain assessment tool for these patients. In order to do so, it is important first to understand healthcare workers’ experiences. In this study, healthcare workers were interviewed to obtain their perspectives on pain and delirium in older people with cancer. The objective of the student’s project will be to analyze these interviews to identify the challenges faced by healthcare workers. The student’s responsibilities will include literature review and learning about the relevant topics, data analysis, and preparation of a written report, abstract and poster. The student will participate in the lab’s ongoing activities, as appropriate. Previous research experience is an asset but not required. To learn more about our lab, please visit Cancer Pain Lab (http://cancerpain.lab.yorku.ca)



J. Ted Gerstle [email protected]


416-813-6401 416-813-7654 x 204652

Hospital for Sick Children, Roy Hill Wing, Room 1526C, 555

University Avenue, Toronto, ON M5G 1X8

Project Title

Magnetic resonance-guided high intensity focused ultrasound for High-risk Neuroblastoma.

Project Description

Despite multimodality therapy, the overall survival rate for high-risk neuroblastoma (HR-NBL) is less than 40%. A novel treatment option for HR-NBL is magnetic resonance-guided high intensity focused ultrasound (MR-HIFU): a specially designed transducer is used to focus a beam of ultrasound energy at a specific target site in the body through the skin without requiring an incision. The ultrasound energy produces sufficiently high temperatures in the target tissue to cause cell death. The objectives of this recently-funded study are to evaluate the overall safety and efficacy of MR-HIFU in patients with HR-NBL. The study will be divided into two parts: Part I will involve obtaining pre-clinical data with a xenograft mouse model of NBL and a pig model to establish HIFU safety and therapy parameters for HR-NBL. Part II will be a Phase I clinical pilot study. HR-NBL patients who have refractory or relapsed disease will be recruited and the tumors will be targeted with MR-HIFU. The student’s role would be to work with the PI and the research team on a component of the project, which would be determined based on the initial results of the study, which is starting in January 2015. No specific experience required.



Dafna Gladman [email protected]


416-603-5753 1E410B, Toronto Western


Project Title

Prognosis and biomarkers studies in psoriatic arthritis and systemic lupus.

Project Description

Postition 1. The student will participate in one of a number of ongoing and proposed studies related to the prognosis of rheumatic diseases including psoriatic arthritis, and systemic lupus erythematosus. These studies include assessment of outcomes in these patients and the factors related to these outcomes. The student will collect clinical data, administer instruments of health status, collate information and participate in the analysis of the data. The student will be exposed to clinical research methodology as well as learn the clinical features, assessment and management of patients with rheumatic diseases. Position 2. The student will participate in one of a number of ongoing and proposed investigations of the role of biomarkers in susceptibility to and expression of rheumatic diseases, including systemic lupus erythematosus, psoriatic arthritis. The student will be learning molecular techniques of HLA typing, other genetic markers, soluble biomarkers, will be exposed to research methodology as well as learn the clinical features of patients with rheumatic diseases. Laboratory experience is required. Some knowledge of immunology, molecular biology is required.





416-603-5800 x 5753 416-603-5800 x 5123 399 Bathurst Street, Toronto,

ON M5T 2S8

Project Title

Profiling of transcriptomic biomarkers of psoriatic arthritis in leukocyte cell subsets.

Project Description

Psoriatic arthritis (PsA) is an immune-mediated disease that is associated with psoriasis, an inflammatory skin condition. Biomarkers that aid in the diagnosis and detection of PsA in patients with psoriasis are lacking. We completed microarray profiling of peripheral blood and identified four candidate biomarkers (CXCL10, HAT1, SETD2 and NOTCH2NL) that were differentially regulated between patients with PsA and psoriasis only. The aim of the summer research project is to characterize the expression of these biomarkers in cell subsets from peripheral blood of patients with PsA and psoriasis. The summer student will purify cell subsets (T cells, NK cells, and monocytes) from peripheral blood mononuclear cells and use real-time PCR to assess the expression of CXCL10, HAT1, SETD2 and NOTCH2NL. The student will also complete statistical analysis to analyze gene expression while taking into account the demographic and disease characteristics of patients. The summer student should have good experience with pipetting due to the precision that is required for these experiments.





416-603-5753 same Toronto Western Hospital, 399

Bathurst Street, 1E-410B, Toronto, ON M5T 2S8

Project Title

Psoriatic Arthritis, Systemic Lupus Erythematosus.

Project Description

Research into prognosis of psoriatic arthritis and systemic lupus erythematosus includes identifying factors which are associated with poor outcome, as well as those associated with remission. Patients have been followed prospectively for about 40 years and data are collected in a computerized database. Students are encouraged to develop their own research questions and design their research project together with the supervisor. There is support from research assistants and a biostatistical team.



Sergio Grinstein [email protected]


416-813-5727 416-813-5729 Hospital for Sick Children, 555


Project Title

Phagocytosis and mechanisms of bacterial killing by macrophages.

Project Description

Macrophages and neutrophils are the first line of defense of the immune system. These cells engulf invading microorganisms and other particulate material, and proceed to kill and degrade pathogens. Killing and degradation occur inside a vacuole, called phagosome, that undergoes striking biochemical changes in order to acquire microbicidal activity. We are studying the molecular basis of phagosome remodeling (maturation) in intact cells, using genetically-encoded fluorescent biosensors in combination advanced digital imaging. The student will be involved in all aspects of the analysis of the role of phosphoinositides in phagosome maturation.



Jill Hamilton [email protected]


416-813-7654 x 205115 555 University Avenue, Rm

5447B, Toronto, ON M5G 1X8

Project Title

An opportunity to reach obese adolescents and provide care locally: an evaluation of a multi-disciplinary videoconferencing approach.

Project Description

Telehealth services linking health care professionals and patients through videoconferencing have expanded in Ontario to provide services in many more locations, with recent addition of in-home videoconferencing through secure portals. These services aim to enhance access to care, reduce wait-times, travel times and cost to patients. The SickKids Team Obesity Management Program, an interdisciplinary health team, has initiated the use of Ontario Telemedicine Network (OTN) to connect with patients through this forum. This has enabled our team to replace some face-to-face education/counselling sessions with OTN videoconferencing. Outcomes including staff and patient characteristics and health changes (e.g. BMI), frequency of contact has not yet been evaluated to date. We hypothesize that use of OTN will result in increased contact with team for patients attending STMOP outside of GTA, fewer missed appointments, reduced BMI and improved health care satisfaction (a measure collected in existing program). The student’s responsibilities will include: 1. Completion of a literature review related to videoconferencing use and pediatric obesity 2. Participate in data collection and analysis with team members 3. Contribute to dissemination of findings (eg poster, manuscript) Student’s background/experience required: reliable, enthusiastic, interest in pediatric obesity/quality improvement. Data management/analysis experience is preferred.



Nigil Haroon [email protected]


416-603-5634 416-603-5456 399 Bathurst Street, 1EW-425,

Toronto, ON M5T 2S8

Project Title

Impact of smoking on disease manifestations of ankylosing spondylitis.

Project Description

Ankylosing spondylitis (AS) is a chronic inflammatory arthritis that affects about 0.5-1.0% of the general population. The disease starts during early adulthood and manifests as pain and stiffness in the lower back. As the disease progresses, new bone formation is seen at inflammatory sites resulting in spinal fusion and severe loss of mobility. Recently smoking was identified as an important risk factor for spinal fusion in AS. This study takes advantage of the large clinical research database at the Toronto Western Hospital with information on disease and treatment variables of over 1300 AS patients followed over 10 years. The impact of smoking on disease activity, extra articular manifestations and treatment responses will be assessed.



Elise Heon [email protected]


416-813-8606 416-813-7654 x 301510

The Hospital for Sick Children, PGCRL (Peter Gilgan Centre for

Research and Learning), Genetics and Genome Biology, 686 Bay Street, Rm 14-9400,

Toronto, ON M5G 0A4

Project Title

Mutation analysis of inherited eye disorders using nex-generation sequencing tools.

Project Description

This project would involve the bioinformatic assessment of whole exome/genome/RNAseq analysis for pathogenic variant candidates, and the subsequent validation by PCR, Sanger Sequence, Restriction enzyme digest, family segregation. Candidates should have academics in genetics, some experience with public databases (eg. NCBI,OMIM,UCSC Genome Browser), experience with primer design and PCR.



Paul Hwang [email protected]


416-224-1922 416-224-5527 North York General Hospital, 1110 Sheppard Avenue East,

Suite 402, North York, ON

Project Title

Perampanel, an AMPA-receptor antagonist anticonvulsant, for partial-onset and generalized epilepsies.

Project Description

Perampanel is a unique anticonvulsant that acts at the AMPA-receptor, recently released for the treatment of refractory epilepsy with partial-onset seizures, now available for primary generalised epilepsy as well (Fycompa-TM, Eisai Canada). The proposed case-cohort study compares the outcome of treatment with perampanel in 2 groups: partial onset and generalized epilepsy refractory to standard AEDs: clinical seizure control, EEG changes, interactions with other AEDs, plasma levels and quality of life. The student should have had neuroscience background, some pharmacology of AEDs, database analysis and statistical methods, and be willing to perform limited review of clinical records, develop spreadsheets of data and analyse them by database such as MS-Access, using T-tests, Chi-square, etc. An oral presentation to the University of Toronto Epilepsy Research Program is expected, and a poster at the SURP annual day of the IMS, as well as attendance at seminars, EEG and sleep lab sessions.



Harry Janssen [email protected]

applications to be sent to: [email protected]


416-603-5986 416-581-8628

Project Title

Immunology of Chronic Hepatitis B Infection.

Project Description

Our projects are focused on understanding the virus and host interaction in the context of Chronic HBV infection. We use cell culture systems and patient samples to address our scientific questions. The students are expected to have a good biology background, basic knowledge of immunology, basic understanding of laboratory techniques, and have been immunized against or be open to becoming vaccinated against HBV.



Marc Jeschke [email protected]


416-480-6703

Sunnybrook Health Sciences Centre, 2075 Bayview Ave., Room D7 04, Toronto, ON

M4N 3M5

Project Title

Uncovering the role of mitochondria in postburn hypermetabolism: Therapeutic cues to alleviate patient morbidity.

Project Description

The proposed research project will aim to further unearth the role played by the mitochondrion in postburn hypermetabolism, a ubiquitous response to severe burn injury and major contributor to patient morbidity and mortality. This fundamental organelle plays key parts in cellular bioenergetics, including ATP production, antioxidant synthesis and lipid homeostasis. A clear picture of how these functions are modulated following burn injury may inform the development of therapies aimed at improving patient outcome via a reduction in the hypermetabolic response. The ideal student will have a background in basic biochemistry. Experience with pipetting, preparing buffers, electrophoresis, cell culturing and data analysis are assets. The student will work closely with Dr. Christopher Auger to extract mitochondria from adipose tissue, with which various enzymatic assays and metabolite quantifications will be performed. At the conclusion of the research program, the student is expected to have a familiarity with several biochemical assays, and a strong understanding of the translational research approach of the work environment.



Marc / Saeid Jeschke/ Amini-Nik [email protected];

saeid. [email protected]


416-480-6100 x 85433 416-480-6100 x 88054 M7-161, Lab: M7-140, 2075

Bayview Avenue, Toronto, ON M4N 3M5

Project Title

Characterizing regenerative capacity of liver post thermal injury: liver stem cells in action.

Project Description

Severe burn injury results in local and systemic responses including profound hepatic alterations. We are exploring how hepatic progenitor cells affect liver regeneration in injury models like burns. Using different in vitro and in vivo techniques, we characterize the fate of hepatic progenitor cell post thermal injury. The lesson learned from this project will not only provide insights in liver reaction after injury but also highlight the mechanism(s) that might lead to liver regeneration. Applicant should be familiar and comfortable with cell culturing, western blotting and immunohistochemistry. Being familiar with confocal imaging and flow cytometry analysis is ideal.



Yaping Jin [email protected]


416-978-7938 340 College Street, Suite 400,

Toronto, ON M5T 3A9

Project Title

Trends and prevalence of self-reported cataracts in Canada.

Project Description

A cataract is a clouding of the lens in the eye. It is the principal cause of blindness in the world. A simple surgery, i.e. cataract surgery, will enable the patient to regain clear vision. However, it is unknown in Canada how many people are affected by cataracts and are awaiting for cataract surgery. This information is critically important for healthcare planning and healthcare resource allocation. When needed, Canadian researchers have to extrapolate similar data from other countries such as the USA. Given the different healthcare systems in USA and Canada, the validity of using the American data to guide Canadian healthcare planning is questionable. The aim of this study is to provide population estimates and analyze trends in the self-reported prevalence of cataracts in Canada and compare these to published trends elsewhere. We will use data collected from the National Population Health Survey and the Canadian Community Health Survey and perform literature search to answer our study questions. The study results will provide for the first time the number of Canadians affected by cataracts and reveal associated trends. The comparisons with data from other countries will uncover the similarities and differences in cataracts between Canada and other countries.



Jennifer Jones [email protected]


416-581-8603 200 Elizabeth Street, Munk B-045, Toronto, ON M5G 2C4

Project Title

Cander Survivorship.

Project Description

Despite the increasing incidence of cancer, mortality rates have dropped significantly over the past three decades. For the majority, cancer can now be viewed as a curable or chronic disease and as advances continue in cancer control, the numbers of people surviving cancer will continue to rise. In response, there has been a recent surge of attention paid to the field of cancer survivorship leading to efforts to manage treatment related sequelae, enhance quality of life and improve the overall functioning of people who are receiving long-term follow-up care after cancer treatment. The Health, Wellness and Cancer Survivorship Centre (ELLICSR) opened at University Health Network (UHN) is a 12,000 sq ft research facility that houses researchers and self-management research facilities and offers the opportunity to examine new approaches to predict, prevent and manage long-term adverse effects of cancer and its treatment. See http://www.ellicsr.ca for more information on our Centre. IMS SURP student will have the opportunity to participate in one of a number of ongoing studies related to the detection, prevention and treatment of cancer treatment related sequelae. The goal is to provide the student with a broad research training experience with the direct support of the experienced research team and supervisor.



Sheena Josselyn [email protected]


416-813-7654 x 301824 same Hospital for Sick Children, 555 University Ave., Toronto, ON

M5G 1X8

Project Title

Making memories in mice.

Project Description

My lab is interested in understanding how the brain encodes and stores information. This project examines how different memories are stored using mouse models.



Rupert Kaul [email protected]


416-978-8607 416-946-7054 1 King's College Circle, MSB Rm

6356, Toronto, ON

Project Title

Probiotics and gut immunology in HIV infection.

Project Description

We are running two randomized clinical trials of probiotics to improve health outcomes in HIV-infected people from Toronto: the two trials are providing probiotics to individuals (a) as they start antiviral therapy, and (b) when they have poor immune recovery despite being on antiviral therapy for a while. The student will help with sub-studies nested within this trial to define the impact of probiotics on the level of certain cytokines and other immune parameters.



Shaf Keshavjee [email protected]


416-340-4010 same

MaRS Centre, Toronto Medical Discovery Tower, 2nd Floor, Rm

2-816, 101 College Street, Toronto, ON M5G 1L7

Project Title

Advanced diagnosis and repair of donor lungs for transplantation.

Project Description

The student will undertake research in lung transplantation, specifically assisting in studies examining the advanced diagnosis of various lung conditions prior to and after transplant. Importantly, we are investigating effective ways in which a donor lung can be repaired before surgery. Trainees will gain valuable experience observing surgeries, learning about study design, and performing data collection, analysis and manuscript writing. Trainees will benefit through experienced mentorship regarding the use of new technological innovations for lung transplantation, an opportunity that is unmatched elsewhere as we are world leaders in clinical lung transplant innovation. Trainees will also be provided an opportunity to present their own work and findings, and establish relationships with other researchers; our department investigators will serve as knowledge experts for trainees to contact freely. A background in medical science or engineering is preferred but not required.



Marianne Koritzinsky [email protected]


416-581-7841 Princess Margaret Research Tower, 12-311, 101 College

Sreet, Toronto, ON M5G 1L7

Project Title

The role of PRDX4 as a novel cancer therapy target.

Project Description

Despite recent advances in cancer diagnosis and treatment, there is still an urgent need to find new specific targets for novel therapies. One of the approaches is to identify genes that are functionally important in cancer. Our data from genome-wide screens suggest that Peroxiredoxin 4 (PRDX4) is a promising target in multiple cancer types. PRDX4 is an endoplasmic reticulum-localized peroxiredoxin, responsible for reducing intracellular levels of reactive oxygen species (ROS), such as hydrogen peroxide, protecting the cellular redox state. This makes the PRDX4 a potential attractive novel target for cancer treatment. In this project, we will assess the potential of targeting PRDX4 in pancreatic cancer using a genetic approach in vitro. We will use stable isogenic cell models expressing shRNA against PRDX4, and assess cell death (microscopy, flow cytometry), cell signalling (western blot, qPCR) and ROS (flow cytometry). This project will establish the potential for targeting PRDX4 in pancreatic cancer, and form the basis for further testing in vivo and drug development.



Mathieu Lemaire [email protected]


416-813-7654 x 309419

The Hospital for Sick Children, Peter Gilgan Centre for

Research and Learning, 686 Bay Street, 19-9704, Toronto, ON

M5G 04A

Project Title

Pathophysiology of DGKE-associated atypical hemolytic-uremic syndrome (aHUS).

Project Description

Our lab has two main "arms". First, we have expertise in doing gene discovery using whole exome sequencing focusing on patients with rare pediatric kidney diseases. Second, we then perform functional analyses in cell and animal models to figure out how/why the novel genes identified through our genomic studies cause diseases. Right now, our efforts are channeled into delineating the pathophysiological processes that lead to the formation of blood clots in small vessels of the kidneys of infants that have mutations in a gene named DGKE. This condition, atypical hemolytic-uremic syndrome, is serious for patients because most develop renal failure. A better understanding of the disease should help identify potential treatments - currently there are none.



Clifford Librach [email protected]


416-323-7727 416-323-7727 x 2214 790 Bay Street, Suite 412,

Toronto, ON

Project Title

Investigating the cellular mechanisms mediating the regenerative effects of human umbilical cord perivascular cells on the germ cell niche in rodent models of chemotherapy-induced gonadal damage.

Project Description

We have previously reported the characterization of first trimester and term human umbilical cord perivascular cells (HUCPVCs) and their regenerative properties (Hong et al. 2013). We have observed that HUCPVCs injected directly in the testis of an adult mouse model of chemically-induced testicular damage promote germ cell regeneration. The cellular mechanisms mediating HUCPVC-based regeneration in the testicular niche are not currently well understood. We hypothesize that HUCPVC secrete regenerative factors that promote survival of a functional niche, including multiple supportive somatic cell types as well as spermatogonial stem cells, immediately after gonadotoxic chemical insult. The student will conduct experiments to determine whether HUCPVC treatment leads to increased survival and proliferation of i. spermatogonia and spermatogonial stem cells and/or ii. somatic cell populations including Sertoli cells, Leydig cells, peritubular cells and vascular cells at various timepoints after injury, using immunohistochemistry for cell lineage-specific antigens and markers of apoptosis (TUNEL, cleaved-caspase 3) and proliferation (PCNA, ki67). Results will be quantified using confocal microscopy. We will determine the expression of candidate growth factors produced by HUCPVCs in this in vivo model using QCPR arrays or targeted RNAseq, as well as FISH. Since HUCPVCs have pericyte properties and interact with vasculature in their endogenous niche, we will examine and compare the integrity of the vasculature in animals that did not receive chemotherapy to those who received chemotherapy with and without cell treatment. This will be done using transmission electron microscopy and vascular permeability assays. Immunohistochemistry, high resolution scanning microscopy and image processing will be used to quantify overall tissue vascularization in each experimental group.



Christoph Licht [email protected]


416-813-7654 x 309343

The Hospital for Sick Children, Peter Gilgan Centre for

Research and Learning, 686 Bay Street, 19-9713, Toronto, ON

M5G 04A

Project Title

Unraveling pathomechanisms of atypical hemolytic uremic syndrome.

Project Description

Atypical hemolytic uremic syndrome (aHUS) is a devastating form of thrombotic microangiopathy that can be both a life and organ threatening condition. Predisposing mutations in several complement-associated genes, primarily coding for humoural and surface-bound components of the complement alternative activation pathway have been identified. The association of complement-associated predisposing mutations with aHUS has contributed to major advances in diagnosis and treatment. Mutations have been identified in about half of aHUS patients tested so far, and the disease manifests in about half of the carriers. It is likely that most mutations have already been identified and that most of our patients have been tested for them, hence the remaining likely physiological sources for gene variants relevant to aHUS onset and progression are other thrombosis-associated systems (e.g. coagulation and inflammation) and the vascular endothelium. The aim of this project is to identify aHUS-specific endothelial cell characteristics using blood outgrowth endothelial cell (BOEC) cultures. aHUS pathology involves complement, blood coagulation, infective/inflammatory triggers and the vascular endothelium. We hypothesize that laboratory models can be used to simulate aspects of aHUS pathology by bringing together the humoural and cellular components of the complement, coagulation, inflammatory and endothelial systems, and that these model systems can be used to compare the susceptibility of vascular endothelial cells cultured from aHUS patients and unaffected relatives carrying the same complement mutations.



Fang Liu [email protected]


416-979-4659 416-535-8501 x 9657 Centre for Addiction and

Mental Health, 250 College Street, Toronto, ON M5T 1R8

Project Title

Investigating the neurodevelopmental effects of disrupting D2R-DISC1 interaction in the Disc1-L100P mutant mice.

Project Description

We have previously identified a novel protein interaction between the dopamine D2 receptor (D2R) and Disrupted-in-Schizophrenia 1 (DISC1, a risk factor for schizophrenia), that is enhanced in schizophrenia patients and a mouse model of schizophrenia (Disc1-L100P). In this project, we plan to examine the neurodevelopmental effects of disrupting the D2R-DISC1 interaction using a specific peptide. The student will be responsible for experiments such as brain slicing and immunohistochemistry, confocal microscopy of fluorescence imaging, and analyzing different histological parameters. Previous experience in immunofluorescence and confocal microscopy would be an advantage.



Mingyao Liu [email protected]


416-581-7501 101 College Street, TMDT-2-814, Toronto, ON M5G 1L7

Project Title

Molecular mechanisms of acute lung injury.

Project Description

To determine the cellular and molecular mechanisms of acute lung injury. To develop nanotechnology for drug delivery to treat lung injury.



R. Loch Macdonald [email protected]


416-864-5452 416-847-1739 30 Bond Street, Toronto, ON

M5B 1W8

Project Title

Pathogenesis of Brain Injury after Subarachnoid Hemorrhage.

Project Description

The goals of our laboratory are to define the cellular and molecular mechanisms that cause angiographic vasospasm, microvascular injury and poor outcome after subarachnoid hemorrhage (SAH). The approach is translational and uses techniques ranging from animal surgery, molecular biology, immunohistochemistry to confocal imaging, microscopy and electrophysiology and focuses on mechanisms of brain injury after SAH. We are studying how blood clot contracts cerebral arteries and how to prevent it. Using variety animal models, we found that voltage gated calcium channels and endothelin-mediated signaling, possibly involving TRP proteins, are important. The role of inflammatory cytokines such as TNFa is also being studied in mouse models. We also study role of ion channels in mediating vasospasm and vascular remodeling. We developed anterior circulation SAH models in rats and mice, characterized neurobehavioral deficits in these animals and are examining mechanisms of dysfunction by electrophysiologic study of hippocampal function and molecular analysis of the brain. The student is expected to learn the laboratory methods involved in the laboratory, such as immunohistochemistry, Western blotting, electrophysiology, metaanalysis skills and such. The student must learn scientific study design, statistics and writing of a research manuscript, presenting research results at local academic meetings and national or international conferences.



David Malkin [email protected]


416-813-5348 416-813-8206 Hospital for Sick Children, 555


Project Title

Molecular determinants of cancer susceptibility: the Li-Fraumeni Syndrome paradigm.

Project Description

Li-Fraumeni syndrome (LFS) is a cancer predisposition syndrome associated with multiple, early onset cancers in both children and adults. Germline mutations of the TP53 tumor suppressor gene are responsible for ~70% of cases. The phenotypic heterogeneity of the disease (both within and across families) suggests that other genetic/epigenetic modifiers play roles in the specific patterns and occurrences of cancer. Our lab is using a variety of approaches including next generation sequencing, RNA seq, animal (mouse/zebrafish) modeling to address this question. The student will work closely with a post-doctoral fellow or graduate student to explore the relationship of germline genotypes to predicting cancer phenotype. Technical skills learned will include cell culture work, PCR and qPCR, functional assays of tumor suppressor activity, and fundamental bioinformatic approaches. The student will also participate in weekly lab meetings, journal clubs and presentations.



Helen McNeill [email protected]


416-586-8267 Room 881 LTRI, Mount Sinai

Hospital, 600 University Avenue, Toronto, ON

Project Title

Analysis of Fat cadherins in patterning and growth control.

Project Description

Fat cadherins are large cell-cell adhesion molecules, which are responsible for integrating growth and planar cell polarity (PCP), a tissue organization pathway. Fat cadherins regulate growth via the Hippo kinase pathway. PCP is crucial to proper tissue function; its loss is responsible for many human diseases, including neural tube closure and cystic kidney disease. We use Drosophila as a genetically tractable organism to investigate the developmental control of tissue organization and growth. We also use the simple organism Hydra to study the evolutionary regulation of these processes, and how Fat cadherins affect tissue regeneration. The student will study the role of Fat cadherins and the Hippo pathway in patterning and growth control. Two project options are available: 1) Generating targeted mutations in Fat in Drosophila models using CRISPR, and analyzing their effect on tissue growth and patterning or 2) Studying the role of Fat and the Hippo pathway in hydra growth and regeneration. Students should be familiar with basic molecular biology approaches and genetic analysis. Cell biology, developmental and/or genetics courses completion is helpful.



Laurent Milot [email protected]


416-480-6100 x 7418 Sunnybrook Health Sciences Centre, 2075 Bayview Ave.,

Toronto, ON M4N 3M5

Project Title

Optimization of timing of delayed phase gadolinium-enhanced MRI of colorectal liver metastases as a predictor of long-term survival in a surgical population.

Project Description

Colorectal cancer is the 2nd leading cause of cancer deaths in Canada. Half of patients will develop liver metastases and most deaths are related to metastatic disease. Untreated, the median survival of colorectal liver metastases (CRLM) is approximately 9 months. With recent advances in surgery, the median survival CRLM now approaches 5 years with up to 20% cured. However, there remains a large variability in patient prognoses and being able to accurately predict which patients will most benefit from aggressive surgeries preoperatively is still difficult. We have recently discovered that delayed enhancement on preoperative gadolinium-enhanced MRI typically used for diagnosis and staging may predict long-term survival. This will be a retrospective study is to optimize the timing at which MRI may provide the most useful information. Educational objectives for the student include learning about: (1) colorectal cancer and liver metastases, (2) MRI and the appearance of focal liver lesions on MRI, (3) liver anatomy. The student’s role in the project will include image analysis and statistical analysis. The student will also have the opportunity to participate in multidisciplinary tumour boards and lab meetings. No specific background or experience is required.



Laurent Milot [email protected]


416-480-6100 x 7418 Sunnybrook Health Sciences Centre, 2075 Bayview Ave.,

Toronto, ON M4N 3M5

Project Title

MRI segmentation of colorectal liver metastases in a nonsurgical population as a predictor of long-term survival.

Project Description

Colorectal cancer is the 2nd leading cause of cancer deaths in Canada. Half of patients will develop liver metastases and most deaths are related to metastatic disease. Untreated, the median survival of colorectal liver metastases (CRLM) is approximately 9 months. With recent advances in chemotherapy, the median survival CRLM is approximately 2 years, even among those who are not surgical candidates. We have preliminary data that suggests that several features on different MRI sequences typically used for diagnosis and staging may predict long-term survival. This study will be a retrospective study looking at the effect of these features in a nonsurgical population, with subanalyses on their response to chemotherapy treatment. Educational objectives for the student include learning about: (1) colorectal cancer and liver metastases, (2) MRI and the appearance of focal liver lesions on MRI, (3) liver anatomy. The student’s role in the project will include image analysis and statistical analysis. The student will also have the opportunity to participate in multidisciplinary tumour boards and lab meetings. No specific background or experience is required.



Berge Minassian [email protected]


416-813-7721 Hospital for Sick Children, 555 University Ave., Toronto, ON

M5G 1X8

Project Title

Pathogenesis and therapy of a rare fatal form of epilepsy.

Project Description

Our lab is dedicated to uncovering the pathogenesis of a rare but severe form of epilepsy called Lafora disease, and to curing this disease. We discovered the genes for this disease and made major inroads towards understanding its pathogenesis. This has allowed us insight into ways to treat this disease. The student will work one or another of the subprojects towards the above goals. Experience in the lab especially with molecular, cell culture, biochemical and mouse work will be a huge plus.



Seema Mital [email protected]


416-813-7718 The Hospital for Sick Children,


Project Title

Heart and Transplant Biobank.

Project Description

The Heart Centre Biobank Registry enrolls individuals with CHD for genetics/genomics research aimed at understanding the genetic basis of heart disease and genetic determinants of outcomes. Over 6000 individuals are enrolled with ongoing collections across several Ontario centres. Students will participate in: assisting in data collection, data entry, research tool development; obtaining family history, reviewing questionnaires, reviewing medical records; analyzing genetic & environmental risk factors for heart disease based on population; writing a proposal, gathering and analyzing data for their project and submitting results for presentation.

mailto:[email protected]



Istvan Mucsi [email protected]


416-340-4084 Toronto General Hospital, PMB

11C-188, 585 University Avenue, Toronto, ON

Project Title

Ethnocultural barriers to living donor kidney transplantation (LDKT).

Project Description

We investigate ethnocultural barriers to LDKT in patients with chronic kidney disease (CKD). Compared to dialysis or deceased donor kidney transplantation (KT), LDKT is the optimal treatment for most patients with advanced CKD. Compared to Caucasians, patients with East or South Asian or African Canadian background are less likely to undergo LDKT. The reasons for this ethnic disparity are unknown. In the study we assess ethnocultural background; transplant knowledge and readiness to accept LDKT using validated questionnaires. Questionnaires will be completed on iPad tablets. Hypothesis: Compared to Caucasians, patients of Asian or African background are less likely to have a potential LD identified and are less ready for LDKT. Two research sites are involved: Kidney Transplant Programs at Toronto General Hospital and at St. Michael’s Hospital. All consecutive patients who are referred for assessment for KT will be invited to participate. Target sample size is 600 patients. Students` role: Literature review; enrolling patients; collecting data using electronic data capture system; data entry, data cleaning; analysis of data using STATA statistical software; preparing abstracts, posters for conferences; writing manuscripts. Experience with STATA is an advantage.








Project Title

Validation of the PROMIS-57 Profile in Patients with Chronic Kidney Disease (CKD).

Project Description

We validate the PROMIS-57 (comprehensive quality of life profile) among patients with CKD. As symptoms of kidney failure become prominent, renal replacement therapy (RRT) (kidney transplantation or dialysis) is needed to stay alive. CKD and RRT are associated with impaired health related quality of life (HRQoL). In clinical practice HRQoL is rarely assessed. This is disturbing since HRQoL is an important outcome for patients. The PROMIS-57 measures various dimensions of HRQoL. It provides standardized scores that can be compared between different patient populations. It has not been validated in patients with CKD. In a cross sectional cohort study the validity of PROMIS-57 will be assessed by examining correlations between PROMIS-57 scores with scores obtained with legacy instruments (e.g., PHQ9, GAD7, KDQoL). Questionnaires are completed on iPad tablets. Hypothesis: the PROMIS-57 is valid and reliable among patients with various stages of CKD. Three research sites in Toronto: Toronto General Hospital; St. Michael’s Hospital; Humber River Hospital. Patients in dialysis units, kidney clinics and transplant clinics will be recruited. Students` role: Literature review; enrolling patients; collecting data using electronic data capture system; data entry, cleaning and analysis; preparing abstracts, posters for conferences; writing manuscripts. Experience with STATA is an advantage.








Project Title

Psychosocial barriers to living donor kidney transplantation (LDKT).

Project Description

We investigate psychosocial barriers to LDKT in patients with chronic kidney disease (CKD). Compared to dialysis or deceased donor kidney transplantation (KT), LDKT is the optimal treatment for advanced CKD. However, only 30-40% of kidney transplant recipients receive LDKT in Canada. The reasons for the underutilization of LDKT are not known. Modifiable psychological problems such as depression and avoidant attachment style may contribute. Both are important determinants of poor interpersonal and patient-healthcare provider relationship and may prevent effective communication with potential living donors. We assess depression, anxiety, and adult attachment style; readiness to accept LDKT using validated questionnaires. Questionnaires are completed on iPad tablets. Hypothesis: Depression and avoidant attachment is associated with reduced odds of having a potential LD identified and less readiness for LDKT. Two research sites are involved: Kidney Transplant Programs at Toronto General Hospital and at St. Michael’s Hospital. All consecutive patients, referred for assessment for KT will be invited to participate. Target sample size is 600 patients. Students` role: Literature review; enrolling patients; collecting data using electronic data capture system; data entry, data cleaning; analysis of data using STATA; preparing abstracts, posters for conferences; writing manuscripts. Experience with STATA is an advantage.




Daniel Mueller [email protected]


416-535-8501 x 36851 CAMH, 250 College Street, Rm

132, Toronto, ON M5T 1R8

Project Title

Pharmacogenomic studies on outcome to antidepressant treatment in late life depression.

Project Description

Approximately 10% of the elderly population are diagnosed with major depression (MDD) and are frequently prescribed antidepressant medications. However, up to 50% of older adults with MDD do not respond completely to initial antidepressant pharmacotherapy. Older adults with late-life depression are significantly more vulnerable to side effects of antidepressant medication, including antidepressant-induced osteoporotic and cardiovascular conditions. In order to investigate predisposition and genetic mechanisms involved in side effects, an interventional, randomized, prospective and double blind study, was conducted at different sites and at our institution (CAMH) (Lenze et al., The Lancet 2015). Patients aged 60 and older with MDD were enrolled. Patients were treated with venlafaxine (VF) for 12 weeks. Across multiple visits, patients were assessed using the Udvalg for Kliniske Undersøgelser (UKU) Side Effect Rating Scale. We have DNAs of each subject in our lab (N =468) and are currently conducting genome-wide genotyping. Our planned investigation will entail conducting a genome-wide association study (GWAS) to will yield novel gene variants which may be associated with side effects. Awareness of predisposing genetic factors for side effects in elderly MDD patients will be important in creating predictive tools to drive treatment selection and treatment personalization.



Andras Nagy [email protected]. Please

email Admin Assistant: [email protected]


416-586-3246 416-586-4800 x 8455

25 Orde Street, Rm 5-1015-3, Toronto, ON M5T 3H7 Mailing:

60 Murray Street, Box 40, Toronto, ON M5T 3L9

Project Title

The development of local-acting biologics to treat arthritis.

Project Description

The research undertaken by the student will focus on an ongoing project in my lab aimed at treating rheumatoid arthritis (RA). This devastating disease is primarily mediated by tumour necrosis factor alpha (TNF-α) and recently, our lab has developed local-acting biologics that target it in a joint-specific manner. The local-acting nature of our novel biologics are an attractive therapeutic strategy for RA as the systemic knock-down of TNF-α leads to systemic immune suppression. Currently, we are focused on evaluating the optimal cell type to produce these biologics within the joint. The summer student will assist a 3rd year PhD student in evaluating a variety of cell types that could survive inside the joints of our chosen mouse arthritis model. The project will involve the propagation and engineering of murine mesenchymal stromal cells from mouse embryonic stem cells, a potentially therapeutic cell type in the treatment of arthritis. The student will be taught a variety of techniques including Western blotting, immunostaining, cell culture, and confocal microscopy. Previous knowledge of molecular biology techniques would be beneficial. Furthermore, the student will need to attend training sessions to access the animal facility, the Toronto Centre for Phenogenomics.



Nades Palaniyar [email protected]


416-813-7654 x 302328 416-813-7654 x 303388

Peter Gilgan Centre for Research and Learning,

Hospital for Sick Children, 686 Bay Street, Toronto, ON M5G

0A4

Project Title

Neutrophil extracellular traps (NETs), infection, innate immunity, cell death, inflammation, lung, molecular mechanisms, therapeutics.

Project Description

Neutrophil extracellular traps (NETs) are unique innate immune structures cast to ensnare microbial pathogens present in the extracellular cavities. These NETs are made of neutrophil DNA and cytotoxic granular proteins. Therefore, although NETs are beneficial for the host, excess NETs can severely damage the host tissue and organs. Innate immune collectins such as surfactant protein D (SP-D) helps the functional properties of NETs. Whether SP-D regulates NETosis and NETs clearance is not fully understood. The SURP student will participate in a project that determines the steps involved in NETosis and NETs clearance. The project will typically involve isolating neutrophils from blood, inducing NET formation (LPS, bacteria, PMA, plate reader assays, confocal microscopy, Western blots, flow cytometry) and testing NETs clearance by alveolar macrophages (DNAse assays and endocytosis assays). This project is suitable for 3rd or 4th year students with a strong background in immunology and microbiology. Exposure to prior research work relevant to immunology would be preferable.



Elizabeth Pang [email protected]


416-813-6548 416-813-6660 (admin) Neurology, Hospital for Sick

Children, 555 University Avenue, Toronto, ON M5G 1X8

Project Title

Studying the brain language networks in children who suffered a neonatal stroke.

Project Description

Children who suffer a stroke in infancy will demonstrate language problems as they enter school and advance in their education despite preservation of their core spoken language skills. This preservation is puzzling as brain imaging often shows damage to classic language production areas. With advances in computational neuroscience, we have been able to study brain networks and brain connectivity. We would like to apply computational neuroscience methods to investigate how the brain reorganizes after a neonatal stroke to preserve core language functions and how these network changes in core language function mediate higher-level language difficulties. This project will be co-supervised by Dr. Elizabeth Pang, an MEG scientist, and Dr. Nomazulu Dlamini, a pediatric stroke specialist, at the Hospital for Sick Children. The focus of this project will be to use MEG imaging to examine the brain connectivity of language neural networks in school-aged and teenaged children who were affected by a stroke in infancy. Responsibilities will include recruiting patients, partnering with MEG staff to acquire the scan, using our analysis pipeline to compute MEG brain connectivity, and regular meetings for discussion with the co-supervisors and team. Background required: courses in cognitive neuroscience and some computer science or programming knowledge.



Ranju Ralhan [email protected]


416-586-4800 x 6426 416-586-4800 x 8211

Shnaider Laboratory of Research in Molecular Oncology, Mount Sinai Hospital, 600 University

Avenue, Rm 6-318, Toronto, ON M5G 1X5

Project Title

Identification of molecular drivers of malignant transformation in oral dysplasia.

Project Description

Oral squamous cell carcinomas (OSCCs) develop from lesions with epithelial dysplasia called oral premalignant lesions (OPLs). A major clinical challenge is lack of correlation between grade of dysplasia and risk of oral cancer development. There is an urgent need to identify molecular signatures that drive OPL transformation to cancer. These signatures are embedded in premalignant cells which are destined to progress to malignancy and could provide better predictive measures for assessing the risk of cancer development in OPLs. Objective. To unravel miRNAs and proteins as molecular drivers that lead to malignant transformation of oral dysplasia. Hypothesis. Identification of molecular drivers is based on our miRNA array with cells from un-transformed and transformed oral dysplasia and proteomic analysis; their validation in paired OPL and OSCC patient samples will provide insight into understanding molecular events relevant for malignant transformation. Hypothesis. Identification of molecular drivers is based on our miRNA array with cells from un-transformed and transformed oral dysplasia and proteomic analysis; their validation in paired OPL and OSCC patient samples will provide insight into understanding molecular events relevant for malignant transformation. AIM I. Analysis of candidate miRNAs and their target proteins expression in paired OPL and OSCC patients’ samples to identify molecular signature of malignant transformation. Aim II. Investigate effects of miRNAs/proteins as drivers of malignant transformation using oral dysplastic cell lines. This study will lead to better understanding of oral cancer development and biomarkers for predicting OPL patients at high risk of cancer.



Ori Rotstein [email protected]


416-864-5304 St. Michael's Hospital, 30 Bond Street, Toronto, ON M5B 1W8

Project Title

Remote Ischemic Conditioning in hemorrhagic shock.

Project Description

The student will investigate the cellular and molecular mechanisms whereby plasma derived from humans exposed to remote ischemic conditioning is able to inhibit neutrophil migration in the zebrafish. No background experience required aside from basic cellular biology techniques is required.



Susan Tarlo [email protected]


416-603-5177 Toronto Western Hospital,

EW7-449, 399 Bathurst Street, Toronto, ON M5A 4S9

Project Title

Respiratory effects of 3-D printing.

Project Description

3-D printing is being increasingly used both in industry and for personal projects. There is little known as to possible adverse respiratory effects from this. A pilot study is planned to investigate the exposures from 3-D printers and possible respiratory effects from these exposures. The student will participate in collecting samples from workplaces using 3-D printers, and in administration of questionnaires to users of 3-D printers, then assist in analysis of results. The student will present results at the IMS student research day.



Murray Urowitz [email protected]


416-603-5828 1E410B, Toronto Western


Project Title

Prognosis and biomarkers studies in systemic lupus erythematosus (SLE).

Project Description

The student will participate in one of a number of ongoing and proposed studies related to the prognosis of systemic lupus erythematosus. These studies include assessment of outcomes in these patients and the factors related to these outcomes. The student will collect clinical data, administer instruments of health status, collate information and participate in the analysis of the data. The student will be exposed to clinical research methodology as well as learn the clinical features, assessment and management of patients with rheumatic diseases.



Derek van der Kooy [email protected]


416-978-1960 416-978-0553 160 College Street, 11th Floor, Room 1102, Toronto, ON M5S

3E1

Project Title

Learning and memory in C. elegans.

Project Description

Our goal is to use the power and specificity of modern molecular genetics to reveal the component processes of learning and memory. In undertaking a mutational screening approach to learning and memory, we have taken advantage of the best-known multicellular organism, the nematode C. elegans. C. elegans has proven to be an excellent molecular model for mammalian (including human) biochemical functions. We will use the C. elegans learning and memory genes discovered to find their relevant mammalian homologues. Most important, the C. elegans mutants should allow us to ask if we can separate associative from non-associative learning, short from long-term memory, and learning and memory in one sensory modality from that in another sensory modality. DESCRIPTION OF STUDENT PARTICIPATION: The students will participate in the initial screens for new learning mutant worms, as well as all of the behavioural testing to determine if the deficits are in learning, memory storage of the recall of memories.



Rosanna Weksberg [email protected]


416-813-6386 416-813-8164 The Hospital for Sick Children,


Project Title

Aberrant DNA methylation in neurodevelopmental syndromes.

Project Description

Neurodevelopmental disorders encompass a diverse group of genetically heterogeneous disorders. Currently, we know that there are hundreds of genes involved, most of which have not yet been identified. The development of next generation sequencing has dramatically increased the speed at which new gene mutations causing neurodevelopmental syndromes can be identified. Many of these syndromes are caused by mutations in genes that encode modifiers of chromatin structure (epigenes). Our research focuses on neurodevelopmental disorders in epigene mutations which cause genome-wide DNA methylation alterations. Microarrays will be used to determine genome-wide DNA methylation patterns. The responsibilities of the student will be to validate candidate genes using pyrosequencing which includes assay design, determining the methylation status of the samples using the assay and analyzing the data. Our understanding of the role of genetic dysregulation in neurodevelopmental disorders will pave the way for better molecular tools for earlier diagnosis, more definitive prognosis and improved treatment.



Xiao-Yan Wen [email protected]


416-847-1737 416-864-6060 x 77623 Li Ka Shing Knowledge Institute,

209 Victoria Street, Toronto, ON M5B 1T8

Project Title

Molecular and Functional characterization of zebrafish gene trapped lines.

Project Description

Increasing evidence demonstrates that the zebrafish (Danio rerio) is a powerful model organism to study vertebrate development and organ function. The zebrafish has many remarkable features that make it more attractive than other vertebrate model organisms, including the external and rapid development of the embryos, embryonic transparency, short generation time, and ease of genetic manipulation. In this project, we are using gene trapping technology to randomly introduce insertional mutations across the zebrafish genome. A Tol2 transposon-based gene-trapping vector, called RP2 (Clark K., Nature Methods, 8:506-12, 2011), was used to mutate and fluorescently tag proteins in the zebrafish. The goal of the summer project is to identify and characterize trapped genes in selected zebrafish RP2 lines. Some of the experimental approaches that will be carried out, include: 1) Thermal Asymmetric Interlaced (TAIL) and Inverse PCR to identify the trapped genes. 2) In situ hybridization to demonstrate the trapped gene expression pattern. 3) Incrossing heterozygous gene-trapped siblings to analyze the phenotype(s) of homozygous mutants. 4) Rescuing the mutant phenotype using tissue specific cre-recombinase zebrafish lines to validate the gene function.



Cari Whyne [email protected]


416-480-5056 416-480-5858 2075 Bayview Avenue, S620,

Toronto, ON

Project Title

Image Analysis in the Spine.

Project Description

The Orthopaedic Biomechanics Laboratory at Sunnybrook has experience developing novel image analysis software to assist clinicians with decision making and treatment planning for the spine. This project will advance and apply semi-automated methods to quantify imaging of the spine allowing for fast and reliable calculation of CT-based metrics that were demonstrated to be important for fracture stability and treatment planning. The student will be involved in ongoing development to incorporate our algorithms into clinically focused software requiring intuitive and powerful user interfaces. Improvements to the treatment planning/simulation may focus on the development and implementation of algorithms and workflows for automated detection of an optimized trajectory for pedicle screw surgery in the spine and/or improvements in fracture risk assessment in metastatically involved vertebrae. This work will combine CT image analysis tools for quantifying vertebral anatomy, spinal cord, tumour, etc. with clinical scoring methods to aide clinical decision making. This treatment planning / risk assessment software will ultimately aide in managing complex patients. Skills beneficial to complete this work include a background in engineering, medical biophysics or computer science with experience in image analysis, programming languages (C and C#), user-interface design, as well as some familiarity with medical imaging and basic anatomy.



Albert Wong [email protected]


416-535-8501 x 34010 416-535-8501 x 6233

Centre for Addiction and Mental Health, 250 College

Street, Room 323, Toronto, ON M5T 1R8

Project Title

Clinical and animal model studies in neuropsychiatric disorders.

Project Description

There are a variety of projects that students can be involved with, all aimed at understanding more about the neurobiology of mental illness. 1) Mouse models. Students can be involved with experiments to characterize genetic mutant mouse models for schizophrenia and the testing of novel drugs to determine potential as treatments for psychiatric disease. Methods include behavioral testing, histology and biochemistry. 2) Virtual reality-based cognitive testing. Custom VR city environments are used to probe spatial navigation and other cognitive functions in a naturalistic setting, with the goal of investigating cognitive dysfunction in schizophrenia and for use as a treatment tool. 3) Clinical biomarker studies in PTSD and schizophrenia. Students can assist with recruiting and assessing patients with psychiatric disease, and with biochemical analysis of biological samples.



Haibo Zhang [email protected]


416-864-6060 x 77654 416-864-6060 x 77648 Li Ka Shing Knowledge Institute,

209 Victoria Street, Rm 619, Toronto, ON M5B 1T8

Project Title

Mechanisms of human neutrophil peptides (HNP) mediated inflammatory responses.

Project Description

Introduction: HNP are small cationic antimicrobial peptides stored in azurophilic granules of neutrophils and are released during lysis. Rationale: We have shown that HNP can induce expression of the adhesion molecule ICAM-1 and co-stimulatory molecule CD80 and CD86 in human lung epithelial and endothelial cells and CD28 and CD152 in human CD4+ T cells. Since chemotactic movement is essential for leukocyte interaction with endothelial cells resulting in subsequent extravasation, we hereby will examine whether HNP play a role in cell-cell interaction during inflammation. Hypothesis: HNP modulate host immunity through an enhanced interaction between endothelial and leukocyte cells by up-regulation of surface adhesion molecules. Experimental Plan: Anesthesized C57BL/6J mice will receive an i.v. injection of purified HNP or vehicle buffer. Leukocyte rolling velocity, leukocyte-endothelial interaction will be examined by intravital microscopy in post-capillary venules for 5 hours. Expression of the adhesion molecules mentioned above and inflammatory cytokines (TNF- -2 and GM-CSF) will be determined in the tested tissue at gene and protein levels. Based on our in vitro data, we expect an increased leukocyte adhesion to the endothelium resulting in excessive cytokine release.



Liang Zhang [email protected]


416-603-5800 x 2702 416-603-5800 x 2209

7KD-407, 60 Leonard St., Toronto Western

Hospital/Toronto Western Research Institute, Toronto, ON

M5T 2S8

Project Title

Mechanisms and potential treatments of post-ischemic epileptic seizures in mouse models.

Project Description

Brain ischemia is a major cause of epilepsy in children and aging individuals, but underlying pathological process is not well understood. We aim to establish reliable mouse models to explore the mechanisms of epileptogenesis following brain injury and to test potential treatment strategies. Briefly, we will conduct brain ischemic episodes in immature or aged mice and examine post-ischemic seizures via continuous video monitoring and/or electroencephalographic (EEG) recordings. We will determine the time frame in which recurrent seizures develop and progress post ischemia and explore whether early anti-antiepileptic treatments are helpful in preventing or decreasing late-onset epileptic seizures. The student is expected to read relevant literature and actively participate in ongoing experiments. Specifically, his or her tasks are: 1) to participate in ischemia-inducing manipulations, 2) to carry out video/EEG monitoring and drug injection in post-ischemic animals, 3) to analyze video/EEG data and perform statistical tests, and 4) to conduct brain histological experiments (tissue section, staining and image process). The student is expected to present his or her data in institution research day or other local scientific events.

Documents

IMS Supervisors Recruiting 2016 SURP students · 2017-07-14 · IMS Supervisors Recruiting 2016 SURP students First Name Family Name Email Anne Bassett [email protected] Office