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S1. C/EBP a and E6AP co-localize in the nucleus. HEK alone. HEK+C/EBP α. C/EBP α + E6AP 1.0 μ g. C/EBP α + E6AP 2.0 μ g. Merge. DAPI. E6AP. C/EBP α. S2. GST-E6AP Purification. GST-E6AP+IPTG. GST alone. GST-E6AP. Marker. 1 2 µg(BSA). GST-E6AP. BSA. GST. S3a. - PowerPoint PPT Presentation
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HE
K a
lon
eH
EK
+C
/EB
Pα
C/EBPα E6AP DAPI Merge
C/E
BP
α+
E6A
P 2
.0μ
gC
/EB
Pα
+ E
6AP
1.0
μg
S1. C/EBP and E6AP co-localize in the nucleus
S2. GST-E6AP Purification
GST
-E6A
P+IP
TG
GST
alo
ne
1 2 µg(BSA)
GST-E6AP
GST
BSA
Mar
ker
GST
-E6A
P
β actin
p42C/EBPα-ER 67 kDaK
562
1 2 3 4 5 6 clone K562 p42C/EBPα-ER
K562-p42C/EBPα -ER clone2 5µM E2 , 5days
K562-p42C/EBPα-ER clone2
K56
2K
562+
p42C
/EB
Pα-E
R c
lone
2
HEK
alo
neH
EK +
p42C
/EB
Pα
42kDa
67 kDa
S3a.
b. c.
Fig. S4: E6AP is expressed in various myeloid leukemia cell lines
E6AP 100kDa
β actin
HE
K29
3T a
lone
HE
K29
3T+C
/EB
Pα+
E6A
P
HL6
0U
937
K56
2
Kas
umi1
HEK
alon
eH
EK+E
6AP
MC
F7M
CF7
+siR
NA
E6A
PK
562+
siR
NA
E6A
P
K56
2
100kDa E6AP
β actin
Fig. S5: siE6AP efficiently down regulates E6AP expression
E6AP100kDa
C/EBPα 42kDa
C/E
BP
α
C/E
BP
α+
MG
132
C/E
BP
α+
MG
132
C/E
BP
α
K56
2 al
one
K56
2 al
one
IP Ig
G
IP E6AP
Fig. S6: Ectopically expressed C/EBPα physically interacts with E6AP in K562 cells
