Half-yearly Progress Report - National Food Security Missionnfsm.gov.in/Project/ICRISAT/2015-16/NFSM-Pigeonpea... · The detailed report of survey is provided in Annexure 2. RAKCA

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Half-yearly Progress Report April–September 2015

Addressing Phytophthora Blight Disease: An Emerging Threat to Pigeonpea Expansion and Production

Submitted to

National Food Security Mission (NFSM) Department of Agriculture & Cooperation, Ministry of Agriculture

Government of India

NFSM Kisan ki Unnati Desh ki Pragati

This work is being undertaken as part of the

In partnership with

2

Collaborating Institutions and Scientists

International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Telangana Mamta Sharma

Raju Ghosh

CV Sameer Kumar

RAK College of Agriculture (RAKCA), Sehore, Madhya Pradesh DR Saxena

AK Chaudhary

Banaras Hindu University (BHU), Varanasi, Uttar Pradesh Ramesh Chand

Vineetha Singh

Punjab Agricultural University (PAU), Ludhiana, Punjab Asmita Sirari

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Project Title: Addressing Phytophthora Blight Disease: An Emerging Threat to Pigeonpea Expansion and Production

(F.No.CPS-5-11/2013-NFSM)

Funding Agency: National Food Security Mission (NFSM) - Pulses, Department of

Agriculture & Cooperation, Ministry of Agriculture, Govt. of India

Executive Agency: International Crops Research Institute for the Semi-Arid Tropics

(ICRISAT), Patancheru – 502 324, Telangana

Principal Investigator:

Dr Mamta Sharma, Senior Scientist, Legumes Pathology, International Crops Research

Institute for the Semi-Arid Tropics (ICRISAT), Patancheru – 502324, Telangana

Co- Investigators:

BHU, Varanasi: Dr Ramesh Chand (PI), Dr Vineetha Singh (Co-PI),

RAKCA, Sehore: Dr DR Saxena (PI), Dr AK Chaudhary (Co-PI)

PAU, Ludhiana: Dr Asmita Sirari (PI)

Project Duration: 4 years

Objectives

1. Identify pigeonpea genotypes with resistance to Phytophthora blight of pigeonpea.

2. Evaluate resistant genotypes for stability of resistance at multi-locations.

3. Evaluate Phytophthora blight resistant genotypes in farmers’ fields.

4. Technology exchange and capacity building in integrated management of

Phytophthora blight.

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Technical progress (April-September 2015)

1. Survey: Monitoring for occurrence and distribution of Phytophthora blight

Survey was conducted by all the project parteners (ICRISAT, BHU, PAU, and RAKCA) in their

respective and nearby states [southern India (Telangana, Andhra Pradesh, Karnataka, and Tamil

Nadu), northern India (Uttar Pradesh and Punjab) and central India (Madhya Pradesh)]. The disease

is distributed throughout the pigeonpea cultivation area. Phytophthora blight (PB) was found on

average from 60% of fields, but its severity was low this year. Fields with high number of dead plants

and plants with severe symptoms was rare due to deficit in rainfall as compared to previous years.

According to the survey, and greenhouse and field experiments, heavy reinfall and high humidity

during the growth period favor disease development and can cause significant yield losses. Details of

survey results are as follows:

Total 50 fields were surveyed in 3 states (Karnataka, Telangana and Madhya Pradesh) by ICRISAT.

Incidence ranged from 0-50% (Annexure 1) (Figure 1). The detailed report of survey is provided in

Annexure 1 and natural incidence of PB in pigeonpea fields is given in figure 1.

Figure 1. Natural PB incidence during survey 2015-16 crop season.

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Total 34 fields were surveyed in eastern Uttar Pradesh by BHU scientists. Incidence was recorded on

35-40 days old seedling in four places. Typical stem necrosis near the node was observed (Figure 2).

On a few plants leaf infection was also recorded. The detailed report of survey is provided in

Annexure 2.

RAKCA conducted survey of pigeonpea growing areas of Madhya Pradesh state was conducted in the months of September, 2015. Bund cultivation of pigeonpea was very common in the state. Farmers did not adopt seed treatment in pigeonpea. PB incidence in farmers field ranged from 3-53%. The detailed report in the prescribed proforma is provided in Annexure 3.

Survey for occurrence of PB was done at farmer’s field in different villages of district Hoshiarpur and Ludhiana by PAU. Incidence of PB ranged from 0.5-20% at farmer’s field (Figure 3). However, high incidence of PB was recorded on experimental farms of PAU, Ludhiana, showing 2-90% (Annexure 4). During survey, it was observed that lowest disease incidence was observed in case of intercropping of pigeonpea with groundnut (2%) whereas maximum disease incidence was recorded in case of intercropping of pigeonpea with maize (90%).

Figure 2. PB symptoms during 2015-16 crop season Uttar Pradesh.

Figure 3. Natural epidemic of PB in experimental fields at PAU, Ludhiana.

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2. Updated list of P. cajani isolates from different pigeonpea growing areas of

India

2.1. Isolate collection status

Similar to year 1 and 2, pigeonpea plants exhibiting symptoms of PB were collected from epidemic block in the field from different locations. Symptomatic plant materials were placed in labelled plastic bags, which were transported in cooler box and stored in 4°C until fungal culture were purified from the samples in the laboratory (Legumes Pathology, ICRISAT). Stem tissues with typical PB lesions were selected for isolation of the Phytophthora. Symptomatic tissues were cut and surface sterilised by 1% sodium hypochlorite for 1 min followed by washing in sterile distilled water. The tissues were then plated onto sterilized V8 juice agar media (V8 juice supplemented with L-Asparagine, CaCO3, glucose, yeast extract and agar) amended with PARP antibiotics (pimarcin 400 μL; ampicillin 250 mg; rifampicin 1,000 μL; and pentachloronitrobenzene 5 ml l-1 media). Plates were incubated at 25°C in the 12 h/12 h day-night photoperiod for 3 to 4 days. Putative Phytophthora colonies were transferred to 20% tomato extract agar (tomato extract 200 ml, CaCO3 2 g and agar 20 g l-1) and incubated at 25°C in the 12 h/12 h day-night for 7 days. Cultures were then maintained under in vitro at 18 ±1˚C in dark condition. To maintain the pathogen virulence, new cultures were prepared every 2-3 months by passing the pathogen through the host and reisolating from infected plants. Total 28 isolates were purified and identified as Phytophthora cajani and 14 isolates have been deposited in NCBI genebank (Table 1).

Table 1. Details of P. cajani isolates from different pigeonpea growing areas of India.

S. No. Isolates Sampled from Length of ITS

(nt)

Accession number

1 ICPC1 Varanasi, UP 504 KJ010534

2 ICPC2 BP09 534 KJ010535

3 ICPC3 RM1 728 KJ010536

4 ICPC4 BP5 698 KJ010537

5 ICPC5 BP15 518 KJ010538

6 ICPC6 Mahabubnagar, AP 772 KJ622200

7 ICPC7 Adilabad, AP 570 KJ622201

8 ICPC8 DHF4 698 KJ622202

9 ICPC 9 DHF4 467 KJ622203

10 ICPC10 BP14 686 KJ622204

11 ICPC11 BP14 506 KJ622205

12 ICPC12 BP14 687 KJ622206

13 ICPC13 BP14 749 KJ622207

14 ICPC14 BP14 822 KJ622208

15 ICPC 15 BP14 - -

16 ICPC 16 Ludhiana - -

17 ICPC 17 RCW 01 - -

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S. No. Isolates Sampled from Length of ITS

(nt)

Accession number

18 ICPC 18 Khochawa (Varanasi) - -

19 ICPC 19 Khochawa (Varanasi) - -

20 ICPC 20 Anantpur (Mirzapur) - -

21 ICPC 21 Jakhhine (Varanasi) - -

22 ICPC 22 Mahboobnagar (TS) - -

23 ICPC23 Mahboobnagar (TS) - -

24 ICPC 24 Raichur (KA) - -

25 ICPC 25 Raichur (KA) - -

26 ICPC 26 Gulberga (KA) - -

27 ICPC 27 Gulberga (KA) - -

28 ICPC 28 Sehore (MP) - -

3. Identification of resistance sources

Screening of germplasm and breeding lines as well as confirmation of resistant sources were continued at different partners locations to identify the resistance sources to PB disease. Location-wise details of the material evaluated and their reaction to PB is described in the following section.

ICRISAT : Controlled environment screening using zoospore suspension

inoculation technique

Pigeonpea composite core collection: Total 1,251 accessions from pigeonpea composite

core collection representing wide diversity in germplasm collection worldwide were

screened for PB under greenhouse using standardized zoospore screening technique (Figure

4). Initial screening showed that out of 1,251 accessions, 58 lines were resistant with <10%

incidence and 51 lines moderately resistant having incidence between 10-20%. Remaining

lines were susceptible/highly susceptible to PB. These lines will be evaluated again to

confirm the resisnce to PB (Table 2).

Pigeonpea AVT, IVT lines from AICRP : Total 84 elite lines from AICRP-pigeonpea were

evaluated against PB. Seven lines were found resistant with <10% incidence, 9 lines with

moderate resistance having 10- 20% incidence (Table 2).

Sehore–PB resistant lines: Total 12 breeding lines found resistant at RAKCA Sehore were

evaluated for PB at ICRISAT. Three lines showed moderate resistance against PB (Table 2).

PAU–PB resistant lines: Total five breeding lines found resistant to PB at PAU were

evaluated for PB at ICRISAT. One line showed resistance against PB (Table 2).

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Table 2. Pigeonpea germplasm and breeding lines evaluated to Phytophthora blight at ICRISAT under controlled environment using zoospore suspension.

S. No Detail

No. of Lines

Screened

PB incidence (%)

0-10 11-20

1 Pigeonpea FW,SM and FABL Lines

59 ICPL 99010 ICPL 99102, ICPL 8859

2 Pigeonpea composit Core collection lines

1251 ICP 3925, ICP 4015, ICP 4488, ICP 5275, ICP 5346, ICP 6788, ICP 7057, ICP 7323, ICP 8211, ICP 8516, ICP 8522, ICP 8675, ICP 8700, ICP 8757, ICP 8776, ICP 8921, ICP 8928, ICP 8996, ICP 9045, ICP 11213, ICP 11230, ICP 11592, ICP 11823, ICP 14653, ICP 15091, ICP 15095, ICP 15401, ICP 6035, ICP 7426, ICP 8266, ICP 8914, ICP 14975, ICP 3945, ICP 4089, ICP 5239, ICP 5240, ICP 5252, ICP 5335, ICP 5455, ICP 6419, ICP 8558, ICP 8568, ICP 8891, ICP 9764, ICP 11277, ICP 11785, ICP 4605, ICP 4822, ICP 8562, ICP 8793, ICP 9096, ICP 11361, ICP 12334, ICP 4167, ICP 5388, ICP 7242, ICP 8131, ICP 8265

ICP 2467, ICP 3955, ICP 4029, ICP 4317, ICP 4983, ICP 7072, ICP 7284, ICP 8227, ICP 8368, ICP 8685, ICP 8817, ICP 8997, ICP 9252, ICP 10404, ICP 16264, ICP 16266, ICP 4241, ICP 2641, ICP 4519, ICP 4903, ICP 5325, ICP 5515, ICP 7269, ICP 8784, ICP 11097, ICP 11769, ICP 2500, ICP 3451, ICP 3842, ICP 4266, ICP 7172, ICP 8132, ICP 8369, ICP 10241, ICP 14900, ICP 4257, ICP 2463, ICP 5255, ICP 7370, ICP 7701, ICP 11246, ICP 4476, ICP 5066, ICP 5161, ICP 4633, ICP 4991, ICP 5006, ICP 6929, ICP 8382, ICP 8537, ICP 10615

3 Evaluation of AICRP materialas AVT, IVT,

84 PA 426, WRG 246, WRG 286, GRG 2009-1, WRG 285, KPL 43, KA 12-2 (07)

CORG 9701, CO 6, RVSA 2014-1, WRG 280, WRG 293, KPL 44, WRG 242, WRG 297, WRG 252 (09)

4 Pigeonpea RAK Shehore lines

12 Nil RAKCA RVS 7-10, RAKCA SRGK-1, RACA RVS 7-22

5 Pigeonpea PAU lines

5 PAU AL 1836 Nil

6 Extra early lines (DT)

12 ICPL 11253 ICPL 11256, ICPL 20336, ICPL 11255, ICPL 11249, ICPL 20338

7 Extra early lines (NDT)

22 ICPL 11245, ICPL 20333, ICPL 20329

ICPL 20326, ICPL 11318, ICPL 20327, ICPL 20335, ICPL 11244, ICPL 20325, ICPL 20328, ICPL 11298, ICPL 11324

8 WHDLE Lines

10 Nil ICPL 15009, ICPL 15006

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S. No Detail

No. of Lines

Screened

PB incidence (%)

0-10 11-20

9 Lines from Kalyani University, West Bangal

5 Richa (MP) Desi Tur (MP)

10 Lines from UAS, Raichur

50 AKT 8811, RKV 277 ICP 11320, Kari togari, Raichur pink, RVK 275

Total 1510 74 86

Figure 4. Evaluation of pigeonpea genotypes for PB at ICRISAT using zoospore suspension.

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Confirmation of PB resistance Total 2,572 lines including germplasm and breeding lines have been screened so far at ICRISAT using the zoospore suspension technique under greenhouse. From this prelimnary screening, 77 lines with resistance to PB at ICRISAT were selected for further confirmation (Figure 5). Approximately 100 seeds of each line was inoculated and incubated under favourable environmental conditions. Three lines with high level of resistance and nine with moderate level of reisistance were selected for futher testsing at multi-locations. From BHU selections, 91 lines werwe further evaluated at ICRISAT and 15 were found resistant and 9 moderately resistant (Table 3).

Table 3. Confirmation of resistnace to Phytophthora blight at ICRISAT under controlled environment using zoospore suspension.

Sno Particular of Source

No. of Lines Screened

Phytophthora blight incidence %

0-10 11-20

1 Revaluation of PB resistance lines

77 ICPL 20331, ICPL 11273, ICPL 11254

ICPL 99048, ICPL 11254, ICPL 99009, ICPL 20236, ICPL 11265, MN-1, ICPL 11270, ICPL 20114, ICPL 20331

3 Revaluation of pigeonpea BHU lines

91 BHU 020, BHU 082, BHU 269, BHU 279, BHU 296, BHU 302, BHU 303, BHU 307, BHU 347, BHU 450, BHU 451, BHU 464, BHU266, BHU 274, BHU 343

BHU 218, BHU 275, BHU 217, BHU 267, BHU 465, BHU 054, BHU 559, BHU 339, BHU 503

Total 168 18 18

11

Susceptible Resistant

Susceptible Resistant

Figure 5. Confirmation of PB resistance in pigeonpea genotypes at ICRISAT.

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BHU - Screening for PB resistance

Nodal inoculation technique was further refined at BHU for screening germplasm and breeding lines. The disease scale (1 to 10) proposed to rate the lesions produced on the stem is given in Figure 6, where 1= Lesions restricted to inoculation point of node with normal growth of plant; 2= Minute lesion around infection point and plant growth normal; 3= Lesion areas irregular approx. 0.5cm in size at inoculation point and plant growth normal; 4= Lesion areas approx. 1 cm, appear dark brown in colour and plant resumed normal growth; 5= Lesion size increases with brown to black colour on infected stem and plants survived; 6= Lesion extended several centimetre, dark brown in colour and plants survived; 7= Lesions become dark brown to black in colour and girdling started in stem, some plants survived; 8= infected stem becomes dry, weakened and likely to topple down; 9= Stem dried, slightly blackish in colour and upper leaves dried; 10= Infected stem completely dried and plant died.

Figure 6: Disease rating scale (1-10) for PB resistance screening. (The lesions scored 1-3 were rated resistant (R); 4 to 5 as moderately resistant (MR); 6 - 7 as susceptible (S) and 8 - 10 as highly susceptible (HS)).

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Screening of 600 lines collected from farmer’s fields for PB resistance:

The 600 lines were collected last year from eastern Uttar Pradesh. These lines were sown under natural field conditions. Plants were inoculated with the mycelial disc of 5 mm diameter from 7 days old culture and inoculated on 30 days old seedlings. Ninety six hours after inoculation, data was recorded on 10 plants in each line (Figure 7).

Figure 7: Disease reaction of 600 lines collected from farmer’s fields at BHU for PB.

RAKCA-Sehore – Screening for PB disease resistance

Screening of pigeonpea AICRP lines: Total 40 AICRP pigeonpea lines were screened in PB sick plot during 2015-16. The entries were sown very late on 24 August 2015 in two replications alternated with susceptible check ICP 7119. Data recorded till date showed 12 lines (SJP 102, BRG 3, WRGE 140, KDVP 1935, RPS 2007-10, KPL 44, BRG 5,CRG 2012-20, AL 2046, CRG 2013, WRGE-92, CO6 [ch]) resistant to PB (Annexure 5) However, final data will have to be collected.

PAU-Ludhiana – Screening for PB resistance

Screening of Pigeonpea released/advanced germplasm & breeding lines: A set of 100 advanced breeding lines from Ludhiana centre and 112 Pigeonpea germplasm lines from different centers are being evaluated against PB. Both mini sick plot and greenhouse technique are being used for reliable screening of pigeonpea breeding and germplasm lines.

Mini sick plot technique: A set of 164 test entries were sown in 4m row with 40 cm row spacing on 11 June 2014. The susceptible check AL201 was sown after every fourth entry. The inoculum of PB was mass multiplied on sterilized pigeonpea grains. In first week of July, the inoculum was mixed with soil and smeared on stems of test entries. The inoculum was also added in the soil at the time of sowing to develop a sick plot. The water logging

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conditions were maintained till 45 days. The stem showed black discoloration with blighting of foliage. First observation on blighted plants has been recorded and given in table 4. Final observations on disease incidence will be recorded as diseased plants percentage.

Green house technique: Sowing of 10 seeds per test entry was done on pots with soil supplemented with PSB inoculum. When the seedlings were 20 days old, the inoculum was smeared on stem of seedlings and pots were watered regularly for 7 days. The observations on blighted plants have been recorded. The percentage disease incidence will be calculated and final disease response of test entries to PB will be recorded.

Confirmation of PSB resistance: A set of seven pigeonpea lines from ICRISAT, 12 from RAKCA, Sehore and 441 entries from BHU were evaluated for confirmation of PB resistance.

Table 4: Disease reaction of Pigeonpea germplasm lines to PB

Sr. no. Entry PSB incidence

(%) Sr. no. Entry

PSB incidence

(%)

1 AL-1843 16.58 43 AL-2102 18.55

2 AL-1931 17.64 44 ICPL-11301 17.04

3 AL-1932 23.91 45 AL-1992 24.24

4 AL-1933 50.08 46 AL-2024 35.83

5 AL-1957 41.04 47 AL-2128 29.69

6 AL-1960 37.50 48 ICPL-20336 26.11

7 AL-1965 52.10 49 ICPL-201153 18.63

8 AL-2000 42.66 50 MN-1 (CH) 16.35

9 AL-2009 43.59 51 MN-5 (CH) 19.85

0 AL-2046 30.83 52 AL-15 (CH) 28.04

11 AL-2049 35.14 53 AL-1760 10.00

12 ICPL-11301 25.63 54 AL-1765 18.06

13 AL-1758 27.53 55 AL-1779 36.67

14 AL-1849 44.08 56 AL-1781 0.00

15 AL-1922 21.47 57 AL-1782 31.25

16 AL-2019 40.00 58 AL-1786 0.00

17 AL-2021 31.99 59 AL-1790 0.00

18 AL-2025 32.38 60 AL-1793 17.07

19 AL-2028 25.96 61 AL-1789 8.33

20 AL-1994 27.08 62 AL-1798 5.00

21 AL-1976 41.03 63 AL-1801 5.00

22 AL-2060 25.14 64 AL-1813 0.00

23 AL-2061 17.76 65 AL-1820 18.22

24 AL-2062 24.04 66 AL-1840 6.67

25 AL-2063 12.26 67 AL-1845 25.00

26 AL-2067 11.66 68 AL-1846 37.50

27 AL-2071 16.40 69 AL-1848 7.28

28 AL-2078 18.42 70 AL-1853 17.50

29 AL-2086 25.55 71 AL-1837 7.14

30 AL-2087 24.06 72 AL-1842 26.88

31 AL-2122 41.60 73 AL-1844 Not germinated

32 AL-2016 15.09 74 AL-1847 4.20

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(%) Sr. no. Entry

PSB incidence

(%)

33 AL-2028 15.02 75 AL-1957 0.00

34 AL-2088 11.04 76 IC245-318 Not germinated

35 AL-2090 17.70 77 IC245-350 23.81

36 AL-2091 6.61 78 H02-59 21.27

37 AL-2092 13.88 79 H94-8 10.52

38 AL-2094 17.13 80 H200014 19.23

39 AL-2095 13.60 81 IC245-183 12.75

40 AL-2096 13.13 82 IC245-186 18.51

41 AL-2098 9.65 83 IC245-219 12.22

42 AL-2101 21.33 84 IC245-314 12.60

85 IC245-409 0.00 125 Pant A-250 17.16

86 IC245-439 11.81 126 Pant A-251 14.26

87 IC245-443 22.22 127 Pant A-252 11.54

88 IC245-479 12.50 128 TAT-108 33.33

89 IC45561 12.50 129 SP-2-2 0.00

90 IC45362 15.76 130 Pusa-855 25.00

91 H03-28 4.00 131 AL1505 6.98

92 H86-22 6.25 132 AL1506 14.47

93 H92-14 4.55 133 AL1508 9.38

94 H93-32 14.29 134 AL1524 21.43

95 AF-370 4.17 135 AL1525 16.21

96 CORG-108 0.00 136 AL1527 7.76

97 CORG-105 0.00 137 AL1584 4.50

98 Pusa2001 4.03 138 AL1627 13.12

99 P-2002 15.88 139 AL1628 15.00

100 H02-65 0.00 140 AL1629 5.92

101 H05-7 7.14 141 AL1634 11.81

102 H05-35 10.00 142 AL1847 14.58

103 AH06-1 4.17 143 CORG 9704 6.25

104 AH06-3 5.56 144 ICPL 90245 0.00

105 AH06-9 0.00 145 Pusa 2001 13.57

106 AH09-3 22.50 146 P-226 0.00

107 AH09-36 8.82 147 HOL 59 17.38

108 13-71AP 0.00 148 H005 11.69

109 VRG-62 0.00 149 HO2-45 7.14

110 V10099 0.00 150 HO4-20 3.57

111 ICPL92045 16.67 151 HO5-7 15.00

112 MTH-103 16.67 152 HO5-12 60.00

113 ICP11250 16.67 153 HO5-33 8.33

114 ICP3977 Not germinated 154 HO5-62 0.00

115 ICP45523 0.00 155 H200014 36.46

116 IC245507 0.00 156 AH-09-11 9.00

117 IC245/Pusa991 50.00 157 AH-09-29 9.09

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(%) Sr. no. Entry

PSB incidence

(%)

118 IC245-504 5.56 158 AH-09-30 15.38

119 PBR-2 0.00 159 AH-09-41 0.00

120 P226 7.14 160 H86-22 14.24

121 P2001-6 2.94 161 H-93-2 8.67

122 Pant-37 0.00 162 H-94-8 42.11

123 Pant A-169 12.50 163 H-93-32 10.00

124 Pant A -234 3.13 164 P-95-1-1 9.09

1. Selection of fungicides and sensitivity testing against Phytophthora cajani

Based on the literature, list of fungicides were selected for testing their efficacy against P. cajani in vitro as well as under greenhouse conditions. List of fungicides selected is given in Table 5. These fungicides has also been provided to the project partner’s for testing against the respective isolate of their locations. The fungicide testing against P. cajani is in progress at all the centers.

Table 5. Fungicide selected for efficacy study against P. cajani

S No Fungicide (Trade name) Active ingredient

1. Ridomil gold Metalaxyl (4%) + Mancozeb (64%)

2. Indofil M-45 Mancozeb (75%) WP

3. Curzate M-8 Cymoxanil (8%) + Mancozeb (64%)

4. Nativo Tebuconazole (50%) + Trifloxystrobin (25%)

5. Acrobat Dimethomorph (50%) WP

6. Equation pro Famoxadone (16.6%) + Cymoxanil (22.1%)

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2. Publications

3. Acknowledgements

We gratefully acknowledge the National Food Secrtiy Mission, Govt. of India, Ministry of Agriculture, Department of Agriculture and Cooperation, New Delhi for providing funding support for this research project. We extend our sincere thanks to the SAU’s and regional research centres for helping in conducting the surveys.

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Annexures

Annexure 1. Occurrence and distribution of Phytophthora blight in major pigeonpea growing areas in India during 2015-2016 crop season

S No District Village PB incidence (%)

Madhya Pradesh

1 Sehore RAKCA 16.1

2 Sehore RAKCA 0.0

3 Sehore RAKCA 6.9

4 Sehore Magaspur 45.7

5 Sehore Gattakheda 24.3

6 Sehore Bhaukhedi 4.4

7 Sehore Pangra 32.9

8 Sehore Pangra 27.1

9 Sahajapur Ranayal 46.3

10 Sahajapur Pochaner 0.0

11 Sahajapur Pochaner 4.2

12 Sahajapur Amlaha 6.1

13 Sahajapur Amlaha 0.0

14 Rajgadh Bavdikheda 0.0

15 Rajgadh Bavdikheda 0.0

16 Rajgadh Malkhedi 0.0

17 Rajgadh Eklera 4.6

18 Hoshangabad Suhagpur 0.0



21 Raisen Mangrul 5.5

22 Raisen Mangrul 2.0

Telangana

23 Mahboobnagar Marikal 22.5

24 Mahboobnagar Eliganela 7.6



27 Mahboobnagar Magnoor 11.4

28 Mahboobnagar Indanoor 2.6

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29 Ranga Reddy Tandoor 0.0



Karnataka

28 Raichur UAS 0.0

32 Raichur UAS 8.1

33 Raichur Masarkal 48.5






39 Gulbarga ARS 22.7

40 Gulbarga Kalnoor 49.5

41 Gulbarga Gundargurti 43.3



44 Gulbarga Malkhed 5.8

45 Gulbarga Malkhed 42.5

46 Gulbarga Unkanade 9.6

47 Gulbarga Bomanhalli 38.6

48 Gulbarga Vibbali 13.1

49 Gulbarga Adaki 16.0

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Annexure 2- Pigeonpea disease survey conducted in Varanasi during 2015-16 (BHU- Varanasi) S

No.

Village Name Date of

collection

GPS Value Fungi identified

1. Adalpura (Mirzapur) 02/09/2015 ELEV: 382 Ft

N 250 11.052’

E 0820 53.166’

-

2. Madeya (Mirzapur) 02/09/2015 ELEV: 541 Ft

N 250 08.434’

E 0820 52.201’

-

3. Madeya (Mirzapur) 02/09/2015 ELEV: 521 Ft

N 250 08.430’

E 0820 52.203’

-

4. Madeya- Adalpura

(Mirzapur)

02/09/2015 ELEV: 510 Ft

N 250 08.427’

E 0820 52.201’

Phytophthora

5. Katerhawa- Mediya

(Mirzapur)

02/09/2015 ELEV: 198 Ft

N 250 07.929’

E 0820 51.597’

-

6. Katerhawa (Mirzapur) 02/09/2015 ELEV: 205 Ft

N 250 07.615’

E 0820 50.985’

Phytophthora

7. Bagaha (Mirzapur) 02/09/2015 ELEV: 210 Ft

N 250 07.615’

E 0820 50.985’

-


N 250 10.055’

E 0820 48.193’

-


N 250 10.182’

E 0820 48.146’

-


N 250 10.181’

E 0820 48.147’

-

11. Jalalpur (Mirzapur) 02/09/2015 ELEV: 224 Ft

N 250 15.048’

E 0820 49.125’

-

12. Jogapur (Varanasi) 02/09/2015 ELEV: 258 Ft

N 250 12.578’

E 0820 50.704’

-

13. Maniyaripur (Varanasi) 02/09/2015 ELEV: 218 Ft

N 250 15.049’

E 0820 49.125’

-

21

S

No.


collection


14. Udairajpur (Varanasi) 02/09/2015 ELEV: 240 Ft

N 250 17.513’

E 0820 54.582’

-

15. Vishunpur (Varanasi) 02/09/2015 ELEV: 239 Ft

N 250 17.953’

E 0820 55.323’

-

16. Sherpur (Mirzapur) 03/09/2015 ELEV: 235 Ft

N 250 17.954’

E 0820 55.322’

-


N 250 12.052’

E 0830 00.664’

-


N 250 12.057’

E 0830 00.654’

-


N 250 12.097’

E 0830 00.596’

-

20. Sherpur (Mirzapur) 03/09/2015 ELEV:199 Ft

N 250 12.084’

E 0830 00.614’

-


N 250 12.009’

E 0830 00.007’

-


N 250 12.014’

E 0830 00.037’

-


N 250 12.030’

E 0830 00.044’

-


N 250 12.044’

E 0830 00.032’

-

25. Neyamatpur Kala

(Mirzapur)

03/09/2015 ELEV: 360 Ft

N 250 12.073’

E 0820 59.618’

-

26. Neyamatpur Kala 03/09/2015 ELEV: 204 Ft

N 250 12.095’

-

22

S

No.


collection


(Mirzapur) E 0820 59.615’

27. Neyamatpur Kala

(Mirzapur)

03/09/2015 ELEV: 195 Ft

N 250 11.119’

E 0820 58.039’

-

28. Neyamatpur Kala

(Mirzapur)

03/09/2015 ELEV: 324 Ft

N 250 11.243’

E 0820 58.025’

-

29. Dharahara (Mirzapur) 03/09/2015 ELEV: 391 Ft

N 250 06.157’

E 0820 51.705’

-

30. Dharahara (Mirzapur) 03/09/2015 ELEV: 213 Ft

N 250 06.120’

E 0820 51.700’

-

31. Bhurahiya (Mirzapur) 03/09/2015 ELEV: 324 Ft

N 250 06.001’

E 0820 50.466’

-


N 250 06.001’

E 0820 50.466’

-


N 250 05.887’

E 0820 49.161’

-


N 250 05.892’

E 0820 49.160’

-

23

Annexure 3- Pigeonpea disease survey conducted in Madhya Pradesh during 2015-16 (RAKCA-Sehore).

S.No. Farmer Name District/Tehsile/ Village Cultivar Phytophthora blight (%)

1 Rajaram Patel Sehore/Sehore/Kondra chhity TJT 501 13.33

2 Saiyad Sehore/Sehore/Phoolmogra TJT 501 53.33 3 Dharmendra Bhayal Sehore/Ichhawar/chhawar TJT 501 20.67 4 Naresh Yadav Sehore/Rehti/Rehti Local 15.33 5 Ram Chopadia Shore/Asta/Asta JA-4 6.66 6 Vishnu Prashad Shore/Ichhawer/Mongra ram TJT 501 3.33 7 Mahesh Parmar Sajapur/Kalapipal/Pochaner ICP-88039 20.00 8 Kisan Parmar Sajapur/Kalapipal/Pochaner ICP-88039 3.33 9 Kailash Tomar Rajgarh/Talen/Tikariya Lokal 10.00 10 Sudhir Thakur Rajgarh/Talen/Tikariya Lokal 6.66

Annexure 4- Pigeonpea disease survey conducted in Punjab during 2015-16 (PAU-Ludhiana).

Sr.

No.

Visited field Area sown (acres)

under varieties

D.O.S % disease

incidence

PAU 881 AL 201

1 Village Megowal, District Hoshiarpur 2 acre - 21.6.2015 0.5-5

2 Village Bichohi, District Hoshiarpur - 1 acre 25.6.2015 2.5-10

3 Village Sarhala Khurd, District

Hoshiarpur

1 acre 16.6.2015 0.1-5

4 Village Lalwan, District Hoshiarpur 1 acre 22.6.2015 2.5-10

5 Village Shekh Daulat, District Ludhiana 3 acre 7.6.2015 5-10

6 Village Boparai Kalan, District Ludhiana 1.5 acre 10.6.2015 6-10

7 Village Rakba, District Ludhiana 1.5 acre 10.6.2015 2-20

8 Village Issewal Dhaka, District Ludhiana 0.5acre 0.5 acre 25.6.2015 2-15

9 Experiment area, PAU, Ludhiana - 0.5 acre 11.6.15 2-90

24

Annxure 5. Screening of pigeonpea lines against PB at Sehore. Line No. Entries No. Entries Mean PB (%)

1 Check ICP 7119 20.69

2 1 LRG 151 34.10

3 2 WRGE102 11.32

4 Check ICP 7119 17.50

5 3 NTL-30 22.93

6 4 BRG 15-1 27.69

7 Check ICP 7119 26.25

8 5 ICP7119 47.22

9 6 GJP 1401 26.79

10 Check ICP 7119 13.69

11 7 KPL 43 15.38

12 8 BSMR 736 44.44

13 Check ICP 7119 22.73

14 9 SJP102 9.09

15 10 MAL-13(ch) 10.03

16 Check ICP 7119 10.00

17 11 AKTM 10-12 15.38

18 12 CRG 2012-25 31.02

19 Check ICP 7119 28.57

20 13 RVSA 07-31 29.24

21 14 CRG 2012-20 0.00

22 Check ICP 7119 21.43

23 15 AL 2046 3.85

24 16 ASHA 16.67

25 Check ICP 7119 38.33

26 17 RVSA07-22 18.80

27 18 BSMR 853 30.65

28 Check ICP 7119 47.78

29 19 WRGE65 19.09

30 20 GRG 2013 9.09

31 Check ICP 7119 14.68

32 21 WRGE-92 4.17

33 22 NTL-740 30.00

34 Check ICP 7119 3.13

35 23 CRG 2012-30 26.52

36 24 CO6(ch) 5.56

37 Check ICP 7119 5.26

25

Line No. Entries No. Entries Mean PB (%)

38 25 WRGE-92 8.51

39 26 GRG 2013 13.16

40 Check ICP 7119 11.26

41 27 WRGE 256 0.00

42 28 BDW 2008-7 25.83

43 Check ICP 7119 4.17

44 29 PT 705-3-1-1 20.81

45 30 BRG 1 15.56

46 Check ICP 7119 33.70

47 31 BRG 3 0.00

48 32 WRGE 140 3.13

49 Check ICP 7119 3.85

50 33 MA6 42.86

51 34 GJP1406 25.00

52 Check ICP 7119 20.83

53 35 KDVP-1935 0.00

54 36 RPS2007-10 2.86

55 Check ICP 7119 10.83

56 37 SJP-702 14.29

57 38 KPL-44 8.33

58 Check ICP 7119 2.50

59 39 RVSA 07-29 20.54

60 40 BRG 5 8.33

61 Check ICP 7119 12.91

Documents

Half-yearly Progress Report - National Food Security Missionnfsm.gov.in/Project/ICRISAT/2015-16/NFSM-Pigeonpea... · The detailed report of survey is provided in Annexure 2. RAKCA