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Genetic Improvement Of Genetic Improvement Of Castor In the Western Castor In the Western Hemisphere Hemisphere Dick Auld – TTU/ TX AgriLife Dick Auld – TTU/ TX AgriLife Research Research Steve Oswalt – Texas Tech Steve Oswalt – Texas Tech University University Travis Miller – Texas AgriLife Travis Miller – Texas AgriLife Extension Extension Carlos Demant – UNESP/ TTU Carlos Demant – UNESP/ TTU Mauricio Zanotto – UNESP Mauricio Zanotto – UNESP

Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

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Page 1: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Genetic Improvement Of Genetic Improvement Of Castor In the Western Castor In the Western

Hemisphere Hemisphere

Dick Auld – TTU/ TX AgriLife ResearchDick Auld – TTU/ TX AgriLife ResearchSteve Oswalt – Texas Tech UniversitySteve Oswalt – Texas Tech University

Travis Miller – Texas AgriLife ExtensionTravis Miller – Texas AgriLife ExtensionCarlos Demant – UNESP/ TTUCarlos Demant – UNESP/ TTU

Mauricio Zanotto – UNESP (Brazil)Mauricio Zanotto – UNESP (Brazil)Liv Severino – EMBRAPA (Brazil)Liv Severino – EMBRAPA (Brazil)

Alejandro Navas – CORPOICA (Columbia)Alejandro Navas – CORPOICA (Columbia)

Page 2: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Castor Genetic Improvement Castor Genetic Improvement “The Rest of the U.S.A Team” “The Rest of the U.S.A Team”

Brian Baldwin – MSU Brian Baldwin – MSU Tom McKeon – USDA-ARS (Albany)Tom McKeon – USDA-ARS (Albany)Grace Chen – USDA-ARS (Albany)Grace Chen – USDA-ARS (Albany)Calvin Trostle – Texas AgriLife Ext.Calvin Trostle – Texas AgriLife Ext.Mike Foster – Texas AgriLife ResearchMike Foster – Texas AgriLife ResearchTom Kilcer – Cornell ExtensionTom Kilcer – Cornell ExtensionAlan Taylor – Cornell Geneva Ctr.Alan Taylor – Cornell Geneva Ctr.Bill Crosby – Univ. of Windsor (Canada)Bill Crosby – Univ. of Windsor (Canada)

Page 3: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Mamona (Portuguese)Mamona (Portuguese)

A Lady of Dubious CharacterA Lady of Dubious CharacterAnd the Castor PlantAnd the Castor Plant

““A Little Dangerous”A Little Dangerous”

““Slightly Hot Blooded”Slightly Hot Blooded”

““Very Promiscuous”Very Promiscuous”

Page 4: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Mamona - CastorMamona - Castor

““A Little Dangerous”A Little Dangerous”

Ricin ToxinRicin Toxin

““Slightly Hot Blooded”Slightly Hot Blooded”

World Wide in the TropicsWorld Wide in the Tropics

““Very Promiscuous”Very Promiscuous”

Pollinates Over Five MilesPollinates Over Five Miles

Page 5: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Tracking pollen flow in Mississippi USA99.8% of all pollen limited to 6-6.5m

Page 6: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Castor Improvement for Biodiesel & Castor Improvement for Biodiesel & Industrial Oil for All AmericaIndustrial Oil for All America

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Page 7: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Castor - (Castor - (RicinusRicinus communiscommunis L.) L.)

Seed YieldSeed Yield: 1000 to 3,360 kg/ha : 1000 to 3,360 kg/ha Oil ContentOil Content: 45 to 60% (500 to 1,800 l/ha): 45 to 60% (500 to 1,800 l/ha) Fatty AcidsFatty Acids: 85% ricinoleic acid: 85% ricinoleic acid DisadvantagesDisadvantages: Ricin & Allergens: Ricin & Allergens AdvantagesAdvantages: Highest Oil Yields of Any : Highest Oil Yields of Any

Temperately Adapted Oilseed Crop PlantTemperately Adapted Oilseed Crop Plant

Page 8: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Genetic Yield Genetic Yield EnhancementEnhancement

Castor

Future Castor ResearchFuture Castor Research

Reduced Reduced Ricin ToxinRicin ToxinImproved Improved

Water UseWater Use

Page 9: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Conventional Genetics to Conventional Genetics to Reduce Ricin Levels in CastorReduce Ricin Levels in Castor

2003 - Release TTU LRC Germplasm2003 - Release TTU LRC Germplasm Crop Sci 43 : 746-747Crop Sci 43 : 746-747

2009 - Release of 2009 - Release of ‘Brigham’ Castor‘Brigham’ CastorHale = 4,210 ug/ml (38% Total Proteins)Hale = 4,210 ug/ml (38% Total Proteins)Brigham = 416 ug/ml (4% Total Proteins)Brigham = 416 ug/ml (4% Total Proteins)

2012 – Future Castor Varieties2012 – Future Castor VarietiesMcKeon = 40 ug/ml (0.4% Total Proteins)McKeon = 40 ug/ml (0.4% Total Proteins)

Page 10: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Three University CollaborationThree University Collaboration

Tissue cultureTissue culture Elimination of ricin by genetic Elimination of ricin by genetic

transformationtransformation Elimination of the allergensElimination of the allergens Leading to safe castorLeading to safe castor

Page 11: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Dryland Castor Production inDryland Castor Production in North East BrazilNorth East Brazil

Page 12: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Hand HarvestedHand Harvested

Page 13: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Lack of LocalTransportation

Page 14: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Limited Mechanized HarvestLimited Mechanized Harvest

Page 15: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Charter Members of the “Mamona Mafia”

Columbia, U.S., & BrazilColumbia, U.S., & Brazil

Page 16: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Excellent Excellent Drought Drought ToleranceTolerance

Page 17: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Salt ToleranceSalt Tolerance

Page 18: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Hybrid Vigor

Page 19: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Genetic Diversity

Page 20: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Genetic DiversityGenetic Diversity

Page 21: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Broad Adaptation

Page 22: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

North American Castor North American Castor ProductionProduction

High Yielding & Adapted VarietiesHigh Yielding & Adapted Varieties

Drought & Salt Tolerant VarietiesDrought & Salt Tolerant Varieties

Mechanized Production & HarvestingMechanized Production & Harvesting

Cost Effective & Sustainable ProductionCost Effective & Sustainable Production

Page 23: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

1. Semi-dwarf Plant 1. Semi-dwarf Plant Varieties.Varieties.2.2. Apply Plant Growth Regulators.Apply Plant Growth Regulators.3.3. Fully Mechanized Production.Fully Mechanized Production.4.4. Production on Marginal Land.Production on Marginal Land.

“ “ Most Economical Source Most Economical Source of Industrial Oil Feedstocks”of Industrial Oil Feedstocks”

Production of Castor onProduction of Castor onthe Texas High Plains the Texas High Plains

Page 24: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Production Field of ‘Hale’ CastorProduction Field of ‘Hale’ Castor Lubbock, Texas in 2008Lubbock, Texas in 2008

Page 25: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Production Field of ‘Hale’ CastorMemphis, Tennessee in 2006

Page 26: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Combine Harvest ofCombine Harvest of‘‘Hale’ Castor SeedHale’ Castor Seed

Page 27: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Castor Seed in CombineCastor Seed in Combine Prior to CleaningPrior to Cleaning

Clean Seed Yield ExceededClean Seed Yield Exceeded 3,000 kg/ha3,000 kg/ha

Page 28: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

Castor has a Castor has a Promising FuturePromising Future

Page 29: Genetic Improvement Of Castor In the Western Hemisphere Dick Auld – TTU/ TX AgriLife Research Steve Oswalt – Texas Tech University Travis Miller – Texas

AcknowledgementAcknowledgement

Texas AgriLife Research and Texas AgriLife Research and Extension and Texas Tech UniversityExtension and Texas Tech University

Ms. Marjorie KlaymanMs. Marjorie KlaymanAlnor Oil Company, Inc.Alnor Oil Company, Inc.

Dr. Travis Miller – Texas A&MDr. Travis Miller – Texas A&M

Dr. John B. Morris (USDA-ARS)Dr. John B. Morris (USDA-ARS)Griffin, GA (Germplasm)Griffin, GA (Germplasm)