Événement Parlons Affaires Photonics North 2014, Montréal, 29 mai

Événement Parlons AffairesPhotonics North 2014, Montréal, 29 mai

Technologies originating from the research results of

Prof. Denis Boudreau (COPL, Université Laval)

Simple, fast and cost-effective tools for biodiagnostics and medical research

Novel nanobiosensors

Presented by Martin Castonguay, SOVAR

Parlons Affaires Photonics North 2014, Montréal, 29 mai

Technology: “what and why ?”Plasmonic fluorescent core-shell nanoparticles

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Silver core (~50 nm dia.)

Silica shell (~3 nm th.)Molecular probe (no signal)

Target molecule captured by molecular probe (strong signal)

Silica surface for easy immobilization of biomolecules

Silver core for enhanced fluorescence

Strong signalResistance to photobleaching

Excellent signal/noise Versatility

Molecular probe for specificity


Application example #1: PCR-free genotyping

• Phenotyping and immunological methods are gradually being replaced by genotyping methods

• Genotyping methods utilizing PCR amplification of genetic material are costly, complex and time-consuming

• PCR-free cost-effective and timely genotyping tools would increase time-to-answer and decrease complexity and costs

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Technological advantagesDNA sensing based on fluorescent nanoparticles

and a polymeric hybridization transducer

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• Sensitive: 1000 copies, vs. 10 copies for PCR ~10M copies are available from 1 ml of blood

• Rapid: result available <10 min after mixingSimple sample preparation AND no amplification required

• Low cost: straightforward fabrication process and readily available instrumentation for detection


Proof of principleSRY and Dombrock genes were detected directly

from DNA extracted from human blood cells

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Fluo

resc

ence

sig

nal (

a.u.

)

Male donors Female donors Doa (+)

Fluo

resc

ence

sig

nal (

a.u.

)

Doa (-) Dob (+) Dob (-)

SRY genotyping results for 10 non-amplified genomic DNA samples.1

Dombrock (Doa/Dob) genotyping results for 6 non-amplified genomic DNA samples.2

Brouard D, Ratelle O et al. (2013) Analytical Methods 5(24)

Unpublished data (2014).


Preliminary evaluation of the cost per test

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Batch size Projected cost per test

Low scale, using research facilities and personel 6$

Large scale <1$


Application example #2: Functional cell imaging


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Adaptability to different functional fluorophores pH sensibility Calcium imagery Chloride Sodium, Potassium DNA probes

Stable grafting on surfaces Non-invasive measurements Quantitative in vitro

analyses Multi-elementary response Commercial microscopy

hardware

“Fluorescent nanosensors and uses thereof”, Canada/US provisional patent filed May 23, 2014.

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Proof of principleLive extracellular pH measurements on calcified cardiac

fibroblasts directly in the culture medium

pH = 6.5

Substrate fluorescence

Control GFP GFP + CA126.0

6.2

6.4

6.6

6.8

7.0

7.2

Ex

tra

ce

llula

r p

H

Addition of therapeutic drug

Adaptable for various studies• Heart and lung cells (Na+, K+, pH)• Cancer and tumors (pH, Cl-, Ca2+)• Neurons (Ca2+, neurotransmitters)

IP protection

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Patent 1: Polymer transducer

Canada, US, Europe (UK, France, Germany), Australia and Japan

Patent 2: Combination of polymer transducer to fluorescent reporterCanada, US, Europe (UK, France, Germany, Spain, Swiss), Australia and Japan

Patent 3: Combination of polymer transducer and particlesCanada, US.

A patent application was just filed a week ago for the surface-grafted nanoparticles.


Take home message ?

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Fast time-to-answer

Uses existing

detection hardware

Low-cost adaptable product

International market

Multiple applications

Benefits for Canadians

Newborn screening

CancerPharmacogenomics

Pathogen detection


Prof. Boudreau and his team are experts in the field of nanoparticles.

If you are interested in functional nanoparticles for medical or other applications, there may well be a match between his science and your business needs !

[email protected]

Documents

Événement Parlons Affaires Photonics North 2014, Montréal, 29 mai