Applications in cocktail · PDF fileApplications in cocktail production. 2 PerkinElmer Groningen, Netherlands 42 people Production of 3 product groups ... Fatty alkohol ethoxylate

11 © 2009 PerkinElmer© 2009 PerkinElmer© 2009 PerkinElmer© 2009 PerkinElmer

Ronald Edler, Jan ter Wiel

May 2009

Quality Control in LSCApplications in cocktail production

22

PerkinElmer Groningen, NetherlandsPerkinElmer Groningen, Netherlands

42 people

Production of 3 product groups

6 scientists for production support and development (chemistry / biology)

Global distribution

PerkinElmer Groningen… 3 product groupsPerkinElmer Groningen… 3 product groups

33

1. Microplates

� Complete range of standard

microplates

� Clean room processing in

Groningen (FlashPlates,

LumaPlates, barcoding,

custom plate coating)


44

2. Luminescence Reagents

� LITES reagents based on

luciferase reaction

� For reporter gene assays,

cell proliferation, kinase

assays


55

3. LSC Cocktails and Supplies

� Widest range of LSC cocktails with

over 35 years of history:

� Complete range of glass and

plastic vials for counting

� Developed, produced and

supported from Groningen!

Packard BioScience e.g. Ultima Gold range, PicoFluor range

WALLAC e.g. HiSafe range

NEN e.g. Atomlight


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PerkinElmer Groningen… leader in LSCPerkinElmer Groningen… leader in LSC

Product groups:Product groups:

LSC Cocktails and Supplies

Widest range of LSC cocktails with over 35 years of history:

Packard BioScience e.g. Ultima Gold range

WALLAC e.g. HiSafe range

NEN e.g. Atomlight

LUMAC e.g. RiaLuma, AquaLuma

Complete range of glass and plastic vials for counting

Developed, produced and supported from Groningen

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Production of LSC Cocktails

� Cocktail raw materials:

� Incoming QC inspection using for

instance FTIR Spectrum 100

� Testing on colour, LSC

background, efficiency, etc.

Solvents ‘safe’ / ‘classical’ energy transfer

Surfactants many different types sample uptake

Scintillators primary / secondary wavelength shift


88

Production of LSC Cocktails� Dedicated vessels for cocktail preparation

• 400 to 5000 L vessels

• Explosion-safe equipment

• Chemical treatments to improve performance by removing colour, reducing

luminescence, reducing quench


999

LSC Cocktail production/quality

� Final QC inspection – performance (eff., bkgd, sample uptake)

� Retention samples

� Dispensing in 5L cans, bottling in glass

� Storage and worldwide distribution (TNT + Fedex)


1010

A. 40K-contamination of nonylphenolethoxylates (NPE):

• NPE’s are bulk chemicals used for laundry detergents and cleaners

• Low-cost production in a competitive market is required

• As a result, low-cost potassium catalysts are nowadays used in the production of NPE’s

• NPE’s are important components of most LSC cocktails

Ongoing LSC Quality IssuesOngoing LSC Quality Issues

1111

A. 40K-contamination of nonylphenolethoxylates (NPE):

• Quantulus detects these contaminants in Low Level mode (Turku results)

• Ch. 5 – 200 = 3H (+ luminescence) / Ch. 200 – 1024 = 40K

• Careful selection or dedicated production of NPE’s is required

...uantulus\IGLACT1\S






Sample Spectrum

1,000900800700600500400300200100

0.022

0.02

0.019

0.018

0.017

0.015

0.014

0.013

0.012

0.01

0.009

0.008

0.006

0.005

0.004

0.003

0.001

0

-0.001

-0.003

-0.004


1212

B. Environmental ban on the use of nonylphenolethoxylates (NPE):

Endocrine disruptions in Aquatic Environment

� EEC regulations Nonylphenol Ethoxylates (1992)

� European directive 2003/53/EC : level < 0.1 %

USA

� C&EN june 2006, discussion on ban in the US

Environmental Issues of SurfactantsEnvironmental Issues of Surfactants

1313

B. Environmental ban on the use of nonylphenolethoxylates (NPE):

� Reformulation of LSC cocktails to avoid NPE’s is required

� Alcohol ethoxylates are considered as alternative surfactants

� Reformulations ongoing,

� PicoFluor-15

� Focus on efficiency

� background

� Sample load capacity 0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0

Serum

0.1 M Imidazole (pH=7.4)

0.1 M HCl

10 % TCA

0.25 M Amm. Acetate

0.1 M Sodium Sulphate

0.04 M Sodium Phosphate

0.1 M PBS

0.5 M NaOH

0.1 M NaOH

Digoxin

0.05 M Tris/HCl

0.01 M PBS

0.15 M NaCl

water

Pico Fluor 15 Pico Fluor 15 New


1414

C. 14C contamination by chemical components from bio-origin:� Alcohol ethoxylates and other chemicals can be manufactured using fatty

alkohols of natural origin� Availability of bio-chemicals is increasing - cost is reducing� Bio-chemicals contain 14C in contradiction to chemicals from fossil oil

chemical origin

0

5

10

15

20

Nonylphenol

ethoxylate

Fatty alkohol

ethoxylate

Alkohol

ethoxylate

Alkohol

ethoxylate

Synthetic alcohol

ethoxylate

Fatty alkohol

ethoxylate

cp

m

counts in C 14 region

LSC Issues: Renewable Chemical SourcesLSC Issues: Renewable Chemical Sources

1515

LSC : surfactants quality controlLSC : surfactants quality control

QC methodologies / requirements for Nonylphenol Ethoxylates ( NPE’s)

•Color in product

•Counting characteristics in LSC

•Background development with sample

•Salt content in the surfactants

Test Methodology

•20 gram Sample•30 gram Standard Pseudocumene (Pseudocumene, PPO, Bis-MSB)

•Measure in Low level ( TriCarb)

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Background in low level counting mode

Heating Sample 0 - 18.6 0.5 - 5 0 - 156 %Lum

none Cycle 1 4,35 2,53 5,85 20

Cycle 2 4,47 2,63 5,9 15

Cycle 3 4,32 2,32 5,78 16

100 °C Cycle 1 3,87 2,20 5,18 18

Cycle 2 3,92 2,25 5,48 17

Cycle 3 4,00 2,03 5,47 17

150 °C Cycle 1 179,00 173,00 180,00 100

Cycle 2 101,00 99,00 103,00 100

Cycle 3 71,00 69,00 72,00 98

• Heat treatment of Surfactants

• Surfactant low level background after heating at 100 and 150 °C

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Quantulus : Graph of counts observed of different surfactants� Full spectrum

1818


Quantulus : Graph of counts observed of different surfactants� Random signals

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C. Renewable source ethanol� Renewable sources of chemicals available� Main emphasis on ethanol� Ethanol applied in production of chemicals

0

5

10

15

20

25

30

Blank cocktail Ethanol 96 % TEP lot 1000 TEP lot 087 TEP lot 1008 Jenever

counts in C 14 region

cp

m 0

-15

6(L

L)


2020

D. 14C presence in chemical components from bio-origin:� Example of 14C presence in Triethyl phosphate made with bio-ethanol

0

5

10

15

20

nd 1/08114 CHI/29075 CHI/12095 CHI/08066 CHI/08017 CHI/30035 cocktail

cpm samples raw material

cp

m

lot numbers0 – 18.60 – 156


2121

D. 14C presence in chemical components from bio-origin:� Example of 14C presence in Chemicals from bio origin


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PerkinElmer Groningen

...continued support and commitment...continued support and commitment

SUMMARY:SUMMARY:

�� Changes in legislation and the world chemical market requires cChanges in legislation and the world chemical market requires continueing support and efforts in ontinueing support and efforts in

LSC countingLSC counting

�� PerkinElmer continues its focus on LSC Quality PerkinElmer continues its focus on LSC Quality

�� This can only be achieved by continuously monitoring the qualityThis can only be achieved by continuously monitoring the quality and applications of its products and applications of its products

and a proactive approach and a proactive approach

2323

RRD Specialists

Associate Product Leader Associate Product Leader ––

Chuck Passo, Downers GroveChuck Passo, Downers Grove

Tel.: +1Tel.: +1--630630--322322--54005400

Mobile: +1Mobile: +1--815815--483483--18261826

[email protected]@perkinelmer.com

Chuck in the box

Ronald EdlerRonald Edler

European Line Leader Radiometric DetectionEuropean Line Leader Radiometric Detection

Mobil: +49(0)172Mobil: +49(0)172--63859096385909

Email: Email: [email protected]@perkinelmer.com

Documents

Applications in cocktail · PDF fileApplications in cocktail production. 2 PerkinElmer Groningen, Netherlands 42 people Production of 3 product groups ... Fatty alkohol ethoxylate