Acido Salicilico Como Mecanismo de Defenza

7/24/2019 Acido Salicilico Como Mecanismo de Defenza

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Role of Salicylic Acid in Tomato Defense against Cotton Bollworm, Helicoverpa

armigera Hubner

Jinying Peng, Xiaojun Deng, Jianhua Huang, Shihai Jia, Xuexia Miao, and

Yongping Huang*

Institute of Plant Physiology and Ecology, Shanghai Institute fo !iological Sciences,"he #hinese $cade%y of Sciences, &aduate School of the #hinese $cade%y of Sciences,'(( )englin *oad, Shanghai, +((('+, #hina )ax- ./0+1023 4+ 3( 35E0%ail- yongping6ii ssip

pa c cn

* $utho fo coespondence and epint e7uests

8 9atufosch 59 c, .2/:./+ ;+((3ed June +1?$ugust +(, +((3

@e in>estigated the ole of the salicylic acid ;S$< signaling pathAay in defense esponsesof to%ato plants to the heBi>oe, cotton BollAo% $fte exposue to the cotton BollAo%,to%ato lea>es apidly accu%ulated a high le>el of S$ "he tansciption of PR1 and BGL+genes, the %aCe genes of S$ pathAay, Aas up0egulated $n enhanced endogenous S$le>el Aas acco%paniedBy an incease in the endogenous H++ le>el as co%paed Aith con0tols Spaying to%ato plants Aith a solution containing eithe S$ o %ethyl salicylic acid;Me0S$el da%atically inceased "hese data po>ed that the S$ pathAayAas in>ol>ed in the to%ato plant defense esponses to the heBi>oe

Key words: Lycopersicon esculentum, Salicylic $cid PathAay, Plant Defense esponse

Introduction

Plants ae challengedBy a >aiety of aBiotic andBiotic stesses "he diffeential acti>ation of dis0

tinct sets of genes o gene poducts in esponse to

these challenges is efeed to as specificity Se>0

eal signaling pathAays, including jas%onic acid;J$ol>ed plant defense esponses ae

chaacteiFed as species specific E>en in tAo phy0logenetic closely elated plant species such as to0

%ato and toBacco, the S$0dependent defense

pathAay does not tigge the sa%e defense e0

sponses It also %eans that the outco%e of a !"H

;BenFothiodiaFole< teat%ent cannot Be pedicted

and has to Be tested fo each plant0pathogen co%0

Bination

$lthough Both S$ and J$ ae in>ol>ed in plant

defenses, the antagonistic inteactions BetAeen the

S$ and J$ signaling %echanis%s %odulate de0

fense gene expession and the acti>ation of *#Y10

(4'4:2(52?+((3?11((:(.2/ G (/(( H +((3 elag de 8eitschift f 9atufoschung, "Bingen K http-??AA A Fnatufoschco% K D
mailto:[email protected]:[email protected]:[email protected]:[email protected]://www.znaturforsch.com/http://www.znaturforsch.com/mailto:[email protected]


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J Peng et al. K Defense and Salicylic $cid .25

.25 J Peng et al. K Defense and Salicylic $cid

confeed gene0fo0gene esistance to #M;Ye using CnoAn con0

centations of H++ ;Sig%aese tansciption eaction Aas caiedout as eco%%ended By the %anufactue of theCit ;"aCaa (14$, saCa, Japaned in ca0

tepilla0da%aged plants "he >aiation of the S$a%ount Aithin +3 h indicated that the S$ pathAay

esponded stongly to cotton BollAo% feeding

!"pression o# de#ense related genes induced y

cotton ollworm #eeding in tomato seedlings

insect feeding

contol

$tatistical analysis

Statistical analysis Aas pefo%ed By the teat0%ent of one Aay $9$ Aith Posthoc "uCeyHSD tests ;p T ((2


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nda%aged to%ato seedlings shoAed >ey loA

le>els of PR1, BGL+ and P%L %9$ HoAe>e,

in seedlings injued By cotton BollAo%s, the tan0sciption of PR1, BGL+ and P%L Aas stongly in0

duced 1 h afte feeding and Cept inceasing fo ':

/ h $fte / h, tansciption le>els Began to

decease PR1 and BGL+ Aee the co%%on%aCe genes fo the S$ pathAay, Ahich function

doAnstea% in the S$ pathAay ;Yalpani et al.,

1443edin the Biosynthesis of the signal %olecule S$;Mauch0Mani and SlusaenCo, 144/els in plant tissues %ight ha>e a close

positional elationship in the S$ pathAay

)ig + "i%e couse of expession of PR1, BGL+, andP%L genes in esponse to cotton BollAo% feeding "hesa%ples Aee ha>ested (, (2, 1, ', /, 1+ and +3 h aftefeeding "he expession Aas analyFedBy *"0P#* usingtotal 9$ isolated fo% lea>es and specific pi%es fo

PR1, BGL+, and P%L gene a%plifications $s a contol,actin %9$ Aas also a%plified

"i%e UhV

"i%e UhV

)ig ' ;$< #otton BollAo%0induced changes of H++concentation in to%ato seedlings #otton BollAo%sAee inoculated to to%ato seedlings @hen cotton Boll0

Ao%s Began to feed the to%ato lea>es, sa%ples Aeecollected at indicated ti%es Data ae %eans N SE, n ' ;!< #o%paison of the S$ and Me0S$ induced H++dyna%ic patten in to%ato seedlings "he tiangulasy%Bol epesents the S$0teated sa%ple, the s7uaesy%Bol denotes Me0S$0teated sa%ples, and the ciclesy%Bol denotes the Aate0teated sa%ples Data ae ep0esented By %eans N SE, n '

%ccumulation o# H()( induced -y e"ogenous

$% and &e-$%

!ecause S$ and Me0S$ ae consideed to Besignal %olecules that induce a >aiety of plantgenes in>ol>ed in Aound o defense esponses;Senaatna et al., +(((es etuned to the contol le>els

$s co%paed to S$, Me0S$ had a si%ila effecton H++ poduction fo the fist 1+ h HoAe>e, itdiffeed at 1+ h, and etuned to a si%ila le>el at+3 h post0teat%ent "he eason fo this change is


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unCnoAn, But it is possiBly due to the diffeentche%ical stuctues

ose-dependent and time-dependent e##ect o# $%

on the $%-dependent genes e"pression

Spaying to%ato seedlings Aith S$ solutions Be0tAeen (2 and 1( %% the tansciption le>el of se>0

eal doAnstea% co%ponents inceased da%ati0cally in the S$ pathAay ;)ig 3alues N SE Diffeentlettes epesent significantly diffeence at the le>el of

p T ((2;$< Effects of exogenous S$ on the PR1 gene expes0sion Sa%ples Aee ha>ested teated Aith the indicated

concentation at the indicated ti%e, especti>ely Eachgoup epesents a cetain concentation and thee dif0feent ti%e points ;1( %in, '( %in, /( %ine olein the defense esponse to the heBi>oe

Discussion

Pe>ious e>idence has confi%ed that S$ playsa Cey ole in the signal tansduction pathAay lead0ing to syste%ic ac7uied esistance ;S$< ;Enyedi

et al., 144+ol>ed in de0fense esponse to heBi>oe attacCs u esultsde%onstate that the attacC By cotton BollAo%Aould induce the defense esponses of to%ato

plants, including the accu%ulation of H++, the

poduction of S$ and expession of genes acti>ein se>eal steps of the S$ signal pathAay

$n oxidati>e Bust is the fist e>idence of the

S$ pathAay in>ol>ing the to%ato esponse to cot0ton BollAo% feeding ;e>ine et al., 1443= a%Band Dixon, 1445ious studies ha>e de%on0stated that plants infected By pathogens poducean oxidati>eBust and induce the S$ pathAay e0lated esponse theeafte It is easonaBle to hy0

pothesiFe that the S$ pathAay is also in>ol>ed indefense against insect feeding, Because H++ can

da%age the digesti>e syste% of insects and sup0

pess thei de>elop%ent )eeding on pe>iously

Aounded foliage causes oxidati>e da%age in theinsect %id0gut and disupts the aBsoption andtanspot syste%s of heBi>oe insects ;)elton andSu%%es, 144'= )elton et al., 1443els ise shaply in to0

%ato plants in esponse to a cotton BollAo% at0tacC ;)ig '$ol>ed in defense against insect injuy!enFoic acid, the diect suBstate fo S$ Biosyn0

thesis, is dei>ed fo% phenylpopanoid pathAays

in plants Aith P%L as Cey egulatoy enFy%e;Dixon, +((1ol>ed in the pathAayflux contolled By the acti>ity of its ate0contol

enFy%e and suBstate a>ailaBle ;oloso>a et al/,+((1


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of 1 h %ay Be causedBy apid depletion of its suB0state in pool #oesponding to the inceasing ex0

pession of P%L as shoAn in )ig +#, it is sug0gested that Aith a %oe supply of suBstate,

BenFoic acid Aill co%pensate fo a late inceaseof S$ le>el

@e shoAed hee that PR1 and BGL+ genes,acti>e in the S$ pathAay, Aee up0egulated afteda%aged By the cotton BollAo% $phid feedingAas also epoted to induce the acti>ity in the PR1and BGL+ genes in %raidopsis thaliana seedlings;Moan and "ho%pson, +((1ae In contast Aith pathogen o elicitoteat%ent, the induction Aas not appaent foeithe gene Ahen lea>es Aee ha>ested '( %in af0te cotton BollAo% feeding

"he diect e>idence co%es fo% the accu%ula0tion of S$ afte the da%age By the cotton Boll0Ao% $t (2 h afte injuing, the fist peaC of S$

;Panche>a et al., 144/el, suggesting that S$

and Me0S$ acti>ated the poduction of H++In this study, Ae assessed Ahethe the S$ path0

Aay Aas in>ol>ed in defense esponses of to%atoseedlings gaFed By cotton BollAo% la>ae $l0

though Ae did not %easue the acti>ity in the J$

pathAay= the ole of the J$ pathAay in diect plantdefenses against heBi>oes has Been Aell estaB0lished ;i et al., +((+ings

fo linguistic coections of an ealie >esion of

the %anuscipt u thanCs also go to Pof J @

Du and Pof X X 8hu fo thei discussions and

>aluaBle co%%ents "his eseach Aas suppotedBy gants fo% the noAledge Inno>ation Po0ga% of the #hinese $cade%y of Sciences


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!anch #, HAang # ), 9a>ae D $, and @illia%son Mauch0Mani ! and SlusaenCo $ J ;144/oy J #he% Ecol "9, 122':12/. ey%ond P and )a%e E E ;144.oy #u pin Plant !iol ", 3(3:311By Bean leaf and thee coneed alfalfa hoppe J Senaatna ", "ouchell D, !unn ", and Dixon ;+(((a 9, &oenstein 9, ish # M, and Dudae>a sol Plant #ell "5, 5/(:55(

9 ;+((1ine $, "ehnaCen , Dixon , and a%B # ;1443e Bust ochestates the plant saic >ius Plant #ell Physiol '5, .(':.(4hypesensiti>e disease esistance esponse #ell &, "on J, an Pelt J $, an oon #, and Pietese #M3+5:+'5 ;+((+eness of salicylate0depen0

i # Y, @illia%s M M, oh Y ", ee & I, and HoAe dent and jas%onate?ethylene0dependent induced e0& $ ;+((+ated to%ato to cell sistance in %raidopsis. Mol Plant MicoBe Inteactcontent0feeding heBi>oes is egulatedBy the octade0 "5, +5:'3canoid0signaling pathAay Plant Physiol ")(, 343: an PoecCe M P, Posthu%us M $, and DicCe M2(' ;+((1oe0induced >olatile poduction By

MalecC and Dietich $ ;1444ese ene0 toid #otesia uBecula- che%ical, Beha>ioal and gene0%ies[ "ends Plant Sci ', +12:+14 expession analysis J #he% Ecol %#, 1411:14+.

MatineF de Iladuya , Xie \, and aloshian I Yalpani 9, Enyedi $ J, eon J, and asCin I ;1443

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Acido Salicilico Como Mecanismo de Defenza