Seminar Report 2014-15 Protein Chip

ACKNOWLEDGEMENT

I would like to express my sincere gratitude to the principal

Mrs. N. KUMUDINI of KMCT Polytechnic College, Calicut.

I express my deepest sense of gratitude to the Head of the Department of Biomedical

Engineering Mrs. NIRMALA J.A for her valuable advice and guidance.

With immense pleasure and heartiest gratitude, I express my sincere thanks to

Mrs. SHALIMA Lecturer in Biomedical Engineering Department for her valuable

suggestions and guidance.

I also thank to all staff members and dear friends for their kind hearted support and

encouragement.

ABINA SATHYAN

Dept. of Biomedical Engg. KMCT PTC

Seminar Report 2014-15 Protein Chip

ABSTRACT

Protein chips, also referred to as protein arrays or protein microarrays, are modeled

after DNA microarrays. The success of DNA microarrays in large-scale genomic experiments

inspired researchers to develop similar technology to enable large-scale, high-throughput

proteomic experiments. Protein chips enable researchers to quickly and easily survey the

entire proteome of a cell within an organism. They also allow researchers to automate and

parallelize protein experiments.

Protein chips were first developed in 2000 by researchers at Harvard University.

Today there are many companies manufacturing protein chips using many types of techniques

including spotting and gel methods. The types of protein chips available include "lab on a

chip", antibody arrays and antigen arrays, as well as a wide range of chips containing

"alternative capture agents" such as proteins, substrates and nucleic acids.Analysis of protein

chips comes with many challenges including dynamic protein concentrations, the sheer

number of proteins in a cell's proteome, and the understanding of the probes for each protein.

Steps include the reading of the protein levels off the chip, and then the use of computer

software to analyze the massive amounts of data collected.

Applications of protein chip experiments include identifying biomarkers for diseases,

investigating protein-protein interactions, and testing for the presence of antibodies in a

sample. Protein chips have applications in cancer research, medical diagnostics, homeland

security and proteomics.

Dept. of Biomedical Engg. KMCT PTC

Seminar Report 2014-15 Protein Chip

CONTENTS

SL NO: TITLE PAGE NO:

1. INTRODUCTION 1

2. PROTEIN MICROARRAY TECHNOLOGY 2

3. MOTIVATION FOR DEVELOPMENT 3

4. TYPES OF ARRAY 7

5. DETECTION 10

6. APPLICATIONS 11

7. PROTEIN CHIP PRECURSORS TO MODERN DAY 12

8. CHALLENGES 13

9. ADVANTAGES 14

10. CONCLUSION 15

11. REFERENCES 16

Dept. of Biomedical Engg. KMCT PTC