Seminar Report 2014-15 Protein Chip
ACKNOWLEDGEMENT
I would like to express my sincere gratitude to the principal
Mrs. N. KUMUDINI of KMCT Polytechnic College, Calicut.
I express my deepest sense of gratitude to the Head of the Department of Biomedical
Engineering Mrs. NIRMALA J.A for her valuable advice and guidance.
With immense pleasure and heartiest gratitude, I express my sincere thanks to
Mrs. SHALIMA Lecturer in Biomedical Engineering Department for her valuable
suggestions and guidance.
I also thank to all staff members and dear friends for their kind hearted support and
encouragement.
ABINA SATHYAN
Dept. of Biomedical Engg. KMCT PTC
Seminar Report 2014-15 Protein Chip
ABSTRACT
Protein chips, also referred to as protein arrays or protein microarrays, are modeled
after DNA microarrays. The success of DNA microarrays in large-scale genomic experiments
inspired researchers to develop similar technology to enable large-scale, high-throughput
proteomic experiments. Protein chips enable researchers to quickly and easily survey the
entire proteome of a cell within an organism. They also allow researchers to automate and
parallelize protein experiments.
Protein chips were first developed in 2000 by researchers at Harvard University.
Today there are many companies manufacturing protein chips using many types of techniques
including spotting and gel methods. The types of protein chips available include "lab on a
chip", antibody arrays and antigen arrays, as well as a wide range of chips containing
"alternative capture agents" such as proteins, substrates and nucleic acids.Analysis of protein
chips comes with many challenges including dynamic protein concentrations, the sheer
number of proteins in a cell's proteome, and the understanding of the probes for each protein.
Steps include the reading of the protein levels off the chip, and then the use of computer
software to analyze the massive amounts of data collected.
Applications of protein chip experiments include identifying biomarkers for diseases,
investigating protein-protein interactions, and testing for the presence of antibodies in a
sample. Protein chips have applications in cancer research, medical diagnostics, homeland
security and proteomics.
Dept. of Biomedical Engg. KMCT PTC
Seminar Report 2014-15 Protein Chip
CONTENTS
SL NO: TITLE PAGE NO:
1. INTRODUCTION 1
2. PROTEIN MICROARRAY TECHNOLOGY 2
3. MOTIVATION FOR DEVELOPMENT 3
4. TYPES OF ARRAY 7
5. DETECTION 10
6. APPLICATIONS 11
7. PROTEIN CHIP PRECURSORS TO MODERN DAY 12
8. CHALLENGES 13
9. ADVANTAGES 14
10. CONCLUSION 15
11. REFERENCES 16
Dept. of Biomedical Engg. KMCT PTC