"Can you hear that cell ?" - Patch clamp

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Georg Ohm (1789-1854)

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Georg Ohm (1789-1854)

Example with potassium

Bert Sackmann (left) and Erwin Neher (right) Nobel prize for patch-clamp (1991)

In bath : current amplitude is directly dependant of pipette size On cell: current amplitude is directly dependent of seal quality In whole-cell mode: Current amplitude is directly dependent of cell RESISTANCE Current decay (and charge) properties are directly dependent on cell morphology/size

In bath : current amplitude is directly dependant of pipette size On cell: current amplitude is directly dependent of seal quality In whole-cell mode: Current amplitude is directly dependent of cell RESISTANCE Current decay (and charge) properties are directly dependent on cell morphology/size

In bath : current amplitude is directly dependant of pipette size On cell: current amplitude is directly dependent of seal quality In whole-cell mode: Current amplitude is directly dependent of cell RESISTANCE Current decay (and charge) properties are directly dependent on cell morphology/size

In bath : current amplitude is directly dependant of pipette size On cell: current amplitude is directly dependent of seal quality In whole-cell mode: Current amplitude is directly dependent of cell RESISTANCE Current decay (and charge) properties are directly dependent on cell morphology/size

In bath : current amplitude is directly dependant of pipette size On cell: current amplitude is directly dependent of seal quality In whole-cell mode: Current amplitude is directly dependent of cell RESISTANCE Current decay (and charge) properties are directly dependent on cell morphology/size

In bath : current amplitude is directly dependant of pipette size On cell: current amplitude is directly dependent of seal quality In whole-cell mode: Current amplitude is directly dependent of cell RESISTANCE Current decay (and charge) properties are directly dependent on cell morphology/size

In bath : current amplitude is directly dependant of pipette size On cell: current amplitude is directly dependent of seal quality In whole-cell mode: Current amplitude is directly dependent of cell RESISTANCE Current decay (and charge) properties are directly dependent on cell morphology/size

In bath : current amplitude is directly dependant of pipette size On cell: current amplitude is directly dependent of seal quality In whole-cell mode: Current amplitude is directly dependent of cell RESISTANCE Current decay (and charge) properties are directly dependent on cell morphology/size

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