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WTSI Mouse Genetics ProgrammeCASIMIR Meeting, July 2007
The WTSI Mouse Genetics Programme
• 5 year programme funded by the Wellcome Trust (starting Oct 2006)
• Starting at 250 KOs per year
• Targeted mutations in ES cells (from EUCOMM & KOMP)
• Phenotype driven screening programme– Where are the genes expressed and when?– What are the roles of the genes in normal development?– Do the genes play a part in disease?
• All phenotyping will be done on a single genetic background*
• All phenotyping data will be released to WWW after QC
• All biological resources will be available with minimal MTA
+ 4 Year EUMODIC contract - 40 alleles per year (starting Feb 2007)
WTSI Mouse Genetics Central Database
• Genes, Gene builds, existing alleles, external links• Allele pipelines for EUCOMM & KOMP targeting @ WTSI and soon
NORCOMM• Havana annotation pipeline• Automated vector design pipeline (Vivek Iyer)• Allele descriptions• ES Cells • Microinjection pipeline @ WTSI• Cryopreservation pipeline @ WTSI• Mouse tracking• Animal Welfare Sheets• Home Office procedure reporting• Mouse related protocols• Phenotyping data capture• Expression data capture
• Machine integration e.g. genotyping robot, haematology & clinical chemistry
In development or coming soon:
• Biomart and DAS servers– Making KOMP, EUCOMM & NORCOMM production live
• EuroPhenome integration• SOPs
– Automated protocol interface generation– Integration with mouse phenotyping
• Microinjection pipelines for all of EUCOMM• Automated histology image capture (DMetrix DX40 platform)• Data analysis and reporting
External hosting
Live views to external webites• http://komp.sanger.ac.uk• http://www.eucomm.org/• http://www.sanger.ac.uk/Teams/Team109/
What’s needed
• One stop shop for mouse alleles– Existing lines– Production pipeline for KEN– Mouse production for KEN– Links to HTP phenotyping & expression data
• Subject for an FP7 coordinated action– Sanger– EBI– GSF– Jax
Thanks to:
Mouse Informatics GroupDavid MelvinWei LiArthur EvansPaul WestonDavid GannonBhuvan SharmaMark GriffithsChristian KippJohn AmbroseDavid Richardson
T87/EUCOMM Informatics GroupVivek Iyer
MGWGKaren SteelAllan BradleyWilliam SkarnesGordon DouganSeth GrantPentao LiuDavid TannahillDavid AdamsLorraine Everett
Havana GroupJen HarrowMark ThomasAlejandro Mujica
Support from: The Wellcome Trust, EUMODIC, EUCOMM, KOMP
MGP Phenotyping Screen
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