WTSI Mouse Genetics ProgrammeCASIMIR Meeting, July 2007

The WTSI Mouse Genetics Programme

• 5 year programme funded by the Wellcome Trust (starting Oct 2006)

• Starting at 250 KOs per year

• Targeted mutations in ES cells (from EUCOMM & KOMP)

• Phenotype driven screening programme– Where are the genes expressed and when?– What are the roles of the genes in normal development?– Do the genes play a part in disease?

• All phenotyping will be done on a single genetic background*

• All phenotyping data will be released to WWW after QC

• All biological resources will be available with minimal MTA

+ 4 Year EUMODIC contract - 40 alleles per year (starting Feb 2007)

WTSI Mouse Genetics Central Database

• Genes, Gene builds, existing alleles, external links• Allele pipelines for EUCOMM & KOMP targeting @ WTSI and soon

NORCOMM• Havana annotation pipeline• Automated vector design pipeline (Vivek Iyer)• Allele descriptions• ES Cells • Microinjection pipeline @ WTSI• Cryopreservation pipeline @ WTSI• Mouse tracking• Animal Welfare Sheets• Home Office procedure reporting• Mouse related protocols• Phenotyping data capture• Expression data capture

• Machine integration e.g. genotyping robot, haematology & clinical chemistry

In development or coming soon:

• Biomart and DAS servers– Making KOMP, EUCOMM & NORCOMM production live

• EuroPhenome integration• SOPs

– Automated protocol interface generation– Integration with mouse phenotyping

• Microinjection pipelines for all of EUCOMM• Automated histology image capture (DMetrix DX40 platform)• Data analysis and reporting

External hosting

Live views to external webites• http://komp.sanger.ac.uk• http://www.eucomm.org/• http://www.sanger.ac.uk/Teams/Team109/

What’s needed

• One stop shop for mouse alleles– Existing lines– Production pipeline for KEN– Mouse production for KEN– Links to HTP phenotyping & expression data

• Subject for an FP7 coordinated action– Sanger– EBI– GSF– Jax

Thanks to:

Mouse Informatics GroupDavid MelvinWei LiArthur EvansPaul WestonDavid GannonBhuvan SharmaMark GriffithsChristian KippJohn AmbroseDavid Richardson

T87/EUCOMM Informatics GroupVivek Iyer

MGWGKaren SteelAllan BradleyWilliam SkarnesGordon DouganSeth GrantPentao LiuDavid TannahillDavid AdamsLorraine Everett

Havana GroupJen HarrowMark ThomasAlejandro Mujica

Support from: The Wellcome Trust, EUMODIC, EUCOMM, KOMP

MGP Phenotyping Screen