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biosecurity built on science
Detection and surveillance of phylloxera using qPCR
Danièle Giblot-DucraySenior Research Officer, SARDI
Plant Biosecurity Cooperative Research Centre
biosecurity built on science
Problem being addressed
How to find this 1mm aphid…
…in a vineyard, region or country?
Early detection and management of area freedom
Symptoms appear within 3 years with possible vine death within 5-6 years
No treatment, except to replant on resistant rootstocks
Managed by zoning areas (PIZ, PRZ or PEZ) and strict quarantine regulations
Phylloxera is the only regulated pest in the Australian wine industry
biosecurity built on science
Problem being addressed
Sampling - simple and easy Detection - accurate and sensitive Cost effective Scientifically validated peer
reviewed protocol Applicable to other vine
pests/pathogens
Need for high throughput sampling/detection method for
phylloxera
Develop a protocol for phylloxera detection in soil using quantitative PCR (qPCR)
Obtain endorsement and adoption as part of the national standard
Objectives
biosecurity built on science
What are we doing about it?
Applying qPCR for reliable detection of phylloxera in soil samples
Collect composite samples
• using dig stick soil corers
• 0-10cm deep, 5cm from the trunk
• from mid-summer to early winter (higher numbers)
Dry samples within 48h of collection
Sensitivity: 1-2 phylloxera per 200g soil
Establish sampling protocol to give confidence of detection
Compare to existing methods for phylloxera detection
biosecurity built on science
How will this research be delivered?
Endorsement by relevant leadership body
Communication & engagement strategy
Delivery model to industry and regulators
Liaison with national and state regulators Industry meetings at national, state and
regional level Scientific publications/industry journals
Present evidence to national body for endorsement
Method to be integrated into National Phylloxera Management Protocol
Establish innovative delivery model to ensure adoption by wine industry
Support regulators to ensure integration of new method within existing protocols
Continuous improvement Ongoing evaluation Identify areas for future research and
improvement
Oct 2015 –June 2017
June 2016 onwards
July 2016 –June 2017
Dec 2016 –ongoing
biosecurity built on science
Who will benefit from this research?
The collective wine industry Growers Wine producers Contract service providers
Regulators
Industry stakeholders
Researchers
biosecurity built on science
Benefit for our horticultural industries
Allows increased surveillance - Cost effective
- High throughput - Up to 500 samples/day
- Molecular detection from large soil samples (SARDI - Molecular Diagnostics Centre)
Allows early detection and improved quarantine management
Improves management practices and supports business decision making
Drives a risk-based approach to phylloxera management
Provides a tool for- Studying phylloxera biology, movement and vectors
- Detect other pests/pathogens
biosecurity built on science
End-user advocate’s perspectiveA GAME CHANGER “It is easier, quicker and returns better results that give us confidence and knowledge to develop everyday management systems that mitigate the risk of spread”
MALCOM CAMPBELL, fourth generation CAMPBELLS of RUTHERGLEN
biosecurity built on science
Future
Endorsement of method by national leadership body
Inclusion of vine root DNA assessment as quality control
Add value to the method to increase industry adoption
- Assess soil health and other pests and pathogens
Engage growers and determine optimal delivery model to industry and regulators
Case studies to demonstrate value and integrate the tool into existing management strategies
biosecurity built on science
Thank you
PBCRC is established and supported under the Australian Government Cooperative Research Centres Programme
For more information, contact Inca Pearce ([email protected]) Project team
- Ray Correll Rho Environmetrics- Alan McKay SARDI- Kathy Ophel-Keller SARDI- Cassandra Collins The University of Adelaide- Danièle Giblot-Ducray SARDI - Greg King DEDJTR-Vic- Chris Anderson DPI-NSW- Inca Pearce Vinehealth Australia
Department of Economic Development, Jobs, Transport and Resources
