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What was used?Primary antibody: FAST Antibody (STJ93043)
Provider: St John's Laboratory
Dilution ratio: 1:500
Application: Western Blot
Materials for validation: HepG2, HeLa, C6 and NIH3T3 cell lines
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What was the protocol?
Primary Antibody Application 1:500 primary antibody was incubated at 4˚C for overnight.
Secondary Antibody Application Goat secondary antibody at 1:5000 for 1 hour at room temperature.
Visualisation ECL kit. Exposure time 1 min.
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