TNIP2Polyclonal ANTIBODYCatalog Number:15459-1-AP 1 Publications

For Research Use Only

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Basic Information Catalog Number:15459-1-APSize:200 μg/mlSource:RabbitIsotype:IgGPurif ication Method:Antigen aff inity purif icationImmunogen Catalog Number:AG7731

GenBank Accession Number:BC002740GeneID (NCBI):79155Full Name:TNFAIP3 interacting protein 2Calculated MW:49 kDaObserved MW:50 kDa

Recommended Dilutions:WB 1:1000-1:4000 IP 0.5-4.0 ug for IP and 1:500-1:1000 for WBIHC 1:20-1:200 IF 1:10-1:100

Applications Tested Applications:IF, IHC, IP, WB, ELISACited Applications:WBSpecies Specif icity:human,mouse,ratCited Species:mouse

Positive Controls:

WB : HepG2 cells; mouse liver tissue

IP : HepG2 cells;

IHC : human liver tissue;

IF : HepG2 cells;

Background Information TNIP2, also named as ABIN2 and FLIP1, inhibits NF-kappa-B activation by blocking the interaction of RIPK1 with its downstream effector NEMO/IKBKG. Itacts as transcriptional coactivator when translocated to the nucleus. TNIP2 has some isoforms with MW 49 kDa and 37 kDa.

Notable Publications Author Pubmed ID Journal Application

Kate Senger 28420753 Sci Signal WB

Storage Storage:Store at -20ºC. Stable for one year af ter shipment.Storage Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.Aliquoting is unnecessary for -20ºC storage

Selected Validation Data

HepG2 cells were subjected to SDS PAGEfollowed by western blot with 15459-1-AP(TNIP2antibody) at dilution of 1:500 incubated at roomtemperature for 1.5 hours

Immunohistochemical of paraff in-embeddedhuman liver using 15459-1-AP(TNIP2 antibody) atdilution of 1:100 (under 10x lens)

Immunohistochemical of paraff in-embeddedhuman liver using 15459-1-AP(TNIP2 antibody) atdilution of 1:100 (under 40x lens)

Immunofluorescent analysis of HepG2 cells,using TNIP2 antibody 15459-1-AP at 1:25 dilutionand Rhodamine-labeled goat anti-rabbit IgG (red).

IP Result of anti-TNIP2 (IP:15459-1-AP, 4ug;Detection:15459-1-AP 1:500) with HepG2 cellslysate 3000ug.