HOW DID OUR ANTIBODY PERFORM?

CUSTOMER REVIEW: LHX8 Polyclonal Antibody (STJ26197)

WHAT WAS USED?

Primary antibody: LHX8 Polyclonal Antibody (STJ26197)

Provider: St John's Laboratory

Dilution ratio: 1:200

Application: Immunofluorescence

Materials for validation: Lhx8+ cellsFigure 1Tissue section from an E12.5 mouse embryo, ventral telencephalic region.

WHAT WAS THE PROTOCOL?

1. A 7um section of tissue was used.2. Embryos were fixed for 2.5 hours with 4% PFA in PBS.3. They were then incubated in 20% sucrose until they sunk.4. Immersion in cryoprotective solution.5. Flash frozen.6. Blocking solution contained 10% serum, 1% BSA in PBS, 0.5% triton x-100. 7. After O/N incubation with primary antibody, the section was incubated with

secondary antibody (Alexa 488, Invitrogen) for 1 hour. 8. No antigen retrieval was necessary.

N.B. Antigen retrieval procedures with EDTA buffer or Citrate buffer prevented the immunostaining.

WHAT DID THE CUSTOMER THINK?

Testing results were provided by Aldrin Molero from The Saul Korey Department of Neurology stated that “…it works” and “…numerous Lhx8+ cells can be observed in the mantle of the medial ganglionic eminence. These cells correspond to cholinergic progenitor cells”.

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