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Serologia Inmunologia

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Page 1: Serologia Inmunologia

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Page 2: Serologia Inmunologia

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Equipment and infrastructure of diagnostic laboratories for immunology and serology.

Serological diagnosis of viral entities by serum neutralization (SNT).

Viral Identification by virus neutralization (VNT).

Serological diagnosis of viral entities by Hemagglutination / inhibition test (HA-IHA ).

Viral Identification by Hemadsorption test (HAD).

Serological diagnosis by agar gel immunodiffusion (AGID).

Serological diagnosis by passive Hemagglutination (PHA).

Viral identification by counterimmunoelectrophoresis.Identification of emerging microorganisms by immune- electron- microscopy with antibodies colloidal gold marked.

Commissioning and development of ELISA assay for BLV, BHV-1, RHDV, HBHV.

Page 3: Serologia Inmunologia

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Serologic diagnosis of hundreds of entities bacterial, viral and parasitic by ELISA.Commissioning, development and introduction of the immunoperoxidasewith mAbs for the diagnosis of entities bacterial, viral and parasitic.

Commissioning of Mycoplasma sp. culture and serological identification.Use of flow cytometry for quantifying the intracellular interferon-gamma (IFN-γ ) induced by PRRSV.

Detection of interferon-gamma (IFN-γ ) response for swine Tuberculosis diagnosis

Measurement of interferon-gamma (IFN-γ ) ELISA for Bovine Tuberculosis diagnosis.

Multiparameter study of leukocyte subpopulations by flow cytometry.

Cellular cycle. Study by flow cytometry.

Study of different interleukins by flow cytometry and immunofluorescence.

Preparation and evaluation of blood collection cards for serological diagnosis of Encephalitozoon , Chlamydia, Myxomatosis and RHD.

Study of gastroenteritis in domestic animals by Immunochromatography: Astrovirus, Adenovirus, Rotavirus, Coronavirus, Calicivirus and Giardia.

Page 4: Serologia Inmunologia

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Serology of hemoparasites: Elisa for Anaplasma and IFAT for Anaplasma sp., Babesia bovis, B. bigemina, Theileria sp..

Obtaining Riemerella anatipestifer antigen. Protocol for the immunodiffusion test.

Diagnosis of Clostridium botulinum, toxin D. Detection in cell cultures.Serological diagnosis of Leptospirosis by microscopic agglutination test (MAT) with live strains.Serological diagnosis of Leptospirosis by microscopic agglutination test (MAT) with ozone inactivated strains.Serological diagnosis of Leptospirosis by microscopic agglutination test (MAT) with ozone Inactivated strains. Procedures, protocols and routine diagnosis of infectious agentsby immunocytochemistry (direct and indirect immunoperoxidase mAb).

Streptavidin biotin immunoperoxidase in immunocytochemistry.

Neosporosis serological evaluation in sheep / goat by ELISA.

Serological evaluation of acute bovine Neosporosis by avidity ELISA.

Page 5: Serologia Inmunologia

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Laminar antigen processing and evaluation ( IF antigen slide) for BHV-1.

Laminar antigen processing and evaluation ( IF antigen slide) for CpHV-1

Laminar antigen processing and evaluation ( IF antigen slide) for Canine Parvovirus

Laminar antigen processing and evaluation ( IF antigen slide) for Feline Parvovirus

Laminar antigen processing and evaluation ( IF antigen slide) for PRRSV

Laminar antigen processing and evaluation ( IF antigen slide) for BVDV

Laminar antigen processing and evaluation ( IF antigen slide) for Rotavirus type A

Laminar antigen processing and evaluation ( IF antigen slide) for Cryptosporidium sp.

Laminar antigen processing and evaluation ( IF antigen slide) for Leishmania infantum.

Laminar antigen processing and evaluation ( IF antigen slide) for Mixomatosis.

Page 6: Serologia Inmunologia

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Laminar antigen processing and evaluation ( IF antigen slide) for Canine Coronavirus

Laminar antigen processing and evaluation ( IF antigen slide) for Feline Coronavirus

Laminar antigen processing and evaluation ( IF antigen slide) for Feline Calicivirus

Laminar antigen processing and evaluation ( IF antigen slide) for Canine Distemper

Laminar antigen processing and evaluation ( IF antigen slide) for Adenovirus

Laminar antigen processing and evaluation ( IF antigen slide) for Clostridium sp

Laminar antigen processing and evaluation ( IF antigen slide) for Coxiella burnetii

Laminar antigen processing and evaluation ( IF antigen slide) for Babesia bovis

Laminar antigen processing and evaluation ( IF antigen slide) for Theileria annulata

Laminar antigen processing and evaluation ( IF antigen slide) for antinuclear antibodies.

Page 7: Serologia Inmunologia

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Laminar antigen processing and evaluation ( IF antigen slide) for L. monocytogenes.

Diagnosis of Coxiella burnetii by immunofluorescence.

Toxinotypes Clostridium perfringens. Enteotoxemia diagnosis by Dot blot.

Use of flow cytometry to study the antimicrobial susceptibility of Brachyspira sp.

Serological diagnosis of Toxoplasma gondii by direct agglutination and ELISA.Obtaining RHDV antigen for the production of vaccines and for the Hemagglutination inhibition test.

Diagnosis of RHDV by Hemagglutination and capture ELISA.

Serological diagnosis of RHDV by Hemagglutination inhibition and immunofluorescence.

Chemiluminescence for hormone, metabolites and antibodies detection.

Radioimmunoassay for detection of antigens and antibodies.

Virus and protein purification by affinity chromatography and HPLC.