Folding Biología Molecular

PCR newsLAURA MARTÍNEZ CADAVIDSchool of health sciencesSemestre III

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INTRODUCTION

• Diagnosis of influenza is important in treatment

• RIDT’s : Fast and simple to use• PCR: Expensive, results delay

between 1 and 10 days

How Accurate Are Rapid Flu Tests?

ScienceDaily(Feb. 27, 2012)

• PCR allows species identification, using a mitochondrial marker (COI gene) and restriction enzyme assay. This method is low cost and easy to use.

Identifying Sloth Species at a

Genetic LevelScienceDaily(Jan. 6, 2012)

HOW ACCURATE ARE RAPID FLU TESTS?

ScienceDaily. Feb 27, 2012Research has

put the accuracy of

rapid influenza diagnostic

tests

RIDT’ s

Can be used to confirm the flu, but not to rule it

out

Test accuracy is higher in

children than it is in adults

..

More useful in detecting influenza A than influenza B


ScienceDaily. Feb 27, 2012

Gold standars for detecting influenza Can be expensive compared with RIDT’s

The turn-around time for results range from one to 10 days

PCR


ScienceDaily. Feb 27, 2012The study intentionsEarly flu diagnosis is

key for both optimal patient care and infection control.

Implement RIDT’s in the diagnosis of influenza, as a routine implementation.

It is important to note that rapid flu tests can be falsely negative and therefore should not be used to rule out flu.

According to the Public Health Agency of Canada, between 2,000 and 8,000

Canadians can die of the flu and its complications each year, depending on

the severity of the season.


ScienceDaily. Feb 27, 2012

Influenza treatment is most effective when started early in the course of the illness. The ability to start specific influenza therapy sooner in persons at high risk for complications, is a major benefit of these tests

PERSONAL COMMENT

It’s important for us as a sudents to

know all the implements that we have in the moment of the diagnosis. This

type of researches are necesary in the

aplication of the medicine.

The benefits that have

each test and which is more useful as to

price, specificity,

giving results and facilitate treatment.

Identifying Sloth Species at a Genetic Level

ScienceDaily. Jan 6, 2012

IMPORTANT

Identifying species

Separating out each specie

Managin each type on its own

FOR Any animal management system



Species identification of two toed sloths

has been problematic

NEW MOLECULAR TOOL



PCR-based technique

Allows species identification wothout requiring sequencingUsing a mitochondrial marker (COI gene)

Restriction enzyme assay



Patterns of chromosome

variation in the two toed sloths CHROMOSOMES NUMBER 50-

51

LOW VARIATION

PERSONAL COMMENT

Is so importan in the improvement of science. The identification of species is necesary to put it into a taxonomy line

and do and objective observation of them.

MEDICAL UTILITY

Timely diagnosis

Succesful treatment

Provide timely

information

Improve prevention/promotion

Know more about the

disease RIDT’s

Reduce morbidity and mortality of influenza

MEDICAL UTILITY

PCR – RIDT’s

MEDICAL UTILITY

Is important to know all the

new molecular tools and tests

tha help the doctor in the monitoring of the disease

MEDICAL UTILITY

Identification of species is

important to know what type of treatment is appropriate and the clinic of the

disease

BIBLIOGRAPHY

• Science Daily. How Accurate Are Rapid Flu Tests, Study Finds [internet].[S.C]: Science Daily; February 21th 2012[Access day: March 3th 2012]. Disposable in:

http://www.sciencedaily.com/releases/2012/02/120227204725.htm

• Science Daily. Identifying Sloth Species at a Genetic Level, Study Finds [internet].[S.C]: Science Daily; January 6th 2012[Access day: March 3th 2012]. Disposable in:

http://www.sciencedaily.com/releases/2012/01/120106110209.htm

Health & Medicine

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