DNA REPLICATIONLaura Restrepo Carvajal

Molecular Biology Teacher: Lina MartínezMedicine UPB-MedellinAugust 13/2012

In the replication one strand is synthesized continuously and is called the leading strand, the other is synthesizes discontinue from small fragments called Okasaki fragments and RNA using primers

the origin of replication site is a short repeated sequences that is recognized by proteins binding to sites of origin

DNA polymerases synthesize DNA only in the direction 5 to 3, adding a dNTP to the group 3 hydroxyl a emerging chain

Protein involved in DNA replication centrosome regulation liked to Dwafism and small brain size

Protein involved in DNA replication centrosome regulation linked to Dwafism and small brain size

Other roles for ORC, includes centrosome regulation and in that secuence , ORC can be a regulator of the cromosomic division , cause centrosome ensures that the duplicated chromosomes are segregated evenly to the daughter cells.

Protein involved in DNA replication centrosome regulation linked to Dwafism and small brain size

MGS patients often have mutations in the gene encoding the ORC therefore does not prevent the centrosome duplication several times.

Protein involved in DNA replication centrosome regulation linked to Dwafism and small brain

size

The centrosome also plays a role in establishing cell outgrowths, such as axons in brain cells and cilia in many other cells of the body.

Observation

Early detection of mutation of genes encoding the ORC could give more possibilities of treatment to people suffering from the MGS.

New England Biolabs develop novel polymerase with ultra –high fidelity and minimal GS bias

New England Biolabs develop novel polymerase with ultra –high fidelity and minimal GS bias

High fidelity amplification is essential for experiments whose outcome depends upon the correct DNA sequence, such as sequencing, SNP detection, protein expression and gene function studies.

New England Biolabs develop novel polymerase with ultra –high fidelity and minimal GS bias

Q5 High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (>50 times higher than Taq) that is frequently used in polymerase chain reaction (PCR)

New England Biolabs develop novel polymerase with ultra –high fidelity and minimal GS bias

A hot start version of the Q5 polymerase, which utilizes a synthetic aptamer for room-temperature set up, is also available.

Observation

the uniform amplification of sequences, with robustness, coverage, high speed , and a great amplicon length allows the efficiency of the PCR technique

Medical Utility

Medical Utility

Survival of an organism depends on the accurate replication of its genome, an error in the synthesis generates a permanent mutation and the possible elimination of the cell line

Medical Utility

This research has significant implications for processes that control body and brain size; how tissues come to be the size that they are is a little understood area of biology. Studying how mutations in centrosome biology affect tissue and body size may lead to insights into this regulation, Stillman suggests

Medical Utility

These new types of PCR award according to their characteristics, greater certainty to the diagnosis of malignant diseases such as leukemia and lymphomas ,as will be made much easier and faster identification of virus or bacteria causing a disease like SIDA, identify persons (bodies) or do scientific research on DNA amplified

Bibliography

• MARTINEZ SANCHEZ, Lina María. Biología molecular.7.ed.Medellín: UPB.Fac.de Medicina,2012.74-84p

• New England Biolabs Inc. (2012, 07). neb.com. Retrieved 08 09, 2012, from http://www.neb.com: http://www.neb.com/nebecomm/tech_reference/polymerases/Q5.asp#.UCbY0Z3v-TI

• Biosciencetechnology. (2012, 08 1). Retrieved 08 2, 2012, from biosciencetechnology.com: www.biosciencetechnology.com/News/2012/08/Protein-Involved-in-DNA-Replication,-Centrosome-Regulation-Linked-to-Dwarfism,-Small-Brain-Size/

LA VIDA ES AQUELLO QUE TE VA SUCEDIENDO

MIENTRAS TE EMPEÑAS EN HACER OTROS PLANES.

John Lennon