Circulating Cell-Free DNA and

Preeclampsia: Hope For Patients With

HELLP?Chukwuma I. Onyeije, M.D.

http://preeclampsiaonline.net

STUDY:Relationship of circulating cell-free DNA levels to cell-free fetal

DNA levels, clinical characteristics and laboratory parameters in preeclampsia

AUTHORSLevente Lazar , Janos Rigo Jr , Balint Nagy , Krisztian Balogh ,

Veronika Mako , Laszlo Cervenak , Miklos Mezes , Zoltan Prohaszka and Attila Molvarec

CITATION:

BMC Medical Genetics 2009, 10:120doi:10.1186/1471-2350-10-

120Published: 21 November 2009

Study Question: Is cell-free DNA are

related to preeclampsia?

What is Cell-Free DNA?

DNA is nucleic acid that contains the genetic

instructions used in the development and

functioning of all known living organisms.

DNA is normally found in an inner portion of the cell

known as the nucleus.

Recent studies have looked at the importance of so-

called "Cell-free" DNA in various disease states.

Cell-free DNA is DNA that can be detected in circulating blood.

Numerous reports in the literature suggest that levels cell-free DNA in the blood may be a more certain tumors in the detection of early stages of cancer .

Levels of cell-free DNA are also potentially an important marker for pregnancies at risk for premature birth

The current study looks at whether or not cell-free DNA levels in pregnant patients are related to:

the clinical characteristics of preeclampsiathe standard laboratory tests seen in patients with preeclampsiamarkers of inflammation,endothelial activation or injury (in other words injury to the innerlayer of blood vessels often seen in preeclampsia)oxidative stress andcell-free fetal DNA levels

Study Design:

➲ Blood samples of 67 women with preeclampsia were compared to 70 pregnant women without preeclampsia.

➲The analysis looked at levels of cell- free DNA as well as standard laboratory parameters tests obtained in pregnant patients.

Conclusions

Cell-free maternal and fetal DNA were higher in mothers with preeclampsia as shown below:

The quantity of cell-free DNA did not correlate to other lab findings in

preeclampsia (with the exception of liver enzyme changes) and did not

correlate to the clinical characteristics in the mothers (such as body mass

index).

The authors conclude that the increased levels of cell free DNA may be triggered by low-level liver damage seen in patients with preeclampsia.

What does this mean for patients?

Currently, there is not enough support for analyzing cell-free DNA in most patients with preeclampsia.

However, we appear to be on the cusp of a number of technological breakthroughs involving DNA based diagnostic tests which will allow us to make much earlier diagnoses.

In current day medicine, HELLP syndrome is the most severe form of preeclampsia. (HELLP stands for Hemolysis, Elevated Liver enxymes, and Low Platelets).

In order for this diagnosis to be made, the patient's preeclampsia must get to

the point where the liver cells are severely damaged and leaking

enzymes.

The results of the current study provide hope that we may be close to a point where an analysis of cell-free DNA can give information about early liver damage in patients with preeclampsia before they get to the point of having HELLP syndrome.

A full copy of the study can be found here:

http://www.biomedcentral.com/content/pdf/1471-2350-10-120.pdf

Further reading

http://thedecisiontree.com/blog/

The Decision Tree: Taking Control of Your Health in the New Era of

Personalized Medicine

The Future of Medicine: Megatrends in Health Care That Will Improve Your Quality of Life

Genomic and Personalized Medicine,