Welcome to my presentation

Name:

Hasan Al Banna

Exam Roll: 1513

Reg. No.: Ha- 2515

Paper Title:

Habitual sleep duration is associated with BMI and macronutrient intake,

and may be modified by CLOCKgenetic variants

Name of Journal:

The American Journal of Clinical Nutrition

Publishing Date: First online published November 26, 2014,

Present edition: Am J Clin Nutr January 2015 vol. 101 no. 1 page 135-143

Authors: Hassan S Dashti, Jack L Follis, Caren E Smith, Toshiko Tanaka, Brian E Cade, Daniel J Gottlieb, Adela Hruby, Paul F Jacques, Stefania Lamon-Fava, Kris Richardson, Richa Saxena, Frank AJL Scheer, Leena Kovanen, Traci M Bartz, Mia-Maria Perälä, Anna Jonsson, Alexis C Frazier-Wood, Ioanna PanagiotaKalafati, Vera Mikkilä, Timo Partonen, Rozenn N Lemaitre, Jari Lahti, Dena G Hernandez, Ulla Toft, W Craig Johnson, Stavroula Kanoni, Olli T Raitakari, Markus Perola, Bruce M Psaty, Luigi Ferrucci, Niels Grarup, Heather M Highland, Loukianos Rallidis, Mika Kähönen, Aki S Havulinna, David S Siscovick, KatriRäikkönen, Torben Jørgensen, Jerome I Rotter, Panos Deloukas, Jorma SA Viikari, Dariush Mozaffarian, Allan Linneberg, Ilkka Seppälä, Torben Hansen, Veikko Salomaa, Sina A Gharib, Johan G Eriksson, Stefania Bandinelli, OlufPedersen, Stephen S Rich, George Dedoussis, Terho Lehtimäki, José M Ordovás.

Importance of the study

Public Health Concern

Obesity

Hypertension

Cardiovascular disease

Mortality

Diabetes

Short Sleep

Duration

Importance of the study (Continued…)

Increase total energy intake and

risk of obesity….

Influence appetite related

hormone

Sleep Restriction

Importance of the study (Continued……..)

But…..We didn’t know the answer that•Whether chronic metabolic

abnormalities associated with short sleep duration

•Whether CLOCK variants modify the association between habitual short

sleep duration and relative macronutrient intake

What was the objective of the study?

Two basic objective the study:

• Examining associations between habitual sleep duration, body mass index (BMI), and macronutrient intake

a

• Assessing whether CLOCKvariants modify these associations.

b

Methods used in the study

• For habitual dietary intake data collection:

Food Frequency Questionnaires (FFQ)

o interviewer-administered FFQ

o self-administered FFQ

o modified-Block FFQ

• Assessment methods for BMI calculation:

Calculated from measured ratio fromweight (kg) / height (m)2

Methods used in the study (Continued……..)

• For Sleep duration data collection:

By self-reported responses to questions

• For tSNP selection

The web-based service of the Tagger option within Haploview Software

• For testing heterogeneity across studies

Cochran’s Q statistic and quantified by using the I2 statistic

Uniform analysis plan…

Associations between sleep duration and BMI were estimated by using linear regression models

Investigated of associations between CLOCK tSNPs and dietary intake were by using linear fixed-effect

regression models or linear mixed-effects models

Investigation of the sleep duration and tSNP effect modification on dietary intake

Sample Collection• 14,906 participants of European descent from

9 cohort studies of the CHARGE Consortium Nutrition Working Group

•Participants provided written informed consent

•The protocol was approved by local institutional review boards and oversight committees.

Result Analysis

Continued……..

Continued……..

Summary of Result

Identified a significant association between sleep duration and BMI

Associations between sleep duration and intake tended to be age- and sex-specific

CLOCK variants could modify the associations between sleep duration and dietary intake.

Geographic location could have influenced these associations, particularly between CLOCK and PUFA.

Prospects for future study

Geographic Difference

Relevant to Chronic Disease

Potential Interaction Elsewhere

Other dimension

of sleep

Limitation of the Study

Interactions that met pre-specified criteria

Limited to individuals

of European descent

Self-reported habitual sleep duration

The temporal

relation or the causal pathway linking CLOCK,

sleep, and diet.

Concluding remarks

One of the largest observational

study

Public health implications

personalized sleep recommendations

Thanks to all……Wish You All The Best

Hasan Al Banna

Exam Roll: 1513

Reg. No.: Ha- 2515

Department of Biochemistry and Molecular Biology

University of Dhaka