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A presentation about the recent developments in Rapid microbiological detection methods from LumiByte. MuScan for instant single cell detection and the colony tracker for ultra fast antibiotic suseptibility testing. Presented at the Health and Technology event in Papendal the netherlands september 2013
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Accelerating Rapid Diagnostics
Vivian Monteban, September 2013
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LumiByte
We catch microbesaccelerating rapid diagnostics
ultra fast automated optical bacterial detection
Saving time = Saving lives
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Clinical microbiology
• Shorten time till definitive treatment will:
– Decreased morbidity & mortality– Decreased costs – • decreased use of drugs, • the need for isolation, and • the use of additional laboratory and other
diagnostic studies.
– Shorter duration of stay in hospital
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Traditional workflow
Sample collected
Microscopy (often uninformative for
clinical purposes)
Pathogen(s) cultures
Antibiotic sensitivity determined
(complex resistances may need further confirmation)
Empirical therapy
Definitive therapy
Minutes to hours
24-48 hours*
24-48 hours*
*longer for slow growers e.g. Mycobacterium spp.
•Total time: more then 2 days!•Amplification increases error•Can be difficult to take samples 100% sterile
•Speed up individual stages•Combine•Improve Emperical therapy•Shorten time till definitive treatment
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Sample collected
Sample enrichment/Culturing
Antibiotic suseptibility determined
(complex resistances may need even further confirmation)
Day(s) to weeks*
Day(s) to weeks*
Report for QC
Therapy
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D a y ( s ) t o w e e k s *
Day(s) to weeks*
D a y ( s ) t o w e e k s *
Day(s) to weeks*Report for QC
Report for QC
Antibiotic suseptibility determined
( c o m p le x r e s is t a n c e s m a y n e e d
e v e n f u r t h e r c o n fi r m a ti o n )
Antibiotic suseptibility determined
(complex resistances may need even further confirmation)
Sample enrichment/Culturing
Sample enrichment/Culturing
Sample collected Minutes to hours Report for QCTherapy 2-3 timepoints; 1 day
Therapy
Therapy
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Portfolio
• MuScan – single cell detection• Colony Tracker– growth detection (R&D)
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Rapid Detection method
Filtrate Scanning Analyse
10 -60 minutes
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MuScan – single cell detection
Applications
• Process monitoring• Root cause analysis• Hygiene screening and absence
testing (e.g. endoscopes)
Sample size
• Pure water: >1L• Blood : currently 1ml
White light
Blue light Amber lightViolet light
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Key facts (WHO)
• Infections caused by resistant microorganisms often fail to respond to conventional treatment, resulting in prolonged illness, greater risk of death and higher costs.
• A high percentage of hospital-acquired infections are caused by highly resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) or multidrug-resistant Gram-negative bacteria.
• New resistance mechanisms have emerged, making the latest generation of antibiotics virtually ineffective.
Antimicrobial resistanceFact sheet N°194Updated May 2013
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Emergence and spread of AMR (WHO)
Important factors that accelerate the emergence and spread of AMR:
• weak or absent antimicrobial resistance surveillance and monitoring systems;
• inappropriate use of antimicrobial medicines.• insufficient diagnostic, prevention and therapeutic tools.• lack of a comprehensive and coordinated response;
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Portfolio
• MuScan – single cell detection• Colony Tracker– growth detection (R&D)
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Colony Tracker
The Consept: • Monitoring individual bacterial micro-colony growth• Growth based, ultra fast• Antibiotic susceptibility testing• Double infections easily recognized
Simplified image analysisonly colonies grow!
Images provided by: Alice den Hertog - KIT Biomedical Research
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KIT - Tuberculosis
Images provided by: Alice den Hertog - KIT Biomedical Research
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KIT - Tuberculosis
• Conditions can be changed after initial growth detection
Images provided by: Alice den Hertog - KIT Biomedical Research
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Emergence and spread of AMR (WHO)
Important factors that accelerate the emergence and spread of AMR:
• weak or absent antimicrobial resistance surveillance and monitoring systems;• inappropriate use of antimicrobial medicines.• insufficient diagnostic, prevention and therapeutic tools.• lack of a comprehensive and coordinated response;
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The Colony Tracker provides answers days before standard methods!
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Acknowledgment
– Alice den Hertog - KIT Biomedical Research– Richard Anthony - KIT Biomedical Research– Frank Fey – Center for Consepts in
Mechatronics, Nuenen– Colin J. Ingham – MicroDISH, Houten
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Accelerating Rapid Diagnostics
LumiByte B.V.De Pinckart 24Postbus 125670 AA NuenenTel. +31 (0)88 1168600
www.lumibyte.eu
