Accelerating Rapid Diagnostics
Vivian Monteban, September 2013
10 apr 2023 2
LumiByte
We catch microbesaccelerating rapid diagnostics
ultra fast automated optical bacterial detection
Saving time = Saving lives
10 apr 2023 3
Clinical microbiology
• Shorten time till definitive treatment will:
– Decreased morbidity & mortality– Decreased costs – • decreased use of drugs, • the need for isolation, and • the use of additional laboratory and other
diagnostic studies.
– Shorter duration of stay in hospital
10 apr 2023 4
Traditional workflow
Sample collected
Microscopy (often uninformative for
clinical purposes)
Pathogen(s) cultures
Antibiotic sensitivity determined
(complex resistances may need further confirmation)
Empirical therapy
Definitive therapy
Minutes to hours
24-48 hours*
24-48 hours*
*longer for slow growers e.g. Mycobacterium spp.
•Total time: more then 2 days!•Amplification increases error•Can be difficult to take samples 100% sterile
•Speed up individual stages•Combine•Improve Emperical therapy•Shorten time till definitive treatment
10 apr 2023 5
Sample collected
Sample enrichment/Culturing
Antibiotic suseptibility determined
(complex resistances may need even further confirmation)
Day(s) to weeks*
Day(s) to weeks*
Report for QC
Therapy
10 apr 2023 6
D a y ( s ) t o w e e k s *
Day(s) to weeks*
D a y ( s ) t o w e e k s *
Day(s) to weeks*Report for QC
Report for QC
Antibiotic suseptibility determined
( c o m p le x r e s is t a n c e s m a y n e e d
e v e n f u r t h e r c o n fi r m a ti o n )
Antibiotic suseptibility determined
(complex resistances may need even further confirmation)
Sample enrichment/Culturing
Sample enrichment/Culturing
Sample collected Minutes to hours Report for QCTherapy 2-3 timepoints; 1 day
Therapy
Therapy
10 apr 2023 7
Portfolio
• MuScan – single cell detection• Colony Tracker– growth detection (R&D)
10 apr 2023 8
Rapid Detection method
Filtrate Scanning Analyse
10 -60 minutes
10 apr 2023 9
MuScan – single cell detection
Applications
• Process monitoring• Root cause analysis• Hygiene screening and absence
testing (e.g. endoscopes)
Sample size
• Pure water: >1L• Blood : currently 1ml
White light
Blue light Amber lightViolet light
10 apr 2023 10
Key facts (WHO)
• Infections caused by resistant microorganisms often fail to respond to conventional treatment, resulting in prolonged illness, greater risk of death and higher costs.
• A high percentage of hospital-acquired infections are caused by highly resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) or multidrug-resistant Gram-negative bacteria.
• New resistance mechanisms have emerged, making the latest generation of antibiotics virtually ineffective.
Antimicrobial resistanceFact sheet N°194Updated May 2013
10 apr 2023 11
Emergence and spread of AMR (WHO)
Important factors that accelerate the emergence and spread of AMR:
• weak or absent antimicrobial resistance surveillance and monitoring systems;
• inappropriate use of antimicrobial medicines.• insufficient diagnostic, prevention and therapeutic tools.• lack of a comprehensive and coordinated response;
10 apr 2023 12
Portfolio
• MuScan – single cell detection• Colony Tracker– growth detection (R&D)
10 apr 2023 13
Colony Tracker
The Consept: • Monitoring individual bacterial micro-colony growth• Growth based, ultra fast• Antibiotic susceptibility testing• Double infections easily recognized
Simplified image analysisonly colonies grow!
Images provided by: Alice den Hertog - KIT Biomedical Research
10 apr 2023 14
KIT - Tuberculosis
Images provided by: Alice den Hertog - KIT Biomedical Research
10 apr 2023 15
KIT - Tuberculosis
• Conditions can be changed after initial growth detection
Images provided by: Alice den Hertog - KIT Biomedical Research
10 apr 2023
Emergence and spread of AMR (WHO)
Important factors that accelerate the emergence and spread of AMR:
• weak or absent antimicrobial resistance surveillance and monitoring systems;• inappropriate use of antimicrobial medicines.• insufficient diagnostic, prevention and therapeutic tools.• lack of a comprehensive and coordinated response;
16
The Colony Tracker provides answers days before standard methods!
10 apr 2023 17
Acknowledgment
– Alice den Hertog - KIT Biomedical Research– Richard Anthony - KIT Biomedical Research– Frank Fey – Center for Consepts in
Mechatronics, Nuenen– Colin J. Ingham – MicroDISH, Houten
10 apr 2023
Accelerating Rapid Diagnostics
LumiByte B.V.De Pinckart 24Postbus 125670 AA NuenenTel. +31 (0)88 1168600
www.lumibyte.eu
Recommended
