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Cell & Molecular
Biology
Enzymeand Generally
ProteinRegulation
Chapters 3 & 4
EnzymeKinetics
Turnover Number
“...how rapidlythe substratecan be processed.”
Figure 3-26a Essential Cell Biology (© Garland Science 2010)
Figure 3-26b Essential Cell Biology (© Garland Science 2010)
Figure 3-24 Essential Cell Biology (© Garland Science 2010)
Enzymes and substratesencounter each otherthrough diffusion.
Figure 3-22 Essential Cell Biology (© Garland Science 2010)
Inhibitors
http://www.pdb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb45_1.html
Estrogen receptor with DNA and estradiol bound
http://www.pdb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb45_1.html
Tamoxifenblocksthe actionof estrogen
CompetitiveInhibition
Figure 3-28a Essential Cell Biology (© Garland Science 2010)
Figure 3-28b Essential Cell Biology (© Garland Science 2010)
h"p://cnx.org/content/m11456/latest/Trypsin
Benzamidine
NoncompetitiveInhibition
FeedbackInhibition
Figure 4-34 Essential Cell Biology (© Garland Science 2010)
NegativeRegulation
Figure 4-35 Essential Cell Biology (© Garland Science 2010)
Figure 4-36 Essential Cell Biology (© Garland Science 2010)
AllostericRegulation
ProteinPhosphorylation
The reversible additionof a phosphate groupto a protein.
Figure 4-38a Essential Cell Biology (© Garland Science 2010)
Figure 4-38b Essential Cell Biology (© Garland Science 2010)
GTP Binding
Figure 4-39 Essential Cell Biology (© Garland Science 2010)
http://www.rcsb.org/pdb/101/motm.do?momID=81
Elongation FactorEF-Tu
tRNA