Office of the Vice Chancellor for Research
New Faculty Orientation
Office ofResearchServices (ORS)
Office of Research Services &Office of Research Services &Research Development Services Research Development Services
Helping you navigate your research portfolio
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Total DollarsAwarded – FY 2008- FY 2012
0
50
100
150
200
250
300
350
400
450
2008 2009 2010 2011 2012
Mil
lio
ns
*2010 includes ARRA awards
Office of Research Services (ORS)
•Research Development•Proposal and award processing
• Pre-award• Post award (non financial)
•Budget review• Fit with scope
• Statutory, fiscal compliance
•All grant submissions• Sponsors: Government,
industry, foundations, etc.• Modes: Grants.gov, Proposal
Central, paper, etc.
•All grant and contract approvals
• All negotiations • Other agreements
• Material transfer• Professional service
agreements
•Standard information•Sponsor policy updates• Procedural guidance
Research Development Tools, Resources, and Processes
•Searchable Databases•Limited Competitions•Internal Funding
Finding FundingFinding Funding
Resources to find grants matching your research interests
Examples:• Pivot-COS• Grants.gov• Grants Resource Center (GRC)• IRIS• FedBizOpps
Pivot-COSPivot-COS
Pivot - Community of Science• All disciplines, all sponsor types,
international• Save multiple, customized funding alerts• Track opportunities• Matching function• Find collaborators
http://pivot.cos.com/
GRCGRC
Grants Resource Center• Maintains own database and publications that
cover all relevant federal grants and many private grants
• Maintains a library of funded proposals• Connect faculty members with program officers
www.aascu.org/grc (login: uic, pswd: awards)
Limited Competitions
• Sponsor makes university prescreen and select proposal
• Peer reviewed – typically evaluated for: • Program Compliance• Scientific Merit• Management
• Education/Training • MUST have prior authorization to
submit
Limited Competition Process
1. Announcement2. Letter of Intent3. White Papers (if necessary) 4. Peer Review ( if necessary)5. Authorization6. Feedback (if warranted)
This process typically takes from 2 to 4 weeks
Campus Research Board Campus Research Board Pilot Grants Pilot Grants
Up to $20,000 over 1 year Purpose:
• Foster pilot research• Nurture career trajectories of promising early-stage
investigators• Leverage extramural funding
Eligibility: • FT tenure or non-tenure track faculty • Within 8 years of terminal degree
Fall Deadline: • LOI: late summer, 2013 • Application: Autumn, 2013
http://tigger.uic.edu/depts/ovcr/research/funding/CRB.html#eligibility
Chancellor’s Discovery Fund Chancellor’s Discovery Fund for Multidisciplinary Research for Multidisciplinary Research
Up to $40,000 over 2 years Purpose:
• Foster pilot research using the expertise of varied disciplines• Nurture career trajectories of promising early-stage
investigators• Leverage extramural funding• Encourage new or support early stage collaborations
Eligibility: • FT tenure or non-tenure track faculty • Minimum of two Principal Investigators• At least one PI must be within 8 years of their first faculty
appointment FY 13 Deadline:
• LOI: Fall, 2013 (date TBD)• Application: January, 2013
http://tigger.uic.edu/depts/ovcr/research/funding/discovery/
Bridge Funding ProgramBridge Funding Program
Purpose: short term support to sustain research programs, laboratories, and momentum of productive researchers during funding gaps to give them a chance to regain extramural funding.
Eligibility • Full time (at least .8 FTE) senior faculty. Preference will
be given to Associate Professors • Must have been PI on a grant funded at least three
consecutive years (the same grant or project) by a national agency with a bona fide scientific peer-review process (e.g. NSF, NIH, AHA)
Awards: Vary by Phase• 20K for one year or less. Must be matched 1:1
Next deadline: TBD
http://tigger.uic.edu/depts/ovcr/research/funding/Seed_Funding/ovcr_bridge.html#awards
Ignite Proposal Development Ignite Proposal Development ProgramProgram Purpose: Spark proposal planning or
development process Eligibility
• Lead PI must be tenured or tenure track faculty and have a full-time (at least .8 FTE) UIC appointment
• Project must involve 3 or more colleges. Awards: Vary by Phase
• 20K for a six month term AY 2013 deadlines: TBD
http://tigger.uic.edu/depts/ovcr/research/funding/ignite/
Office of Research Services Office of Research Services (ORS)(ORS) Supports faculty from development to
award stage
Authorized institutional representative • Official institutional endorsements• Authorizes all submissions • Accepts all awards• Negotiates all contracts • Government, for profit industry, not for profit
foundations
Central point of coordination for all sponsored projects for the Chicago, Rockford and Peoria campuses
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Internal Approvals: Cross your T, Dot your I, CYA
ORS - Proposal Approval Form (PAF)
Internal routing For ALL proposals Tracks and
documents clearances and approvals
Identifying information
• Proposal Type• Activity and location• Budget information• Flags UIC contributions • Facilities and
administrative (F&A) distribution
Budget review and approval
Intellectual Property Conflict of interest Signatures
Authorized Representative
Track awards Establish accounts Grants database
Tips for completing PAFTips for completing PAF
Page 1: Project Contact should be familiar with proposal Electronic submission vs. hard copy UIN required for PI and all Co-Investigators if
applicable Identification of original source of funds (federal
flow through) Program name and number Sponsor Information needs to be complete
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Tips for completing the PAFTips for completing the PAF
Page 2: Project information checklist needs to be completed.
One selection per category (ie proposal, award, activity, campus)
Identification of budget period Budget totals should match proposal budget Identify any related cost share Distribution of College and Dept F & A Compliance Checklist – Check all that apply:
• Human Subjects – If applicable, provide Institutional Review Board (IRB) number or identify if “pending”
• Animals – If applicable, requires ACC protocol number • Recombinant DNA – If applicable, require IBC number• Hospital/Clinics – If applicable, require hospital
administration approval. Allow 5 working days
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Tips for completing the PAFTips for completing the PAF
Page 3: Export Controls Approval/Certifications:
• Conflict of Interest Certification – PI including any Co-Investigator(s) must mark “yes” or “no” and sign
• Proposal Approvals:• PI and all co-investigators• Department Head• School/College Dean
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What are Export Controls?
Export Controls Exports are materials, technology, software
and information sent from U.S. to foreign destination
Can be verbal, in writing, by access to items, or even by providing information to a foreign person in U.S.
Federal approval must be obtained prior to the export of any covered equipment, materials, technology, software or information.
Exemptions include: Fundamental research ordinarily published, shared Public domain information Commonly taught scientific principles Disclosure of unclassified technical data in the U.S.
by U.S. institutions of higher learning to foreign FTEs
Documents needed to process Documents needed to process proposalproposal
Minimum documents required to process a proposal: Completed PAF Abstract/Scope of Work Budget
NIH Required Documents: Face Page Abstract/Key Personnel Page Budget Justification Checklist Detailed Budget Modular Budget Page (if applicable)
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Proposal Approval ProcessProposal Approval Process
1. Upon receipt, assigned a PAF/ Institution number
2. Proposal assigned to a research coordinator to review accuracy of information and compliance
3. Reviewer endorses documentation on behalf of the authorized institutional official, Dr. Mitra Dutta
4. ORS staff sends electronic notification to department when proposal is ready to be picked up
5. General turn-around time is 1-2 business days after ORS receives complete proposal package
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Timelines for PAF Approval
Paper Submissions: PAF materials to ORS within 3 days of sponsor deadline.
Electronic Submissions: PAF materials to ORS within 7 days of sponsor deadline
Things that can delay timely approvals Incorrect budget calculations Missing institutional signatures Lack of institutional approvals
Reminders for your budgetReminders for your budget
Always consult program guidelines as they explain:
What can and cannot be budgeting for If there is a cap on the amount that can be
requested What indirect cost rate is allowed
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Distinguishing between Distinguishing between Gifts, Grants and ContractsGifts, Grants and Contracts
Gift – Unrestricted Funds
Grant – Pledge of support for a specific project area, very little if any sponsor involvement and includes specific reporting requirements
Contract – A legal binding agreement whereby a specific project outcome is expected, usually involves some level of sponsor involvement
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Award is here . . . . Now what?Award is here . . . . Now what?
Award notices sent to ORS from sponsor ORS highlights special terms and conditions
and compares award to request If award differs from request a revised
budget is requested from department Revised budget reviewed and approved by
ORS and forwarded to Grants and Contracts for account set-up
Award and supporting documents sent to PI, business manager and Grants and Contracts
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What can slow down your What can slow down your award?award? No proposal/PAF form ever submitted to ORS
Awarded budget does not match the proposed budget and you don’t revise budget in a timely fashion
The award notice is errantly sent to PI, Department, or Grants and Contracts and not forwarded to ORS
Lack of institutional signatures
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ORS on the WebORS on the Web
Research Development Search engines to find funding opportunities Limited competitions Intramural financial resources www.research.uic.edu/funding
Proposal submission Standard Information for applications Facilities and Administrative rates (“F & A”) Sample budgets www.research.uic.edu/funding
Contact Us: Office of Research Contact Us: Office of Research ServicesServices
Luis R. Vargas, Executive Director, [email protected], 312-996-6150
Amneh Kiswani, Associate Director, [email protected], 312-996-9406
Tony Halford, Program Coordinator, [email protected], 312-996-7036
ORS Front Desk: 312-996-2862
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Questions?
Office for the Protection of Research Subjects (OPRS)
Goals of the UIC Human Subjects Protection Program
To ensure a comprehensive Human Subjects Protections Program (HSPP):
• Utilizing the highest ethical and professional standards
• Affording the highest possible protections for our human subjects ■Minimize risk to subjects; and■Maximize benefits of the research
What is research?
Differing perspectives
Research
a systematic investigation including research development, testing, and evaluation; designed to develop or contribute to generalizable knowledge.
45CFR46.102(d)
Human Subjects
living individual(s) about whom an investigator (whether professional or student) conducting research obtains:
(1) data through intervention or interaction with the individual, or
(2) identifiable private information.
45CFR46.102(f)
Note -
Only activities that involve both:Research; andHuman Subjects
are subject to the UIC Human Subjects Protection Program
So, you will be conducting Human Subjects Research at UIC. Now what?....
Tip #1: Utilize Resources
Contact OPRS: 312-996-1711 or [email protected]
On-line Help: “Getting Started - A Guide for
Investigators and Research Staff”• Link:
www.research.uic.edu/protocolreview/irb/getting_started.shtml
Getting Started – Includes information about:
“Human Subjects Research”“Key Research Personnel”Investigator Training requirementsThe role of the UIC IRB The types of protocol review, including:
Determinations Exemptions Expedited IRB Review Convened (full) IRB review Others: Amendments, CR, Prompt Reports
Decision Trees (e.g. medical record review research)Research at the Jesse Brown VAMCTips for obtaining informed consentHelpful links
Tip #2: Complete Investigator Training ASAP
Initial Investigator Training (Investigator 101)• On-Line via CITI; or• In-Person
HIPAA Research Training (if research involves PHI)• On-Line via LMS; or• In-Person
Note: UIC cannot accept applications unless PI training has been completed and is current.
Link to Education and Training: www.research.uic.edu/protocolreview/irb/education/index.shtml
Tip #3: Sign-up for the OPRS NewsletterSent electronically every other month
• News for Investigators• Tips for Investigators• QIP Corner• Continuing Education Opportunities• UIC Data Security Policy Information
Archived on OPRS Web-site
Sign up: Contact OPRS Assistant to the Director, Nora Cortes: [email protected]
Contact Us
West Side Office: Room 203B, AOB,• 1737 West Polk Street • Phone 312-996-1711• Email: [email protected]
East Side Satellite Office (M,W 2 – 4 pm)• Room 3108A, BSB• Phone 312-996-7742
URL: www.research.uic.edu/protocolreview/irb
Questions?
Office ofAnimal Care and Institutional Biosafety (OACIB)
Office for Animal Care and Institutional Biosafety
Oversight for all use of all live vertebrate animals in Research Testing Teaching
Animal Care Animal Care Committee Committee
(ACC)(ACC)
Animal Care Animal Care Committee (ACC)Committee (ACC)
OLAW/NIHUSDAAAALACSPONSORGUIDEILARDEAIDPR
OACIB provides administrative support for the review and approval of research protocols (experiments) involving animals, recombinant DNA and infectious agents, and ensures compliance with regulations related to the use of controlled substances in animal and laboratory research. The goal of the office is to facilitate the protocol writing/review process and navigation of the regulatory issues to ensure compliance with the regulations for the protection and welfare of animals, protection and safety of investigators, and the University.
Office for Animal Care and Institutional Biosafety
Oversight of all research involvingrDNAInfectious agents/toxinsSelect Agents (EHSO)
Institutional Institutional Biosafety Biosafety
Committee (IBC)Committee (IBC)
Institutional Biosafety Institutional Biosafety Committee (IBC)Committee (IBC)
OBA
CDC
USDA
OACIB provides administrative support for the review and approval of research protocols (experiments) involving animals, recombinant DNA and infectious agents, and ensures compliance with regulations related to the use of controlled substances in animal and laboratory research. The goal of the office is to facilitate the protocol writing/review process and navigation of the regulatory issues to ensure compliance with the regulations for the protection and welfare of animals, protection and safety of investigators, and the University.
Office for Animal Care and Institutional Biosafety
Who needs a Who needs a protocol?protocol?
Protocols are needed if research involves the use of animals, rDNA, &/or infectious agents and is conducted at UIC, or is conducted by UIC students &/or paid personnel, or is conducted using funds administered by UIC including subcontracts and consortiums.
Responsibilities?Responsibilities?
Protocol approval is required prior to Prior to initiation of any use of animals JIT or acceptance/transfer of a funding award proposing the use of animals simultaneous with the initiation of some rDNA work (e.g. in vitro BSL 1) prior to the initiation of some rDNA work (e.g., all viral vectors, use in animals, use in humans) prior to the initiation of all infectious agent work
Office for Animal Care and Institutional Biosafety
Who needs to Who needs to be on the be on the protocol?protocol?
Personnel need to be listed on a protocol if they are: PI PI of a grant supported by the protocol (must be Co-PI on the protocol. PPG, Form G protocol exceptions) Working in any capacity with live animals or with rDNA/IA Research Coordinators who need to receive copies of correspondence from OACIB related to protocol issues.
ResponsibilitiesResponsibilities??
Know what is approved on the protocol-
species/strains specific procedures specific experiments specific endpoints or monitoring no migration
Have expertise or get appropriate training in techniques/procedures to be performed Get approval for changes prior to initiation
Facilitating excellence in research at UIC
Office for Animal Care and Institutional Biosafety
ACC Forms and ACC Forms and TrainingTraining
ProtocolsForm A*Form BForm CForm GAppendix 1a, 1b, 1cAppendix 2Appendix 3*
ModificationsForm D
TrainingACCProject Specific
Process and Process and TimingTiming
Protocols ~ 6 weeksNo expedited ReviewVetSubcommitteeFull Committee
ModificationsAdministrative
- 3-7 daysDesignated Reviews
- 1-6 weeksFull
- 1-6 weeks
Facilitating excellence in research at UIC
Office for Animal Care and Institutional Biosafety
IBC Forms and IBC Forms and TrainingTraining
ProtocolsForm AForm BForm DAppendix 1BSL1 ManualBSL2 Manual
ModificationsForm C
TrainingBBPDOT/IATASelect Agents
Process and Process and TimingTiming
Protocols2 weeksPrereviewExpeditedFull
ModificationsExpedited
- 3-7 daysFull
-1-4 weeks
Facilitating excellence in research at UIC
Office for Animal Care and Institutional Biosafety
Post-ApprovalPost-Approval
ACCACC
Animal FacilityOrientationOccupational HealthCard Access
Account CreationAnimal Orders
ApprovedUnapproved
DEA Licenses
ModificationsForm D
Annual RenewalTriennial Review
Post-ApprovalPost-Approval
IBCIBC
Lab InspectionsBSC Certifications
ModificationsForm C
Annual TrainingTriennial Review
Office of Animal Care and Institutional Biosafety
Web Sites
ACC: www.research.uic.edu/protocolreview/acc/index.shtml
IBC: www.research.uic.edu/protocolreview/ibc/index.shtml
Controlled Substances: www.research.uic.edu/protocolreview/cs/index.shtml Meeting Dates FormsEducation/Training Policies/GuidanceFAQs Online Resources
Contact Information
Facilitating excellence in research at UIC
Office of the Vice Chancellor for Research
Animals
rDN
AInfectious agents
Human
s
Chemicals
Toxins
X-rays
Lasers
Controlled Substances
Radioactivity
SelectAgents
Office of Animal Care and Institutional Biosafety
Regulations Pertaining to Research
AnimalsrDNAInfectious AgentsToxins- Biological Controlled SubstancesSelect AgentsHumansChemicalsRadionucleotidesX-rays/IrradiatorsLasersEmbryonic Stem CellsConflict of Time/CommitmentConflict of InterestImport/ExportMaterial Transfer
Office of Animal Care and Institutional Biosafety
Mary Bowman, PhD, DirectorRoom 206 AOB1737 W. PolkPhone: 312.996.1972Email: [email protected]
Contact Us
Questions?
Conflictof Interest (COI)
Conflict of Interest
The Changing Environment
• Federal regulations
• University Policy
Main Points
• COI Defined
• COI and RNUA
• Reporting and Managing Conflicts
• COI Resources
Conflict of Interest
“A ‘conflict of interest’ arises when an academic staff member is in a position to influence either directly or indirectly University business, research, or other decisions in ways that could lead to gain for the academic staff member, the staff member's family, or others to the detriment of the University's integrity and its missions of teaching, research, and public service.”
“Family" is defined as one's spouse or domestic partner, parents, siblings and children. Conflicts reside in a situation, and do not imply improper behavior.
What is financial conflict of interest?
Conflict of Interest
“All paid academic staff members, whether part time or full time, of the University of Illinois. The academic staff includes the faculty ranks of professor, associate professor, assistant professor (and all of the foregoing whose appointments contain such terms as 'research,' 'adjunct,' 'visiting,' or 'clinical'), instructor, and lecturer, as well as academic professionals and postdoctoral associates.”
• Any paid, non-0% appointment. • Students, including medical residents, are not considered academic staff.
Who is covered by the policy?
Conflict of Interest
Annual Reporting of Non University Activities is the implementation of conflict of interest policies and regulations for both conflict of interest and conflict of commitment.
• All academic staff complete the form, even if there are no outside activities to disclose. • 24/7 during the contract period.
Resolution always begins with RNUA disclosure and sometimes involves a combination of conflict management techniques.
Report of Non-University Activities (RNUA)
Conflict of Interest
These regulations require HHS, PHS, and NIH Investigators and senior/key research personnel to: •Disclose significant financial interests (SFIs) at the time of proposal submission •Disclose sponsored or reimbursed travel at the time of proposal submission•Update disclosures within 30 days of any newly acquired or discovered SFIs•Manage Financial Conflicts of Interest (FCOI) prior to execution of an award or continuing review •Complete mandatory University training on Financial Conflicts of Interest (FCOI)
PHS FCOI Regulations (2011)
Conflict of Interest
Annual Disclosure •Annual Report of Non-University Activities (RNUA)
RNUA-Management Plan (RNUA-MP) for situations that require more management (e.g., faculty involved in start-up companies).
•HHS/PHS/NIH – Significant Financial Interest – Disclosure and Management Plan: Part I
SFI-DMP: Part II when University designated officials determine SFI represent a FCOI
Transactional Disclosure • PAF – COI Certification• IRB protocols applications – COI section
How to report and manage COI
Conflict of Interest
Unit Executive Officer Holds primary responsibility for working with the academic staff members to manage conflicts.
UIC Conflict Review Committee (CRC) Advisory to the Vice Chancellor for Research. Reviews RNUA-management plans and other management plans as needed
COI-Human Subject Research (COI-HSR) Committee Expedited review of COI disclosures on IRB protocols Makes recommendations to the IRB.
How to report and manage COI
Conflict of Interest
Online
All RNUA materials (forms, instructions, more) Managing conflicts (templates, instructions, committee info) Policy, rules, regulations FAQs
In Person
Phone, email help Strategy, guidance, and oversight meetings Educational presentations
COI Resources
Contact US
Jacquelyn Jancius, COI DirectorPhone: 312-996-4070Email: [email protected]
Scott MacEwenCOI CoordinatorPhone: 312-996-3642Email: [email protected]
www.research.uic.edu/conflict
Questions?
ResearchIntegrity
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• 1970sWilliam Summerlin, Memorial Sloan-Kettering Cancer Center
the painted mice
• 1980Elias Alsabati, six different US research intitutions
>80 plagiarized and fraudulent papersMarc Straus, Boston University
falsified patient eligibility for clinical trialsVijay Soman, Yale Medical School
falsified and fabricated data plagiarized a rival’s paper
John Long, Massachusetts General Hospital falsely represented monkey cells as human
Research misconduct at top-tier research institutions raise public concerns about policing scientific integrity
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Fraud in Biomedical Research
March 1981 Congressional Oversight HearingsAl Gore, Jr. , Chair, Investigations and Oversight Committee of the House Science and Technology Committee
Philip Handler, President of NAS:
“The system succeeds in policing itself”
Donald Frederickson, Director of NIH:
[no congressional mandate for regulation of research]…is necessary, for the natural sciences contain ultimate correctives for any debasement of the knowledge derived from research.”
Integrity in Research and Publication• Goal
promote compliance with the highest scholarly standards
• Purposeprovide impartial fact-finding and fair adjudication of
allegations of research misconduct• Policy
UI Policy and Procedures on Integrity in Research and Publication
http://www.vpaa.uillinois.edu/policies/Integrity-Policy.pdf
UI Policy incorporates federal regulations and PHS guidelines
42 CFR 93 (rev. 2005) http://www.gpo.gov/fdsys/pkg/CFR-2006-title42-
vol1/pdf/CFR-2006- title42-vol1-part93.pdf
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1985 Health Research Extension Act (Section 493)
HHS regulation requires that institutions establish:• process to review allegations research misconduct • report investigation of alleged research misconduct to the federal government
Integrity in Research and Publication
Research Misconduct includes fabrication, falsification, or plagiarism in proposing, performing, or reviewing research, or in reporting research results.
• Fabrication: making up data or results and recording or reporting them
• Falsification: manipulating research materials, equipment, or processes, or changing or omitting data or results such that the research is not accurately represented in the research record.
• Plagiarism: appropriation of another person’s ideas, processes, results or words without giving appropriate credit
• Does not include honest error or difference of opinion
Integrity in Research and Publication
Research Misconduct also includes, but is not limited to, the following:
• intentionally misleading or deliberately false reporting of credentials and related information
• abuse of confidentiality with respect to unpublished materials
• misappropriation of research materials
• evasion, or intentional failure to comply after notice with research regulations, such as those governing conflict of interest/commitment, human subjects, laboratory animals, new drugs, radioactive materials, genetically altered organisms, and safety
• any other conduct that constitutes a serious deviation from accepted ethical guidelines and professional standards in scholarship and research
Integrity in Research and Publication
• Any member of the University community who has information related to potential Research Misconduct has a duty to report such information to the Respondent’s Unit Executive Officer, or to the RIO
• Members of the University community shall cooperate with the RIO and other institutional officials in the review of allegations and the conduct of inquiries and Investigations.
• Institutional members, including Respondents, have an obligation to provide evidence relevant to Research Misconduct allegations to the RIO or other institutional officials.
Contact US
Mark D. Grabiner, PhDAssociate Vice Chancellor for Research and Research Integrity Officer Phone: 312-996-2757Email: [email protected] Website: www.vpaa.uillinois.edu/Policies/integrityresearch/index.cfm
Questions?
Research Resources Center (RRC)
• Assures accessibility of shared resources to all researchers in the UIC community
• Serves >1,000 investigators, $160M 013 grants
• $8 M operating budget, 35-40% subsidy• 60 staff; full-time PhD lab directors in each
facility• Faculty Academic Directors• East and West Campus central facilities
Recent Advancements
• $12M new capital equipment since 2010• New Imaging Center: 2 of Latest Zeiss Confocals, Prairie
Multiphoton Scope, Vectra tissue imaging• JEOL ARM-200CF Aberration-corrected STEM: very high resolution
EM• Small animal imaging: 9.4T MRI, IVIS live animal imager, VEVO
Echo• 4 new Mass Specs: Orbitrap Velos Pro, Agilent triple quad, AB
Sciex 5500, 6500• New Genomics Center: Sequenome; Gene Titan HT Genotyping;
Ion Torrent, Proton Next-Gen; digital PCR• Drug Discovery Center: High-throughput Screening
• NMR: 600, 750, 800, 900 Mhz• Small molecule and macromolecular XRD, X-ray Crystallography • Biacore molecular interaction analysis• Analytical Ultracentrifugation, Calorimetry, Fluorescence• Production and purification of proteins
Center for Structural BiologyDirector: Yoshitaki Ishii, Technical Director: Ben Ramirez
Confocal Microscopy Facility
The Confocal Microscopy Facility will work to accommodate any biological research that requires light, deconvolution, confocal, TIRF, and/or widefield fluorescent microscopy.State-of-the-art imaging technology for fixed and live cells and tissues.Imaging techniques: live-cell, confocal, FRET, FRAP, FLIP, TIRF, PhotoActivation, and real time recording of micro- injection.
Instruments:Confocals: Two new Zeiss LSM710, Zeiss 510Meta
Olympus VivaView live-cell long-term imaging system
Prairie Systems Multiphoton microscope
Zeiss TIRF System: Newly upgraded; total internal reflection fluorescence; high-resolution imaging of signals near biological membranes.
AIS2 automatic microinjection system: high injection rates of 1,500 cells per hour.
Peter Toth, Technical Director, Ke Ma, associate Director
Contact:[email protected]
Newly rennovated space:2500 sq ft, microscopy suites
ELECTRON MICROSCOPY SERVICE
Scanning (SEM), transmission (TEM) and scanning transmission (STEM) electron microscopes as well as Raman spectroscopy and surface analysis (XPS)
Two laboratories: E5, Medical Sciences Building - SEM and Life Science TEMRoom 116, SES - Materials Science TEM/STEM, Microprobe, Surface Analysis & Raman
NEW: JEOL JEM-ARM200CF TEM/STEMwith cold field emission sourceCEOS probe aberration corrector
Director: Robert Klie, Technical Director: Alan Nichols
The Flow Cytometry Service is a full service facility with seven flow cytometers. We provide flow cytometry services for analysis and sorting of cells, as well as training and expert consultation for project/experiment planning.•High speed sorters: Beckman MoFlo; new cell sorter soming soon•Bio-Rad Bio-Plex for multiplex quantitative assays•5 flow cytometry analyzers: new BD Fortessa, new Beckman Gallios
Types of analysis
Immunophenotyping, DNA content, cell cycle analysis, DNA ploidy analysis, apoptosis, phagocytosis, intracellular pH, calcium levels, oxidative burst, intracellular antigen measurement, total protein, cytokine detection, intracellular signaling and isolation of rare cell populations including human and animal stem cells.
Flow Cytometry Service FCSBalaji Ganesh, Director
Staff
Jewell Graves, Lab Manager
Juan Chen, Research Specialist
Lakshmy Ganesh, Research Specialist
Appointments can be made for instrument instruction, sorts, and use of our flow cytometers on the FCS Online scheduler proteomics.rrc.uic.edu/flow.
Raw data and analysis files will be sent on line to with the "MY DATA" server.
Beckman Coulter Cyan ADP
Bio-Rad Bio-Plex
Beckman Coulter MoFlo
Becton Dickinson Fortessa
Transgenic Production Service
• DNA Microinjection/Transgenics• ES Cell Microinjection/Chimeric mice• ES cell expansion• Gene Targeting in ES cells• Embryo & Sperm Cryopreservation• Recovery of Pups from Cryopreserved
Embryos• Recovery of Pups from Cryopreserved
Sperm• In Vitro Fertilization (IVF)
The Transgenic Production Service provides a resource for investigators seeking to generate transgenic and gene-targeted mice for their research activities.
Animal Imaging and phenotyping
The Xenogen IVIS® Spectrum in vivo imaging system uses optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals.
Preclinical MRI Facility: 2,000 sq. ft. animal imaging laboratory equipped with a new Agilent 9.4 Tesla MRI system that is able to image small animals and samples up to 7.5 cm in diameter.
CCVR:Surgical services: Trans aortic constriction; Coronary artery ligation; Osmotic plant implantationImaging: VisualSonics Vevo770 ultrasound
Services Available
Tissues embedded in paraffin and processed for sectioning
Training and consultation available for by phone, e-mail or in person.
Antibody testing for determination of optimal conditions
Immunofluorescence of cultured cells
Fluorescent or colorimetric detection of antigens by immunohistochemistry
TUNEL staining in fixed sections for apoptosis studies
Routine Hematoxylin and Eosin staining of tissue sections
Specialized stains, such as the Gomori Trichrome Stain
Sample preparation/OCT freezing for cryosectioning
Tissue preparation for electron microscopy
Imaging: Nikon E400 fluorescent microscope equipped with a SPOT color camera.
ContactAndy Hall www.rrc.uic.edu/rhc
Microm Vacutome Cryostat service, or can be reserved to do your own sectioning
Microm Vacutome Cryostat service, or can be reserved to do your own sectioning
3 microtomes:Do your own or we do the work for you
Routine histology staining or immuno-histochemical staining
Nikon Eclipse E400 Epi-fluorescence microscope,
Immunohistochemical localization of cFOS inthe baboon endometrium visualized with DAB. Ha
Dual staining of Connexin 26 (green) and cytokeratin (red) in the baboon endometrium.
Research Histology Core (RHC)Director: Andy Hall COMRB 1120
Biorepository• Institutional resource for processing and
storing human tissue samples for research purposes.
• New LN and -85 freezers house blood, serum, plasma, urine, nucleic acids, & PBMC specimens
• IRB-approved opt-in protocol
• Specimens are banked in compliance with IRB-approved protocols and HIPAA standards
• Investigator protocols; 10 current, others in IRB
SERVICES INCLUDE:
•Separation of whole blood derivatives serum, plasma, PBMCs, urine, and saliva.
•Nucleic Acid isolation using iPrep™
•Opt-in or PI protocol; all samples bar coded and annotated
•Specimen management database moving from caTissue to OnCore
•Secure storage of samples/data
STAFFING:
•Director•2 Pathologists
• 2 Path Assistants• 2 Techs
BioInformatics Service
For analysis of big datasets from Genomics/Sequencing Resources
Integrated with Genomics, computing IT and Biorepository
Staff:• 4 Bioinformaticians specializing in analysis of
genome sequencing, gene expression, genotyping, pathways
Computing resources• Mixed cluster (GPU/CPU)• HPC coming soon• UIUC computing resources
New 3,000 sq ft facility in CME
Director: Ives Lussier, Technical Director: Neil Bahroos
Center for Genomic ResearchStaff: Co-Directors, 8 full time technicians, 4 students
Budget: $1.6M/yr
Services: • Next-Generation Sequencing• CE Sequencing• Quantitative analyses- QPCR
and Digital PCR• Automated Nucleic Acid purification
and QC• Gene expression analysis • High Throughput Genotyping, CNV• Cytogenomics
Select Capabilities Whole genome sequencing (De novo and re-sequencing)
• Ion Torrent Proton and PGM (UIC)
• Illumina HiSeq2000, Roche 454 (UIUC)
Target capture (custom/cancer panel/exome)
• Agilent SureSelect / Haloplex (hybridization capture)
• Ion Torrent Ampliseq / Qiagen Generead (PCR-based)
ChIP-seq: Sample prep with Covaris acoustic shearing
Microbiome Analysis: Ribosomal RNA gene sequencing
Genotyping: Affymetrix Gene Titan: 96 sample 2.2M microarray
• Sequenome: 768 sample throughput
Transcriptome sequencing
Differential gene expression
• Detection of novel transcripts
• Illumina HiSeq / Ion Torrent Proton
MicroRNA: Ion Torrent Proton; TILDA microfluidic arrays
Quantitative mRNA expression analysis
• Affymetrix Quantigene Plex: 3 to 80-plex; 96-sample throughput
• Affymetrix micro array
Cytoscan Affymetrix cytogenetic profiling
Mass Spectrometry, Metabolomics and Proteomics
Waters Synapt QTOF MS; ion mobility
Richard van Breemen, Academic Director; Alex Schilling, Technical Director Primary Staff: Rod Davis, Jerry White
2 Agilent 6410 Triple quadsChipCube system
Thermo LTQ-FT LC/MS/MS
Thermo Velos Orbitrap Pro funded by NCRRFitted with Chip Cube
•Mascot & SEQUEST search engines •Scaffold report generation: easier to interpret, interact with your results•Self-serve informatics workstations BioWorks and file conversion tools•X!Tandem searches•Specialized tools, customized databases
Services Provided• Accurate Mass Measurement• LC-MS and LC-MS-MS• MALDI MS and MS-MS• Quantitative Analysis• Structure Elucidation• Protein Identification• Metabolite Identification
Other Instrumentation• New Triple Quad ABSciex 5500• Shimazdu IT-TOF MSn
• JOEL GC-MATE MS• Thermo TSQ Quantum QQQ MS-MS• Agilent HPLC Chip Cube Interface• Agilent 1946C MSD MS• Thermo LCQDeca MSn
• Waters SCF-MS• Shimazdu LCMS 8030 QQQ MS-MS• Agilent Off-gel • Thermo LTQ MSn
Informatics Tools
The Scientific Instrument Shop (SIS) is staffed by Instrument Makers, Senior Laboratory Mechanics, and Biomedical Electronics Engineers who construct and repair all types of mechanical and electronic/electrical equipment for the research, teaching and clinical requirements of faculty, staff and students.
Staff
Electronics Shop: complete electronic services including the maintenance and repair of electronic devices. We also design and fabricate customized electronic equipment that may not be commercially available.
Eric Schmidt [email protected]
John Grega, Biomedical Engineering Technician
Matthew Schuck, Instrument Maker
Machine ShopSkilled Instrument Makers will assist designing and/or fabricating items utilizing the latest CAD/CAM software; full 3-axis and 3-D contour milling.
Materials: stainless and alloy steels, titanium, lead, platinum, brass, aluminum, copper, sheet metalplastics: acrylic, acetate, acetal, polypropylene, polystyrene, nylon, PVC, teflon, polycarbonates, polyethylene, engineered plastics wood, wood composites and machinable ceramics.
Capabilities:Turning in lathe up to 15" Manual milling 2 axes and 3 axes CNC milling Surface grinding Precision thread cutting Sheetmetal bending Forming, shearing and fabrication Hole punching Woodworking — sawing and fabrication
Scientific Instrumentation ShopDirector: Eric Schmidt
ServicesTig welding of most metals Brazing silver solder Sandblasting Thermocouples Repair and rebuilding Fabrication of parts- most plasticsmachining, plastic welding/gluing,bending and polishing
Contact US
William HendricksonDirector, RRCPhone: 312-996-5600Email: [email protected] Website: http://www.rrc.uic.edu/
Questions?
Biologic Resources Laboratory(BRL)
Biologic Resources Laboratory
• UIC’s Centralized Animal Research Facility• Oversight of the institution’s animal care and
use program• Support research• Daily care and husbandry of all laboratory animals• Procurement of all animals• Ensure animal welfare • Ensure compliance with regulatory agencies• Ensure the health status of animal colonies• Train students, staff, and faculty
Biologic Resources Laboratory
•UIC’s Animal Care and Use Program has been continually accredited by AAALAC International since 1970.
•Oldest and most successful training programs for laboratory animal veterinarians
•Active professional staff • Professional organizations• Teaching• Research• Service
Biologic Resources LaboratoryProgram Scope
•137,000 sq. ft.•10 satellite facilities•House over 20 species•Ave. daily census 32,000 animals
• Mice 30,000• Primates 175
Biologic Resources LaboratoryProgram Scope• Staff - 64
• 11 vets – 5 board certified, 6 post-docs• 8 veterinary technicians• 37 husbandry staff• 6 administrative staff • 1enrichment specialist• 1 machinist
Biologic Resources LaboratoryAnimal Based Research Scope
•275 investigators•700 active research protocols•Types of studies
• Cancer• Immunotherapy• Radiobiology• Infectious disease• TransplantationHeart Disease• Diabetes• Neurologic disease• Retinal diseases
Biologic Resources Laboratory Services
• To gain access to any of the facilities• Listed on an approved ACC protocol• Orientation with one of the veterinarians• Visit employee health and enroll in the
occupational health and safety program
Biologic Resources Laboratory Services• Centralized surgery• Diagnostic laboratory• Radiology section• Antibody production service• Cross foster rederivation program • Support Good Laboratory Practice Studies• Support bio and chemical hazard work• Irradiator• Metabolic cages• Rodent quarantine• Resource
Biologic Resources Laboratory
• Main phone number 312-996-7040• Website http://www.brl.uic.edu/
• Key contacts• Fee schedule
Animal per diem rateDiagnostic lab servicesSurgery services
• Jeffrey Fortman, Director Biologic Resources Lab
• 312-996-1220• [email protected]
Questions?