Office of the Vice Chancellor for Research New Faculty Orientation

Office of the Vice Chancellor for Research

New Faculty Orientation

Office ofResearchServices (ORS)

Office of Research Services &Office of Research Services &Research Development Services Research Development Services

Helping you navigate your research portfolio

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Total DollarsAwarded – FY 2008- FY 2012

0

50

100

150

200

250

300

350

400

450

2008 2009 2010 2011 2012

Mil

lio

ns

*2010 includes ARRA awards

Office of Research Services (ORS)

•Research Development•Proposal and award processing

• Pre-award• Post award (non financial)

•Budget review• Fit with scope

• Statutory, fiscal compliance

•All grant submissions• Sponsors: Government,

industry, foundations, etc.• Modes: Grants.gov, Proposal

Central, paper, etc.

•All grant and contract approvals

• All negotiations • Other agreements

• Material transfer• Professional service

agreements

•Standard information•Sponsor policy updates• Procedural guidance

Research Development Tools, Resources, and Processes

•Searchable Databases•Limited Competitions•Internal Funding

Finding FundingFinding Funding

Resources to find grants matching your research interests

Examples:• Pivot-COS• Grants.gov• Grants Resource Center (GRC)• IRIS• FedBizOpps

Pivot-COSPivot-COS

Pivot - Community of Science• All disciplines, all sponsor types,

international• Save multiple, customized funding alerts• Track opportunities• Matching function• Find collaborators

http://pivot.cos.com/

GRCGRC

Grants Resource Center• Maintains own database and publications that

cover all relevant federal grants and many private grants

• Maintains a library of funded proposals• Connect faculty members with program officers

www.aascu.org/grc (login: uic, pswd: awards)

Limited Competitions

• Sponsor makes university prescreen and select proposal

• Peer reviewed – typically evaluated for: • Program Compliance• Scientific Merit• Management

• Education/Training • MUST have prior authorization to

submit

Limited Competition Process

1. Announcement2. Letter of Intent3. White Papers (if necessary) 4. Peer Review ( if necessary)5. Authorization6. Feedback (if warranted)

This process typically takes from 2 to 4 weeks

Campus Research Board Campus Research Board Pilot Grants Pilot Grants

Up to $20,000 over 1 year Purpose:

• Foster pilot research• Nurture career trajectories of promising early-stage

investigators• Leverage extramural funding

Eligibility: • FT tenure or non-tenure track faculty • Within 8 years of terminal degree

Fall Deadline: • LOI: late summer, 2013 • Application: Autumn, 2013

http://tigger.uic.edu/depts/ovcr/research/funding/CRB.html#eligibility

Chancellor’s Discovery Fund Chancellor’s Discovery Fund for Multidisciplinary Research for Multidisciplinary Research

Up to $40,000 over 2 years Purpose:

• Foster pilot research using the expertise of varied disciplines• Nurture career trajectories of promising early-stage

investigators• Leverage extramural funding• Encourage new or support early stage collaborations

Eligibility: • FT tenure or non-tenure track faculty • Minimum of two Principal Investigators• At least one PI must be within 8 years of their first faculty

appointment FY 13 Deadline:

• LOI: Fall, 2013 (date TBD)• Application: January, 2013

http://tigger.uic.edu/depts/ovcr/research/funding/discovery/

Bridge Funding ProgramBridge Funding Program

Purpose: short term support to sustain research programs, laboratories, and momentum of productive researchers during funding gaps to give them a chance to regain extramural funding.

Eligibility • Full time (at least .8 FTE) senior faculty. Preference will

be given to Associate Professors • Must have been PI on a grant funded at least three

consecutive years (the same grant or project) by a national agency with a bona fide scientific peer-review process (e.g. NSF, NIH, AHA)

Awards: Vary by Phase• 20K for one year or less. Must be matched 1:1

Next deadline: TBD

http://tigger.uic.edu/depts/ovcr/research/funding/Seed_Funding/ovcr_bridge.html#awards

Ignite Proposal Development Ignite Proposal Development ProgramProgram Purpose: Spark proposal planning or

development process Eligibility

• Lead PI must be tenured or tenure track faculty and have a full-time (at least .8 FTE) UIC appointment

• Project must involve 3 or more colleges. Awards: Vary by Phase

• 20K for a six month term AY 2013 deadlines: TBD

http://tigger.uic.edu/depts/ovcr/research/funding/ignite/

Office of Research Services Office of Research Services (ORS)(ORS) Supports faculty from development to

award stage

Authorized institutional representative • Official institutional endorsements• Authorizes all submissions • Accepts all awards• Negotiates all contracts • Government, for profit industry, not for profit

foundations

Central point of coordination for all sponsored projects for the Chicago, Rockford and Peoria campuses

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Internal Approvals: Cross your T, Dot your I, CYA

ORS - Proposal Approval Form (PAF)

Internal routing For ALL proposals Tracks and

documents clearances and approvals

Identifying information

• Proposal Type• Activity and location• Budget information• Flags UIC contributions • Facilities and

administrative (F&A) distribution

Budget review and approval

Intellectual Property Conflict of interest Signatures

Authorized Representative

Track awards Establish accounts Grants database

Tips for completing PAFTips for completing PAF

Page 1: Project Contact should be familiar with proposal Electronic submission vs. hard copy UIN required for PI and all Co-Investigators if

applicable Identification of original source of funds (federal

flow through) Program name and number Sponsor Information needs to be complete

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Tips for completing the PAFTips for completing the PAF

Page 2: Project information checklist needs to be completed.

One selection per category (ie proposal, award, activity, campus)

Identification of budget period Budget totals should match proposal budget Identify any related cost share Distribution of College and Dept F & A Compliance Checklist – Check all that apply:

• Human Subjects – If applicable, provide Institutional Review Board (IRB) number or identify if “pending”

• Animals – If applicable, requires ACC protocol number • Recombinant DNA – If applicable, require IBC number• Hospital/Clinics – If applicable, require hospital

administration approval. Allow 5 working days

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Tips for completing the PAFTips for completing the PAF

Page 3: Export Controls Approval/Certifications:

• Conflict of Interest Certification – PI including any Co-Investigator(s) must mark “yes” or “no” and sign

• Proposal Approvals:• PI and all co-investigators• Department Head• School/College Dean

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What are Export Controls?

Export Controls Exports are materials, technology, software

and information sent from U.S. to foreign destination

Can be verbal, in writing, by access to items, or even by providing information to a foreign person in U.S.

Federal approval must be obtained prior to the export of any covered equipment, materials, technology, software or information.

Exemptions include: Fundamental research ordinarily published, shared Public domain information Commonly taught scientific principles Disclosure of unclassified technical data in the U.S.

by U.S. institutions of higher learning to foreign FTEs

Documents needed to process Documents needed to process proposalproposal

Minimum documents required to process a proposal: Completed PAF Abstract/Scope of Work Budget

NIH Required Documents: Face Page Abstract/Key Personnel Page Budget Justification Checklist Detailed Budget Modular Budget Page (if applicable)

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Proposal Approval ProcessProposal Approval Process

1. Upon receipt, assigned a PAF/ Institution number

2. Proposal assigned to a research coordinator to review accuracy of information and compliance

3. Reviewer endorses documentation on behalf of the authorized institutional official, Dr. Mitra Dutta

4. ORS staff sends electronic notification to department when proposal is ready to be picked up

5. General turn-around time is 1-2 business days after ORS receives complete proposal package

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Timelines for PAF Approval

Paper Submissions: PAF materials to ORS within 3 days of sponsor deadline.

Electronic Submissions: PAF materials to ORS within 7 days of sponsor deadline

Things that can delay timely approvals Incorrect budget calculations Missing institutional signatures Lack of institutional approvals

Reminders for your budgetReminders for your budget

Always consult program guidelines as they explain:

What can and cannot be budgeting for If there is a cap on the amount that can be

requested What indirect cost rate is allowed

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Distinguishing between Distinguishing between Gifts, Grants and ContractsGifts, Grants and Contracts

Gift – Unrestricted Funds

Grant – Pledge of support for a specific project area, very little if any sponsor involvement and includes specific reporting requirements

Contract – A legal binding agreement whereby a specific project outcome is expected, usually involves some level of sponsor involvement

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Award is here . . . . Now what?Award is here . . . . Now what?

Award notices sent to ORS from sponsor ORS highlights special terms and conditions

and compares award to request If award differs from request a revised

budget is requested from department Revised budget reviewed and approved by

ORS and forwarded to Grants and Contracts for account set-up

Award and supporting documents sent to PI, business manager and Grants and Contracts

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What can slow down your What can slow down your award?award? No proposal/PAF form ever submitted to ORS

Awarded budget does not match the proposed budget and you don’t revise budget in a timely fashion

The award notice is errantly sent to PI, Department, or Grants and Contracts and not forwarded to ORS

Lack of institutional signatures

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ORS on the WebORS on the Web

Research Development Search engines to find funding opportunities Limited competitions Intramural financial resources www.research.uic.edu/funding

Proposal submission Standard Information for applications Facilities and Administrative rates (“F & A”) Sample budgets www.research.uic.edu/funding

Contact Us: Office of Research Contact Us: Office of Research ServicesServices

Luis R. Vargas, Executive Director, [email protected], 312-996-6150

Amneh Kiswani, Associate Director, [email protected], 312-996-9406

Tony Halford, Program Coordinator, [email protected], 312-996-7036

ORS Front Desk: 312-996-2862

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Questions?

Office for the Protection of Research Subjects (OPRS)

Goals of the UIC Human Subjects Protection Program

To ensure a comprehensive Human Subjects Protections Program (HSPP):

• Utilizing the highest ethical and professional standards

• Affording the highest possible protections for our human subjects ■Minimize risk to subjects; and■Maximize benefits of the research

What is research?

Differing perspectives

Research

a systematic investigation including research development, testing, and evaluation; designed to develop or contribute to generalizable knowledge.

45CFR46.102(d)

Human Subjects

living individual(s) about whom an investigator (whether professional or student) conducting research obtains:

(1) data through intervention or interaction with the individual, or

(2) identifiable private information.

45CFR46.102(f)

Note -

Only activities that involve both:Research; andHuman Subjects

are subject to the UIC Human Subjects Protection Program

So, you will be conducting Human Subjects Research at UIC. Now what?....

Tip #1: Utilize Resources

Contact OPRS: 312-996-1711 or [email protected]

On-line Help: “Getting Started - A Guide for

Investigators and Research Staff”• Link:

www.research.uic.edu/protocolreview/irb/getting_started.shtml

Getting Started – Includes information about:

“Human Subjects Research”“Key Research Personnel”Investigator Training requirementsThe role of the UIC IRB The types of protocol review, including:

Determinations Exemptions Expedited IRB Review Convened (full) IRB review Others: Amendments, CR, Prompt Reports

Decision Trees (e.g. medical record review research)Research at the Jesse Brown VAMCTips for obtaining informed consentHelpful links

Tip #2: Complete Investigator Training ASAP

Initial Investigator Training (Investigator 101)• On-Line via CITI; or• In-Person

HIPAA Research Training (if research involves PHI)• On-Line via LMS; or• In-Person

Note: UIC cannot accept applications unless PI training has been completed and is current.

Link to Education and Training: www.research.uic.edu/protocolreview/irb/education/index.shtml

Tip #3: Sign-up for the OPRS NewsletterSent electronically every other month

• News for Investigators• Tips for Investigators• QIP Corner• Continuing Education Opportunities• UIC Data Security Policy Information

Archived on OPRS Web-site

Sign up: Contact OPRS Assistant to the Director, Nora Cortes: [email protected]

Contact Us

West Side Office: Room 203B, AOB,• 1737 West Polk Street • Phone 312-996-1711• Email: [email protected]

East Side Satellite Office (M,W 2 – 4 pm)• Room 3108A, BSB• Phone 312-996-7742

URL: www.research.uic.edu/protocolreview/irb

Questions?

Office ofAnimal Care and Institutional Biosafety (OACIB)

Office for Animal Care and Institutional Biosafety

Oversight for all use of all live vertebrate animals in Research Testing Teaching

Animal Care Animal Care Committee Committee

(ACC)(ACC)

Animal Care Animal Care Committee (ACC)Committee (ACC)

OLAW/NIHUSDAAAALACSPONSORGUIDEILARDEAIDPR

OACIB provides administrative support for the review and approval of research protocols (experiments) involving animals, recombinant DNA and infectious agents, and ensures compliance with regulations related to the use of controlled substances in animal and laboratory research. The goal of the office is to facilitate the protocol writing/review process and navigation of the regulatory issues to ensure compliance with the regulations for the protection and welfare of animals, protection and safety of investigators, and the University.


Oversight of all research involvingrDNAInfectious agents/toxinsSelect Agents (EHSO)

Institutional Institutional Biosafety Biosafety

Committee (IBC)Committee (IBC)

Institutional Biosafety Institutional Biosafety Committee (IBC)Committee (IBC)

OBA

CDC

USDA

OACIB provides administrative support for the review and approval of research protocols (experiments) involving animals, recombinant DNA and infectious agents, and ensures compliance with regulations related to the use of controlled substances in animal and laboratory research. The goal of the office is to facilitate the protocol writing/review process and navigation of the regulatory issues to ensure compliance with the regulations for the protection and welfare of animals, protection and safety of investigators, and the University.


Who needs a Who needs a protocol?protocol?

Protocols are needed if research involves the use of animals, rDNA, &/or infectious agents and is conducted at UIC, or is conducted by UIC students &/or paid personnel, or is conducted using funds administered by UIC including subcontracts and consortiums.

Responsibilities?Responsibilities?

Protocol approval is required prior to Prior to initiation of any use of animals JIT or acceptance/transfer of a funding award proposing the use of animals simultaneous with the initiation of some rDNA work (e.g. in vitro BSL 1) prior to the initiation of some rDNA work (e.g., all viral vectors, use in animals, use in humans) prior to the initiation of all infectious agent work


Who needs to Who needs to be on the be on the protocol?protocol?

Personnel need to be listed on a protocol if they are: PI PI of a grant supported by the protocol (must be Co-PI on the protocol. PPG, Form G protocol exceptions) Working in any capacity with live animals or with rDNA/IA Research Coordinators who need to receive copies of correspondence from OACIB related to protocol issues.

ResponsibilitiesResponsibilities??

Know what is approved on the protocol-

species/strains specific procedures specific experiments specific endpoints or monitoring no migration

Have expertise or get appropriate training in techniques/procedures to be performed Get approval for changes prior to initiation

Facilitating excellence in research at UIC


ACC Forms and ACC Forms and TrainingTraining

ProtocolsForm A*Form BForm CForm GAppendix 1a, 1b, 1cAppendix 2Appendix 3*

ModificationsForm D

TrainingACCProject Specific

Process and Process and TimingTiming

Protocols ~ 6 weeksNo expedited ReviewVetSubcommitteeFull Committee

ModificationsAdministrative

- 3-7 daysDesignated Reviews

- 1-6 weeksFull

- 1-6 weeks



IBC Forms and IBC Forms and TrainingTraining

ProtocolsForm AForm BForm DAppendix 1BSL1 ManualBSL2 Manual

ModificationsForm C

TrainingBBPDOT/IATASelect Agents

Process and Process and TimingTiming

Protocols2 weeksPrereviewExpeditedFull

ModificationsExpedited

- 3-7 daysFull

-1-4 weeks



Post-ApprovalPost-Approval

ACCACC

Animal FacilityOrientationOccupational HealthCard Access

Account CreationAnimal Orders

ApprovedUnapproved

DEA Licenses

ModificationsForm D

Annual RenewalTriennial Review

Post-ApprovalPost-Approval

IBCIBC

Lab InspectionsBSC Certifications

ModificationsForm C

Annual TrainingTriennial Review

Office of Animal Care and Institutional Biosafety

Web Sites

ACC: www.research.uic.edu/protocolreview/acc/index.shtml

IBC: www.research.uic.edu/protocolreview/ibc/index.shtml

Controlled Substances: www.research.uic.edu/protocolreview/cs/index.shtml Meeting Dates FormsEducation/Training Policies/GuidanceFAQs Online Resources

Contact Information


Office of the Vice Chancellor for Research

Animals

rDN

AInfectious agents

Human

s

Chemicals

Toxins

X-rays

Lasers

Controlled Substances

Radioactivity

SelectAgents


Regulations Pertaining to Research

AnimalsrDNAInfectious AgentsToxins- Biological Controlled SubstancesSelect AgentsHumansChemicalsRadionucleotidesX-rays/IrradiatorsLasersEmbryonic Stem CellsConflict of Time/CommitmentConflict of InterestImport/ExportMaterial Transfer


Mary Bowman, PhD, DirectorRoom 206 AOB1737 W. PolkPhone: 312.996.1972Email: [email protected]

Contact Us

Questions?

Conflictof Interest (COI)

Conflict of Interest

The Changing Environment

• Federal regulations

• University Policy

Main Points

• COI Defined

• COI and RNUA

• Reporting and Managing Conflicts

• COI Resources


“A ‘conflict of interest’ arises when an academic staff member is in a position to influence either directly or indirectly University business, research, or other decisions in ways that could lead to gain for the academic staff member, the staff member's family, or others to the detriment of the University's integrity and its missions of teaching, research, and public service.”

“Family" is defined as one's spouse or domestic partner, parents, siblings and children. Conflicts reside in a situation, and do not imply improper behavior.

What is financial conflict of interest?


“All paid academic staff members, whether part time or full time, of the University of Illinois. The academic staff includes the faculty ranks of professor, associate professor, assistant professor (and all of the foregoing whose appointments contain such terms as 'research,' 'adjunct,' 'visiting,' or 'clinical'), instructor, and lecturer, as well as academic professionals and postdoctoral associates.”

• Any paid, non-0% appointment. • Students, including medical residents, are not considered academic staff.

Who is covered by the policy?


Annual Reporting of Non University Activities is the implementation of conflict of interest policies and regulations for both conflict of interest and conflict of commitment.

• All academic staff complete the form, even if there are no outside activities to disclose. • 24/7 during the contract period.

Resolution always begins with RNUA disclosure and sometimes involves a combination of conflict management techniques.

Report of Non-University Activities (RNUA)


These regulations require HHS, PHS, and NIH Investigators and senior/key research personnel to: •Disclose significant financial interests (SFIs) at the time of proposal submission •Disclose sponsored or reimbursed travel at the time of proposal submission•Update disclosures within 30 days of any newly acquired or discovered SFIs•Manage Financial Conflicts of Interest (FCOI) prior to execution of an award or continuing review •Complete mandatory University training on Financial Conflicts of Interest (FCOI)

PHS FCOI Regulations (2011)


Annual Disclosure •Annual Report of Non-University Activities (RNUA)

RNUA-Management Plan (RNUA-MP) for situations that require more management (e.g., faculty involved in start-up companies).

•HHS/PHS/NIH – Significant Financial Interest – Disclosure and Management Plan: Part I

SFI-DMP: Part II when University designated officials determine SFI represent a FCOI

Transactional Disclosure • PAF – COI Certification• IRB protocols applications – COI section

How to report and manage COI


Unit Executive Officer Holds primary responsibility for working with the academic staff members to manage conflicts.

UIC Conflict Review Committee (CRC) Advisory to the Vice Chancellor for Research. Reviews RNUA-management plans and other management plans as needed

COI-Human Subject Research (COI-HSR) Committee Expedited review of COI disclosures on IRB protocols Makes recommendations to the IRB.

How to report and manage COI


Online

All RNUA materials (forms, instructions, more) Managing conflicts (templates, instructions, committee info) Policy, rules, regulations FAQs

In Person

Phone, email help Strategy, guidance, and oversight meetings Educational presentations

COI Resources

Contact US

Jacquelyn Jancius, COI DirectorPhone: 312-996-4070Email: [email protected]

Scott MacEwenCOI CoordinatorPhone: 312-996-3642Email: [email protected]

www.research.uic.edu/conflict

Questions?

ResearchIntegrity

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• 1970sWilliam Summerlin, Memorial Sloan-Kettering Cancer Center

the painted mice

• 1980Elias Alsabati, six different US research intitutions

>80 plagiarized and fraudulent papersMarc Straus, Boston University

falsified patient eligibility for clinical trialsVijay Soman, Yale Medical School

falsified and fabricated data plagiarized a rival’s paper

John Long, Massachusetts General Hospital falsely represented monkey cells as human

Research misconduct at top-tier research institutions raise public concerns about policing scientific integrity

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Fraud in Biomedical Research

March 1981 Congressional Oversight HearingsAl Gore, Jr. , Chair, Investigations and Oversight Committee of the House Science and Technology Committee

Philip Handler, President of NAS:

“The system succeeds in policing itself”

Donald Frederickson, Director of NIH:

[no congressional mandate for regulation of research]…is necessary, for the natural sciences contain ultimate correctives for any debasement of the knowledge derived from research.”

Integrity in Research and Publication• Goal

promote compliance with the highest scholarly standards

• Purposeprovide impartial fact-finding and fair adjudication of

allegations of research misconduct• Policy

UI Policy and Procedures on Integrity in Research and Publication

http://www.vpaa.uillinois.edu/policies/Integrity-Policy.pdf

UI Policy incorporates federal regulations and PHS guidelines

42 CFR 93 (rev. 2005) http://www.gpo.gov/fdsys/pkg/CFR-2006-title42-

vol1/pdf/CFR-2006- title42-vol1-part93.pdf

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1985 Health Research Extension Act (Section 493)

HHS regulation requires that institutions establish:• process to review allegations research misconduct • report investigation of alleged research misconduct to the federal government

Integrity in Research and Publication

Research Misconduct includes fabrication, falsification, or plagiarism in proposing, performing, or reviewing research, or in reporting research results.

• Fabrication: making up data or results and recording or reporting them

• Falsification: manipulating research materials, equipment, or processes, or changing or omitting data or results such that the research is not accurately represented in the research record.

• Plagiarism: appropriation of another person’s ideas, processes, results or words without giving appropriate credit

• Does not include honest error or difference of opinion


Research Misconduct also includes, but is not limited to, the following:

• intentionally misleading or deliberately false reporting of credentials and related information

• abuse of confidentiality with respect to unpublished materials

• misappropriation of research materials

• evasion, or intentional failure to comply after notice with research regulations, such as those governing conflict of interest/commitment, human subjects, laboratory animals, new drugs, radioactive materials, genetically altered organisms, and safety

• any other conduct that constitutes a serious deviation from accepted ethical guidelines and professional standards in scholarship and research


• Any member of the University community who has information related to potential Research Misconduct has a duty to report such information to the Respondent’s Unit Executive Officer, or to the RIO

• Members of the University community shall cooperate with the RIO and other institutional officials in the review of allegations and the conduct of inquiries and Investigations.

• Institutional members, including Respondents, have an obligation to provide evidence relevant to Research Misconduct allegations to the RIO or other institutional officials.

Contact US

Mark D. Grabiner, PhDAssociate Vice Chancellor for Research and Research Integrity Officer Phone: 312-996-2757Email: [email protected] Website: www.vpaa.uillinois.edu/Policies/integrityresearch/index.cfm

Questions?

Research Resources Center (RRC)

• Assures accessibility of shared resources to all researchers in the UIC community

• Serves >1,000 investigators, $160M 013 grants

• $8 M operating budget, 35-40% subsidy• 60 staff; full-time PhD lab directors in each

facility• Faculty Academic Directors• East and West Campus central facilities

Recent Advancements

• $12M new capital equipment since 2010• New Imaging Center: 2 of Latest Zeiss Confocals, Prairie

Multiphoton Scope, Vectra tissue imaging• JEOL ARM-200CF Aberration-corrected STEM: very high resolution

EM• Small animal imaging: 9.4T MRI, IVIS live animal imager, VEVO

Echo• 4 new Mass Specs: Orbitrap Velos Pro, Agilent triple quad, AB

Sciex 5500, 6500• New Genomics Center: Sequenome; Gene Titan HT Genotyping;

Ion Torrent, Proton Next-Gen; digital PCR• Drug Discovery Center: High-throughput Screening

• NMR: 600, 750, 800, 900 Mhz• Small molecule and macromolecular XRD, X-ray Crystallography • Biacore molecular interaction analysis• Analytical Ultracentrifugation, Calorimetry, Fluorescence• Production and purification of proteins

Center for Structural BiologyDirector: Yoshitaki Ishii, Technical Director: Ben Ramirez

Confocal Microscopy Facility

The Confocal Microscopy Facility will work to accommodate any biological research that requires light, deconvolution, confocal, TIRF, and/or widefield fluorescent microscopy.State-of-the-art imaging technology for fixed and live cells and tissues.Imaging techniques: live-cell, confocal, FRET, FRAP, FLIP, TIRF, PhotoActivation, and real time recording of microinjection.

Instruments:Confocals: Two new Zeiss LSM710, Zeiss 510Meta

Olympus VivaView live-cell long-term imaging system

Prairie Systems Multiphoton microscope

Zeiss TIRF System: Newly upgraded; total internal reflection fluorescence; high-resolution imaging of signals near biological membranes.

AIS2 automatic microinjection system: high injection rates of 1,500 cells per hour.

Peter Toth, Technical Director, Ke Ma, associate Director

Contact:[email protected]

Newly rennovated space:2500 sq ft, microscopy suites

ELECTRON MICROSCOPY SERVICE

Scanning (SEM), transmission (TEM) and scanning transmission (STEM) electron microscopes as well as Raman spectroscopy and surface analysis (XPS)

Two laboratories: E5, Medical Sciences Building - SEM and Life Science TEMRoom 116, SES - Materials Science TEM/STEM, Microprobe, Surface Analysis & Raman

NEW: JEOL JEM-ARM200CF TEM/STEMwith cold field emission sourceCEOS probe aberration corrector

Director: Robert Klie, Technical Director: Alan Nichols

The Flow Cytometry Service is a full service facility with seven flow cytometers. We provide flow cytometry services for analysis and sorting of cells, as well as training and expert consultation for project/experiment planning.•High speed sorters: Beckman MoFlo; new cell sorter soming soon•Bio-Rad Bio-Plex for multiplex quantitative assays•5 flow cytometry analyzers: new BD Fortessa, new Beckman Gallios

Types of analysis

Immunophenotyping, DNA content, cell cycle analysis, DNA ploidy analysis, apoptosis, phagocytosis, intracellular pH, calcium levels, oxidative burst, intracellular antigen measurement, total protein, cytokine detection, intracellular signaling and isolation of rare cell populations including human and animal stem cells.

Flow Cytometry Service FCSBalaji Ganesh, Director

Staff

Jewell Graves, Lab Manager

Juan Chen, Research Specialist

Lakshmy Ganesh, Research Specialist

Appointments can be made for instrument instruction, sorts, and use of our flow cytometers on the FCS Online scheduler proteomics.rrc.uic.edu/flow.

Raw data and analysis files will be sent on line to with the "MY DATA" server.

Beckman Coulter Cyan ADP

Bio-Rad Bio-Plex

Beckman Coulter MoFlo

Becton Dickinson Fortessa

Transgenic Production Service

• DNA Microinjection/Transgenics• ES Cell Microinjection/Chimeric mice• ES cell expansion• Gene Targeting in ES cells• Embryo & Sperm Cryopreservation• Recovery of Pups from Cryopreserved

Embryos• Recovery of Pups from Cryopreserved

Sperm• In Vitro Fertilization (IVF)

The Transgenic Production Service provides a resource for investigators seeking to generate transgenic and gene-targeted mice for their research activities.

Animal Imaging and phenotyping

The Xenogen IVIS® Spectrum in vivo imaging system uses optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals.

Preclinical MRI Facility: 2,000 sq. ft. animal imaging laboratory equipped with a new Agilent 9.4 Tesla MRI system that is able to image small animals and samples up to 7.5 cm in diameter.

CCVR:Surgical services: Trans aortic constriction; Coronary artery ligation; Osmotic plant implantationImaging: VisualSonics Vevo770 ultrasound

Services Available

Tissues embedded in paraffin and processed for sectioning

Training and consultation available for by phone, e-mail or in person.

Antibody testing for determination of optimal conditions

Immunofluorescence of cultured cells

Fluorescent or colorimetric detection of antigens by immunohistochemistry

TUNEL staining in fixed sections for apoptosis studies

Routine Hematoxylin and Eosin staining of tissue sections

Specialized stains, such as the Gomori Trichrome Stain

Sample preparation/OCT freezing for cryosectioning

Tissue preparation for electron microscopy

Imaging: Nikon E400 fluorescent microscope equipped with a SPOT color camera.

ContactAndy Hall www.rrc.uic.edu/rhc

Microm Vacutome Cryostat service, or can be reserved to do your own sectioning

Microm Vacutome Cryostat service, or can be reserved to do your own sectioning

3 microtomes:Do your own or we do the work for you

Routine histology staining or immuno-histochemical staining

Nikon Eclipse E400 Epi-fluorescence microscope,

Immunohistochemical localization of cFOS inthe baboon endometrium visualized with DAB. Ha

Dual staining of Connexin 26 (green) and cytokeratin (red) in the baboon endometrium.

Research Histology Core (RHC)Director: Andy Hall COMRB 1120

Biorepository• Institutional resource for processing and

storing human tissue samples for research purposes.

• New LN and -85 freezers house blood, serum, plasma, urine, nucleic acids, & PBMC specimens

• IRB-approved opt-in protocol

• Specimens are banked in compliance with IRB-approved protocols and HIPAA standards

• Investigator protocols; 10 current, others in IRB

SERVICES INCLUDE:

•Separation of whole blood derivatives serum, plasma, PBMCs, urine, and saliva.

•Nucleic Acid isolation using iPrep™

•Opt-in or PI protocol; all samples bar coded and annotated

•Specimen management database moving from caTissue to OnCore

•Secure storage of samples/data

STAFFING:

•Director•2 Pathologists

• 2 Path Assistants• 2 Techs

BioInformatics Service

For analysis of big datasets from Genomics/Sequencing Resources

Integrated with Genomics, computing IT and Biorepository

Staff:• 4 Bioinformaticians specializing in analysis of

genome sequencing, gene expression, genotyping, pathways

Computing resources• Mixed cluster (GPU/CPU)• HPC coming soon• UIUC computing resources

New 3,000 sq ft facility in CME

Director: Ives Lussier, Technical Director: Neil Bahroos

Center for Genomic ResearchStaff: Co-Directors, 8 full time technicians, 4 students

Budget: $1.6M/yr

Services: • Next-Generation Sequencing• CE Sequencing• Quantitative analyses- QPCR

and Digital PCR• Automated Nucleic Acid purification

and QC• Gene expression analysis • High Throughput Genotyping, CNV• Cytogenomics

Select Capabilities Whole genome sequencing (De novo and re-sequencing)

• Ion Torrent Proton and PGM (UIC)

• Illumina HiSeq2000, Roche 454 (UIUC)

Target capture (custom/cancer panel/exome)

• Agilent SureSelect / Haloplex (hybridization capture)

• Ion Torrent Ampliseq / Qiagen Generead (PCR-based)

ChIP-seq: Sample prep with Covaris acoustic shearing

Microbiome Analysis: Ribosomal RNA gene sequencing

Genotyping: Affymetrix Gene Titan: 96 sample 2.2M microarray

• Sequenome: 768 sample throughput

Transcriptome sequencing

Differential gene expression

• Detection of novel transcripts

• Illumina HiSeq / Ion Torrent Proton

MicroRNA: Ion Torrent Proton; TILDA microfluidic arrays

Quantitative mRNA expression analysis

• Affymetrix Quantigene Plex: 3 to 80-plex; 96-sample throughput

• Affymetrix micro array

Cytoscan Affymetrix cytogenetic profiling

Mass Spectrometry, Metabolomics and Proteomics

Waters Synapt QTOF MS; ion mobility

Richard van Breemen, Academic Director; Alex Schilling, Technical Director Primary Staff: Rod Davis, Jerry White

2 Agilent 6410 Triple quadsChipCube system

Thermo LTQ-FT LC/MS/MS

Thermo Velos Orbitrap Pro funded by NCRRFitted with Chip Cube

•Mascot & SEQUEST search engines •Scaffold report generation: easier to interpret, interact with your results•Self-serve informatics workstations BioWorks and file conversion tools•X!Tandem searches•Specialized tools, customized databases

Services Provided• Accurate Mass Measurement• LC-MS and LC-MS-MS• MALDI MS and MS-MS• Quantitative Analysis• Structure Elucidation• Protein Identification• Metabolite Identification

Other Instrumentation• New Triple Quad ABSciex 5500• Shimazdu IT-TOF MSn

• JOEL GC-MATE MS• Thermo TSQ Quantum QQQ MS-MS• Agilent HPLC Chip Cube Interface• Agilent 1946C MSD MS• Thermo LCQDeca MSn

• Waters SCF-MS• Shimazdu LCMS 8030 QQQ MS-MS• Agilent Off-gel • Thermo LTQ MSn

Informatics Tools

The Scientific Instrument Shop (SIS) is staffed by Instrument Makers, Senior Laboratory Mechanics, and Biomedical Electronics Engineers who construct and repair all types of mechanical and electronic/electrical equipment for the research, teaching and clinical requirements of faculty, staff and students.

Staff

Electronics Shop: complete electronic services including the maintenance and repair of electronic devices. We also design and fabricate customized electronic equipment that may not be commercially available.

Eric Schmidt [email protected]

John Grega, Biomedical Engineering Technician

Matthew Schuck, Instrument Maker

Machine ShopSkilled Instrument Makers will assist designing and/or fabricating items utilizing the latest CAD/CAM software; full 3-axis and 3-D contour milling.

Materials: stainless and alloy steels, titanium, lead, platinum, brass, aluminum, copper, sheet metalplastics: acrylic, acetate, acetal, polypropylene, polystyrene, nylon, PVC, teflon, polycarbonates, polyethylene, engineered plastics wood, wood composites and machinable ceramics.

Capabilities:Turning in lathe up to 15" Manual milling 2 axes and 3 axes CNC milling Surface grinding Precision thread cutting Sheetmetal bending Forming, shearing and fabrication Hole punching Woodworking — sawing and fabrication

Scientific Instrumentation ShopDirector: Eric Schmidt

ServicesTig welding of most metals Brazing silver solder Sandblasting Thermocouples Repair and rebuilding Fabrication of parts- most plasticsmachining, plastic welding/gluing,bending and polishing

Contact US

William HendricksonDirector, RRCPhone: 312-996-5600Email: [email protected] Website: http://www.rrc.uic.edu/

Questions?

Biologic Resources Laboratory(BRL)

Biologic Resources Laboratory

• UIC’s Centralized Animal Research Facility• Oversight of the institution’s animal care and

use program• Support research• Daily care and husbandry of all laboratory animals• Procurement of all animals• Ensure animal welfare • Ensure compliance with regulatory agencies• Ensure the health status of animal colonies• Train students, staff, and faculty


•UIC’s Animal Care and Use Program has been continually accredited by AAALAC International since 1970.

•Oldest and most successful training programs for laboratory animal veterinarians

•Active professional staff • Professional organizations• Teaching• Research• Service

Biologic Resources LaboratoryProgram Scope

•137,000 sq. ft.•10 satellite facilities•House over 20 species•Ave. daily census 32,000 animals

• Mice 30,000• Primates 175

Biologic Resources LaboratoryProgram Scope• Staff - 64

• 11 vets – 5 board certified, 6 post-docs• 8 veterinary technicians• 37 husbandry staff• 6 administrative staff • 1enrichment specialist• 1 machinist

Biologic Resources LaboratoryAnimal Based Research Scope

•275 investigators•700 active research protocols•Types of studies

• Cancer• Immunotherapy• Radiobiology• Infectious disease• TransplantationHeart Disease• Diabetes• Neurologic disease• Retinal diseases

Biologic Resources Laboratory Services

• To gain access to any of the facilities• Listed on an approved ACC protocol• Orientation with one of the veterinarians• Visit employee health and enroll in the

occupational health and safety program

Biologic Resources Laboratory Services• Centralized surgery• Diagnostic laboratory• Radiology section• Antibody production service• Cross foster rederivation program • Support Good Laboratory Practice Studies• Support bio and chemical hazard work• Irradiator• Metabolic cages• Rodent quarantine• Resource


• Main phone number 312-996-7040• Website http://www.brl.uic.edu/

• Key contacts• Fee schedule

Animal per diem rateDiagnostic lab servicesSurgery services

• Jeffrey Fortman, Director Biologic Resources Lab

• 312-996-1220• [email protected]

Questions?