Exam 3 (mean 76%)
A 70-63
B 62-56
C 55-49
D 48-42
F 41-0
• Today-Ch 13
• Announcements – Look for Chapter 12 and 13 quizzes
posted this weekend.– Exam 4 covers Chapters 12, 13 and 16 (last
exam you can drop. Everyone must take the final exam)
Biology 190Chapter 16 pictures
DNA Replication occurs at the “S” stage of mitosis
Fig 5.26 Nucleotides
U
ACCCCAG
Fig 16.5 One DNA strand.
Fig 16.6 Double HelixACCCCAG
-T-G-G-G-G-T-C
Chargaff’s Rule
Question 16.1
Functions of Proteins
• Structural• Catalytic activity• Immunity• Regulation • Poisons• Transport molecules• Hormones• Fluid and electrolyte balance • Sources of energy
Fredrick Griffith’s Experiments
Polysaccharide Coat
Fredrick Griffith’s Results
Live R-strainpicked up DNA from the S-strain. Contains genes for “smooth coat”.
Alfred Hershey and Martha Chase
How viruses (phages) infect bacteria
Fig 16.4 Hershey and Chase
Watson and Crick
Rosalind Franklin’s Data(Maurice Wilkins’ Lab)
What exactly IS the structure of DNA?
Chargaff’s Rule
Nucleotide base pairing
Fig 16.7 Structure of DNA
Nobel Prize- 1962Wilkins Crick Watson
Figure 16.9 Semiconservative Model for DNA Replication
Parental strands(template strands)
Daughter strands
DNA Origin of Replication
Bacteria have one origin of replication.
Fig 16.12 Eukaryotic organisms have multipleorigins of replication.
Unwinding DNA
Single-strandedBinding Proteins
Nucleoside Triphosphate
Fig 16.13 DNA Polymerase(Daughter strand) (Parent strand)
(Old strand)
P
Questions 6.3 & 6.4
Fig 16.14ReplicationFork
Leading Strand
5’
3’
Lagging Strand
5’
3’
5’
5’
5’
5’
3’
3’
3’
3’
5’
5’
3’
3’
Old strandsParent strandsTemplate strands
Fig 16.14
DNA Ligase
DNA Polymerase III
Fig 16.13 DNA polymerase needs a 3’ -OH group
Fig 16.16
Fig 16.16. Review of Bacterial DNA Replication
Helicase
SS bindingproteins
Fig 16.18DNA shortening and telomeres
Mismatch repair
2 Purines = bulge
2 Pyrimidines = dimpleT C
G A
Fig 16.17 Excision Repair
Xeroderma Pigmentosa
In very, very, vary rare cases mutations have a
selective advantage
Recommended
