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Page 1: Effect of nitric oxide on the tolerance mechanism of bread wheat genotypes under drought stress

Abstracts / Journal of Biotechnology 185S (2014) S18–S36 S33

second population of P2O1 P2O3 and a more rapid rate of germi-nation. Germination of seeds P2O3 was more so within 15 days to28% sprouted seeds. After a 30-day stratification at 23–25◦ 25 days44–47% of the seeds germinated. Seeds of the first population needto start sprouting in a prolonged action of cold. If the seeds are firstpopulation stratification after 15 days gave 16%, then an increasein the duration of incubation of seeds in cold stratification for 30days until germination percentage increased to 34%. The resultsobtained on the biological characteristics of the ferrule Ili furtherserve as a basis for the development of recommendations for theconservation and restoration of populations Ferula iliensis.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.110

Implication of nitric oxide on growth anddevelopment of wheat under droughtconditions

Tijen Demiral 1,∗, Mehmet Hamurcu 2, ZuhalZeynep Avsaroglu 1, Merve Calik 2, Semra Almas 1,Erdogan Esref Hakki 2, Ali Topal 3, Sait Gezgin 2,Richard W. Bell 4

1 Department of Biology, Harran University, 63300Sanliurfa, Turkey2 Department of Soil Science and Plant Nutrition,Selcuk University, 42079 Konya, Turkey3 Department of Field Crops, Selcuk University,42079 Konya, Turkey4 School of Veterinary and Life Sciences, MurdochUniversity, 90 South St., Murdoch, 6150, Australia

E-mail address: [email protected] (T. Demiral).

Wheat grown in the Central Anatolian Region (CAR) depends onsurface and groundwater resources for irrigation but these areinadequate and increasingly costly to supply. Nitric oxide (NO)interacts with active oxygen species to prevent lipid peroxidationunder stress conditions. Thus, the potential alleviating effects ofNO on drought tolerance of a wheat genotype Karahan 99, whichis grown in CAR and selected for dry conditions were examined.Wheat plants were harvested 0 and 10 days after PEG 6000 treat-ment and the effects of exogenously applied SNP, a NO donor, ongrowth (root and shoot lengths, fresh and dry weights), Na, K, Caand Mg accumulation, proline content, ion leakage, lipid peroxi-dation and activities of antioxidant enzymes including superoxidedismutase (SOD), glutathione reductase (GR) and catalase (CAT)were determined. PEG-induced osmotic stress caused enhancedlipid peroxidation, higher H2O2 accumulation, and increased activ-ities of SOD and CAT while decreased GR activity in shoots. NOslightly decreased lipid peroxidation level, suppressed the activ-ities of SOD, CAT and GR enzymes and H2O2 accumulation moreunder osmotic stressed conditions.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.111

Effect of nitric oxide on the tolerancemechanism of bread wheat genotypes underdrought stress

Mehmet Hamurcu 1,∗, Tijen Demiral 2, MerveCalik 1, Z. Zeynep Avsaroglu 2, Ozgur Celik 2,Erdogan E. Hakki 1, Ali Topal 3, Sait Gezgin 1,Richard W. Bell 4

1 Department of Soil Science and Plant Nutrition,Selcuk University, 42079 Konya, Turkey2 Department of Biology, Harran University, 63300Sanliurfa, Turkey3 Department of Field Crops, Selcuk University,42079 Konya, Turkey4 School of Veterinary and Life Sciences, MurdochUniversity, 90 South St., Murdoch, 6150, Australia

E-mail address: [email protected] (M. Hamurcu).

Bread wheat is the dominant grain for Eurasia and Turkey whereit is prone to yield losses due to drought conditions. Two breadwheat (Triticum aestivum) genotypes, Kaplica and Goksu, weregrown hydroponically in a growth room and potential ameliorat-ing effects of exogenous nitric oxide (NO) treatment on droughttolerance of wheat genotypes were examined 10 days after PEG6000 exposure. Growth parameters (root and shoot lengths, freshand dry weights), proline accumulation, ion leakage and lipid per-oxidation level were measured also the activities of antioxidantenzymes including superoxide dismutase (SOD), catalase (CAT) andglutathione reductase (GR) and the accumulation level of H2O2and hydroxyl radical were determined. PEG 6000-induced osmoticstress caused oxidative stress by increasing lipid peroxidation andH2O2 accumulation, decreased GR but increased CAT activity ofboth genotypes. SOD activity of Kaplica was decreased while thatof Goksu was enhanced under stress. NO decreased lipid peroxida-tion, proline and H2O2 accumulation caused by osmotic stress anddid not evoke the activities of SOD, CAT and GR more than stresstreatment alone.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.112

Effect of nitric oxide on the tolerancemechanism of bread wheat genotypes undersalinity stress

Erdogan Esref Hakki 1,∗, Mehmet Hamurcu 1, TijenDemiral 2, Zuhal Zeynep Avsaroglu 2, MerveCalik 1, Mehmet Zengin 1, Ali Topal 3, SaitGezgin 1, Richard W. Bell 4

1 Department of Soil Science and Plant Nutrition,Selcuk University, 42079 Konya, Turkey2 Department of Biology, Harran University, 63300Sanliurfa, Turkey3 Department of Field Crops, Selcuk University,42079 Konya, Turkey4 School of Veterinary and Life Sciences, MurdochUniversity, 90 South St., Murdoch, 6150, Australia

E-mail address: [email protected] (E.E. Hakki).

Salt stress is one of the major abiotic stresses limiting cropgrowth and productivity worldwide. High salt conditions disturbion balance in plants; Na+ causes K+ deficiency and NO3

− uptakeis inhibited by excess Cl−. It is necessary to determine salinitytolerance mechanisms of plants and take measures to prevent dele-terious effects of salinity on plants. Nitric oxide (NO), which isinvolved in signal transduction pathways under stress conditions

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