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Abstracts / Journal of Biotechnology 185S (2014) S18–S36 S33 second population of P2O1 P2O3 and a more rapid rate of germi- nation. Germination of seeds P2O3 was more so within 15 days to 28% sprouted seeds. After a 30-day stratification at 23–25 25 days 44–47% of the seeds germinated. Seeds of the first population need to start sprouting in a prolonged action of cold. If the seeds are first population stratification after 15 days gave 16%, then an increase in the duration of incubation of seeds in cold stratification for 30 days until germination percentage increased to 34%. The results obtained on the biological characteristics of the ferrule Ili further serve as a basis for the development of recommendations for the conservation and restoration of populations Ferula iliensis. http://dx.doi.org/10.1016/j.jbiotec.2014.07.110 Implication of nitric oxide on growth and development of wheat under drought conditions Tijen Demiral 1,, Mehmet Hamurcu 2 , Zuhal Zeynep Avsaroglu 1 , Merve Calik 2 , Semra Almas 1 , Erdogan Esref Hakki 2 , Ali Topal 3 , Sait Gezgin 2 , Richard W. Bell 4 1 Department of Biology, Harran University, 63300 Sanliurfa, Turkey 2 Department of Soil Science and Plant Nutrition, Selcuk University, 42079 Konya, Turkey 3 Department of Field Crops, Selcuk University, 42079 Konya, Turkey 4 School of Veterinary and Life Sciences, Murdoch University, 90 South St., Murdoch, 6150, Australia E-mail address: [email protected] (T. Demiral). Wheat grown in the Central Anatolian Region (CAR) depends on surface and groundwater resources for irrigation but these are inadequate and increasingly costly to supply. Nitric oxide (NO) interacts with active oxygen species to prevent lipid peroxidation under stress conditions. Thus, the potential alleviating effects of NO on drought tolerance of a wheat genotype Karahan 99, which is grown in CAR and selected for dry conditions were examined. Wheat plants were harvested 0 and 10 days after PEG 6000 treat- ment and the effects of exogenously applied SNP, a NO donor, on growth (root and shoot lengths, fresh and dry weights), Na, K, Ca and Mg accumulation, proline content, ion leakage, lipid peroxi- dation and activities of antioxidant enzymes including superoxide dismutase (SOD), glutathione reductase (GR) and catalase (CAT) were determined. PEG-induced osmotic stress caused enhanced lipid peroxidation, higher H 2 O 2 accumulation, and increased activ- ities of SOD and CAT while decreased GR activity in shoots. NO slightly decreased lipid peroxidation level, suppressed the activ- ities of SOD, CAT and GR enzymes and H 2 O 2 accumulation more under osmotic stressed conditions. http://dx.doi.org/10.1016/j.jbiotec.2014.07.111 Effect of nitric oxide on the tolerance mechanism of bread wheat genotypes under drought stress Mehmet Hamurcu 1,, Tijen Demiral 2 , Merve Calik 1 , Z. Zeynep Avsaroglu 2 , Ozgur Celik 2 , Erdogan E. Hakki 1 , Ali Topal 3 , Sait Gezgin 1 , Richard W. Bell 4 1 Department of Soil Science and Plant Nutrition, Selcuk University, 42079 Konya, Turkey 2 Department of Biology, Harran University, 63300 Sanliurfa, Turkey 3 Department of Field Crops, Selcuk University, 42079 Konya, Turkey 4 School of Veterinary and Life Sciences, Murdoch University, 90 South St., Murdoch, 6150, Australia E-mail address: [email protected] (M. Hamurcu). Bread wheat is the dominant grain for Eurasia and Turkey where it is prone to yield losses due to drought conditions. Two bread wheat (Triticum aestivum) genotypes, Kaplica and Goksu, were grown hydroponically in a growth room and potential ameliorat- ing effects of exogenous nitric oxide (NO) treatment on drought tolerance of wheat genotypes were examined 10 days after PEG 6000 exposure. Growth parameters (root and shoot lengths, fresh and dry weights), proline accumulation, ion leakage and lipid per- oxidation level were measured also the activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) and the accumulation level of H 2 O 2 and hydroxyl radical were determined. PEG 6000-induced osmotic stress caused oxidative stress by increasing lipid peroxidation and H 2 O 2 accumulation, decreased GR but increased CAT activity of both genotypes. SOD activity of Kaplica was decreased while that of Goksu was enhanced under stress. NO decreased lipid peroxida- tion, proline and H 2 O 2 accumulation caused by osmotic stress and did not evoke the activities of SOD, CAT and GR more than stress treatment alone. http://dx.doi.org/10.1016/j.jbiotec.2014.07.112 Effect of nitric oxide on the tolerance mechanism of bread wheat genotypes under salinity stress Erdogan Esref Hakki 1,, Mehmet Hamurcu 1 , Tijen Demiral 2 , Zuhal Zeynep Avsaroglu 2 , Merve Calik 1 , Mehmet Zengin 1 , Ali Topal 3 , Sait Gezgin 1 , Richard W. Bell 4 1 Department of Soil Science and Plant Nutrition, Selcuk University, 42079 Konya, Turkey 2 Department of Biology, Harran University, 63300 Sanliurfa, Turkey 3 Department of Field Crops, Selcuk University, 42079 Konya, Turkey 4 School of Veterinary and Life Sciences, Murdoch University, 90 South St., Murdoch, 6150, Australia E-mail address: [email protected] (E.E. Hakki). Salt stress is one of the major abiotic stresses limiting crop growth and productivity worldwide. High salt conditions disturb ion balance in plants; Na + causes K + deficiency and NO 3 uptake is inhibited by excess Cl . It is necessary to determine salinity tolerance mechanisms of plants and take measures to prevent dele- terious effects of salinity on plants. Nitric oxide (NO), which is involved in signal transduction pathways under stress conditions

Effect of nitric oxide on the tolerance mechanism of bread wheat genotypes under drought stress

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Abstracts / Journal of Biotechnology 185S (2014) S18–S36 S33

second population of P2O1 P2O3 and a more rapid rate of germi-nation. Germination of seeds P2O3 was more so within 15 days to28% sprouted seeds. After a 30-day stratification at 23–25◦ 25 days44–47% of the seeds germinated. Seeds of the first population needto start sprouting in a prolonged action of cold. If the seeds are firstpopulation stratification after 15 days gave 16%, then an increasein the duration of incubation of seeds in cold stratification for 30days until germination percentage increased to 34%. The resultsobtained on the biological characteristics of the ferrule Ili furtherserve as a basis for the development of recommendations for theconservation and restoration of populations Ferula iliensis.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.110

Implication of nitric oxide on growth anddevelopment of wheat under droughtconditions

Tijen Demiral 1,∗, Mehmet Hamurcu 2, ZuhalZeynep Avsaroglu 1, Merve Calik 2, Semra Almas 1,Erdogan Esref Hakki 2, Ali Topal 3, Sait Gezgin 2,Richard W. Bell 4

1 Department of Biology, Harran University, 63300Sanliurfa, Turkey2 Department of Soil Science and Plant Nutrition,Selcuk University, 42079 Konya, Turkey3 Department of Field Crops, Selcuk University,42079 Konya, Turkey4 School of Veterinary and Life Sciences, MurdochUniversity, 90 South St., Murdoch, 6150, Australia

E-mail address: [email protected] (T. Demiral).

Wheat grown in the Central Anatolian Region (CAR) depends onsurface and groundwater resources for irrigation but these areinadequate and increasingly costly to supply. Nitric oxide (NO)interacts with active oxygen species to prevent lipid peroxidationunder stress conditions. Thus, the potential alleviating effects ofNO on drought tolerance of a wheat genotype Karahan 99, whichis grown in CAR and selected for dry conditions were examined.Wheat plants were harvested 0 and 10 days after PEG 6000 treat-ment and the effects of exogenously applied SNP, a NO donor, ongrowth (root and shoot lengths, fresh and dry weights), Na, K, Caand Mg accumulation, proline content, ion leakage, lipid peroxi-dation and activities of antioxidant enzymes including superoxidedismutase (SOD), glutathione reductase (GR) and catalase (CAT)were determined. PEG-induced osmotic stress caused enhancedlipid peroxidation, higher H2O2 accumulation, and increased activ-ities of SOD and CAT while decreased GR activity in shoots. NOslightly decreased lipid peroxidation level, suppressed the activ-ities of SOD, CAT and GR enzymes and H2O2 accumulation moreunder osmotic stressed conditions.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.111

Effect of nitric oxide on the tolerancemechanism of bread wheat genotypes underdrought stress

Mehmet Hamurcu 1,∗, Tijen Demiral 2, MerveCalik 1, Z. Zeynep Avsaroglu 2, Ozgur Celik 2,Erdogan E. Hakki 1, Ali Topal 3, Sait Gezgin 1,Richard W. Bell 4

1 Department of Soil Science and Plant Nutrition,Selcuk University, 42079 Konya, Turkey2 Department of Biology, Harran University, 63300Sanliurfa, Turkey3 Department of Field Crops, Selcuk University,42079 Konya, Turkey4 School of Veterinary and Life Sciences, MurdochUniversity, 90 South St., Murdoch, 6150, Australia

E-mail address: [email protected] (M. Hamurcu).

Bread wheat is the dominant grain for Eurasia and Turkey whereit is prone to yield losses due to drought conditions. Two breadwheat (Triticum aestivum) genotypes, Kaplica and Goksu, weregrown hydroponically in a growth room and potential ameliorat-ing effects of exogenous nitric oxide (NO) treatment on droughttolerance of wheat genotypes were examined 10 days after PEG6000 exposure. Growth parameters (root and shoot lengths, freshand dry weights), proline accumulation, ion leakage and lipid per-oxidation level were measured also the activities of antioxidantenzymes including superoxide dismutase (SOD), catalase (CAT) andglutathione reductase (GR) and the accumulation level of H2O2and hydroxyl radical were determined. PEG 6000-induced osmoticstress caused oxidative stress by increasing lipid peroxidation andH2O2 accumulation, decreased GR but increased CAT activity ofboth genotypes. SOD activity of Kaplica was decreased while thatof Goksu was enhanced under stress. NO decreased lipid peroxida-tion, proline and H2O2 accumulation caused by osmotic stress anddid not evoke the activities of SOD, CAT and GR more than stresstreatment alone.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.112

Effect of nitric oxide on the tolerancemechanism of bread wheat genotypes undersalinity stress

Erdogan Esref Hakki 1,∗, Mehmet Hamurcu 1, TijenDemiral 2, Zuhal Zeynep Avsaroglu 2, MerveCalik 1, Mehmet Zengin 1, Ali Topal 3, SaitGezgin 1, Richard W. Bell 4

1 Department of Soil Science and Plant Nutrition,Selcuk University, 42079 Konya, Turkey2 Department of Biology, Harran University, 63300Sanliurfa, Turkey3 Department of Field Crops, Selcuk University,42079 Konya, Turkey4 School of Veterinary and Life Sciences, MurdochUniversity, 90 South St., Murdoch, 6150, Australia

E-mail address: [email protected] (E.E. Hakki).

Salt stress is one of the major abiotic stresses limiting cropgrowth and productivity worldwide. High salt conditions disturbion balance in plants; Na+ causes K+ deficiency and NO3

− uptakeis inhibited by excess Cl−. It is necessary to determine salinitytolerance mechanisms of plants and take measures to prevent dele-terious effects of salinity on plants. Nitric oxide (NO), which isinvolved in signal transduction pathways under stress conditions